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Citations for Merck :
2551 - 2600 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... For super-resolution microscopy, samples were incubated with secondary antibodies (STAR635P (Abberior, 1:200) and Atto594 (Merck KgaA, 1:200)) ...
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bioRxiv - Immunology 2024Quote: ... 2010) that recognizes a common epitope on MASP-1,-3 and MAP-1 followed by HRP-conjugated streptavidin (Merck, RPN1231). The plates were revealed using TMB One as the substrate (Kementec ...
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bioRxiv - Immunology 2024Quote: ... and passaged two times on MDCK-II cells with FBS-deficient medium containing 1% penicillin/streptomycin and 1 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone-treated trypsin (TPCK-trypsin, Merck KGgA ...
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bioRxiv - Cancer Biology 2023Quote: ... one mouse was exposed by drinking water to a mixture of BPA (1 µM, CAS no. 80-09-1, Merck) and DEHP (1 µM ...
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bioRxiv - Immunology 2023Quote: 200 μg of HLA.A2-peptide complex in PBS was concentrated to 1 mg ml−1 with the use of Amicon Ultra Centrifugal Filters (Merck), adjusted to pH 8.3 by adding freshly prepared NaHCO3 (0.1 M final concentration ...
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bioRxiv - Immunology 2023Quote: ... peptides were dissolved at a concentration of 1 to 5 mg ml-1 in 1x PBS and incubated with TCEP agarose CL4-B (Merck) for 1 hour to reduce paired cysteines ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated with an anti-sera peptide based polyclonal rabbit GLK2-antibody (1:3,000) and a peroxidase-conjugated anti-rabbit antibody (1:10,000) (Merck, Darmstadt, Germany) for 1 h at room temperature in TBST [Tris-HCl 20 mM (pH 7.6) ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on manufacturer-suggested minimum seeding density) with lenti/retroviral supernatant in a 1:1 volumetric ratio and 8 µg/mL hexadimethrine bromide (Merck), before centrifugation at 900g for 30 min and returning to incubation at 37°C with 5% CO2 ...
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bioRxiv - Plant Biology 2024Quote: ... Waters Inc.) was conditioned with 1 mL of 100% methanol and 1 mL of deionized water (Milli-Q, Merck Millipore). After the conditioning steps each sample was loaded on SPE column and flow-through fraction was collected with the elution fraction ...
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bioRxiv - Biochemistry 2024Quote: ... Nuclease reaction products were separated at a flow-rate of 1 ml*min-1 on a Chromolith Performance RP-18e 100 x 46 mm column (Merck) equipped with a guard cartridge ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was diluted in 10 ml of 1X PBS buffer and filtered using an Amicon 50 kDa filter column (Merck, Darmstadt, Germany) to concentrate the extract to a final volume of 500 μL.
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were normalized according to the number of worms and applied to 10 cm HPTLC silica gel 60 matrix plates (Merck, Darmstadt, Germany). For lipid analysis ...
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Bioengineering 2020Quote: ... in an ice bath for 100 cycles (1 cycle comprised 20 sec ultrasonication and 10 sec cooling) and filtrated (40 µm filter, microsyringe 25 mm filter holder, Merck, Darmstadt, Germany), before being freeze-dried for 48 h (Freeze dryer FDU-2200 ...
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bioRxiv - Biochemistry 2021Quote: ... On the next day the buffer was exchanged to IEX-A buffer (25 mM Bis-Tris, 50 mM NaCl, 10 mM. β-mercaptoethanol) by ultra-filtration (Amicon Ultra-15 30 kDa, Merck Millipore) and subject to cation-exchange chromatography by a linear to IEX-B buffer (25 mM Bis-Tris ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then cells were transferred to a 96-well plate and thereafter challenged with antibiotics at a concentration of 2×MIC either with or without lipid II 51 (10 μM; provided by Prof. Eefjan Breukink)/LPS (100 μg/mL; Merck, L2880-100MG). The absorbance values were measured by using a Varioskan™ LUX multimode microplate reader (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... 1000 μl tips were fitted with 2 plugs of C18 octadecyl 47mm Disks 2215 (Empore™) material and 10 μg of LiChroprep® RP-18 peptides (Merck). Tips were sequentially equilibrated with 100 % methanol ...
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bioRxiv - Microbiology 2021Quote: ... The viral particles were suspended in 10 ml phosphate-buffered saline (PBS) and filtered using a 0.45-μm filter (Millex-AA, Merck Millipore, Darmstadt, Germany). The filtered viral particles were centrifuged (8,500×g ...
