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Citations for Merck :
1401 - 1450 of 1811 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The eluted samples were subject to immediate concentration with concomitant buffer exchange with fresh PBS to remove imidazole using Amicon centrifugal filters (Merck). For hACE2 ...
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bioRxiv - Microbiology 2021Quote: ... the aqueous phase was filtered through a Sterivex™-GP 0.22 μm polycarbonate membrane filter unit (Merck Milipore, South Africa) followed by phenol:chloroform DNA extraction ...
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bioRxiv - Immunology 2021Quote: Culturing medium was changed 24 h post-transfection and vector supernatants were collected 48 h after transfection and processed by centrifugation at 1000 g for 10 mins at 4°C to remove cellular debris followed by microfiltration using Millex-HV 0.45 μm syringe filter units (Merck – SLHV033RB). Viral supernatants were either kept on ice for further use or frozen down at −80°C for storage.
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bioRxiv - Immunology 2021Quote: ... 300 µL of saliva sample was mixed with 200 µL of 100 mM Tris-HCl buffer (pH 8.5; Nippon Gene Co., Ltd.) in an Amicon Ultra-0.5 Centrifugal Filter Unit (3 kDa; Merck Millipore Ltd), and the mixture was spun down at 14,000 g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Eluted fractions containing HCA II were pooled and concentrated to ~20 mg/ml by using Amicon Ultra Centrifugal Filter Units (Merck) with a molecular weight cut-off 10 kDa.
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bioRxiv - Immunology 2021Quote: All samples for CD-MS measurements were first buffer exchanged into 500 mM ammonium acetate solution (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore), unless otherwise stated ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... in HEPES buffer was exchanged with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0) using YM30K centrifugal filter unit (Amicon ultra-0.5 mL, Regenerated cellulose, Merck Millipore Ltd.,). It was further diluted with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0 ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were pooled for mass spectrometric analysis and concentrated with an Amicon Ultra – 0.5mL 100-kDa cut-off centrifugal filter unit (Merck Millipore) for cryo-EM sample preparation (1.7 mg ml-1 Cav1.2(ΔC)/Cavβ3 sample or 2.7 mg/ml Cav1.2(DC)/Cavβ3/Cavα2δ sample).
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bioRxiv - Biophysics 2022Quote: ... CM was concentrated ≈300 times to 0.5 mL using a Centricon Plus-70 centrifugal filter device with a 10K nominal molecular weight limit (Merck Millipore).
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bioRxiv - Biophysics 2022Quote: ... Fraction 4-6 of three different columns were pooled and concentrated to 0.5 mL using an Amicon Ultra-15 10K filter (Merck Millipore). This was then placed on top of the gradient.
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bioRxiv - Evolutionary Biology 2022Quote: ... Hyporheic water samples of 250–500 ml were filtered using a 50-ml sterile syringe (Terumo) and 0.22-μm Sterivex™ filters (Merck Millipore ...
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bioRxiv - Microbiology 2022Quote: ... ZIKV was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-100 membrane (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2022Quote: ... or 300 mM (octamer-mix + APLFAD) ammonium acetate at pH 7.5 using 3 kDa MWCO Amicon Ultra Centrifugal Filter Units (Merck Millipore). After buffer exchange the volume of each sample was ∼40 µL ...
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bioRxiv - Microbiology 2023Quote: ... Germany) followed by size-fractionation using a 3 kDa centrifugal filter according to the manufacturer’s instructions (Merck KGaA, Darmstadt, Germany). The samples were kept at 4 °C or on ice throughout the process ...
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bioRxiv - Microbiology 2023Quote: ... Clarification was then performed by centrifugation for 1 hour at 12,000g and 4°C and vacuum filtration using 45um nylon filter systems (SteriFlip - Merck Millipore). Prior to purification ...
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bioRxiv - Molecular Biology 2023Quote: ... Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 μm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The eluted aliquots were then desalted with filtered water and concentrated using an ultrafiltration device with a 3 kDa cut-off membrane (Amicon Ultra-0.5 Centrifugal Filter Unit; Merck, UK) to remove any urea and salt residues.
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bioRxiv - Microbiology 2022Quote: The sterile filtered fecal supernatant was concentrated using Centriprep® filter units (Merck, cat. no. 4311 and cat. no. 4307). This constituted the concentrated virome ...
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bioRxiv - Microbiology 2022Quote: ... through six dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with 10 kDa molecular weight cutoff (Merck). For experiments studying the IgG-binding properties of Fc-III and Scr ...
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bioRxiv - Biophysics 2022Quote: ... The protein was dialyzed in 20 mM phosphate pH 7.4 and 500 mM NaCl to remove imidazole and concentrated using a 10 kDa Amicon® Ultra Centrifugal Filter (Merck).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... from the resin was immediately neutralized with 1/10 faction volume of 1 M Tris-HCl pH 8.5 and concentrated using an Amicon Ultra-2 Centrifugal Filter Unit 100kDa NWMCO (Merck Millipore), in which the elution buffer was exchanged with PBS (GIBCO).
