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Citations for Merck :
1551 - 1600 of 1811 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... structured microcosms were set-up in multi-well plates with a 0.22 µm filter membrane at the bottom of the well (Merck, Darmstadt, Germany) and containing 250 mg of sterile glass beads (with diameter of 80 to 120 µm ...
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bioRxiv - Molecular Biology 2021Quote: ... concentrated to 33 mg/ml using an Amicon Ultra 50 ml 10 kDa molecular weight cut-off centrifugal filter (Merck Millipore) and then aliquoted before flash freezing the protein sample in liquid nitrogen ...
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bioRxiv - Microbiology 2020Quote: ... and finally over 0.22 µm pre-cleaned44 hydrophilic PVDF filters (<600 mbar under-pressure; 47 mm, Durapore, Merck Millipore, Cork, Ireland). Filtration led to 70-84% reduction in the abundance of large virus-like particles (VLP ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant MBP fusion protein was concentrated with an Amicon Ultra-15 centrifugal filter unit (Ultracel 3K; Merck Millipore, Darmstadt, Germany) and used directly for electrophoretic mobility shift assay (EMSA).
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bioRxiv - Cell Biology 2020Quote: ... from affinity purification were pooled and buffer exchanged using Amicon™ Ultra-15 Centrifugal Filter Unit (10 kDa cutoff; Merck™) in 10 mM HEPES-NaOH buffer ...
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bioRxiv - Immunology 2020Quote: ... samples were centrifuged at 22000 x g for 20 minutes at 4 °C and the supernatant was filtered through Millex GV 220 nm filter (Merck-Millipore). For acid treatment acetic acid was used with 0.1 N final concentration ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Microbiology 2021Quote: ... to remove larger organisms and was filtered on board first through 5 μm and then through 0.2 μm polycarbonate filters (47 mm, Merck, Darmstadt, Germany) using pressurized air ...
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bioRxiv - Synthetic Biology 2021Quote: ... 500 μL of the perchlorate extract was filtered with a 30K centrifugal filter (Amicon Ultra - 0.5mL, Ultracel - 30K, UFC503096, Merck KGaA, Germany), at 14000 g ...
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bioRxiv - Systems Biology 2021Quote: ... A volume of 1 mL supernatant was transferred to an LC glass vial using Millex-HV PVDF syringe filter tips (Merck Millipore). The HPLC column (Aminex 300-mm HPX-87H ...
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bioRxiv - Microbiology 2021Quote: ... and the remaining sample was centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2021Quote: ... they were centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2020Quote: ... Purified DesX was desalted and concentrated by centrifugal filtration using an Amicon Ultra centrifugal filter unit (10 kDa cutoff; Merck Millipore). Before the assay ...
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bioRxiv - Cell Biology 2021Quote: ... method [68] with the following modification: Equal volumes of spent media were concentrated to approximately 50 μL with Microcon - 10 kDa centrifugal filter units (Merck Millipore) at a speed of 14,000 x g ...
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bioRxiv - Immunology 2021Quote: ... After digestion for 2 min in a water ice bath the samples were separated by centrifugation at 1000 x g for 30 s at 0°C using a 22 µm filter (Merck, Millipore) and were immediately analyzed by LC-MS ...
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bioRxiv - Immunology 2022Quote: ... Supernatant from doxycycline-induced or uninduced cells (40mL per condition) was collected and concentrated to 500 μL using Amicon Ultra-15 filters with 10 kDa cutoff (Merck Millipore). The concentrated samples were loaded on a Superose 6 10/300GL column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently visualized by Coomassie-stained SDS-PAGE before being concentrated using a 10 kDa MWCO Amicon Ultra centrifugal filter (Merck Millipore) to appropriate concentrations for further analysis.
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of the affinity purification was concentrated to a volume between 1-2 mL before injection using centrifugal filter units (Amicon, Merck millipore) with a MWCO of 5,000 Da ...
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bioRxiv - Cell Biology 2022Quote: ... The selected elution fractions (1-3 of 500 μL each) were pooled and subsequently concentrated using 100-kDa Amicon centrifugal filter units (Merck Millipore). The concentrated samples were subjected to several washing steps with PBS to obtain a highly pure EV population ...
