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Citations for Merck :
2451 - 2500 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer exchanged with SnakeSkin™ dialysis tubing (10 MWCO, Pierce, 68100) followed by additional dialysis and concentration with Amicon Ultra-15 50 kDa centrifugal units (Merck, UFC905008). Purified protein was conjugated to AlexaFluor647 (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Genomics 2022Quote: ... During sampling we set a flow rate of 200 l/minute for a total of 60 minutes with 10 ml 0.05 % (v/v) Triton X-100 (T8787-50ML, Merck Sigma Aldrich) as collection buffer prepared with UltraPure DNase/RNase free ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was re-applied onto Ni-NTA Superflow resin and the flow-through was pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was re-applied onto the Ni-NTA Superflow cartridges and the flow-through was pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S75 (26/600 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was removed (around 25 ml) and either concentrated 10-fold with Amicon 3 kDa spin columns (Merck, cat. #UFC900308) and brought to a final volume of 2 ml for subsequent Western blot analysis (Fig.1B ...
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bioRxiv - Plant Biology 2023Quote: ... a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa, Merck).
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bioRxiv - Cancer Biology 2023Quote: ... A total of 50 μg of proteins were separated by 10% or 15% SDS- polyacrylamide gel (SDS-PAGE) and transferred to a nitrocellulose membrane (Merck, HATF00010). To detect the CXCL16 and CXCL8 secreted in the culture medium ...
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: ... female mice aged 6-8 weeks were stimulated to ovulate by 10 IU intraperitoneal injection of Foligon/PMSG (MSD Animal Health, Spain) followed by intraperitoneal administration of 10 IU of Ovitrelle 250 µg/0.5 mL (Merck Serono, Germany) 48 h later ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... the eluate was reduced to approximately 40–50 ml and then concentrated using an Amicon Ultra-15 10 kDa molecular mass cut-off spin filter (Merck Millipore). Final EV preparations were kept in sterile filtered PBS for immediate use or sterile PBS-HAT buffer56 for storage at −80℃.
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Cell Biology 2024Quote: ... The next morning cells were transfected with 10 µg of the plasmid of interest using GeneJuice transfection reagent (Merck, #70967-3) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were resolved on a 10% SDS-PAGE gel and Western blotting was performed using an anti-myc antibody (Merck, UK). Co-immunoprecipitation of Ssk2-HA was detected using an anti-HA antibody (Merck ...
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bioRxiv - Biophysics 2024Quote: ... The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250, Merck) to confirm its purity ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in a 10-cm-diameter tissue culture dish (IWAKI) with BIS medium under an anaerobic condition using Anaerocult® A (Merck). Trophozoites were detached from the surface of the dishes by adding cold PBS into the dishes and incubating them at 4℃ for 10 minutes ...
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The node of Ranvier influences the in vivo axonal transport of mitochondria and signalling endosomesbioRxiv - Neuroscience 2024Quote: ... with 7.5-10 μg of HCT in PBS in a volume of ∼3.5 µl using a 701 N Hamilton® syringe (Merck, 20,779) for TA ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Biophysics 2024Quote: ... The isolated protein was concentrated using Amicon® Ultra centrifugal filter with a molecular cutoff 10 kDa (Amicon Ultra-4 Centrifugal filter unit 10K, Merck). The SEC peak fraction for recombinant α-HL was further loaded into 10% SDS-PAGE (Coomassie Brilliant blue R 250 ...
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bioRxiv - Microbiology 2024Quote: Asexual spores of the strain HEK1 were collected from a fully colonized plate (10 cm diameter) with 15 mL of Tween® 80 (0.016%) (Merck, DE). The resulting suspension was centrifugated (Sigma 2-16PK ...
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bioRxiv - Biochemistry 2024Quote: ... equipped with a capillary column (SUPELCOWAX™ 10; 30 m x 0.32 mm i.d. x 0.25 μm, Merck KGaA, Darmstadt, Germany). The injection port and ion source temperatures were 250 °C and 200 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... then incubated with primary antibodies (mouse anti-PAR, 1:400 Merck Millipore Cat#AM80 RRID: AB_2155072; mouse anti-γH2AX pS139, 1:2000 Merck Millipore Cat#05-636 RRID ...
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bioRxiv - Plant Biology 2020Quote: ... The Falcon tube systems consisted of a 50 ml Falcon tube filled with 37.5 ml of a 1:1 ratio of compost: perlite and 35 ml of Milli-Q water (Merck), with the remaining volume filled with a 1:1 ratio of compost ...
