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Citations for Merck :
2601 - 2650 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 100 mM NaCl) and concentrated down to 500 mL using an Amicon® Ultra-15 10 K Centrifuge Filter (Merck Millipore).
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bioRxiv - Microbiology 2022Quote: ... concentrated and buffer exchanged to PBS containing 0.023% DDM using a 10 kDa molecular weight cut-off centrifugal filter unit (Amicon Ultra-15, Ultracel 10K, Merck KGaA, Darmstadt, Germany) to 5 mg/ml as determined by the Bradford protein assay.
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bioRxiv - Cancer Biology 2024Quote: ... Two sets of four EV-rich fractions (7–12 and 16-22) were pooled and concentrated using Amicon Ultra-4 10 kDa centrifugal filter device (Merck Millipore, USA). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
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bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: Medium conditioned by MDA-MB-468 cells was loaded into size exclusion columns (Amicon® Ultra-15 Ultracel-10 Centrifugal Filter Unit, Merck, #UFC901024) centrifuged at 4,000g for 30 min and the fractions stored at -20⁰C until further analysis ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... samples of 8 μM aSyn in the aggregation buffer were incubated at 37 °C under quiescent conditions and over 20 h with and without a cylindrical 10 mm x 3mm Teflon stirring bar (Merck, ref. Z328650) or 0.5 μM oTTR ...
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bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... proteins were ultracentrifuged at 13000 rpm for 10 min at 4°C and concentrated to 1.5 ml using centrifugal filters (Merck, Amicon Ultra-4). They were then exchanged into buffer #5 overnight at 4°C and aliquoted and stored at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Biochemistry 2023Quote: ... samples were concentrated to a volume of approximately 2 mL using Amicon® Ultra centrifugal filters with a NMWC of 10 kDa (cat# UFC801024, Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Biophysics 2023Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 μl M2B-taxol (M2B supplemented with 10 μM taxol (T7402, Merck, Darmstadt, Germany).
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Flies were raised at 25℃ and collected on the day of eclosion and kept in the dark for 7-10 days on the fly media containing 0.4 mM all trans-Retinal (Merck, St. Louis, MO). The experiments were performed with the “8-well” chamber (16 mm diameter x 10 mm height ...
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bioRxiv - Biochemistry 2023Quote: PrPC was filtered through a 100 kD membrane filter in 10 mM Bis Tris pH 6.5 (Amicon ULTRA 0.5 mL 100K 96PK, UFC510096, Merck Life Science UK Ltd) to remove aggregates and then diluted into reaction buffer (50 mM Na-phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation on ice for 10 min and loaded on SDS-PAGE gel and wet transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Thereafter ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein containing fractions were pooled and buffer exchanged using SnakeSkin dialysis tubing (10 MWCO, Pierce Cat#68100) followed by further dialysis and concentration using Amicon Ultra-4 10kDa centrifugal units (Merck, Cat# UFC801096), as described previously (44).
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bioRxiv - Molecular Biology 2023Quote: ... filtered through a 0.45 µm filter and supplemented with 10 mM HEPES buffer (BI, 03-025-1B) and 12.5 µg/ml polybrene (Merck, TR-1003-G). MLV-based GFP-reporter (pNCA-GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Cancer Biology 2024Quote: ... a concentrated lipid mixture stock solution of fatty acids conjugated to fatty acid-free BSA (10% solution in PBS) (Merck cat# A1595) was created as previously described71 ...
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bioRxiv - Microbiology 2024Quote: ... 20 °C) from NO2- replete active cultures using Amicon Ultra-15 10 kDa cut-off centrifugal filter units (Merck Millipore, Darmstadt, Germany). Concentrated cells were washed with and resuspended in substrate-free medium (pH 6 ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 4.5–6 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). The second transfection was performed 3 days after the first transfection and the cells were harvested after an additional 5 days ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 3–5 μg of plasmid and dsRNA were transfected into BmN4 cells (∼2 × 106 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche). Transfection was repeated 2 days and 5 days after the first transfection and the cells were harvested after an additional 4 days ...
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bioRxiv - Microbiology 2024Quote: ... the fractions containing each component of the assembled PhoP-TAC were concentrated using Amicon Ultra centrifugal filters (10 kDa MWCO, Merck Millipore, Inc.). In addition ...
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bioRxiv - Cancer Biology 2024Quote: ... 09499-14) were subjected to SDS-PAGE on a 10% polyacrylamide gel and subsequently transferred to Immobilon-P PVDF-membrane (Merck Millipore, IPVH85R). The membrane was blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-shGAL-9 (MISSION® shRNA library, Merck), psPAX2 and pMD2.G (packaging vectors were a kind gift from Dr ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-SOX2 1:500 (AB5603 Merck Millipore), mouse anti-Tuj1 1:500 (801202 Biolegend) ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-active-ß-catenin (1:300; Merck Millipore), anti-MHC (#M4276 1:200 Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-Ect2 (07-1364; Merck). Secondary antibodies used were 1:500 Alexa flour 488 goat anti-mouse (A11001 ...
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bioRxiv - Cell Biology 2020Quote: For Western blot: α-Puromycin (Merck 1:12,500), α-mouse antibody conjugated with HRP (Bethyl) ...
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bioRxiv - Genetics 2021Quote: ... mouse anti-NR1 (1:1000, Merck Millipore, UK), rabbit anti-PSD95 (1:2000 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Actin(20-33) (1:1000, Merck #A5060), anti-mNeonGreen [32F6] ...
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bioRxiv - Neuroscience 2021Quote: ... peroxidase-conjugated (1:4000, Merck EMD Millipore, Ap132P). Immunoreactivity was revealed using the Clarity™ ECL Western Blotting Substrate kit (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-SOX2 1:500 (AB5603 Merck Millipore), mouse anti-TUJ1 1:500 (801202 Biolegend) ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Histone at 1:100 (Merck #MAB3422), mouse anti-γ-tubulin at 1:250 (Sigma-Aldrich # T5326) ...
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bioRxiv - Developmental Biology 2020Quote: ... or an anti-GAPDH antibody (1:104; Merck) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... 1% penicillin-streptomycin and 0.00035% 2-mercaptoethanol (Merck)) to an orbital shaker ...
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bioRxiv - Systems Biology 2020Quote: ... 1% benzonase (25 U/μL; Merck,; Kenilworth, NJ), 1% sodium dodecyl sulfate (SDS ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-βIII-Tubulin (Merck, #MAB1637, 1:200) in blocking solution overnight at 4C° ...
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bioRxiv - Cell Biology 2022Quote: ... 1:100 (Merck Life Science, S.L., Cat# MAB2058), mouse Anti-Lamin A + Lamin C antibody [131C3] 1:200 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... Arp3 (rabbit anti-Arp3, 07272 Merck; 1:1000), N-WASP (mouse anti-N WASP ...
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bioRxiv - Neuroscience 2021Quote: ... PSD95 (mouse anti-PSD95, MAB1596 Merck; 1:1000), SAP102 (mouse anti-SAP102 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of 1 M DTT (Merck, D9779) was added and the sample was heated for 10 min at 70°C ...
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bioRxiv - Cell Biology 2022Quote: ... 1% Pen/Strep (Merck Life science UK limited). Cells were cultivated till 50-60% then seeded into new t25 flasks until 20-30% or 50-60% confluency was reached for subsequent low confluency or high confluency total RNA extractions respectively.
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bioRxiv - Molecular Biology 2019Quote: ... or anti-RAD51 (1/500, PC130, Merck Millipore) antibody for 2 h at RT or overnight at 4°C ...