Labshake search
Citations for Merck :
51 - 100 of 2295 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... 0.84 uM biotin (Merck), 1.7 µM insulin ...
-
Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Proteins of interest were detected using the following primary antibodies ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to a PVDF membrane (0.45µm Immobilon®-P, Merck Millipore Ltd., VIC, Australia) at 100 V in chilled transfer buffer containing 25 mM Tris ...
-
bioRxiv - Plant Biology 2023Quote: ... and proteins were blotted onto a 0.45 µm PDVF Immobilon-P membrane (Merck Millipore, ref. IPVH00010) after electrophoresis ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto methanol pre-activated polyvinylidene fluoride (PVDF) membranes (IPFL00010, Immobilon-P; Merck-Millipore). Membranes transferred from TGX Stain-Free™ gels were imaged and quantified for posterior normalization with a ChemiDoc XRS+ (Bio-Rad ...
-
bioRxiv - Plant Biology 2022Quote: ... The separated proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA) and analyzed with the following antibodies ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred on a PolyVinyliDene Fluoride (PVDF) membrane (Immobilon-P, Merck Millipore, Burlington, USA) using liquid transfer with 20 % methanol at 100V ...
-
bioRxiv - Plant Biology 2022Quote: The target subunits were purified and desalted using C18-ZipTips (Merck, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were transferred on an Immobilon™-P Membran (PVDF, 0.45 µm, Art. No. IPVH00010, Merck Millipore) for 16 h at 15 V using a Mini Trans-Blot® Cell (Art ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins in each lysate were resolved by SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck). The blots were incubated with the following primary antibodies ...
-
bioRxiv - Systems Biology 2023Quote: ... The separated proteins were transferred in a semi-dry system onto PVDF membranes (Immobilon P, Merck Millipore) using transfer buffer (192 mM glycine ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by SDS-PAGE system and transferred to Immobilon-P membrane (Merck KGaA, Darmstadt, Germany). Immunoreactions were detected according to standard methods described previously [Yoshitake et al ...
-
Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
-
bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a Immobilion®-P Transfer Membrane (0.45 µm pore size, Cat#: IPVH00010, Merck) and blocked for 1h at RT in 1xTris Buffered Saline Tween (TBST ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE (7.5%–10% PAG) and transferred onto Immobilon-P membranes (Merck Millipore). Membranes were immunoblotted with the respective antibodies as follows ...
-
bioRxiv - Genetics 2024Quote: ... proteins were transferred on an Immobilon™-P Membran (PVDF, 0.45 μm, Art. No. IPVH00010, Merck Millipore) for 16 h at 15 V using a Mini Trans-Blot® Cell (Art ...
-
bioRxiv - Systems Biology 2020Quote: ... biotin (13 μM, Merck, B4639) was added for protein biotinylation ...
-
bioRxiv - Physiology 2024Quote: ... and biotin-conjugated IB4 (Merck) followed by Alexa Fluor 488-conjugated streptavidin (Invitrogen) ...
-
bioRxiv - Neuroscience 2022Quote: ... directed against N-terminal epitopes and rabbit p-Ser491 Dab1 antibody (AB9642) were from Merck Chemicals (Darmstadt ...
-
bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
-
bioRxiv - Physiology 2024Quote: ... B-subunit (CTB, 1/1000, goat polyclonal, Calbiochem #227040, Millipore-Merck, Guyancourt, France). The secondary antibody anti-goat was linked to the fluorochromes Alexa Fluor 594 (1/2000 ...
-
bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore ...
-
bioRxiv - Immunology 2020Quote: Antibody to Ubiquitinated proteins (FK2) from Merck-Millipore was used for the immunoprecipitation assay.
-
bioRxiv - Immunology 2021Quote: ... Proteins were electrophoretically separated and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Merck Millipore, Burlington, MA, USA). Blocking treatment was performed using the PVDF Blocking Reagent for Can Get Signal (Toyobo ...
