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Citations for Merck :
2601 - 2650 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... and concentrated using Amicon concentrator devices (molecular weight cut-off, 10 000 and for full-length protein 50 000; Merck Millipore). Recombinant His-tagged proteins (MA4376 ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore) and afterwards labelled with 100 µM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Microbiology 2022Quote: ... and separating gel were used for SDS-PAGE and as a molecular weight standard protein marker (97, 66, 43, 29, 20, 14kDa) (Merck, India) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... the remainder was pre-cleared by co-incubation with 40 µL of PureProteome Protein A/G magnetic beads (Merck Millipore, LSKMAGAG10) per sample and rotation for 1 h at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... Standard immunostaining protocol was followed to stain for sarcomeric protein alpha-actinin and the coverslips were mounted on glass slides using fluoroshield (#F6182, Merck, Germany). Images were captured using the 60X oil immersion objective of the Olympus FV1000 confocal microscope (Olympus LifeScience ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The rest of the supernatant for each sample is used to measure the total protein level of the sample with a Bradford assay (Merck # B6916), following reagent protocol ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
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bioRxiv - Physiology 2023Quote: ... For protein extraction the tissue was homogenised in RIPA buffer with a cocktail of phosphatase inhibitors (Sigma-Aldrich, Merck, Gillingham, Dorset), using a sonicator three times for 30s each time ...
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bioRxiv - Neuroscience 2023Quote: ... or human post-mortem total protein/ synaptoneurosome samples were then mixed into equal volumes of 2X Laemmli buffer (Merck: S3401- 10VL) and boiled for 10 minutes at 98 °C ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Protein G Dynabeads (catalogue # 10003D) were washed with 0.02% (v/v) PBS-Tween 20 (Tween-20: catalogue # P2285, Merck; 0.01M PBS) and incubated with 200µL of primary antibody mix (for antibody details see Supplementary Table 5 ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... the elution of the biotinylated proteins was carried out boiling the beads in 25 μl of 3X protein loading buffer supplemented with 20 mM DTT and 2 mM Biotin (Merck, B4501) for 10 min at 95°C in gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were resolved on a 10% SDS-PAGE gel and Western blotting was performed using an anti-myc antibody (Merck, UK). Co-immunoprecipitation of Ssk2-HA was detected using an anti-HA antibody (Merck ...
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bioRxiv - Plant Biology 2021Quote: Multiple rounds of sequential extraction (initially by hexane/dichloromethane (1:1 v/v) (Merck, Germany ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:100 protease inhibitor cocktail and 1 mM Phenylmethylsulfonyl Fluoride (PMSF; all from Merck). Equal amounts of protein (40 μg ...
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bioRxiv - Immunology 2021Quote: ... or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000, ABS16, Merck Millipore) followed by incubation with respective HRP-conjugated secondary antibodies (G21040 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... with secondary antibodies diluted in blocking solution with 1% Hoechst 33258 (1:100, Merck). After three times washing with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 1% streptomycin and 2 mM of Glutamax and 0.1 μg·ml−1 GDNF (#SRP3200, Merck) (Vyas et al. ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl AAV virus was mixed with 1 μl 20% mannitol (MERCK K93152782 111). The virus and mannitol mixture were injected into a pulled-glass pipette (Warner Instruments ...
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bioRxiv - Genetics 2023Quote: ... and crosslinked with 1% formaldehyde in PBS buffer containing 1 mM Pefabloc SC (Merck), Complete proteinase inhibitor cocktail (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... phage stock was treated with 1 µL DNase I (10 U µL−1) (Merck) and 1 μL RNase A (10 U µL−1 ...
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bioRxiv - Microbiology 2023Quote: ... fixed cells were incubated with 1× PBS containing 1% bovine serum albumin (Merck KGaA) for 1 hour on ice ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we followed Magna-Chip™ A/G kit (from Merck) protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... ApopTag plus peroxidase in situ apoptosis detection kit (Merck Millipore) was used according to the manufacturer’s instructions.
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bioRxiv - Physiology 2019Quote: Plasma insulin was determined using a commercial kit (Merck Millipore) on a MAGPIXTM Multiplex reader and processed using Bio-Plex ManagerTM MP.
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bioRxiv - Bioengineering 2021Quote: Bromocresol Purple (BCP) Albumin Assay Kit (Sigma-Aldrich, Merck, Germany) performed as per manufacturer’s instructions with plasma samples diluted 5-fold in ultrapure water ...
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bioRxiv - Microbiology 2021Quote: ... following staining with a Gram stain kit (Merck, Darmstadt, Germany).
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Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanismbioRxiv - Biophysics 2022Quote: ... the ECL kit of Amersham (Merck, St Quentin-Fallavier, France) and the Amersham Imager 600 (GE ...
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bioRxiv - Developmental Biology 2022Quote: ... the ApopTag Red In Situ Apoptosis Detection Kit (Merck, S7165) was used ...
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bioRxiv - Neuroscience 2020Quote: The Black Gold II staining kit (Merck Millipore, Chengdu, China) was used according to the manufacturer’s instructions(Santiago Gonzalez et al ...
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bioRxiv - Molecular Biology 2022Quote: ... and libraries were extracted using GenElute plasmid miniprep kit (Merck).
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bioRxiv - Developmental Biology 2022Quote: The REDExtract-N-Amp™ Tissue PCR Kit (Merck, XNAT) was used for genotyping for all tissue explants ...
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bioRxiv - Molecular Biology 2024Quote: Duolink™ In Situ Probemaker PLUS kit (Merck, DUO92009-1KT) was applied to conjugate PLA oligonucleotides (PLUS ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed colonies were stained with the Alkaline Phosphatase kit (Merck) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Duolink™ In Situ Probemaker PLUS kit (Merck, DUO92009-1KT) was applied to conjugate PLA oligonucleotides (PLUS ...
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bioRxiv - Evolutionary Biology 2023Quote: A Magna RIP Kit (Cat# 17-704, Merck, Millipore, Germany) was used to perform the RIP assay following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The Anti-Citrulline (Modified) Detection Kit (cat. 17-347B, Merck) was used to measure global citrullination 88 ...
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bioRxiv - Plant Biology 2023Quote: A chromatin immuno-precipitation Kit (Merck Millipore, Burlington, MA, USA) was used to perform ChIP-qPCR assays ...
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bioRxiv - Developmental Biology 2023Quote: ... 900 μL of RIP Immunoprecipitation Buffer (Magna RIP kit, Merck) supplemented with 200 U RNaseIn was mixed with 100 μl of the lysate and this mixture was used to resuspend the antibody-coupled beads ...
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bioRxiv - Molecular Biology 2023Quote: A Chromatin Immunoprecipitation (ChIP) Assay Kit (Merck Millipore 17-295) was used for all CTCF ChIP experiments following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM MgCl (Merck KGaA), pH 8.1) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM MgCl (Merck KGaA), pH 8.1 ...