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Citations for Merck :
2101 - 2150 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated to 12 mg/mL using an Amicon Ultra Centrifugal Filter (Merck-Millipore). To purify non-degraded PrgA (without the S476A mutation) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Ultra-15 mL centrifugal filter devices (Amicon, Merck KGaA, Darmstadt, Germany) with a molecular weight cut-off (MWCO ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein was concentrated to ∼ 500uL using Amicon Ultra 5 centrifugal filters (Merck, Darmstadt, Germany). The affinity purified protein was further separated by gel filtration using a Phenomenex Yarra 3µm SEC-4000 300 x 7.8mm column (Phenomenex ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM Cdc5Plk1 re-phosphorylated Mif2 protein (M3) was immobilized on anti-FlagM2 beads (Merck) and incubated with 25 µM Ame1/Okp1 complex in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were concentrated to 50−100 µM using the Amicon Ultra centrifugal filters (Merck). For protein labelling ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Developmental Biology 2022Quote: ... The eluted proteins were then purified by Fractogel® EMD TMAE (Merck KGaA, Darmstadt, Germany) or SP-Sepharose (GE-Healthcare ...
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bioRxiv - Cell Biology 2022Quote: All proteins used in this study were overexpressed in Rosetta (DE3, pLysS) Escherichia coli (MERCK) cells using 0.4 mM isopropyl 1-thio-D-galactopyranoside (Inalco Pharmaceuticals ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore). The purity of the protein was analyzed by SDS-page and Western-blot using Anti-His antibodies.
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Synthetic Biology 2020Quote: ... eluted protein fractions were concentrated using Amicon® Ultra-15 centrifugal filter units (Merck, USA) with a 100 KDa cut-off ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the purified proteins were concentrated with Amicon Ultra-0.5 Centrifugal Filter devices (Merck Millipore) following manufactures instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated using a 30 kDa MWCO centrifugal filters (Amicon Ultra; Merck Millipore) and then gel filtered ...
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bioRxiv - Neuroscience 2021Quote: ... as confirmed by the anti-glial fibrillary acid protein (anti-GFAP) antibody (Merck, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2022Quote: ... L1s were then broken up to extract proteins using a Beadbug homogenizer (Merck Life Science) at the highest setting for 90 seconds three times at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Developmental Biology 2022Quote: ... Protein bands were visualised using a chemiluminescence substrate (Immobilon Forte, Merck Life Science UK Ltd.) on the ChemiDoc Imaging system (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... and protein complex was eluted in lysis buffer 2 containing 2.5 mM D-desthiobiotin (Merck). Protein tags were removed by overnight cleavage with HRV 3C protease ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were then desalted and concentrated by ultrafiltration with Amicon Ultra 0.5 10K (Merck Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... Total protein was extracted using RIPA buffer mixed with protease inhibitor cocktail (Millipore Merck, 539134) and quantified using Qubit® Protein Assay Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... In brief supernatant protein concentration was standardised prior to being concentrated using Microcon® (Merck) 10Kda filter columns and centrifugation (14,000g 20 minutes) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using an Amicon Ultra-15 10K MWCO Centrifugal Filter Unit (Merck Millipore). Protein and ligand samples were degassed prior to each ITC experiment ...
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bioRxiv - Cell Biology 2024Quote: ... The chromatin (100 μg) was precleared with protein A agarose/Salmon sperm DNA beads (Merck) and 2 % taken as input ...
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bioRxiv - Biochemistry 2024Quote: ... Glycerol-free eluted protein was concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (Merck) at 10,000 x g ...
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bioRxiv - Plant Biology 2022Quote: ... 100 μg of protein were loaded into FASP filters (Amicon Ultra 30K, 0.5 ml, Merck) and tryptically digested over night following the FASP (Filter-Aided Sample Preparation ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced for 10 mins at 60°C with 10 mM dithiothreitol (Sigma, Merck) in 50 mM ammonium bicarbonate and then alkylated with 55 mM iodoacetamide (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 75 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... Dialyzed proteins were concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and further purified by size-exclusion chromatography using a Superdex 200 (16/600 ...
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bioRxiv - Immunology 2023Quote: ... EVA71 VP0/VP2 reactive proteins were detected using anti-EVA71 VP2 antibody clone 979 (Merck) at 1:2000 dilution and anti-mouse IgG HRP conjugate ...
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bioRxiv - Cell Biology 2023Quote: Protein extracts were transferred into an Amicon Ultra Centrifugal filter device (10 kDa MWCO, Merck) for buffer exchange [50 mM Tris-HCL pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... and the protein concentration was determined using a Direct Detect® Infrared Spectrometer (Millipore, Merck).
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bioRxiv - Biophysics 2024Quote: ... The dissolved protein solution was then repeatedly buffer exchanged with a centrifugal filter (Amicon, Merck) against the same buffer to eliminate the residual TFA ...
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bioRxiv - Biochemistry 2024Quote: ... Each of the purified proteins was concentrated by an Amicon Ultra centrifugal filter units (Merck) with an appropriate molecular weight cutoff followed by filtration through a 0.45 μm pore size membrane filter (Merck) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% glutamine and 1% penicillin/streptomycin (Merck). Fascin KD cells were selected and maintained using puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... GluA1 (1:250-1:500; Merck Millipore) and RTP801 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Cell Biology 2022Quote: ... the SDS samples were applied onto an Amicon filter unit (MWCO = 10 kDa; Ultracel-10 cellulose membrane, Cat.No; UFC201024, Merck Millipore, Billerica, MA, USA) to trap the proteins ...
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and HA-tagged proteins of samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a PVDF membrane (Merck Millipore, Tokyo, Japan). The membrane was incubated with rabbit anti-N antiserum or mouse anti-GFP antibody ...
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bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
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bioRxiv - Biochemistry 2019Quote: ... The active fractions post-purification was pooled and concentrated using 30 kDa cut-off size membranes of Amicon-Ultra-15 (Merck Millipore, Bangalore, India). Plasmid purification and gel purification kits were obtained from Qiagen (Hilden ...
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bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
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bioRxiv - Cell Biology 2020Quote: ... were separated with a 10% SDS-PAGE resolving gel using the Laemmeli discontinuous buffer system and then transferred to a PVDF transfer membrane (Immobilon-FL, Merck KGaA, Darmstadt, Germany) using a Biorad wet transfer apparatus ...