-
bioRxiv - Biochemistry 2020Quote: ... were separated by SDS-PAGE and protein was transferred to MeOH-activated Immobilon®-P membrane (Merck Millipore) using Whatman paper and a transfer buffer of 48 mM Tris ...
-
bioRxiv - Biochemistry 2024Quote: ... Fractions containing Nipah L-P protein were concentrated again using a MWCO 100,000 Amicon Ultra Centrifugal Filter (Merck). The protein with a final concentration of 1.5 uM was freshly used for grids preparation and/or aliquoted and flash-frozen and stored at 80 °C until use ...
-
bioRxiv - Physiology 2024Quote: ... the P38 inhibitor SB203580 and the SFK inhibitor PP2 were all purchased from MERCK (Dorset, United Kingdom) and used at a concentration of 10 μM unless otherwise specified.
-
bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Thirty micrograms of cellular protein were resolved by 10 % SDS PAGE and were transferred to Immobilin P 0.45 μm PVDF membrane (Merck). Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were subjected to SDS-PAGE and proteins were transferred to Polyvinylidene difluoride (PVDF) membranes (Immobilon®-P, Merck). The ATP2 proteins fused to the BAD tag were detected with the Avidin HRP-conjugate probe (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were separated using a 10% SDS-PAGE gel and transferred onto a PVDF Immobilon-P membrane (Merck Millipore). The membrane was blocked with 5% w/v milk in TBST (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred from the gel to a PVDF membrane (Immonilon-P transfer membrane, Merck, Darmstadt, Germany) using the wet transfer method ...
-
bioRxiv - Bioengineering 2024Quote: ... Forty micrograms of cellular protein were resolved by 10% SDS-PAGE and transferred to an Immobilon-P 0.45 μm PVDF membrane (Merck). Bound IgG was visualized using horseradish peroxidase-conjugated secondary antibodies and Clarity™ Western ECL Substrate (Cat no ...
-
bioRxiv - Neuroscience 2024Quote: ... the elution of the biotinylated proteins was carried out boiling the beads in 25 μl of 3X protein loading buffer supplemented with 20 mM DTT and 2 mM Biotin (Merck, B4501) for 10 min at 95°C in gentle shaking ...
-
bioRxiv - Immunology 2022Quote: ... Immobilon-P® (Merck Millipore). Membranes were incubated with anti-IκBα (product # ...
-
bioRxiv - Biochemistry 2024Quote: ... Substance P (Merck, Darmstadt, Germany), and two further synthetic peptides (KLKESYCQRQGVPMN ...
-
bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
-
bioRxiv - Immunology 2020Quote: ... The proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto an Immobilon-P membrane (IPVH00010; Merck). The membranes were blocked with 10% nonfat milk (190-12865 ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Amershan Hybond P PVDF membrane, Merck). Membranes were blocked in 5% milk in PBS/0.1% Tween-20 ...
-
bioRxiv - Neuroscience 2024Quote: ... and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Membranes were blocked for one hour in I-BlockTM (# T2015 ...
-
bioRxiv - Biophysics 2020Quote: ... was labeled with Biotin-NHS (Merck, 203112). Therefore 100 µg of 1g/l primary antibody were treated according to the labeling protocol above using 250 µg of Biotin-NHS ...
-
bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal anti-biotin (Merck, BN-34), Mouse monoclonal anti-PARP1 (Santa Cruz ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.1 mM azide-PEG3-biotin conjugate (Merck) and 2 mM copper sulfate (Merck ...
-
bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
-
bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
-
bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
-
bioRxiv - Microbiology 2023Quote: ... 20 µg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon®-P PVDF membrane (Merck), followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500 ...
-
bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
-
bioRxiv - Biophysics 2020Quote: ... After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK) followed by a gel filtration step (Superdex-200 10/300 GE Healthcare Life Sciences) ...