Labshake search
Citations for Merck :
2351 - 2400 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... anti-mouse HRP (Merck #A9044; 1:10,000)) for 2 hrs at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (P/S, Merck P4333) and 1x amphotericin B on ice ...
-
bioRxiv - Neuroscience 2024Quote: ... mouse anti-actin (Merck chemicals, 1:1000), rabbit anti-HA (Abcam ...
-
bioRxiv - Neuroscience 2024Quote: ... and B-actin (Merck, A5316, 1:5000) overnight at 4 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1 µg/ml ionomycin (407952; Merck) in the presence of GolgiPlug (555029 ...
-
bioRxiv - Plant Biology 2024Quote: ... Grade 1 (WHA1001325 Sigma-Aldrich, Merck, Germany) to retain the mycelia ...
-
bioRxiv - Plant Biology 2024Quote: ... 1 mg/mL cytochrome c (Merck C7752)] in complex IV buffer) ...
-
bioRxiv - Cell Biology 2024Quote: ... anti-FAM21C (1:1,000 IF, Merck; ABT79), anti-HA (1:1000 WB ...
-
bioRxiv - Neuroscience 2024Quote: ... Rabbit a-Atg8a (1:500, Merck ABC974), mouse anti-Fasciclin II (1D4 Developmental Studies Hybridoma Bank ...
-
bioRxiv - Neuroscience 2024Quote: ... ezrin (1:1000; SAB4200806, Merck, Darmstadt, Germany). Protein bands were detected with host-specific secondary antibodies and developed with a chemiluminescent substrate (GE Healthcare Amersham ...
-
bioRxiv - Neuroscience 2024Quote: ... PFN1 (1:1000; P7624, Merck, Darmstadt, Germany), ezrin (1:1000 ...
-
bioRxiv - Biophysics 2021Quote: ... pH 7.5 and 150 mM NaCl) and concentration processes performed using a dialysis Amicon Ultra 10 K (Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Evolutionary Biology 2019Quote: ... ∼105 log-phase cells from 200 ml mass culture were concentrated on hydrophilic nylon membranes (Nylon-Netzfilter, 10 µm pore size, 47 mm, Merck KGaA), rinsed in mineral water (Volvic® ...
-
bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
-
bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
-
bioRxiv - Molecular Biology 2020Quote: ... flow-through from the 100kDa ultrafiltration step was combined with the non-vesicular fractions from the SEC fractionation and thereafter concentrated on the 10-kDa molecular weight cut-off (MWCO) Amicon spin filters (Merck Millipore), the latter of which was also used to concentrate the vesicular fractions ...
-
bioRxiv - Cell Biology 2022Quote: ... Growth medium was then removed from apical side and on the basolateral side it was replaced by ALI differentiation medium +/- 10 ng/ml IL-13 (IL012; Merck Millipore). Once the ALI interface was established medium was exchanged every second day till day 25-28 on ALI ...
-
bioRxiv - Immunology 2021Quote: ... Color was developed in the dark for 5-10 min before adding 50 µL of 2 M H2SO4 stop solution (Merck, Germany). Optical density was read at wavelength 450 nm using the microplate reader (SpectraMax ID3 ...
-
bioRxiv - Immunology 2019Quote: ... Animals in the 10-day survival experiment were given 500 μl of the antibiotic Imipenem (0.5 μg/kg, Merck, Kenilworth, NJ) and 500 μl of normal saline subcutaneously at the time of laparotomy ...
-
bioRxiv - Microbiology 2019Quote: ... the cell suspension was washed with the same buffer at ∼ 5000 × g for 10 min at room temperature and the pellet was subjected for dehydration with serial dilutions of ethanol (Merck Millipore) (20% ...
-
bioRxiv - Microbiology 2019Quote: ... We dissolved dried lipid extracts in 40 μL of 1:1 methanol:CHCl3 and used 5-10 μL of sample for thin layer chromatography (TLC) on TLC silica gel 60 plates (Merck, Germany) with a mixture of 65:25:10 CHCl3:CH3OH:CH3COOH for the mobile phase ...
-
bioRxiv - Microbiology 2019Quote: ... 2 cm from the base edge of the TLC plates (pre-coated with silica gel TLC plates (20 × 10 cm; Silicagel 60; 0.20 mm layer, Merck, Darmstadt, Germany) in 2 mm bands with an application speed of 250 nl/s ...