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bioRxiv - Microbiology 2021Quote: ... and 10% ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 12 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... fillet dissections of L3 larvae or pharate adults were performed in modified HL3 solution ([HL3-EGTA] 70 mM NaCl, 5 mM KCl, 10 mM NaHCO3, 20 mM MgCl2, 5 mM trehalose (Merck KGaA, 108216), 115 mM sucrose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... spherical assembloids were collected in a PCR tube and immediately incubated in BBB working medium with 10 μM 4 kDa dextran-FITC (FD4; Merck, cat. #46944) or 10 μM fluorescently labeled human transferrin (Tf488 ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: ... fixed with paraformaldehyde 4 % solution for 10 min and stained with a solution containing PBS - 0.5% crystal violet (Sigma-Aldrich/Merck, ref: C3886) – 25% methanol for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 50 mM NaCl buffer (pH 7.0) with ultrafiltration using Amicon® Ultra-15 Centrifugal Filter Unit (10 kDa MWCO) (Merck, Darmstadt, Germany). With the prepared 100 × SYPRO® Orange solution and the protein solution ...
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bioRxiv - Microbiology 2021Quote: ... and 10 % Ultra-pure water from Direct-Q® 3 UV Water Purification System with LC-Pak® Polisher (Merck KGaA, Darmstadt) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Biophysics 2019Quote: ... aSyn was extensively dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA, Darmstadt, Germany) and stored at −80 °C until use ...
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bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The tissue was processed as previously reported [26] and proteins were loaded onto 10% polyacrylamide gels and transferred to PVDF sheets (Immobilion-P, MERCK, Burlington, USA). Membranes were blocked with BSA 5% and immunoblotted using primary antibodies for β-Tubulin ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fractions with the highest β-glucosidase activity or GFP or CFP fluorescence were pooled and applied to an Amicon Ultra centrifugal filter unit with 10 kDa cutoff (Merck Millipore, USA) for protein concentration and buffer exchange (TBS with 10 mM CaCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of sample was injected in 50 % B and the analysis was performed using the gradient of 15 % A and 85 % B for 3.5 min followed by an increase to 75 % A and 25 % B over 11.5 min and its continuation for 10 min with the SeQuant® ZIC®-pHILIC column (5 µm, 150 mm x 2.1 mm, Merck, USA), at a flow rate of 0.13 ml/min.
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bioRxiv - Molecular Biology 2019Quote: ... The membranes used as accumulation wall were comprised of regenerated cellulose with a molecular weight cut-off of 10 kDa (Merck Millipore DE). Flows were controlled with an Agilent Technologies 1200 series isocratic pump equipped with vacuum degasser ...
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bioRxiv - Cell Biology 2019Quote: ... the dye was removed by buffer exchange using a spin concentrator (Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-10 membrane (Merck Millipore, UFC901024), and a buffer containing 20 mM HEPES (pH 7.4) ...
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bioRxiv - Biophysics 2021Quote: ... aSyn was extensively dialysed against H2O and used immediately for experiments or dialysed against 20 mM Tris pH 7.2 and concentrated using 10 k MWCO amicon centrifugal filtration devices (Merck KGaA, Darmstadt, Germany) and stored at −80 °C until use ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Immunology 2021Quote: ... These fractions were then pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096). The clinically relevant mAbs tested here were in-house produced versions of ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin™ dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (5).
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was then supplemented with 5 mL of 0.9% NaCl and concentrated by centrifugation (4,000 × g; 10 min; room temperature) using Amicon Ultra-15 100 kDa centrifugal filtration units (regenerated cellulose; Merck/Millipore, Cork, Ireland). To further reduce the protein content ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was finally washed again in PBS three times for 10 minutes before mounting using FluorSave mounting medium (Merck-Millipore #345789). For immunostainings against MBP ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail [5 μl/ml] and phosphatase inhibitor cocktail [10 μl/ml]) and 0.5% Nonidet P-40) (I8896; Sigma-Aldrich/Merck group, USA). After 10 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated in the dark for 10 minutes before analysis by flow cytometry using the Guava® easyCyte™ 5HT HPL Benchtop flow cytometer (Merck). Data were analysed using FlowJo™ v10.2 Software (BD Life Sciences).
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Microbiology 2022Quote: ... TBS buffer (50 mM Tris, pH 7.5, 10 mM EDTA) was filter-sterilized using a 0.22 μm filter (Merck Millipore Ltd, Burlington, MA) and kept at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... disposable 200 μl pipette tips were fitted with 2 plugs of C18 octadecyl 47 mm Disks 2215 (Empore™) material and 1mg:10 μg of LiChroprep® RP-18 (Merck) ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...