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Citations for Merck :
1951 - 2000 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of medium were filtrated on 0.45 µm PVDF membrane of Millex-HV® filter unit (Cat. No. SLHV R25 LS, MERCK KGaA), 3.5 ml of 4x PEG solution (32% PEG6000 ...
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bioRxiv - Plant Biology 2019Quote: ... and 10 µg (except for Fig S12 (3 µg)) was loaded on SDS-PAGE before transferring to PVDF membrane (Merck Millipore). SlMai1-myc or synSlMai1-myc proteins were detected using anti-Myc antibodies (GenScript ...
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bioRxiv - Bioengineering 2019Quote: Cell lysates were resolved by reducing SDS-PAGE and stained with colloidal Coomassie Brilliant Blue (Carl Roth) or transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore) using the TransBlot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were electrotransferred during a 30-min interval from the gel onto an Immobilon-P membrane of pore size of 0.45 µm (Merck Millipore, USA) under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Microbiology 2021Quote: ... structured microcosms were set-up in multi-well plates with a 0.22 µm filter membrane at the bottom of the well (Merck, Darmstadt, Germany) and containing 250 mg of sterile glass beads (with diameter of 80 to 120 µm ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Cell Biology 2022Quote: ... Purified chromatin was separated by SDS-PAGE and transferred to 0.45 μm polyvinylidene difluoride membranes (Immobilon, Millipore, Merck KGaA, Darmstadt, Germany). Blots were incubated with primary antibodies HDAC1 (Cell Signalling # D5C6U ...
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bioRxiv - Immunology 2022Quote: ... removed and faux adherent cells from the lower membrane washed into the bottom well before migrated cells were counted using a Guava EasyCyte 8HT Flow cytometer (Merck Millipore). Data was then presented as the total number of migrated cells or relative to vehicle controls (% control).
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bioRxiv - Immunology 2022Quote: ... resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Laemmli running buffer and transferred to PVDF membranes (Merck-Millipore) in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free supernatant (CFS) was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... and afterwards transferred to a methanol- activated Immobilon-P PVDF (poly-vinylidene difluoride) membrane (catalog#IPCVH00010, Merck Millipore Ltd., Carrigtwohill, Ireland) by tank blotting at 250mA for 90 min in a cold room (4 °C ...
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bioRxiv - Immunology 2024Quote: Net ALI membrane resistance for each sample was determined by measuring the sample resistance with a MillicellERS-2 Voltohmmeter (Merck Millipore) then subtracting the blank sample resistance reading ...
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Three microliters of cell suspension (whole cell) or cell lysate were spotted on an Immobilon-PSQ transfer membrane (Merck Millipore Ltd.) and air dried ...
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bioRxiv - Cell Biology 2023Quote: ... and the trophozoites were resuspended in 500 μl NaPBS/0.05 % saponin (or permeabilization of cell membranes (Sigma-Aldrich, Merck, Taufkirchen, Germany) and incubated for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Genomics 2022Quote: ... The NEBuilder reactions were dialyzed against water in a 6 cm Petri dish with a membrane filter MF-Millipore 0.05 µm (Merck, Kenilworth, NJ) for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... and the retina was separated from the eyes (see section Retina Isolation) and mounted ganglion-layer up on membrane filter (HAWP04700, Merck, USA). The retina was then secured with slice anchors (SHD-27LH/10 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the extracted liquid sample was collected by centrifugation through a filtered microcentrifuge tube (cellulose acetate membrane with pore size 0.45 μm; Merck CLS-8162), (x ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: Tumor slice and ear derma explants were then cultured on a 30-mm cell culture insert featuring a hydrophilic PTFE membrane (0.4 µm pore size, Merck Millipore #PICM0RG50) placed inside 6-well plates containing 1.1 mL of RPMI ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by the secondary DyLight 649 anti-mouse antibody (Merck Millipore, Darmstadt, Germany) or the Alexa Fluor 488 antibody (green) ...
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bioRxiv - Cell Biology 2019Quote: ... samples were incubated with 53BP1 antibody (anti-53BP1, mouse monoclonal, Merck, Darmstadt, Germany) followed by a secondary fluorescent antibody (AlexaFluor-488 ...
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bioRxiv - Cell Biology 2021Quote: ... The following secondary antibodies were used: rabbit anti-mouse-HRP (A9044) from Merck, goat anti-rabbit-HRP (ab6721 ...
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bioRxiv - Molecular Biology 2022Quote: ... Digoxygenin (DIG) probes were detected after hybridization using mouse anti-DIG (11333062910; Merck) and anti-mouse Alexa Fluor 488 (A-11001 ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibody and with mouse monoclonal anti-map2 primary antibody (Merck Millipore, MAB3418). The secondary antibodies used were the anti-Rabbit Alexa Fluor 546 (Thermo fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: anti-FMRP mouse monoclonal (MAB2160, Merck-Millipore), anti-OCT-4 rabbit polyclonal (sc-9081 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The mouse anti-coronavirus monoclonal antibody MAB9013 was purchased from Merck (Darmstadt, Germany). The secondary antibody peroxidase-conjugated AffiniPure F (ab’)2 Fragment Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2019Quote: ... The antibodies used were: mouse anti-H2Ax pSer319 (γH2Ax; JBW301; 1;1000 dilution; Merck) and Alexa-568 anti-mouse IgG (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies included: goat anti-mouse IgG antibody (Merck Millipore AP308P, (H+L) HRP conjugate ...
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bioRxiv - Microbiology 2023Quote: ... The mouse mAb that detects the IAV nucleoprotein was purchased from Merck (MAB8257). Secondary antibodies conjugated to AF405 and AF488 were purchased from Molecular Probes.
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated anti-rabbit and HRP-conjugated anti-mouse (RABHRP1 and RABHRP2, Merck); MOWIOL 4-88 Reagent (475904 ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used: Glutamine Synthetase (monoclonal mouse, MAB302, Merck Millipore, USA), NeuN (monoclonal mouse ...
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bioRxiv - Cell Biology 2024Quote: ... using the following primary antibodies: mouse anti-RAD2l (0S-908, Merck Millipore, l:S00), rabbit anti-SMC2 (abl04l2 ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant containing Matrigel proteins was discarded and pellets were lyzed in RIPA buffer (Merck), supplied with protease and phosphatase inhibitors (Merck) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Developmental Biology 2020Quote: hESCs were harvested for protein extraction by addition of CelLytic M lysis buffer (Merck), freshly supplemented with protease inhibitors (PIC ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were concentrated with an Amicon Ultra-15 10 kDa centrifugal filter unit (Merck). Protein concentration was determined by UV-spectroscopy.
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bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Bioengineering 2021Quote: ... the cDNA of the fusion protein was cloned in pET21a (Merck KGaA, Darmstadt, Germany). For preparative overexpression the E ...
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bioRxiv - Bioengineering 2021Quote: ... and supplemented with 10 µM/ml Rho-associated protein kinase inhibitor (Y-27632, Merck) for the first day.