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Citations for Merck :
2201 - 2250 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysate was prepared and protein concentration was measured by Direct Detect® spectrometer (Merck). An equal amount of proteins (50 µg ...
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bioRxiv - Immunology 2024Quote: ... HLA-G2 proteins in PBS were concentrated by Amicon Ultra-15 (NMWL 10,000 Da) (Merck Millipore), and the flowthrough solution from protein concentration was used as a negative control (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein mixtures were incubated with 40μl PBST washed Streptavidin agarose resin (50% slurry, 69203-3 Merck) and incubated for 2h at room temperature on a roller ...
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bioRxiv - Bioengineering 2019Quote: ... 1×bugbuster and 1% lysonase (Merck, Hertfordshire, UK) in 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... RAD51 (Merck, Ab-1, PC130, dilution 1:1000), γH2AX (Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... then the acid supernatant was transferred to a 30 kDa centrifuge spin filter (Microcon-30 kDa Centrifugal Filter Unit with Ultracel-30 membrane, Merck, Australia; Product ID: MRCF0R030) prior to further centrifugation at 3,700 rpm for approximately 30 minutes until liquid had passed through the filter membrane ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of protein per sample were size-separated by electrophoresis on SDS-polyacrylamide gels and electroblotted onto Immobilon®-P polyvinylidene fluoride (PVDF) transfer membranes 0.45 μm pore size (Merck Millipore Ltd., Cork, IRL). After 1 h of blocking at room temperature with 5% skim milk in PBS 0.1% Tween ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
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bioRxiv - Pathology 2022Quote: ... 50 μg of protein was separated via SDS-polyacrylamide gel electrophoresis (SDS–PAGE; Bio–Rad Laboratories, Inc., Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... approximately 300 µL supernatant was passed through a 0.2 µm nylon membrane filter (AcroPrep™ Filter plate; Pall Corporation, New York, United States; or syringe filters; Merck & Cie KmG, Schaffhausen, Switzerland). Analysis with a sample volume of 40 µL was carried out using a Chromaster HPLC-System (Hitachi ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins in 65 μl of conditioned media and cell lysates per lane were separated on 6% polyacrylamide gels using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred on a poly-vinylidene difluoride (PVDF) membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA) using the MiniProteas Blotting Module (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... 2958 bp) was purified using commercial DNA purification kits (GenElute™ HP Plasmid Miniprep kit, Merck, #NA0160), according to standard procedures ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Biochemistry 2021Quote: ... the primary antibody (2.0µg/mL; anti-DNA:RNA hybrid, clone S9.6; monoclonal, mouse, IgG2ak, Merck Millipore, Germany) and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: For western blot and immunofluorescence analysis following primary antibodies were used: Anti-Actin (mouse, Merck Millipore); Anti-ARP3 (mouse ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass slides (Merck). After treatments cells were washed with PBS (3 times ...
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bioRxiv - Neuroscience 2023Quote: ... We dissected hippocampi from newborn mouse brain in ice-cold dissection media (HBSS (Merck Life Sciences) supplemented with 0.1 % w/v glucose ...
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bioRxiv - Developmental Biology 2021Quote: ... 1X complete protease inhibitor (Roche)] including 1% SDS and 1 U/µL benzonase (Merck). After 10 minutes of incubation at RT ...
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bioRxiv - Cell Biology 2021Quote: ... GLUT-1 (rabbit polyclonal, Merck Millipore, #400060, 1:100), Ki-67 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2022Quote: ... human mitochondria (clone 113-1, Merck Millipore, 1:100), and BrdU (clone BU1/75 ICR1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Puromycin (cat# MABE343, Merck Millipore; 1:2,000-1:10,000), anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263 ...
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bioRxiv - Biophysics 2022Quote: ... and 1 mg ml-1 lysozyme (Merck/Sigma–Aldrich). After 1 h incubation while stirring ...
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bioRxiv - Developmental Biology 2022Quote: ... 1/10 volume of 1% IGEPAL CA630 (Sigma, Merck) was added to the samples and incubated for additional 3 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mM AEBSF) and 1 protease inhibitor tablet (Merck). Lysis was done at 4 °C with the addition of 1 mg/ml lysozyme and further incubation at 4 °C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... anti-α-tubulin (Merck, 1:1000, TAT-1 00020911), anti-GFP (ChromoTek ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µM StemReginin 1 (SR1, Merck Millipore, 182706) for the indicated time points.
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bioRxiv - Microbiology 2020Quote: ... Proteins contained in BALF and TS were concentrated 5x using an Amicon Ultra column 100kDa (Merck Millipore) and 20 min centrifugation at 4000xg and samples were stored at −80°C ...
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bioRxiv - Biophysics 2020Quote: ... the protein was concentrated by centrifugation using Amicon Ultra 15-30k centrifugal filters (Merck, Kenilworth, NJ, USA) at 3452 x g ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... Target protein expression was detected using Immobilon™ Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
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bioRxiv - Bioengineering 2021Quote: ... the purified protein containing fraction was concentrated (Centriprep® Centrifugal Filters Ultacel® YM - 50, Merck Millipore).
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bioRxiv - Pathology 2021Quote: ... The proteins were cleaned using Amicon Ultra-4 Centrifugal Filter Devices (15 ml, 10 kD; Merck Millipore) to remove the elution buffer ...
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bioRxiv - Microbiology 2021Quote: ... Two microlitres of the supernatant was used for protein quantification using the Direct Detect system (Merck Millipore). The acquired concentrations of intracellular c-di-GMP were normalized to the total protein content.
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bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred on an Immobilon™-P Membran (PVDF, 0.45 µm, Art. No. IPVH00010, Merck Millipore) for 16 h at 15 V using a Mini Trans-Blot® Cell (Art ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded protein was concentrated with Amicon Ultra-15 Centrifugal Filter Units (100 kDa NMWL, Merck Millipore) and remaining urea removed with a PD10 desalting column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of the supernatant were used for protein quantification using the Direct Detect system (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... GGG-azide labelled proteins were concentrated to 25 μM on a 30 kDa Amicon Tube (Merck Millipore) in Tris/NaCl buffer and next labelled with 100 μM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Lentivirus stocks were concentrated 25-fold using centrifugal protein concentrators (Amicon Ultra, 10-kDa cutoff; Merck, Poland) and stored at - 80°C.
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... recombinant SARS-COV-2 Spike protein was desalted and concentrated with C4 ZipTips (Merck Millipore, Darmstadt, Germany) and spotted on a ground steel target using 2’,5’-Dihydroxyacetophenone ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were separated from each sample by centrifugation through Amicon ® Ultra Centrifugal Filters (Merck, Millipore Ltd.) at 21.000 x g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore ...
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bioRxiv - Developmental Biology 2019Quote: ... were transformed with the pET-UBE2D3 plasmids and protein expression was induced with 0.4 mM IPTG (Merck) for 3h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted proteins were collected after a 72-h incubation by Amicon Ultra-15 Centrifugal Filter Units (Merck) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was concentrated using an Amicon-Ultra-15 centrifugal filter (MWCO 30kDa) (Merck Millipore, USA), spin down 15 min in a bench-top centrifuge (4°C ...