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Citations for Merck :
201 - 250 of 2295 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and for miniTurbo–TAF protein expression using a rat anti-HA-HRP antibody (3F10, Merck, 12013819001). The protein concentration was determined with Protein Assay Dye Reagent Concentrate (Biorad ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated in 50 μL of DMEM containing 200 μM D-biotin (Merck), 20 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... blotted onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) via semi-dry blotting system and protein signal analyzed by using enhanced chemiluminescence to detect bound antibodies (WesternBrightTM ECL ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to PVDF Immobilon-P transfer membrane (Merck-Millipore, Darmstadt, Germany) as previously described (32) ...
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bioRxiv - Cell Biology 2022Quote: SDS Polyacrylamide gels were transferred onto Immobilon-P Transfer Membrane® (MERCK) using Transfer-Blot® Semi Dry apparatus (BIO-RAD) ...
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bioRxiv - Cancer Biology 2023Quote: ... and transferred to Immobilon-P Transfer Membrane (IPVH00005, Merck Millipore, Burlington, MA). Blots were probed overnight at 4 °C with primary antibodies detailed in Supplementary Table S8 ...
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bioRxiv - Cell Biology 2022Quote: ... and 44µl 90mM p- coumaric acid (Merck, #C9008-5G, solved in DMSO) dissolved in 1ml 1M Tris pH 8.5 ...
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bioRxiv - Genomics 2022Quote: ... 1.2 mL Phenol Red solution) containing 10 mg of collagenase P (Merck) for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P, Merck Millipore, Burlington, MA), and probed with specific antibodies ...
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bioRxiv - Microbiology 2024Quote: ... PNPG (p-nitrophenyl-alpha-D-glucopyranoside) LOT: 3533550 was obtained from Merck Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Cell Biology 2021Quote: ... then induced to differentiate in culture medium supplemented with 8 µg/mL biotin (Merck, B4639), 8 µg/mL D-pantothenic acid (Merck ...
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bioRxiv - Microbiology 2023Quote: ... was added to the fox and wolf samples and antiferret IgG biotin (Merck, Darmstadt, Germany) was added to the mustelid samples ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in 250 μL 1X PBS solution containing 0.01 mg/mL FSL-biotin (Merck). The cells were incubated at 37°C for 45 min ...
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bioRxiv - Genomics 2020Quote: ... 0.1% (w/v) SDS and wet-transferred to Immobulin-P (Merck Millipore, IPVH00010) or Immobulin-FL membranes (Merck Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... a calibration curve was plotted with p-NP as standard (Merck, Darmstadt, Germany). Endo-xylanase activity was determined using beechwod xylan (1% w/v ...
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bioRxiv - Microbiology 2022Quote: ... All tissue culture media was supplemented with 1% penicillin/streptomycin (P/S) (Merck Life Science UK Ltd ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 % penicillin/streptomycin (P/S) solution (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) at 5 % (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... antibody complexes were purified by incubation of lysate with Protein A Sepharose® beads (Merck, GE17-5138-01) for 3h rotating at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Rabies injected brains were immunohistochemically stained using antibodies against the 2A linker protein (Merck/ Millipore, ABS31, 1:2000) found in tTA expressing cells ...
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bioRxiv - Cell Biology 2024Quote: ... RSV F-protein was detected using a mouse anti-RSV antibody used 1:5000 in casein (MAB8262, Merck), which was incubated at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... then transferred to Immobilon®-P PVDF Membrane (Merck Millipore Ltd., Catalog N° IPVH00010) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Then samples were transferred to PVDF membrane (Immobilon-P, 0.45 μm Merck Millipore Ltd.), which was activated prior to transferring in 100% methanol following a wash in ddH2O ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8.3) and then transferred to Immobilin-P polyvinylidene difluoride (PVDF) transfer membranes (Merck) at 70 V for 75 min in transfer buffer (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were subject to SDS-PAGE and transferred onto Immobilon-P PVDF-membrane (Merck). Membranes were blocked in 5% milk in PBST or in 5 % BSA with TBST for 30mins ...
