Labshake search
Citations for Merck :
1601 - 1650 of 1811 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The flow-through fraction was collected and concentrated using an Amicon Ultra 10 kDa molecular weight cutoff centrifugal filter unit (Merck Millipore). The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences) ...
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bioRxiv - Bioengineering 2024Quote: ... The insoluble fraction was resuspended in PBS 1X and the supernatant was loaded in an Amicon Ultra 10kDa filter (Merck, Germany). The supernatant was centrifugated at 4000 g at 4°C for concentration and buffer exchange with 1 mL of equilibrium buffer containing 50 mM phosphate buffer pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the protein of interest were pooled and concentrated using an Amicon® Ultra-4 3K centrifugal filter unit (Merck) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2023Quote: ... Clear lysates were subsequently filtered through 10 kDa followed by 3 kDa Amicon Ultra-0.5 centrifugal filters (Merck KGaA, Darmstadt, Germany) to generate sub-cellular fractions with molecular size of larger than 10 kDa ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 32P-γ-ATP was removed by washing with 3 column volumes of Millipore water and centrifugation in 10 kDa (Qβ-RNA) or 3 kDa (8mer) Amicon filters (Merck Millipore) at 14,000 rpm at 4 °C for four times ...
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bioRxiv - Systems Biology 2023Quote: ... Lysates were centrifugated 5 minutes at 15,000 g and 500 µL of supernatant were concentrated using an Amicon® Ultra 30 kDa filter (Merck Millipore) following centrifugation at 14,000 g for 20 minutes ...
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bioRxiv - Biophysics 2023Quote: ... and fractions containing histone octamers were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). The protein concentration was determined with Bio-Rad Protein Assay Dye Reagent (Bio-Rad # 5000006).
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bioRxiv - Biophysics 2023Quote: Rec samples were transferred to a labeling buffer (50 mM HEPES, pH=7.0, 5 mM EGTA) using 10 kDa MWCO Amicon Ultra centrifugal filter unit (Merck Millipore, USA), and concentrated to 0.5 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... These two peak fractions were pooled separately and concentrated on an Amicon Ultra Centrifugal Filter with a 30 kDa cut-off (Merck-Millipore). 10% glycerol was added to the concentrated protein fractions ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting sample of holo-SmLPMO10A was concentrated to ~ 150 μM using an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck) and the sample was split into three equal volumes of ~ 500 μL ...
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bioRxiv - Genomics 2022Quote: ... The NEBuilder reactions were dialyzed against water in a 6 cm Petri dish with a membrane filter MF-Millipore 0.05 µm (Merck, Kenilworth, NJ) for 1 hr ...
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bioRxiv - Immunology 2023Quote: ... ADU-S100 was obtained from Oxeltis(Montpellier, France) and spin filtered using Amicon Ultra4 centrifugal filter tubes (Merck-Millipore, Watford, UK). Absence of endotoxins (<0.5 EU/mg ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each scFv-Fc was buffer-exchanged using a Merck™ Amicon™ Ultra Centrifugal Filter tube (Merck™, cat no. 10581342) in sterile PBS ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was re-applied onto Ni-NTA Superflow resin and the flow-through was pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was re-applied onto the Ni-NTA Superflow cartridges and the flow-through was pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S75 (26/600 ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted at 460 g at 4 °C for 30 s and concentrated in a 1% GDN pre-equilibrated 30 kDa Amicon Ultra centrifugal filter (Merck Millipore). The concentrated eluate was spun through a Zeba Spin Desalting Column 40 kDa MWCO (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions corresponding to the peak at the elution volume ∼1.3 mL were pooled together (total volume 300 µL) and concentrated to 30 µL using a 100000 Da molecular weight cut-off centrifugal filter (Merck Millipore). n-Octyl-β-D-Glucoside was freshly added to the sample to a final concentration of 0.07% m/V.
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Neuroscience 2023Quote: ... and the retina was separated from the eyes (see section Retina Isolation) and mounted ganglion-layer up on membrane filter (HAWP04700, Merck, USA). The retina was then secured with slice anchors (SHD-27LH/10 ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted fractions were concentrated and dialyzed against PBS using Amicon Ultra-15 Centrifugal Filter Units with a 3 kDa MWCO (Merck Millipore). Endotoxins were removed using High Capacity Endotoxin Removal Spin Columns (Pierce) ...
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bioRxiv - Biochemistry 2023Quote: ... Filter-aided sample preparation (FASP) was performed using a 30 kDa molecular weight cutoff centrifugal filters (Microcon 30, Ultracel YM-30, Merck Millipore) as previously described59 ...
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Immunology 2023Quote: ... Cell supernatants were centrifuged at 500 ×g for 5 min to remove dead cells and concentrated with 10 kDa MW cut-off filters (Amicon, Merck Millipore), as described by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... filtered cell culture supernatant containing infectious HCV was concentrated 50x using 100 kDa Amicon Ultra-15 centrifugal filter units (Merck Millipore) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were dialyzed overnight against 10 mM phosphate buffer (pH 7.5) and filtered with Ultrafree-MC-GV Centrifugal Filters (Durapore-PVDF, 0.22 μm) (Merck KGaA, Darmstadt, Germany). Filtered samples containing 0.15-0.20 mg/ml of protein were loaded into a 1-mm quartz cuvette (Hellma ...
