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Citations for Merck :
2551 - 2600 of 2776 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Immunology 2023Quote: ... the streptavidin tetramer mixture (containing D4, A1D3, A2D2, A3D1 and A4) was concentrated in an ultrafiltration cell (10 kDa cut off, Merck); mSav (A106C ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Neuroscience 2023Quote: ... coated dishes at low confluency (5,000–10,000 cells/cm2) in MEF-conditioned hES medium supplemented with 10 μM ROCK inhibitor (Y-27632; Merck, Cat#688000). On DIV0 of the differentiation ...
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bioRxiv - Developmental Biology 2023Quote: ... Product number E4250) (1 mg/ml) to contract pigment cells alongside 0.01% Tricaine (Ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich (Merck) Product number E10521 ...
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bioRxiv - Immunology 2023Quote: ... Titers were determined by transduction of Jurkat cells (ACC 282; DSMZ) in presence of 5 µg/ml Polybrene Infection/Transfection Reagent (Merck). After 48 h ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: To achieve this FLK1+ HB cells were plated onto gelatine coated tissue culture flasks at a concentration of 1.6⍰×⍰104 cells per cm2 in Blast media (IMDM (Merck, I3390), 10% FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells from identical conditions were collected and pooled in 10 ul in calcium-free and magnesium-free HBSS (Merck, H8264) + 50% FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Clones were trial-screened for positive PCR products by making mirror plates and subjecting one of the plates to cell lysis (50mM Tris-HCl pH 8, 1mM EDTA, 0.5% Tween-20, 50-80 μg/ml Proteinase K Merck-3115801001) at 37°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Immunology 2023Quote: CORA receptors were transduced into a previously described Jurkat-based reporter cell line (12) by addition of lentiviral particles in a MOI of 1 and 5 μg/mL Polybrene (Merck) using spinoculation ...
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bioRxiv - Neuroscience 2023Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... WM983B cells were infected by lentivirus in the presence of 5µg/mL polybrene (ref. n°TR-1003, Merck/Sigma-Aldrich) followed by antibiotic selection (puromycin 1µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
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bioRxiv - Bioengineering 2023Quote: ... GFP-coding or GFP/Flagtagged nanobody constructs optimized for mammalian cell expression were transfected using the GeneJuice transfection reagent (Merck). 24 hours after transfection ...
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bioRxiv - Immunology 2023Quote: ... Cells were then acquired on the flow cytometer for ~25 seconds before the addition of 1 μg/mL ionomycin (Merck), and calcium flux was measured for ~1.5 mins.
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bioRxiv - Developmental Biology 2023Quote: ... 2-cell stage embryos were similarly recovered from hybrid superovulated females (using 5IU of PMSG and 5IU of hCG; Merck), generated after crossing BDF1 male and CD1 females (each strain obtained from Anlab ...
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bioRxiv - Cell Biology 2023Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and the resulting gel disc was mounted in a live imaging chamber (Aireka Cells) with low-melt agarose (A7431, Merck). The chamber was filled with ddH2O to prevent sample shrinkage and drying ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Molecular Biology 2023Quote: ... At 48 hours of transfection (If indicated) the cells were treated with the following conditions before harvesting at indicated times: 2mM of H2O2 (MERCK) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant of the transfected cells was collected 48 h later and filtered through a 0.45 μm pore-size filter (Merck Millipore). For viral infection ...
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bioRxiv - Immunology 2023Quote: ... Antibody-stained cells were subsequently probed with Duolink In Situ PLA Probe anti-mouse PLUS and anti-mouse MINUS probes (Sigma/Merck). Next ...
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bioRxiv - Cell Biology 2023Quote: ... the media of the cells was replaced with XF base media supplemented with 2 mM L-Glutamine (Merck Life science), 17.5 mM glucose (Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... after centrifugation the cell pellet was resuspended in 0.1 M HCL solution containing 0.5 mg/ml of the protease pepsin (Merck Life science) and left for 20 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Neuroscience 2023Quote: ... The next day cells were split for selection on 400 μg/ml collagen-IV and 100 μg/ml fibronectin-coated (both from Merck) Transwell inserts (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellet was resuspended in 400 μl of 40 μg/ml ultra-pure digitonin (Merck, CAS 11024-24-1) in vPBS and incubated (25 min ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell culture supernatants from 4950T or 170162T cells were concentrated 10x using Amicon centrifugal filtration units with 100 kDa cutoff filter (Merck). Concentrated supernatant or glycodelin A isolated from amniotic fluid was added to the lectin coupled beads and incubated overnight on an overhead shaker at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
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bioRxiv - Cell Biology 2024Quote: ... The supernatant containing the pre-cleared crude cell lysate was collected by attaching the tubes to a Magna Rack (Merck Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were then transferred to cell-culture plastic plates that had been previously treated with poly-L-lysine 10 μg/ml (Sigma-Merck) to enhance cellular adhesion and promote monolayer cultures ...
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bioRxiv - Neuroscience 2024Quote: ... and seeded at a density of 2.0 x 104 cells/cm2 on glass coverslips coated with Laminin/Poly-L-Ornithine (Sigma and Merck-Millipore), or at a density of 5.0 x 104 cells/cm2 on Laminin-/Poly-L-Ornithine coated plates in StemDiff Forebrain Neuron Maturation medium (Stem Cell Technologies ...
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bioRxiv - Immunology 2024Quote: ... 130 pg of gRNA and 250 pg of the Cas9 protein were co-injected into a 1-cell stage fertilized embryos of AB WT fish with phenol red (pn. 114537-5G, Merck) as a tracer dye ...
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bioRxiv - Developmental Biology 2024Quote: ... Citrine+ cells were enriched via FACS using a BD Influx cell sorter with a 70um nozzle into DNA Lobind 1.5mL Eppendorf tubes (Merck, EP0030108051), spun post sort at 400g ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... grown in semi-suspension in 150 mm plates in a mixture of serum free media (ACF CHO medium EX-CELL Merck, CHO ONE media Capricorn Scientific ...
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bioRxiv - Immunology 2024Quote: ... Viral supernatant was filtered and used for transduction of NALM6 and K562 cells through spinoculation together with 8 μg/ml protamine sulfate (Merck). Transduced cells were selected using puromycin (1 μg/ml).
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bioRxiv - Neuroscience 2024Quote: Transfected cells were solubilized in gel loading buffer and analyzed through electrophoresis before being transferred to nitrocellulose membranes (Merck Millipore). Immunodetection was achieved with the primary antibodies (1:1000 in blocking solution) ...
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bioRxiv - Microbiology 2024Quote: ... and human embryonic kidney (HEK) 293T cells (CRL-3216; ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Merck, Darmstadt, Germany) with 10% FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue slices were dissected under the microscope in ice cold PBS before they were transferred on top of hydrophilic Millicell cell culture inserts (Merck Millipore) into serum-free Advanced Dulbecco’s modified Eagle’s medium F12 supplemented with 0.5% N2 ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lymphocytes were isolated from the spleen and incubated in a tissue culture tube at a cell concentration of 1 × 106/ml in RPMI1640 (Nacalai Tesque) containing lipopolysaccharide (10 μg/ml, Merck KGaA), concanavalin A (3 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed using ice-cold RIPA buffer containing 1% of protease and phosphatase inhibitor cocktail (Sigma Aldrich/Merck, Poznan, Polska). The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck ...
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...