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bioRxiv - Biochemistry 2020Quote: Freshly purified hASCT2 nanodiscs were concentrated to ∼1 mg ml−1 using an Amicon Ultra-0.5 mL concentrating device (Merck) with a 100 kDa filter cut-off and then 100 μM inhibitor was added and incubated for 1 h on ice ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used were rabbit anti-CB1R (1:1,000, Immunogenes) and mouse anti-actin (1:50,000, MAB1501, Merck Millipore). Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate ...
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bioRxiv - Plant Biology 2022Quote: ... 250 µl of supernatant was added to 14.75 ml HNO3 (17.5%) containing 1 mg l-1 Yttrium (Merck Millipore) as an internal standard ...
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bioRxiv - Microbiology 2022Quote: ... 108 - 109 plaque-forming units (PFU) mL-1 in tryptone soya broth (TSB, Oxoid) or in quarter-strength (1/4) Ringer’s buffer (Merck) unless stated otherwise ...
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bioRxiv - Cell Biology 2022Quote: MCF10 DCIS.com (DCIS.com) lifeact-RFP cells (Riedl et al., 2008) were cultured in a 1:1 mix of DMEM (Merck) and F12 (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then lysed in PK lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 150 mM NaCl, 1% Igepal-630 (Merck)) supplemented with 50 mM DTT and protease inhibitors (cOmplete™ protease inhibitor cocktail EDTA-free ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-K48-Specific Ubiquitin (05-1307, 1:1000) and anti-K63-Specific Ubiquitin (05-1313, 1:1000) from Merck-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... Same volumes of the solvent phase were evaporated under nitrogen and lipids were resuspended in 100 µl of chloroform/methanol 1:1 (v:v) (Merck). Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm ...
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bioRxiv - Cell Biology 2021Quote: ... cultured in SF-900 II SFM at 27°C were transfected at a density of 0.4 × 106 ml-1 with 1 μg bacmid DNA using Excort IV Transfection Reagent (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and incubated overnight in the same solution with the primary antibody to CB1R (1:1,000, rabbit, Immunogenes) and neuronal nuclei (NeuN) (1:1,000, mouse, MAB377, Merck Millipore), at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight incubation at 4°C with primary antibodies (Anti-ASL, Abcam ab97370, 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233 ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mm NaF, 1 mm DTT, 0.1 mm Na3VO4, 1% Triton X-100, 0.1% SDS, and protease inhibitors; Merck, Millipore) and homogenized by sonification ...
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bioRxiv - Microbiology 2022Quote: ... To each tube 1 μL of Tris((1-benzyl-4-triazolyl)methyl)amine (TBTA) solution (2.5 mM in DMSO; Merck), 10 μL of Tetrakis(acetonitrile)copper(I ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Microbiology 2023Quote: ... 108 – 109 PFU (plaque-forming units) mL-1) in TSB (tryptone soya broth, Oxoid) or quarter-strength (1/4) Ringer’s buffer (Merck)) was used in this study ...
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bioRxiv - Microbiology 2023Quote: ... the lipids were resuspended in methanol:chloroform (1:1) and subjected to thin layer chromatography (TLC) using silica gel 60 plates (Merck). Total lipids were separated using chloroform:methanol:water (65:25:4 ...
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bioRxiv - Genomics 2023Quote: ... Fungal biomass was then digested by adding 100 µL of 1 mg mL-1 Chitinase from Streptomyces griseus (Merck) and 200 µL of 50 mM EDTA and incubating at 37 °C for 3 hours ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed twice with PBS and then cultured in a starvation medium composed of DMEM:F12 (1:1) supplemented with BSA (0.3% Sigma-Aldrich/Merck), L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... containing solid (7 g L−1 agar) full-strength Hoagland (2.75 mL well−1; pH adjusted to 6.5) (Sigma-Aldrich, Merck), sealed with parafilm and placed under the growth chamber ...
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bioRxiv - Biophysics 2024Quote: ... Total DNA was extracted by adding 1 mL of a 25:24:1 phenol:chloroform:isoamyl alcohol mixture (Merck, Darmstadt, Germany) along with 1 g of acid washed glass beads (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: A digestion buffer comprising of 0.05 mg/mL activated DNAse I (DNase I with HBSS in a 1:1 ratio, Merck), 10 mg/mL Collagenase (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... pLKO.1-puro-shRNACDK12 (TRCN0000196423 and TRCN0000368335) and pLKO.1-puro-shRNABRCA1 (TRCN0000244984 and TRCN0000244987) were purchased from Merck.
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit (Merck) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The DNA was extracted using a DNA purification kit (Merck Millipore, Darmstadt, Germany). Anti-RNA polymerase II antibody and normal mouse IgG were used as specific antibodies and the negative control ...