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bioRxiv - Biophysics 2022Quote: ... and 1% penicillin/streptomycin (P/S) Solution (P0781, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), in an environment with 5% CO2 (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were covered with 1ml lysis buffer [0.5% Nonidet P-40 Substitute (Merck), protease inhibitor cocktail (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... After separation samples were transferred from gel to Immobilon P PVDF membrane (Merck, Germany) by semi-dry transfer ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a wet transfer onto Immobilon-P Polyvinylidene Fluoride (PVDF) membrane (Merck Millipore) using the Mini Trans-Blot Cell system (BioRad).
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bioRxiv - Bioengineering 2024Quote: ... which uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate with ALP enzyme (Merck) as a standard ...
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bioRxiv - Microbiology 2023Quote: Purified proteins and native protein standard (Supelco-Merck) were loaded in 4-15% gradient mini protean precast native gel (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of newly synthesized proteins was carried out by an anti-puromycin antibody (clone 12D10, mouse-monoclonal, MABE343; Merck), an anti-HuD antibody (ab96474 ...
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bioRxiv - Cell Biology 2021Quote: ... Virotrap particles containing protein complexes were purified in a single step using biotinylated anti-FLAG BioM2 antibody (#F9291, Merck) and Dynabeads MyOne Streptavidin T1 Beads (#65601 ...
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bioRxiv - Microbiology 2022Quote: ... The sections were then incubated overnight in PBS with 0.2% BSA and 0.05% Tween-20 with primary antibodies directed against SARS-CoV-2 Nucleocapsid Protein (1:500; mouse monoclonal, # ZMS1075, Merck); Iba1 (1 ...
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bioRxiv - Molecular Biology 2024Quote: ... immunoprecipitations performed by incubating with 8 μl Protein G Dynabeads (Novex) and 12 μl anti-FLAG antibody (F3165, Merck) per sample for 90 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... for normalization of protein loading following which western blotting was performed using an anti-HA antibody (H9658 Merck, UK). HA tagged protein bands were visualised using the LI-COR Odyssey Clx system with LI-COR secondary antibody (IRDye 800 CW anti-mouse ...
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bioRxiv - Biochemistry 2021Quote: ... the actin was mixed with a fivefold molar excess of NHS-XX-Biotin (Merck KGaA, Darmstadt, Germany) and incubated for 15 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... medium was changed and replaced with standard growth medium complemented with 200 µM biotin (Merck, B4639-1G) for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... unreacted biotin-pentylamine was removed by ultrafiltration with a 3 or 10 kDa MWCO membrane (Merck Millipore). The recovered upper residual liquid (∼100 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... which can be biotinylated in vitro by incubation with bacterial BirA biotin ligase (Merck, cat. No. SRP0717). Biotinylation enabled us to label Pol II via Streptavidin-conjugated Alexa FluorTM 647 (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: The toxins were biotinylated using a 10:1 molar ratio of biotinylation reagent (Innolink Biotin 354S, Merck) to toxin as recommended by the manufacturer ...
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bioRxiv - Biophysics 2023Quote: ... the actin was mixed with a 5-fold molar excess of NHS-XX-Biotin (Merck KGaA, Germany) and incubated in the dark for 15 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... for 24 h and then used for a time-course of release by adding biotin (#4698, Merck) at a final concentration of 40 μM as previously described (Capitani et al. ...
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bioRxiv - Immunology 2024Quote: ... we added 50µL Biotin anti-mouse IgG (4µg/mL) (Sigma-Aldrich B7022; Merck Life Science; Hoeilaart, Belgium) to each well and incubated for 40 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... prior to transfer to a PDVF membrane (Immobilon-P Membrane, Merck Millipore Ltd., Burlington, MA). The membrane was then incubated in a 5% milk tris-buffered saline with 0.1% tween-20 (TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% P/S and 6 ng/mL human insulin (I9278-5ML, Merck Life Science bv). The cell culture was checked for mycoplasma contamination with the MycoAlert® Mycoplasma Detection Kit (LT07-118 ...
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bioRxiv - Genetics 2021Quote: ... Prior to EMSAs the in vitro translations of Rbpj (WT) and mutant proteins were monitored by western blotting using an anti-Flag antibody (M5, Merck).
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...