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Citations for Merck :
2401 - 2450 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and the fractions with the target protein were mixed and concentrated using Amicon Ultra-15 Centrifugal filters (Merck Millipore) with a 3 kDa membrane cutoff (Figure 10).
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bioRxiv - Immunology 2021Quote: ... the protein content of the supernatants was concentrated 10x using centrifugal filters with 10 kDa cutoff (#UFC501096, Amicon, Merck). The concentrated samples were boiled in a reducing sample buffer and separated in 4-12% polyacrylamide gel (#NW04122BOX ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein pellets were resuspended in 1x S-Trap lysis buffer (5% SDS, 50 mM TEAB from Merck, pH 8.5). 50 µg of the sample protein was digested using the S-trap micro spin column digestion protocol from ProtiFi LLC ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... with the resulting protein pellet resuspended in Tris buffer and sterilized using 0.45 μm and 0.2 μm syringe filters (Merck, US).
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted by RIPA lysis buffer (EcoTech Biotechnology, RIPA-100) containing cOmplete™ protease inhibitor cocktail (Merck, 11697498001), PMSF (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... Expression of mScarletI-PXVam7 from the pCUP1 promoter or of down-regulated proteins from the weak pCUP1(dim) promoter was induced by supplementation of 10μM (unless noted otherwise) copper sulfate (Merck) to the growth medium ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Citrullination of proteins was induced via activation of PADI4 by the addition of calcium ionophore Af23187 (cat. C7522, Merck) to a final concentration of 4 µM ...
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bioRxiv - Biophysics 2023Quote: ... Protein extracts (50 μg/lane) were resolved in 15% SDS-PAGE followed by immunoblotting using anti-GFP (AB3080P, Merck), anti-PrPC monoclonal antibody (anti-CD320 clone 6D11 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Physiology 2023Quote: ... For citrate synthase activity, 15-30 µg protein was added to the reaction buffer (20 mM Tris-HCl, pH 8.0 (Merck), 0.42 mM acetyl-coenzyme A (Sigma) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein content of supernatant samples were normalized and analyzed using SDS-PAGE (mPAGE Bis-Tris 8%, Merck, Darmstadt, Germany) revealing specific protein bands in the supernatant while comparing wildtype and the T2SS mutant (xpsxcs ...
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bioRxiv - Plant Biology 2023Quote: ... Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck), flash-frozen in liquid nitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... The resin was pelleted as above and washed twice with 0.8 mL plant extraction buffer (see plant protein extraction section above) supplemented with 10 μM MG132 (Merck). Cold plant extract was either added directly to the resin (Supplementary Dataset S1 ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated using a centrifugal filter unit (Amicon Ultra, 10 or 30 kDa cut-off, Merck), and the solvent was exchanged with 50 mM potassium phosphate buffer (pH 7.0 ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected as control and the beads were thoroughly washed 3 x with TBS/Tween 0.2% after which bound proteins were eluted by adding hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck) and boiling for 5 min at 99 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... protein concentration was determined by IR spectrometry measuring amide bond absorbance using a Direct Detect IR Spectrometer (Merck KGaA). If protein concentration (of the undiluted lysate ...
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bioRxiv - Cancer Biology 2024Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... immunoprecipitations performed by incubating with 8 μl Protein G Dynabeads (Novex) and 12 μl anti-FLAG antibody (F3165, Merck) per sample for 90 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... for normalization of protein loading following which western blotting was performed using an anti-HA antibody (H9658 Merck, UK). HA tagged protein bands were visualised using the LI-COR Odyssey Clx system with LI-COR secondary antibody (IRDye 800 CW anti-mouse ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from cells or tissues by RIPA buffer supplemented with a protease inhibitor cocktail (Merck, #11836170001) and phenylmethanesulfonyl fluoride (Beyotime ...
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bioRxiv - Microbiology 2021Quote: ... The suspension was treated with DNase I (1 μg ml-1) and RNase A (100 μg ml-1, Merck, India) to degrade nucleic acid residues of the host bacteria ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in 8 mL lysis buffer (5 mM imidazole in 100 mM potassium phosphate buffer pH 8.0) supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Neuroscience 2019Quote: ... anti-PSD-95 (Merck Millipore, cat 04-1066, 1:200; 1:1,000); anti-vGlut (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Penstrep and 1 ng/ml TGFβ (Cat nb. T7039, Merck, Sigma) or 20 ng/ml PDGF-AA (human PDGF-AA ...
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bioRxiv - Biophysics 2022Quote: ... cells were diluted in in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine to 50,000 cells/ml and plated at a density of 5000 cells per well (100 µl ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 1 mM PMSF and 1 X cOmplete protease inhibitors (Merck) and lysed with a French press under 1200 PSI ...
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bioRxiv - Immunology 2019Quote: ... The sections were fixed in a 1:1 mixture of methanol:acetone (Merck) and blocked with PBS containing 5% goat serum and 5% mouse serum (blocking solution) ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).
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bioRxiv - Biochemistry 2023Quote: ... A 1:1 mixture of magnetic hydrophobic and hydrophilic Seramag speedbeads (MERCK) were added at a 2:1 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were fixed 1:1 with 4% PFA (Merck, no. P6148) (final concentration 2% PFA ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 mM MgCl2 1 mM DTT and 1× cOmplete protease inhibitor (Merck)] were added and incubated on the rotating wheel for 2 h at 4ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL (1:500 dilution) of biotinylated anti-phosphotyrosine 4G10 (Merck) antibody and incubated for 1 hour ...
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bioRxiv - Genetics 2024Quote: Cells were lysed in 1% SDS plus 1:200 Benzonase (Merck, #E1014) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of RNA was reverse transcribed using a First Strand cDNA Synthesis Kit (K1622; Fermentas, Burlington, ON, Canada) and polymerase chain reaction (PCR) using a SYBR Green qPCR kit (Merck, Darmstadt, Germany) with incubation at 37°C for 60 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM FBP (Merck) (where applicable) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM dexamethasone (Merck), 5 μg/mL insulin (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... 1 NADPH (Merck-Millipore), and 0.2% Triton X-100 ...
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bioRxiv - Immunology 2021Quote: ... and 1 % methylcellulose (Merck). The PRRSV-2 16CB02 isolate (referred to as PRRSV-2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cyclopamine (1 μM, Merck) was added from day 2 to day 10 of differentiation ...
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bioRxiv - Biophysics 2019Quote: ... 1 mM PMSF (Merck) and a protease inhibitor cocktail (complete™ ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% NH4OH (Merck) right before infusion into the MS at a flow rate of 10 μL/min ...
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bioRxiv - Physiology 2022Quote: ... or Mdivi-1 (Merck) at the times and concentrations indicated in the figure’s legends.
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bioRxiv - Cancer Biology 2022Quote: ... 1 µM staurosporine (Merck), vehicle control or compounds from the small molecule library (Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT (MERCK, Sigma Aldrich ...