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Citations for Merck :
351 - 400 of 2295 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6x Laemmli buffer were subjected to standard SDS-PAGE (10%) prior to being electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Ponceau S staining (Merck ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were loaded on 8% gels and separated by SDS-PAGE followed by the transfer done on to Immobilon-P® (Merck) PVDF membranes at 106 V for 75 minutes.
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bioRxiv - Neuroscience 2022Quote: ... from CNO and saline treated mice were first boiled at 95°C for 5 min and then separated on 10% polyacrylamide gel and transferred on PVDF membranes (Immobilon-P transfer membrane; Merck Millipore). Membranes were then blocked with 5% non-fat powdered milk in TBS + 0.01% Tween-20 (TBS-T ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were electrotransferred during a 30-min interval from the gel onto an Immobilon-P membrane of pore size of 0.45 µm (Merck Millipore, USA) under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Neuroscience 2022Quote: ... and afterwards transferred to a methanol- activated Immobilon-P PVDF (poly-vinylidene difluoride) membrane (catalog#IPCVH00010, Merck Millipore Ltd., Carrigtwohill, Ireland) by tank blotting at 250mA for 90 min in a cold room (4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Biochemistry 2024Quote: ... The activity assay was performed by incubating 0.5 nM PT5 with 100 μM chromogenic substrate N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (100 μM; Merck, Darmstadt, Germany) and two-fold macrocyclic peptide dilutions ranging from 0.0005 to 2000 nM ...
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bioRxiv - Developmental Biology 2024Quote: ... The fins were then washed again with DPBS and then digested for 20 min in Collagenase P solution (1mg/ml, Merck Millipore) in Leibovitz’s L-15 medium ...
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bioRxiv - Biochemistry 2024Quote: ... prepared by partial heparinase I digests of pig mucosal heparin were obtained from Iduron (Manchester, UK) and biotinylated at the reducing end using biotin-XX-hydrazide (Calbiochem, Merck Biosciences Ltd, Nottingham, UK), as described (Delehedde ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
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bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
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bioRxiv - Immunology 2021Quote: ... Cartilage was dissected from the tibial plateau and minced before overnight collagenase digestion in DMEM-F12 supplemented with 1% P/S and 1mg/ml collagenase IA (Sigma, supplied by Merck, Darmstadt, Germany). Synovium was minced before being collagenase digested for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail [5 μl/ml] and phosphatase inhibitor cocktail [10 μl/ml]) and 0.5% Nonidet P-40) (I8896; Sigma-Aldrich/Merck group, USA). After 10 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... 09499-14) were subjected to SDS-PAGE on a 10% polyacrylamide gel and subsequently transferred to Immobilon-P PVDF-membrane (Merck Millipore, IPVH85R). The membrane was blocked with 5% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... 500 ng of BiP were separated via SDS-PAGE on 10 % polyacrylamide gels (homemade) and transferred on Immobilon®-P PVDF membranes (Merck-Millipore) for 1.5 h at 230 mA using a Biometra Fastblot B44 (Analytik Jena ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin pre-clearing was done by incubating with Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) for 4 hrs at 4°C ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and eSpCas9-GFP protein (#ECAS9GFPPR, Merck KGaA) were introduced into MCF7 and T47D cells using Viromer® CRISPR (Lipocalyx GmbH ...
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bioRxiv - Evolutionary Biology 2020Quote: ... protein G-magnetic beads (Merck Millipore, LSKMAGG10) were used instead ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Cell Biology 2021Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Biochemistry 2021Quote: ... MWCO 10 kDA protein concentrator (Merck Millipore) to the final concentration of 9-10 mg/ml.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (Merck). The membranes were blocked with 5% defatted milk before blotted with primary antibodies and secondary antibodies [anti-mouse IgG-HRP (at 1:2,000 dilution) ...
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bioRxiv - Biophysics 2021Quote: ... proteins were transferred to nitrocellulose membranes (Merck). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... separated proteins were transferred to PVDF (Merck) membranes for immunoblotting ...
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bioRxiv - Molecular Biology 2021Quote: ... protein lysates were reduced with dithiothreitol (Merck) and cysteine residues were alkylated with iodoacetamide (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred onto PVDF membranes (Merck) and blocked using 5% non-fat dried milk in PBS-Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... anti-surfactant protein C (SFTPC, AB3786, Merck), homemade anti-α-smooth muscle actin (clone 1A4 ...
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bioRxiv - Cell Biology 2023Quote: ... together with Protein-A Sepharose beads (Merck). Beads were washed 3x in 0.1% (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred on a nitrocellulose (Merck) membrane by wet transfer at 15 V for 6h at 4°C and blocked with 2% skimmed milk at room temperature for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... S-protein agarose (Merck KGaA, Darmstadt, Germany), Dynabeads Protein G (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was measured by the infrared (IR)-based protein quantitation method with a Direct Detect Infrared Spectrometer (Merck). Fifty or thirty-five (depending on the protein detection ...
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bioRxiv - Immunology 2022Quote: ... The protein concentration of ES antigen was estimated using protein estimation kit by Bradford method (Merck GeNei™, Bangalore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: The eluted corona proteins from nanoparticles were first precipitated by using the ProteoExtract® Protein Precipitation Kit (Merck, Germany) as described in the manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibody (Merck guinea pig anti-NeuN 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were mouse anti-EGFR antibody (#GR01; Merck Millipore), goat anti-EEA1 (#sc-6414 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody RNA-DNA hybrid (s9.6) antibody (Merck Millipore, #MABE1095, 3ul) or GFP (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... A total of 50 μg of protein from each sample was transferred to a protein LoBind plate (Merck, NJ, USA). Protein was reduced with tris (2-carboxyethyl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were concentrated with Centricon filters (Merck-Millipore) when needed and/or diluted to a final concentration of 1 mg/mL in PBS.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were detected by using Luminata Crescendo (Merck) and LAS600 (GE Healthcare) ...