Labshake search
Citations for Merck :
301 - 350 of 2295 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
-
bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Cancer Biology 2021Quote: Intracellular protein was extracted from cells using the NucBuster Protein Extraction Kit (Merck) and quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein concentration was estimated using BCA protein assay kit (Merck, 71285-3, Germany).
-
bioRxiv - Neuroscience 2021Quote: ... protein concentration was quantified using a Sigma-Aldrich BCA protein assay kit (Merck). Subsequently ...
-
bioRxiv - Biophysics 2020Quote: ... Fractions containing monomeric I-Ek/MCC(ANP)-biotin complexes were concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK), snap frozen in liquid nitrogen and stored in 1x PBS at −80 °C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by washing with 1XPBS two times for 2’ each and blocked again with Biotin (0.001%, Sigma-Aldrich, Merck, Overijse, Belgium) for 20’ and washed twice for 2’ in 1XPBS each ...
-
bioRxiv - Genomics 2024Quote: ... in modified RPMI with L-glutamine and without biotin and phenol red (US biological life sciences, R9002-01) supplemented with 200 µM Hypoxyxanthine (Merck, H9377) and 0.5% AlbuMAX TM II (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: ... We tested several markers to label myelinated axons and settled on using an antibody against myelin basic protein (MBP; Merck, NE1019-100UL, 1:1000). We used two different antibodies for both PV (Sigma ...
-
bioRxiv - Physiology 2020Quote: ... Total proteins from the supernatant were quantified using a Bradford Protein Assay Kit (Merck) and subjected to electrophoresis on 15% SDS–PAGE gels ...
-
bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were electrophoresed on denaturing SDS-polyacrylamide gels and transferred onto Immobilon-P PVDF membranes (Merck Millipore, USA). The membranes were then blocked using 5% non-fat milk in PBS for 30 minutes followed by overnight incubation at 4°C with primary antibody against protein of interest at the desired dilution in 2% non-fat milk prepared in PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore®; Merck). Details of primary antibodies used are provided in Supplementary Table 2 ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein samples (5 to 25µg/lane) were separated by 12.5% – 15% SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Merck Millipore). Proteins were probed in Can Get Signal Solution1 (Toyobo ...
-
bioRxiv - Cell Biology 2023Quote: ... SDS-protein samples were separated by electrophoresis using a handmade SDS-polyacrylamide gel (7.5 or 9% polyacrylamide) and transferred to a PVDF membrane (Immobilon-P, pore size 0.45 µm; Merck-Millipore). The membrane was probed with primary antibodies listed in Supplemental Table 3.
-
bioRxiv - Cancer Biology 2023Quote: ... 4561086) and transferred at 4°C to either Immobilon-FL or Immunoblon-P (IPVH00010) polyvinylidene fluoride membranes (Merck, IPFL00010) (1.5 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... Proteins were separated on SDS-PAGE gels using Tris-Glycine buffer and were either subjected to Coomassie Blue staining or transferred to an Immobilon-P 0.45 μm PVDF membrane (Cat no. IPVH00010, Merck). The membrane was blocked with 5% skim milk blocking buffer for 1 hour and incubated with a primary antibody overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... A Bio-Rad wet transfer system was used for immunoblotting onto PVDF membranes (Immobilon-P, 0.45-μm pore size, IPVH00010, Merck) before incubation with appropriate primary and secondary antibodies in 5% skimmed milk ...
-
bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
-
bioRxiv - Microbiology 2023Quote: ... protein A sepharose beads (Merck) were incubated with the cleared supernatant for an hour to remove non-specific binding ...
-
bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
-
bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated O/N at 4 °C with the following primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP; Merck, Madrid, Spain; #MAB360; 1:1000) and rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba1 ...
-
bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...
-
bioRxiv - Physiology 2024Quote: Total protein carbonylation was detected by immunoblotting using the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... and were transferred at 100 mA for 70 min onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using semi-dry blotting system ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
-
bioRxiv - Cell Biology 2021Quote: The samples were loaded onto a 5–20% gradient SDS-PAGE gel (Wako, Osaka, Japan) and transferred to an Immobilon-P Transfer Membrane (Merck). Antibodies were diluted with Signal Enhancer HIKARI for Western Blotting and ELISA (Nacalai Tesque) ...
-
bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
-
bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
-
bioRxiv - Cell Biology 2020Quote: ... in ice-cold serum-free RPMI media (R5886, Merck, with 100 U/μg/ml penicillin/streptomycin [P/S, P0781, Merck] and 2 mM L-glutamine [L-glut ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated in a buffer containing 20 mM HEPES-KOH (pH 7.5) and 0.2% Noidet P-40 using a dialysis spin column (Amicon Ultra-10; Merck, Darmstadt, Germany). The concentrations of purified fluorescent proteins were measured using fluorescence correlation spectroscopy (FCS) ...
-
bioRxiv - Physiology 2024Quote: ... the pancreas was perfused via the pancreatic duct using an ice-cold collagenase solution (Collagenase P, 11213865001, Merck/Sigma Aldrich) with a concentration of 0.63 mg/ml ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then washed with three rounds of PBS before incubation in 200 µl p-Nitrophenol phosphate substrate solution for ELISA (Merck) for 10 min ...
-
bioRxiv - Microbiology 2024Quote: ... Wells were again washed three times with PBS and bound alkaline phosphatase quantified using a p-Nitrophenol phosphate substrate solution for ELISA (Merck) and A405 readings.
-
bioRxiv - Biochemistry 2024Quote: ... For the determination of the Ki inhibitory constant of macrocyclic peptide inhibitor MP5.4.3 against PT5 we used chromogenic substrate N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (100 μM; Merck, Darmstadt, Germany) at final concentration of 100 μM ...
-
bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
-
A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
-
bioRxiv - Immunology 2024Quote: ... Proteins were alkylated with iodoacetamide (Merck) and sequentially digested on the filter with LysC (Wako ...
-
bioRxiv - Microbiology 2024Quote: ... Protein A-Sepharose beads from Merck-Cytiva (USA) ...
-
bioRxiv - Biophysics 2024Quote: ... Primary anti-KI67 antibody (Merck, rabbit polyclonal antibody) was diluted 1:300 in antibody buffer (0.2 % Tween 20 ...
-
bioRxiv - Neuroscience 2021Quote: ... The samples were separated by SDS–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane (Hybond-P PVDF membranes, Merck, Germany) by a wet-transfer apparatus (Vep-3 ...