Labshake search
Citations for Merck :
5651 - 5700 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: DDX5 KO HCT116 cells were transduced in 6-well plates using the lentiviral supernatant diluted in fresh medium supplemented with 4μg/mL of polybrene (Merck, Sigma-Aldrich). The medium was changed after 8 h and the selection with the appropriate antibiotic was initiated after 48 h ...
-
bioRxiv - Microbiology 2024Quote: ... This suspension was diluted with 9 mL of 0.9 % NaCl and filtered through a hydrophilic polycarbonate filter (pore size 0.2 µm; Merck Millipore, Darmstadt, Germany). A segment from the center of the filter was fixed with 1 % glutaraldehyde and 4 % paraformaldehyde in washing buffer (10 mM cacodylate buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... the supernatants were exchanged three times to the same buffer as above (Amicon Ultra 0.5 mL, 10 kDa cut off (Merck, Darmstadt, Germany) (10,000 g for 20 min at 4 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.5 ml nuclei wash, and suspension buffer (NWS-Buffer: 2% BSA, 2 mM MgCl2, 0.5% Protector RNAse Inhibitor (SKU3335399001, Merck, Soeborg, Denmark)) was carefully added ...
-
bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Complex containing fractions were pooled and concentrated again using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
-
bioRxiv - Microbiology 2023Quote: ... the stabilized pellets were enzymatically lysed for 30 min in a lysozyme solution (15 mg/ml in TE buffer; L4919 Sigma-Aldrich, Merck) combined with 1:10 (v/v ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein sample obtained after Ni-NTA was concentrated up to 5 ml using a centrifugal filter unit (Merck & Co., USA) with a cutoff size of 10 kDa ...
-
bioRxiv - Microbiology 2023Quote: ... 10 pylorus and ileum sections from bees coming from the same cage were pooled in a 2 ml screw cap tube containing 750 μl of TRI reagent (Sigma-Aldrich, Merck), glass beads (0.75-1 mm diameter ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were grown until confluence in 0.2 mg/ml collagen-type I-coated 6 well plates (Merck, Darmstadt, Germany) and incubated in serum-free medium for 24 h with/without Dox treatment ...
-
bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
-
bioRxiv - Developmental Biology 2023Quote: ... feeder depleted mESCs were seeded for EB formation at 25,000 cells/ml in ESGROTM Complete Basal Medium (ESGRO, Merck, SF002-500) in non-adhesive 60 mm or 100 mm culture dishes ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was removed (around 25 ml) and either concentrated 10-fold with Amicon 3 kDa spin columns (Merck, cat. #UFC900308) and brought to a final volume of 2 ml for subsequent Western blot analysis (Fig.1B ...
-
bioRxiv - Microbiology 2023Quote: ... the supernatant from late-log cultures was concentrated to <100 μL and washed (with 400 μL of ddH2O) in 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) using centrifugation at 5000 g ...
-
bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... for 15 min at RT and stained with filipin (50 μg/mL in PBS prepared freshly from a 250-fold ethanolic stock; #F9765, Sigma-Aldrich/Merck) for 2h at RT in the dark ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... were homogenized (60 Hz, 90 s) and extracted by ethanol three times (95%, 3 × 300 mL, vortex 10 min each, Merck, Germany). The three batches of extracts were pooled together and centrifuged for 20 min at 10000g and 4°C (Bruker ...
-
bioRxiv - Developmental Biology 2023Quote: ... female mice aged 6-8 weeks were stimulated to ovulate by 10 IU intraperitoneal injection of Foligon/PMSG (MSD Animal Health, Spain) followed by intraperitoneal administration of 10 IU of Ovitrelle 250 µg/0.5 mL (Merck Serono, Germany) 48 h later ...
-
bioRxiv - Biochemistry 2024Quote: ... Cell pellets were then washed in cold 50 mL cold PBS cOmplete™ EDTA-free Protease Inhibitor Cocktail tablets (Merck, #73567200) and centrifuged at 2000 rpm for a further 10 minutes ...
-
bioRxiv - Bioengineering 2024Quote: ... the eluate was reduced to approximately 40–50 ml and then concentrated using an Amicon Ultra-15 10 kDa molecular mass cut-off spin filter (Merck Millipore). Final EV preparations were kept in sterile filtered PBS for immediate use or sterile PBS-HAT buffer56 for storage at −80℃.
-
bioRxiv - Cancer Biology 2024Quote: ... and lysed on ice for 30 min at a cell density of 107 cells per ml of RIPA lysis buffer (Merck Millipore), supplemented with phosphatase and complete protease inhibitors (Merck Roche) ...
-
bioRxiv - Microbiology 2024Quote: Asexual spores of the strain HEK1 were collected from a fully colonized plate (10 cm diameter) with 15 mL of Tween® 80 (0.016%) (Merck, DE). The resulting suspension was centrifugated (Sigma 2-16PK ...
-
bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was diluted in 10 ml of 1X PBS buffer and filtered using an Amicon 50 kDa filter column (Merck, Darmstadt, Germany) to concentrate the extract to a final volume of 500 μL.