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bioRxiv - Cancer Biology 2023Quote: ... the unencapsulated FITC-dextran or FITC-SAP was removed by washing and filtration using Amicon Ultra centrifugal filters (100 K device, Merck Millipore). The incorporation of FITC-dextran and FITC-SAP into the nsEVs was confirmed by spectrofluometer analysis (FP-6200 ...
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bioRxiv - Biophysics 2023Quote: ... used for protein-dilution was degassed for 0.5 hr and Opti-MEM™ medium was filtered twice with 0.45 μm syringe filter (Merck Millipore millex) before starting the transfection procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... and dialyzed against cold sterile PBS using an Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa MWCO (Merck Millipore). The resulting product was then passed through a 0.22 µM filter.
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bioRxiv - Biochemistry 2024Quote: ... The fractions were analysed for protein content and impurities using SDS-PAGE and then concentrated to ∼100 μM using 10kDa or 30kDa MWCO concentrators (Amicon® Ultra Filters, Merck). The protein was aliquoted into 20 μL volumes ...
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bioRxiv - Neuroscience 2024Quote: ... The medium was refreshed the next day and virus-containing medium was collected and concentrated 72 hours post-refreshing using Amicon Ultra 15 filters (C7715, Merck Millipore).
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Plant Biology 2024Quote: ... Excess dye was removed by loading the labelling mix into a column (Amicon® Ultra 0.5 centrifugal filter devices 3K; Merck UFC500324) and centrifuged for 8 minutes at 10,000 x g ...
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bioRxiv - Bioengineering 2024Quote: ... Laminin 411 (Biolamina, #LN411) and Laminin 111 (Biolamina, #LN111) were first purified using Amicon Ultra-0.5 centrifugal filter units (Merck Millipore, UFC5003) and reconstituted in sterile PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing the protein of interest were pooled and concentrated using Amicon Ultra-15 centrifugal filter units with a 100-kDa molecular weight cutoff (Merck Millipore) Concurrently ...
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bioRxiv - Microbiology 2024Quote: ... with a 7K molecular weight cut-off (MWCO) and in some cases further concentrated using Amicon Ultra Centrifugal Filter Units devices (100 kDa MWCO, Merck Millipore) to sample concentrations of ∼0.1-1.1 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... The filtered supernatant was concentrated to 125 μL using an Amicon Ultra-15 (molecular weight cut-off 100 kDa) centrifugal filter device (Merck Millipore), which was centrifuged at 4,000 × g for 60 minutes at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... three washing steps were performed with Millipore water using Amicon Ultra-0.5 centrifugal filters 50 kDa MWCO (Merck KGaA, Darmstadt, Germany). Concentration of final stock solutions (ranging from 170 to 300 g/l ...
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bioRxiv - Systems Biology 2024Quote: Glass beads from the pre-prepared stock solution were diluted in acetonitrile (6.25 mg/mL) and this bead slurry was dispensed into MultiScreen deep filter plates (Merck Millipore, MDRLN0410) (800 µL/well) ...
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bioRxiv - Microbiology 2024Quote: Sucrose gradient purified fractions of bioreactor-derived PV-2 SC6b and PV-2 SC5a were pooled and concentrated with buffer exchange into PBS + 20mM EDTA (pH 7) using Amicon Ultra centrifugal filter devices (100 kDa MWCO, Merck Millipore) to a final concentration of 0.1 mg/ml (PV-2 SC6b ...
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bioRxiv - Immunology 2024Quote: ... and EV- containing fractions were pooled and concentrated to 2 ml with 10 kDa molecular weight cut-off Amicon centrifugal filter units (Merck Millipore). For the proteomic analysis ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was immediately separated from the cells by filtration through a 0.45-µm pore size filter (PVD membrane, Merck Millipore Ltd.) ((adapted from [59]).
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bioRxiv - Immunology 2024Quote: ... The melanin ghost suspension was then washed with sterile PBS and placed in 0.22 µm centrifugal filter units (Merck Millipore Ltd). The melanin ghosts were then washed 3 times and resuspended in deionized water ...
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bioRxiv - Biochemistry 2024Quote: ... and the fractions with the highest concentrations of mutant PGA proteins were concentrated into PBS using Amicon Ultra-4 Centrifugal Filters with a 10 kDa molecular weight cutoff (Merck Millipore). This process resulted in a protein with a purity exceeding 70 % and a concentration of approximately 1 mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... the eluate was reduced to approximately 40–50 ml and then concentrated using an Amicon Ultra-15 10 kDa molecular mass cut-off spin filter (Merck Millipore). Final EV preparations were kept in sterile filtered PBS for immediate use or sterile PBS-HAT buffer56 for storage at −80℃.
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bioRxiv - Cell Biology 2024Quote: ... The cells were collected by scratching the plates 72 h post-transfection and filtered using an Amicon Ultra-0.5 centrifugal filter (Merck Millipore, UFC510024). Extraviral DNA was removed by digestion with DNase I (Thermo Scientific ...