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bioRxiv - Physiology 2024Quote: ... the excess of reagents was eliminated by performing several washing steps with Milli-Q water using 4 mL cellulose membrane centrifugal filters (Amicon, MilliPore, 100 kDa, Merck). All MNP suspensions were sterilised by filtration using 0.22 μm MilliPore® filters before addition to cell cultures ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer was exchanged for 1× DPBS and LNPs were concentrated by centrifugation on Amicon 50 kDa filter unit (Merck #UFC805024). Size distribution and polydispersity index were determined using dynamic light scattering on Zetasizer Ultra Red (Malvern ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
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bioRxiv - Molecular Biology 2024Quote: ... was filtered through a 0.45 μm syringe filter and 50-60× concentrated using Amicon Ultra-15 100 kDa NMWCO columns (#UFC910024, Merck Millipore). The resulting concentrate was aliquoted and stored at -80°C until further use.
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bioRxiv - Microbiology 2023Quote: ... 1 L of freshly 0.1 µm filter-sterilised seawater (n = 2) was filtered onto a 0.2 µm Sterivex (Merck Pty Ltd) filter and snap-frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... The next day the cleaved protein was collected and concentrated by spinning the sample at 3500 RPM at 4°C using −50kDa centrifugal filter tube (Amicon Ultra-15, Merck). The protein was purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE28-9909-44 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell culture supernatants from 4950T or 170162T cells were concentrated 10x using Amicon centrifugal filtration units with 100 kDa cutoff filter (Merck). Concentrated supernatant or glycodelin A isolated from amniotic fluid was added to the lectin coupled beads and incubated overnight on an overhead shaker at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... A 6:4 ratio of 0.1 % Oil-Red-O in isopropanol to 4 mL distilled water was prepared and passed through a 0.22 □m syringe filter (Merck: Z260479) before use ...
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bioRxiv - Biochemistry 2024Quote: ... The purified solutions were concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, USA). For protein concentration determination ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM DTT and 5%(v/v) glycerol by five cycles of filtration through 10 kDa Amicon filters (Merck Millipore), and finally concentrated to 0.5 mg/ml.
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Biochemistry 2024Quote: Exoproteome fractions were concentrated to 1 mg mL-1 total protein (approximately 10 times concentration) using an Amicon Ultra-0.5 Centrifugal Filter Unit with a molecular weight cutoff of 3 kDa (Merck Millipore). A 10 µL aliquot of the concentrated exoproteome fraction was reduced with 5 mM tris (2-carboxyethyl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were ruptured by sonication and the suspension was centrifuged at 17,100 × g for 1 h in an Eppendorf 5430R centrifuge and the cleared supernatant was further filtered using a 0.22 µm syringe filter (Merck Millipore). Using an imidazole gradient for protein elution ...
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bioRxiv - Cell Biology 2023Quote: ... Conditioned media were collected after 48 h and centrifuged at 3,000 g for 10 minutes before passing through a 0.22 μm Millex® syringe filters (Merck Millipore). For viral infection ...
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bioRxiv - Immunology 2023Quote: ... The tetramer (428 kDa) was purified from excess GFP-biotin (25 kDa) with a 100 kDa cut-off Amicon Ultra centrifugal filter (Merck). Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution ...
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bioRxiv - Microbiology 2023Quote: ... we saturated the porous medium with a florescent dye (fluorescein sodium salt, from Merck, visible through a GFP filter cube) and recorded a large image of the entire structure ...
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bioRxiv - Microbiology 2023Quote: ... These samples were filtered onto 5.0 µm pore-size polycarbonate filters (diameter, 25 mm; Isopore, Sigma-Aldrich, Merck, Darmstadt, Germany) using a filter holder (1225 Sampling Manifold Chambers ...
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Immunology 2023Quote: 200 μg of HLA.A2-peptide complex in PBS was concentrated to 1 mg ml−1 with the use of Amicon Ultra Centrifugal Filters (Merck), adjusted to pH 8.3 by adding freshly prepared NaHCO3 (0.1 M final concentration ...
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bioRxiv - Microbiology 2023Quote: ... The volume of concentrated viral fractions (>50 kDa) was further reduced using Amicon Ultra 15 ml Centrifugal Filters (Merck-Millipore) prior to phenol/chloroform extraction ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was added to Fe-NTA agarose beads (PureCube) on a multiscreenHTS-HV 0.45 μm 96 well filter plate (Merck Millipore). After loading ...
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bioRxiv - Immunology 2023Quote: ... Both the native fab and the deglycosylated fab were buffer exchanged into 150 mM ammonium acetate (pH 7.5) using Amicon 10 kDa MWCO centrifugal filters (Merck Millipore). The samples were loaded into gold-coated borosilicate capillaries (in-house prepared ...
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bioRxiv - Biophysics 2023Quote: ... and was subsequently concentrated to 1 mL using a 100 kDa cut-off Amicon ultra centrifugal filter (Merck Millipore, Germany). The concentrated protein sample was further purified by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2023Quote: ... The next day the proteins were collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore) for size exclusion chromatography.