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Citations for Merck :
1301 - 1350 of 1811 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the supernatant (termed here EV-free SN) was saved and concentrated by Amicon Ultra-0.5 centrifugal filters (Merck Millipore). The pelleted EVs were washed in cold ...
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bioRxiv - Biophysics 2022Quote: ... Elution fractions with Casπ were pooled together and concentrated using 30kD molecular weight cut-off centrifugal filters (Merck Millipore), and further purified by size exclusion chromatography column (Superdex 200 Increase 10/300 ...
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bioRxiv - Genomics 2023Quote: ... DBCO-PEG4-conjugated antibodies were concentrated using an Amicon Ultra-0.5 NMWL 10-kDa centrifugal filter (Merck Milipore, UFC501024) and measured on a NanoDrop™ 2000 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Plant Biology 2023Quote: ... Purified proteins were concentrated to 10–30 mg/ml by using a 10-kDa Amicon centrifugal filter device (Merck), flash-frozen in liquid nitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 L of the water was filtered using a Sterivex filter cartridge with a nominal pore size of 0.45 μm (SVHV010RS, Merck) and a pump (Aqua Loader AL898 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was concentrated to 10% of the initial volume using a 100 kDa cutoff Amicon filter (Merck Millipore).
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bioRxiv - Microbiology 2024Quote: ... coli SM10 pCVD442-up-dotB-down and the SP-1303 ΔdotB mutant by mixing equivalent volumes of adjusted overnight (O/N) liquid culture to OD=2 on a 0.45 µm filter (peel corner, MERCK) positioned on a LB agar plate for 6 h at 28°C ...
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bioRxiv - Immunology 2024Quote: ... followed by centrifugation at 1,000 rpm for 5 min and filtration using 0.45 μm sterile syringe filters (Merck Millipore). The filtrate was then concentrated with a 100 kD Amicon ultra centrifugal filter unit (Merck Millipore) ...
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bioRxiv - Genetics 2024Quote: ... The harvested medium was then centrifuged at 1,300 rpm at 4 °C for 5 min to remove cells and then filtered by 0.45 μm filter (Merck) and the virus was collected and stored at -80 °C.
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bioRxiv - Molecular Biology 2024Quote: ... 200 µg/sample or the complete fraction were filtered using an Amicon Ultra Centrifugation filter 50 kDa MWCO (Merck). The material retained on the filter was resuspended in 300 µl SEC-Buffer (1 M NaCl ...
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bioRxiv - Genomics 2024Quote: ... ratio by a Gibson approach with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
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bioRxiv - Biochemistry 2024Quote: ... Purified rAAVs were concentrated using Amicon® Ultra 15 mL 100 kDa MWCO Centrifugal Filters (Merck KGaA, Darmstadt, Germany) and the buffer was exchanged to PBS pH 7.3.
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bioRxiv - Molecular Biology 2024Quote: ... The peak fractions were pooled and concentrated using centrifugal filters with a 10 kDa molecular weight cut-off (Merck). Finally ...
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bioRxiv - Immunology 2024Quote: ... eggs were pelleted by centrifugation at 400 x g (5 minutes) and HES supernatant passed through a 0.22 μm syringe filter (Merck) and stored at -80°C.
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bioRxiv - Immunology 2024Quote: ... The resulting LNP formulation was concentrated using an Amicon Ultra 50 K MWCO centrifugal filter device (Merck Millipore, UFC905096) and subsequently passed through a 0.22-μm syringe filter (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... ADPG2 and PGLR proteins were concentrated using an Amicon Ultra Centrifugal filter with a 10 kDa cut-off (Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... The lumen proteins were concentrated at 14,000 rpm for 10 min at 4 °C using Amicon ultra-0.5 centrifugal filters (Cat. No. UFC500324, Merck millipore). All solutions contained protease inhibitors with final concentration of 1 mM benzamidine ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing TEV protease were concentrated and buffer exchanged using Amicon Ultra-15 centrifugal filters (10 kDa MWCO, Merck) into 40 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2024Quote: ... for 4 h at 4°C and then concentrated to 200 ng/μl using Amicon Ultra-0.5 centrifugal filter units (Merck). Nucleosomes are then diluted with ice-cold 0 M FRET buffer to a concentration of 50 nM ...
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bioRxiv - Genomics 2024Quote: ... and ligated by Gibson with 3 h of incubation at 50°C followed by dialysis for 3 h on a membrane filter (MF-Millipore 0.025 μm membrane, Merck) and vacuum concentration ...
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bioRxiv - Biochemistry 2024Quote: ... cell culture supernatant was harvested by centrifugation at 3000 x g for 10 min and then filtered using 0.45 µM pore size filters (Merck). The supernatant was supplemented with 10 mM imidazole prior to purification using a HisTrap HP ...
