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Citations for Merck :
1801 - 1850 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein (30-40 μg/sample) were resolved on a 10% polyacrylamide gel and transferred onto a PVDF membrane (Immobilon-E, Merck). The membrane was blocked with 5% milk in TBS containing 0.1% Tween20 (TBS-T ...
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bioRxiv - Microbiology 2019Quote: ... balechii culture was collected from each treatment and was filtered using a 0.22-μm nitrocellulose membrane (Merck Millipore, Darmstadt, Germany). The membrane was then transferred into a 2-mL micro-centrifuge tube containing 1 mL DNA lysis buffer (containing 0.1 M EDTA ...
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bioRxiv - Microbiology 2019Quote: A sample (10 mL) was removed from the Gambierdiscus culture and filtered through 3-μm membrane (Merck Millipore, Darmstadt, Germany). One hundred microliters of the filtrate ...
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bioRxiv - Cell Biology 2021Quote: Proteins in 2× SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... and were transferred at 100 mA for 70 min onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using semi-dry blotting system ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... round bottomed deep well plate at 40 % liquid volume and sealed with a Breathe-easy sealing membrane (Merck, #2380059-1PAK). Overnight growth occurred in an Infors MT multitron pro shaker at 250 RPM rotating orbitally at 25 mm diametres at 37 °C for 17 h.
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bioRxiv - Plant Biology 2021Quote: ... The solution was then dried using a speed vacuum (Centrivap concentrator, Labconco) and passed through a 0.2-mm membrane filter (Millex-GV filter unit, Merck Millipore). The filtrate was used for sucrose ...
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bioRxiv - Plant Biology 2020Quote: Total protein extracts were separated by SDS PAGE on an acrylamide gel before being electrotransferred on a PVDF membrane (0.45 μM, Merck millipore). After saturation in a (1X TBS −0.1% tween - 5% fat-free dry milk ...
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bioRxiv - Microbiology 2021Quote: ... Equal volumes of total lysates were analyzed using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore). Rabbit anti-BMV 1a antiserum (1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: The samples were loaded onto a 5–20% gradient SDS-PAGE gel (Wako, Osaka, Japan) and transferred to an Immobilon-P Transfer Membrane (Merck). Antibodies were diluted with Signal Enhancer HIKARI for Western Blotting and ELISA (Nacalai Tesque) ...
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bioRxiv - Genomics 2022Quote: ... 10 ml seawater sample collected from the same station was passed through a 0.22 µm membrane filter to remove any cellular organisms (Isopore™ 0.2 µm GTTP; Merck, Ireland) (31) ...
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bioRxiv - Microbiology 2022Quote: The MSM media containing residual CP after the photo-Fenton process was filter sterilized using a 0.22 μm membrane filter (Merck, Germany). To this media ...
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bioRxiv - Biophysics 2022Quote: ... The elution volume of 60-70 mL was collected and the solution was concentrated to a volume of 200 μL with an Amicon Ultra-15 PLHK Ultracel-PL Membrane 100 kDa (Merck) spin column ...
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bioRxiv - Bioengineering 2022Quote: ... Viral supernatant was harvested at 48 and 72 h post-transfection and filtered through a 0.45 μm PVDF membrane (Merck Millipore). For lentiviral infection ...
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bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Immunology 2021Quote: ... and filtered through a 0.22 µM membrane The concentration of purified RBD was determined using the Bradford assay (Merck, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The five eluted aliquots were then concentrated using an ultrafiltration device with a 3 kDa cut-off membrane (Amicon Ultra-0.5 Centrifugal Filter Unit; Merck, UK) and desalted with filtered water to remove urea and salts ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ssDNA was eluted and concentrated using an ultrafiltration device with a 3 kDa cut-off membrane (Amicon Ultra-0.5 Centrifugal Filter Unit; Merck, UK), as described earlier ...
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bioRxiv - Neuroscience 2022Quote: ... 25 μg of protein were resolved by 8% dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrophoretically transferred to a 0.45 μm polyvinylidene difluoride membrane (PVDF; Immobilon, Merck Millipore). Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... the sporozoites were separated from unhatched oocysts by filtration using 3.0 µm nitrocellulose membranes (Merck Millipore Ltd., County Cork, Ireland). Fluorescence of filtered samples was evaluated ...
