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Citations for Merck :
2751 - 2776 of 2776 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice with PBS and then incubated with freshly made SA-β-gal buffer containing 25 mM Na2HPO4 (Merck Life science), 7.4 mM Citric Acid (Merck Life science) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the frozen cell suspension was thawed and incubated with end-over-end mixing together with 1 mg/mL lysozyme (Merck KGaA, Darmstadt, Germany) for 30 min (all purification steps at 4 °C of on ice) ...
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bioRxiv - Cancer Biology 2023Quote: 1000 freshly sorted GFP+ and GFP- MM13 and MM27 cells were grown as hanging drops in IMDM complete medium (Sigma Aldrich-Merck, Cat#I3390) for 72 h at 37°C in normal (20%O2 ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Neuroscience 2023Quote: ... filtered to remove cell debris and particles were concentrated (30-fold) via low-speed centrifugation (Amicon Ultra-15, Ultracel-100; Merck Millipore; Cat# UFC9100) before aliquoting and storage at −70°C ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated for 1 h at room temperature with the monoclonal mouse Anti-Tom20 (1:500, MABT166, Merck & Co., Kenilworth, NJ, USA). The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
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bioRxiv - Physiology 2021Quote: ... The shRNA-containing vector was co-transfected with the pE-ampho vector into HEK293T cells using GeneJuice transfection reagent (Merck Millipore, Burlington, MA, USA). Supernatants containing viral particles were collected 48 h after the transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4° C ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Cell Biology 2022Quote: The cellular health of Eca EPCs Non-OB and Eca EPCs OB was evaluated using Guava® Muse® Cell Analyser (Sigma-Aldrich/Merck, Poznan, Poland). The metabolic activity of cells was determined based on mitochondrial membrane potential using Muse™ Mitopotential Assay Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... antibodies were added to each aliquot (equivalent to 100 ml of cell culture): 6μl anti- H3K56ac for the mid-S time point (Merck-Millipore, 07-677-IS (lot# 266732) or 10 μl anti- H3K56ac for the early-S time point (Active Motif ...
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bioRxiv - Cell Biology 2024Quote: ... rat neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL poly-D-lysine (PDL, Merck/Sigma-Aldrich #A-003-E). Mouse neurons were plated at a density of 13 to 19 x103 cells/cm2 on glass coverslips coated with 333 µg/mL PDL and 100 to 200 µg/mL laminin (Merck/Sigma-Aldrich #L2020) ...
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bioRxiv - Biochemistry 2023Quote: ... Fifty μl of a suspension in water of GA-fixed cells were mixed with 50 μl of 2% osmium tetroxide in water (Cod. 75632-5ML, Merck Life Science S.r.l., Milan, Italy). After 30 min at room temperature ...
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bioRxiv - Physiology 2023Quote: The pancreatic β cell line INS-1E was cultured in a humidified chamber with 5% CO2 in RPMI-1640 medium (Merck, Sigma Aldrish, Darmstadt-Germany) at 11.1 mM glucose supplemented with 5% (vol./vol.) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were dissociated by mincing and enzymatic digestion of the extracellular matrix using 500 µg/ml collagenase type 1a (Merck Life Sciences UK Ltd, Gillingham, U.K) and 100 µg/ml DNAse I (Lorne Laboratories Ltd ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Microbiology 2020Quote: ... as previously described (details in Supplementary materials).13–16 Organoid-derived monolayers (ODMs) were prepared in Matrigel-coated transwell cell culture inserts (Merck Millipore, 0.6 cm2, 0.4 μm pores). For this ...