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Citations for Merck :
2651 - 2700 of 2776 citations for Neural Precursor Cell Expressed Developmentally Down Regulated 9 NEDD9 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1X Protease inhibitors and 1X Phosphatase inhibitors (PPI)] plus 2μL per sample Pierce Universal Nuclease for Cell Lysis and 1μL per sample Benzonase® Nuclease (Merck, E1014-25KU) for 30min at 4°C on wheel with vigorous pipetting every 10min ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The purity of the cultures was be routinely assessed by examining the characteristic cell morphologies under phase-contrast microscopy and confirmed by immunostaining with mouse anti-GFAP (1:200; Merck, MAB3402) and mouse anti-S100β (1:400 ...
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bioRxiv - Cell Biology 2023Quote: Tumor slice and ear derma explants were then cultured on a 30-mm cell culture insert featuring a hydrophilic PTFE membrane (0.4 µm pore size, Merck Millipore #PICM0RG50) placed inside 6-well plates containing 1.1 mL of RPMI ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2023Quote: ... and the trophozoites were resuspended in 500 μl NaPBS/0.05 % saponin (or permeabilization of cell membranes (Sigma-Aldrich, Merck, Taufkirchen, Germany) and incubated for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM medium (Merck/Biochrom) supplemented with 10% HI-FBS (Merck/Biochrom) ...
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bioRxiv - Biochemistry 2023Quote: ... 150,000 to 200,000 HEK293-EBNA cells were plated in 1 mL complete DMEM per well of 12-well cell culture plates (#665180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... CellBiologics) were cultured in a complete Endothelial Cell Medium (CellBiologics) in petri-dish pre-coated with 0,2% gelatin (Merck Life Science). Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were grown until confluence in 0.2 mg/ml collagen-type I-coated 6 well plates (Merck, Darmstadt, Germany) and incubated in serum-free medium for 24 h with/without Dox treatment ...
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bioRxiv - Developmental Biology 2023Quote: ... feeder depleted mESCs were seeded for EB formation at 25,000 cells/ml in ESGROTM Complete Basal Medium (ESGRO, Merck, SF002-500) in non-adhesive 60 mm or 100 mm culture dishes ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered cell culture supernatant containing infectious HCV was concentrated 50x using 100 kDa Amicon Ultra-15 centrifugal filter units (Merck Millipore) as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue fragments (‘organoids’) released were incubated for 5 min in Red Blood Cell Lysis buffer (Merck Sigma-Aldrich, Gillingham, Dorset, UK), washed and then plated for 1 hr at 37°C in DMEM/10%FBS (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: Wild type and PREPL KO HEK293T cells were grown on glass coverslips coated with Poly-D-Lysine (Merck A-003-E) and fixed with 4% formaldehyde ...
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bioRxiv - Cell Biology 2024Quote: ... and the pellet containing the cells was resuspended in 1 mL PBS supplemented with protease inhibitor (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck). The cells were lysed by 20 passages through a bead homogenizer (16 µm bead ...
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bioRxiv - Cell Biology 2024Quote: ... AT2 Organoids were fixed with PFA for 3 minutes and then released from matrigel by incubating the culture with Corning Cell Recovery Solution (Merck, CLS354253) for 2 hours at 4 °C.
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bioRxiv - Microbiology 2024Quote: ... algal cells concentrations from a late exponential phase culture were counted by a CellStream CS-100496 flow cytometer (Merck, Darmstadt, Germany) using 561 nm excitation and 702 nm emission ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were then washed in cold 50 mL cold PBS cOmplete™ EDTA-free Protease Inhibitor Cocktail tablets (Merck, #73567200) and centrifuged at 2000 rpm for a further 10 minutes ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor at 30°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Microbiology 2024Quote: Human small lung carcinoma A549-AT cells stably expressing ACE2 and TMPRSS2-GFP73 and VeroE674 cells were maintained in Dulbecco’s Modified Eagle’s High Glucose Medium (DMEM, Merck, Cat. no. D6546), 1% L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Cell Biology 2024Quote: ... Thawed Matrigel was diluted 1/50 in cold E8 media and was then added to the cell culture dishes (Merck, #Z755923). Coated dishes were incubated at room temperature for 1 hour and used immediately or kept in the fridge for later application.
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Microbiology 2021Quote: Embedding in Lowicryl K4M (Chemische Werke Lowi, Waldkraiburg, Germany) was carried out on Vero cells fixed either in 4% formaldehyde (Merck, Darmstadt, Germany) or in 2% glutaraldehyde at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... the SH-SY5Y cells were collected and the ROS levels were detected using the fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA). Cells were incubated with 5 µM DHE dye for 20 min at 37°C in the dark and then the fluorescence intensity was measured by flow cytometry in the PE channel ...
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bioRxiv - Genomics 2020Quote: ... 10 × 106 cells were used per IP and all lysis and wash buffers were supplemented with Roche cOmplete™ protease inhibitors (Merck, 11873580001) and Pierce phosphatase inhibitors (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were allowed to adhere overnight (16 h) in the presence of 2 mM Thymidine (S-phase block; Merck, Darmstadt, Germany) and released into fresh medium ...
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bioRxiv - Bioengineering 2020Quote: ... the digests were separated by an Amicon® Bioseparations Stirred Cells device over a 1kDa ultrafiltration disc (Merck Millipore; Amsterdam, The Netherlands) into a retentate and filtrate ...
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bioRxiv - Microbiology 2019Quote: ... total GLP-1 levels in cell supernatants were measured using High Sensitivity GLP-1 Active ELISA-Chemiluminescent (Merck Millipore, EZGLPHS-35K, Germany) according to the reports (38 ...
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bioRxiv - Genetics 2019Quote: ... Chromosome spreads were prepared by dropping the cell suspension on a grass slide and stained with 5% Giemsa solution (Merck Millipore, Germany) for 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Fixed cells were washed with cold PBS and centrifuged at 2000 x g for 5 minutes and stained using a Muse™ Cell Cycle Kit (Merck Millipore) for 30 minutes in dark conditions ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 mL of fixed cell suspension was filtrated by 0.22-µm pore-sized black polycarbonate filter (type GTBP; Merck Millipore, Darmstadt, Germany) and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain ...
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bioRxiv - Immunology 2019Quote: BlaER1 cells (WT and TLR8 -/-, kindly provided by Tatjana Eigenbrod from Heidelberg) were cultured in culture medium (VLE-RPMI (Merck Biochrom, FG1415) + 10 % FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were incubated with the reagent at room temperature for 30 minutes in the dark and run through the Muse Cell Analyzer (Merck, Darmstadt, Germany) with 10,000 events per sample and analyzed with the Muse Cell Analyzer software ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Neuroscience 2021Quote: ... Axonal fraction and the fraction containing neuronal body with dendrites were isolated by carefully detaching the cellular contents from the inner and outer insert membrane surface with a cell scraper in TAEB buffer (Merck, Darmstadt, Germany) four times ...
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bioRxiv - Cell Biology 2021Quote: ... Retinal pieces were cultured on a cell culture insert with a 0.4 µm pore diameter (Millicell PICM0RG50, Merck Millipore Ltd, Cork, Ireland) in DMEM/Ham’s F-12 without Phenol Red (Nacalai Tesque ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...