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bioRxiv - Biochemistry 2019Quote: ... the media containing secreted mTLR9-ECD was concentrated by tangential-flow filtration on a 30 kDa cut-off membrane (Merck). mTLR9-ECD was purified by protein A-affinity chromatography with IgG Sepharose 6 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... Fractions 7-9 were individually collected (500 μL/fraction) and concentrated by Amicon Ultra-0.5 centrifugal filter unit with 10 kDa membrane (Merck Millipore) with 14,000 x g at 4 °C for 8 min centrifugation to have final volume less than 100 μL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204, Merck Millipore) and probed with the respective primary and secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The purest and most concentrated fractions were then dialyzed by employing a 3 kDa membrane D-Tube Dialyzer (Merck, Germany) in PBS buffer (200 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... donkey serum TBS IHC wash buffer and filtered using a centrifugal filter with a 0.1 mm PVDF membrane (Ultrafree-MC, Merck Millipore). Sections were stained with the antibody mix ...
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bioRxiv - Biochemistry 2019Quote: ... ∼500-ml cell suspension containing the secreted IgG was clarified by centrifugation and subsequent filtration through a 0.45 µm HAWP membrane (Merck Millipore). The filtrate was mixed with an equal volume of binding buffer (20 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Microbiology 2019Quote: ... three aliquots of 500 mL of well-mixed seawater taken from the field were filtered through a 0.22 μm mixed cellulose esters membrane (Merck Millipore) using sterile techniques ...
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bioRxiv - Immunology 2020Quote: ... MS2-FP8 VLPs were concentrated from SEC purified fractions by ultra-centrifugal filtration using Amicon® Ultra-15.0mL 100 K membrane (Merck Millipore Ltd. ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 5.8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol ...
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bioRxiv - Biochemistry 2021Quote: ... adjusted to pH 8 using 3 M HCl and filtered with 0.2 μm nylon membrane filters (GNWP04700, 0.2 μm pore size, Merck Millipore Ltd.), while mobile phase B consisted of 100% methanol (method described in Table 2) ...
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bioRxiv - Cancer Biology 2020Quote: ... Gaithersburg, MD, USA) and 8-μm-pore polycarbonate membranes (Nucleopore, Pleasanton, CA, USA) coated with 10 μg/ml fibronectin (Merck) as described elsewhere (Prevete et al. ...
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bioRxiv - Microbiology 2021Quote: ... the aqueous phase was filtered through a Sterivex™-GP 0.22 μm polycarbonate membrane filter unit (Merck Milipore, South Africa) followed by phenol:chloroform DNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... samples were fixed in 2.5% (v/v) glutaraldehyde in HBSS on IsoporeTM membrane TMTP filters with pore size 5 µm (Merck-Millipore) for 30 min followed by washing thrice with HBSS buffer (10 min each) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was collected after 48 h and 72 h and filtered through a 0.45 µm PVDF membrane (Merck Millipore).
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bioRxiv - Microbiology 2022Quote: ... ZIKV was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-100 membrane (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The eluted aliquots were then desalted with filtered water and concentrated using an ultrafiltration device with a 3 kDa cut-off membrane (Amicon Ultra-0.5 Centrifugal Filter Unit; Merck, UK) to remove any urea and salt residues.
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bioRxiv - Physiology 2024Quote: ... the excess of reagents was eliminated by performing several washing steps with Milli-Q water using 4 mL cellulose membrane centrifugal filters (Amicon, MilliPore, 100 kDa, Merck). All MNP suspensions were sterilised by filtration using 0.22 μm MilliPore® filters before addition to cell cultures ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
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bioRxiv - Neuroscience 2024Quote: ... were plated for neuronal differentiation in wells pre-coated with Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Merck). Cells were plated in NMM and half the media was replaced twice a week ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissociated follicles were kept on the Transwell-COL membranes contacted with IVG medium composed of αMEM supplemented with 2% polyvinylpyrrolidone (PVP360, Merck), 5% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Molecular Biology 2023Quote: ... Switzerland) and further processed by tangential flow filtration (30 kDa MWCO, PES membrane; Pellicon XL 50; Millipore, Merck, Darmstadt, Germany), autoclaved ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were loaded in SDS-PAGE and transferred to Immobilon®-PSQ Transfer Membrane (0.2 μM, Merck Millipore Ltd.) in 1x transfer buffer using a Bio-Rad transfer apparatus ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...