-
bioRxiv - Cancer Biology 2020Quote: ... 4°C with a SW28 rotor in a Beckman XL-70 centrifuge or concentrated using an Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore). Pellets were resuspended in 500 µL PBS ...
-
bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
-
bioRxiv - Biochemistry 2019Quote: ... An aliquot of 10 μL of the reaction mixtures were spotted on HPTLC (High Performance TLC) Silica Gel 60 Glass Plates (Merck KGaA), and the plates were developed with a phenol:n-butanol:formate:water (12:3:2:3 v/v ...
-
bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
-
bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
-
bioRxiv - Genetics 2021Quote: ... 10 μg of chromatin was immunoprecipitated with the one of the following antibodies: 5 μg CTCF antibody (07-729, Merck-Millipore), 5 μg RAD21 antibody (Abcam ...
-
bioRxiv - Biochemistry 2020Quote: ... Solubilised mitochondria were then clarified by centrifugation at 16,000 g for 10 min and incubated with pre-washed anti-Flag® M2 Affinity Gel (Merck; A2220) at 4 °C for 2 hours ...
-
bioRxiv - Neuroscience 2020Quote: Samples boiled in 6x Laemmli buffer were subjected to standard SDS-PAGE (10%) prior to being electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Ponceau S staining (Merck ...
-
bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
-
bioRxiv - Plant Biology 2021Quote: ... and added with 10 μl of the appropriate magnetic beads (anti-MyC beads, Thermo Fisher or anti-FLAG beads, Merck Millipore) and incubated for 1 hr at 4 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... The enzymatic digestion was stopped by adding a plating medium (Dulbecco’s modified Eagle medium, D5796, Merck, containing 10% fetal bovine serum). Tissues were dissociated by pipetting ...
-
bioRxiv - Systems Biology 2021Quote: ... Excess FSBA reagent was removed by ultracentrifugation with 15 mL 10 K MWCO Amicon® Ultra-4 centrifugal filter units (Merck) at 3,500 × g at RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... and EV-containing fractions were pooled and concentrated with a 10 kDa molecular weight cut-off Amicon Ultra-4 centrifugal filter unit (Merck Millipore).
-
bioRxiv - Cell Biology 2020Quote: ... with 4.5 g/l (high) glucose and L-glutamine supplemented with 10 % fetal bovine serum (volume fraction, Sigma Aldrich/Merck KGaA) without antibiotics at 37 °C and 5 % CO2 in a humidified atmosphere and regularly confirmed to be mycoplasma negative ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
-
bioRxiv - Cell Biology 2021Quote: ... rinsed four times with MTSB and treated for one hour at RT with 10% dimethylsulfoxide + 3% Igepal CA-630 (Merck # I3021) dissolved in MTSB ...
-
bioRxiv - Immunology 2022Quote: ... with a SuperdexTM 200 Increase 10/300 GL (Cytiva) prior to up-concentration using Amicon® Ultra-0.5 Centrifugal Filter Units (Merck Millipore). The protein eluted as a dimer.
-
bioRxiv - Microbiology 2022Quote: ... dialyzed in buffer C and then concentrated in a centrifugal concentrator with a 10-kDa molecular weight cutoff membrane (Merck Millipore).
-
bioRxiv - Microbiology 2022Quote: ... cultures were maintained at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol ...
-
bioRxiv - Immunology 2022Quote: ... Inhibition of respiratory burst was performed by adding 50 µg/mL of catalase to digest H2O2 and 10 µM of Diphenyleneiodonium chloride (DPI) to inhibit NADPH oxidase (Sigma-Aldrich, Merck) during the overnight coculture ...
-
bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
-
bioRxiv - Cell Biology 2019Quote: ... Afterwards the preparation was washed three times with PBS for 10 min and mounted with FluorSave™ Reagent (Merck, Darmstadt, Germany). Confocal images were collected using the Leica TCS SP8 confocal microscope with HC PL APO CS2 20x/0.75 NA IMM ...
-
bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... After incubation the mixtures were buffer exchanged in centrifugal filter units (Amicon Ultra, 0.5 ml, 10 K, Merck Millipore, Darmstadt, Germany) washed three times with 50 µl of sodium phosphate buffer (20 mM Na2HPO4 ...
-
bioRxiv - Immunology 2020Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 minutes to remove dead cells and concentrated using 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... protein lysate (30 μg) was loaded onto and run through a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck, IPVH00010). Membranes were blocked with 5% (w/v ...