-
bioRxiv - Microbiology 2019Quote: ... 1 mL of the pre-enriched suspensions for each drinking water sample was added to sterile tubes containing 9 mL of TTB (Merck, South Africa). The suspensions were subjected to incubation at 36 ± 1 °C overnight ...
-
bioRxiv - Biochemistry 2021Quote: ... Pellets were thawn on ice and resuspended in lysis buffer (5 mL/g pellet: 50 mM Na2HPO4/KH2PO4, 150 mM NaCl, 0.2 % Tergitol™ solution (Merck, Darmstadt, Germany), and 2 mM DTT ...
-
bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
-
bioRxiv - Bioengineering 2021Quote: Inflammatory stimulations were performed by treating the chips for 24 h from d4-d5 or d11-d12 with TNF-α (final concentration of 20 ng/mL; SRP3177; Merck KGaA) or LPS (final concentration of 100 ng/mL ...
-
bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
-
bioRxiv - Microbiology 2021Quote: ... The viral particles were suspended in 10 ml phosphate-buffered saline (PBS) and filtered using a 0.45-μm filter (Millex-AA, Merck Millipore, Darmstadt, Germany). The filtered viral particles were centrifuged (8,500×g ...
-
bioRxiv - Cell Biology 2021Quote: ... up to 15 ml filtered supernatants were ultra-filtered through a 3 kDa MWCO Amicon® Ultra device (Merck-Millipore, MA, USA) by centrifugation at 4000 x g for 1 h at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... coli transformed with plasmid pET42 expressing N-terminally GST- and HIS-tagged human IL-18 were grown at 37°C in Lysogeny broth (LB) medium containing kanamycin (25 μg/ml) as selection marker for the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium). When the optical density at 600 nm (OD600 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... The total extract of proteins was then centrifuged for 20 min at 20,000 g and the soluble fraction loaded on an affinity chromatography with 2 ml of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). The resin was washed with 20 ml of 20 mmol/L Tris-HCl (pH7.9 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Polar metabolites were derivatized for 90 min at 37 °C with 20 μl of 20 mg per ml methoxyamine (Merck Sigma, 226904) in pyridine (Merck Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... The TEV cleaved complex was concentrated to around 500 μl using a 4 ml Amicon® Ultra centrifugal filter concentrator (Merck Millipore) with a membrane molecular weight cut off 10 kDa ...
-
bioRxiv - Immunology 2022Quote: ... at pH 2.0 with final protein concentration of 1.75 mg/mL (Sphβ preparation) or in 40% ethanol (v/v from ethanol absolute, Merck KGaA, Darmstadt, Germany), 0.25 M NaCl at pH 1.8 with final protein concentration of 5 mg/mL (Sphα preparation) ...
-
bioRxiv - Microbiology 2022Quote: ... 0.5 ml of each aliquoted sample was filtered onto a 25 mm polycarbonate filter (pore size: 0.45 µm, Merck KGaA, Darmstadt, Germany). The filters were then air dried under sterile conditions and mounted onto 25mm diameter aluminum pin stubs (Agar Scientific Ltd. ...
-
bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
-
bioRxiv - Genomics 2024Quote: ... 500 mL vent-water was passed through a sterile 0.22 μm mixed cellulose ester membrane filter (Merck Life Science Private Limited, India) having a diameter of 47 mm ...
-
bioRxiv - Neuroscience 2024Quote: ... Postoperative care included antibiotic and analgesic administration (2.27% enrofloxacin, 5 mg/kg, Bayer, Kiel, Germany and 0.3 mg/ml buprenorphine, 8 µg/kg, Buprex, Merck & Co., Inc., NJ, USA) in a controlled temperature environment (37°C) ...
-
bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
-
bioRxiv - Microbiology 2024Quote: ... The flow-through was further concentrated from 10 ml volume in an Amicon® Ultra-4 Centrifugal Filter 50 kDa unit (Merck Millipore) and stored at 4 °C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... A small aliquot of each sample was added to Hemi medium17 with a 5-μl/ml antibiotic cocktail (P4083, Merck, Darmstadt, Germany) and incubated under dark conditions at 19–20 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were eluted using IMAC elution buffer (IMAC buffer A + 300 mM IMD) and concentrated using Amicon Ultra – 0.5 mL units (50 kDa MWCO, Merck Millipore, Darmstadt, Germany). SEC was performed as described above ...
-
bioRxiv - Microbiology 2023Quote: ... proteins were ultracentrifuged at 13000 rpm for 10 min at 4°C and concentrated to 1.5 ml using centrifugal filters (Merck, Amicon Ultra-4). They were then exchanged into buffer #5 overnight at 4°C and aliquoted and stored at -80°C ...
-
bioRxiv - Neuroscience 2023Quote: Blood was collected in 2 ml EDTA tubes that were prepared with 100µl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany). These tubes were centrifuged immediately after venepuncture for 15 min at 4⁰C and 3200g ...
-
bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were concentrated to a volume of approximately 2 mL using Amicon® Ultra centrifugal filters with a NMWC of 10 kDa (cat# UFC801024, Merck Millipore).