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bioRxiv - Biophysics 2024Quote: ... The resulting product was then dialysed for 3h by using a membrane filter (MF-Millipore 0.025 µm membrane, Merck) and concentrated 10X using a speed vacuum concentrator ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing CODANIN-1_ASF1A_CDIN1 fractions were collected and concentrated by Amicon Ultra Centrifugal Filter 100 kDa MWCO (Merck Millipore) up to 3mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through fraction was then concentrated using an Amicon Ultra filter (Merck, molecular weight cut-off 10 kDa), and was further purified by size-exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions were collected and concentrated to 22.0 mg/mL using a 100 kDa MWCO Amicon centrifugal filter (Merck Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... CM was separated into three fractions by three centrifugal filter devices (3 kDa, 10kDa and 30 kDa) (Merck Millipore). 10% CM ...
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bioRxiv - Cell Biology 2024Quote: ... Glyc-rHDL and Ox-rHDL were filtered with a 0.22μm low protein-binding low-retention filter (Merck Millipore, Ltd.). Protein concentrations of apoA-I ...
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bioRxiv - Cell Biology 2024Quote: ... Fabs were concentrated up to more than 1 mg/mL using 10 k cut-off filters (Amicon Ultra-0.5 10 k; Merck), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Biophysics 2021Quote: ... The His-tag cleaved Mpro in the flow-through was buffer-exchanged to 20mM Tris pH 8 using Amicon Ultra centrifugal filter (MWCO. 10kDa, Merck) and then loaded onto 5mL Q Sepharose column (HiTrap Q HP ...
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bioRxiv - Biophysics 2021Quote: ... The solution was concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, MA, USA) and loaded into a Superdex75 10/300 GL gel filtration column (GE Health-care Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... Viruses were collected from the supernatant 24 hours later and cleared by centrifugation at 2000 rpm for 3 minutes followed by filtration with 0.45 µm PVDF syringe filter units (Merck, #SLHV033RS). Cleared supernatants were titrated by plaque assay using BHK-21 cells.
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bioRxiv - Molecular Biology 2021Quote: ... The sample (0.5-1 ml) was transferred to Amicon Ultra 0.5 ml Centrifugal Filters (MWCO=30 kDa, Merck, cat# UFC503096) and further concentrated to a final volume of 200 μl.
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bioRxiv - Neuroscience 2021Quote: ... 0.1 M Tris pH 8.5) and processed according to a CHAPS-based FASP protocol in centrifugal filter units (30 kDa MWCO, Merck Millipore). After removal of the detergents ...
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bioRxiv - Molecular Biology 2021Quote: ... The 40S- and 60S-subunit fractions were mixed and concentrated using Amicon Ultra filter units (MWCO 50 kDa, Merck Millipore). The concentrated mixture was loaded onto a 10–50% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Biophysics 2020Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with 10kDa Amicon®Ultra-4 centrifugal filters (MERCK). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... The resulting 5.6-mL fraction was concentrated to 250 μL by ultrafiltration using Amicon Ultra-4 10K centrifugal filters (Merck, USA), then submitted to immunodetection.
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bioRxiv - Biochemistry 2021Quote: ... 100 mM EDTA or 200 mM Imidazole) and concentrated up to 0.5 ml using Amicon Ultra-15 centrifugal filters with MWCO of 50 kDa (Merck, Germany). The sample was then applied to a size exclusion chromatography on a Superdex 200 increase 10 /300GL column equilibrated with wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Cell culture supernatants were collected after 48 hr and 72 hr and filtered through 0.22 µm syringe filters (Merck Millipore). Filtered supernatants were stored at -80 °C until further analysis ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... supernatant collected at 240 hrs was filtered with 0.45 µm and 0.2 µm low-binding protein Durapore® filters (Merck Millipore). 25 µL of clarified supernatant was directly injected on a Vanquish™ uHPLC (Thermo Sceintific ...
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bioRxiv - Bioengineering 2020Quote: ... The solution was removed from the centrifuge and filtered through a syringe filter with a 25 mm diameter (Merck Millipore) into a clean syringe at least twice to ensure no precipitates were present ...
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bioRxiv - Biochemistry 2020Quote: ... Strep-GlnA1 was eluted in the presence of 2.5 mM D-Desthiobiotin (IBA, Göttingen, Germany) followed by buffer exchange using an amicon centrifugal filter (30KDa) (Merck; Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Protein pellets were dissolved in 50 μl of 8 M Urea and loaded onto a 30 K centrifugal filter unit (Merck). Preparation for MS was done as described in [64] ...