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bioRxiv - Biochemistry 2022Quote: ... The cleaved protein was loaded on a 5 mL Ni-Sepharose cartridge and the flow-through was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified protein was dialyzed overnight in 25 mM Tris-Cl pH 7.5 using SnakeSkin®ialysis tubing (7 kDa molecular weight cutoff) and concentrated using Amicon™ Ultra-15 Centrifugal Filter Units (30 kDa molecular weight cutoff) (Merck) according to the requirements of the experiments.
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Biochemistry 2022Quote: Collected samples from the first main peak were concentrated by ultrafiltration on 30K Amicon® Ultra-0.5 Centrifugal Filters Units (Merck Millipore) in a fixed rotor 14 000 g ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved His-tagged GB1 and remaining TEV were removed via a second Ni-NTA purification after buffer exchange to Buffer A using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S200 Increase 26/600 column equilibrated in HEPES buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... 2 mL of EV-containing eluate was collected and concentrated to 100 μL using Amicon Ultra-2 10K filters (Merck Millipore).
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bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... pre- equilibrated with PBS) and were concentrated to 1 mg/mL using a 10-kDa cutoff Amicon Ultracell Centrifuge Filter Unit (Merck, UFC5010BK), and stored at 4°C in the dark.
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bioRxiv - Microbiology 2022Quote: ... Resultant elution fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff; Merck Millipore), and the enzyme preparations were stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mM NaCl) using a 3 kDa (or 10 kDa) molecular weight cut-off (MWCO) Amicon Ultra Centrifugal Filter Unit (Merck Millipore), and used directly or aliquoted ...
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bioRxiv - Neuroscience 2022Quote: ... Virus particles collected from the cell lysate and supernatant were purified by ultracentrifugation with OptiPrep (1114542, Axis-Shield) and concentrated by ultrafiltration with Amicon Ultra-15 Centrifugal Filter Unit (UFC903024, Merck Millipore). The physical titer of the virus vector (genome copies (gc)/mL ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free supernatant (CFS) was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... and concentrated to 2 mg chlorophyll mL-1 using a 100-kDa molecular-weight cut-off centrifugal filter unit (Amicon Ultra-15, Merck Millipore). A 3 µl volume of the sample was applied to a glow-discharged holey carbon grid (GIG ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing BirA were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Molecular Biology 2022Quote: ... the heart was removed and cells were dissociated and filtered through a 70 µm filter after which BSA (Merck Life Science) was added to a final concentration of 4% to inactivate the enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing CLPB3 were pooled and concentrated in Amicon® Ultra-4 Centrifugal Filter Units (Ultracel®-3K, Merck Millipore Ltd), with a subsequent buffer exchange to 6 M Urea ...
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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bioRxiv - Microbiology 2024Quote: ... Supplements were prepared as 50 mM stock solutions in Milli-Q water and sterilized by filtration with syringe filters (Millex-GV, 0.22 µm, PVDF, Merck, Darmstadt, Germany). Each inoculated main culture was divided into three or four different wells (biological replicates ...
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bioRxiv - Microbiology 2024Quote: ... This suspension was diluted with 9 mL of 0.9 % NaCl and filtered through a hydrophilic polycarbonate filter (pore size 0.2 µm; Merck Millipore, Darmstadt, Germany). A segment from the center of the filter was fixed with 1 % glutaraldehyde and 4 % paraformaldehyde in washing buffer (10 mM cacodylate buffer ...
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bioRxiv - Microbiology 2024Quote: ... All but one sample for microbiological analyses was collected onto 0.22 μm pore size filters (type GVWP; Merck Millipore, Darmstadt, Germany) held in pressure-resistant stainless steel filter holders directly connected to the tubing outlet under in-situ hydraulic pressure conditions ...
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bioRxiv - Bioengineering 2023Quote: ... and concentrated to a final volume of 120 μl using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA). For plasma volume higher than 500 μl ...
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bioRxiv - Biophysics 2024Quote: ... nsp16 and nsp7-11 were exchanged by six rounds of dilution and concentration in centrifugal filter units (Amicon Ultra, 10 K MWCO, Merck Millipore).
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...