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Citations for Merck :
5401 - 5450 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... E12.5 whole-mount embryos were analysed for β-gal activity with X-gal (0.6 mg/ml; Merck Millipore, B4252) in PBS buffer containing 4 mM potassium ferricyanide ...
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bioRxiv - Molecular Biology 2019Quote: ... from an 18500g centrifugation (6 min at +4°C) were run through 3-kDa cut-off centrifugal filters (Amicon Ultra-0.5 ml, Merck) into pre-weighted collection tubes to remove remaining macromolecules ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 1.5 mL cultures by using the GenElute™ Bacterial Genomic DNA Kit (Merck, Germany). Linker PCR was used to test individual transformant colonies and to determine the diversity of insertion sites ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Microbiology 2019Quote: ... IFNβ production was stimulated by transfecting cells with (10 μg/mL) polyinosinic: polycytidylic acid (poly I:C) (Sigma Aldrich, Merck). Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Growth assays on solid medium were done as described (Schuck et al., 2009) using SCD plates with and without 0.2 µg/ml tunicamycin (Merck). Plates were imaged after 1.5 days of growth at 30°C.
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bioRxiv - Biochemistry 2022Quote: The protein stock solutions were transferred to a deuterated buffer containing 35 mM potassium phosphate and 25 mM NaCl in D2O at a pD of 7.5 using 0.5 mL Centricon devices (Merck Millipore) with a MWCO of 30 kDa by washing and centrifuging several times at 10,000 x g and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES pH 7.5, 400 mM NaCl, 20 mM imidazole, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Immunology 2022Quote: NPC cellular phenotypes were induced in RAW 264.7 and J774.A MΦ by treating with 2 μg/ml U18666A (Merck) for 30h or 72h respectively ...
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bioRxiv - Cell Biology 2022Quote: ... the nuclei were visualized with Hoechst 33258 (Merck, B2883, 2 µg/ml in H2O at RT for 5 min).
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bioRxiv - Biochemistry 2022Quote: ... The mixture was concentrated using an Amicon Ultra 0.5 ml filter unit (50 kDa molecular weight cut off, Merck). A final concentration of the complex was approximately 11.2 µM ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Pathology 2022Quote: ... Nose and throat swabs were collected in tubes containing viral transport medium (15% sucrose, 2.5 μg/ml Amphotericin B [Merck] ...
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bioRxiv - Microbiology 2020Quote: A 100μL bacterial suspension of 2.0 × 106 cells/mL was prepared and loaded on the four-chambered CellASIC ONIX B04A-03 microfluidic platform (Merck). Cells were trapped with the following pressure and flow time settings ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was applied to a centrifugation filter (Amicon Ultra 0.5 mL 3 K device; Merck KGaA, Darmstadt, Germany), and the fraction containing free amino acids was obtained by centrifugation (15,000 × g ...
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bioRxiv - Microbiology 2019Quote: ... An inoculum from the SDA plate was suspended in 25 ml of Sabouraud dextrose broth (SDB; Merck, Darmstadt, Germany) and incubated for 18 h under agitation (120 rev/min ...
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bioRxiv - Microbiology 2021Quote: ... The diced tissue was then digested for 60 minutes at 37°C in 12ml of a pre-heated suspension of collagenase I (2.4mg/ml, Merck) and DNase I (1mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biophysics 2021Quote: ... the dry lipid film was rehydrated with 3 mL of 0.2 M 8.5 pH Bicine (Merck Life Science, Norway) buffer (pH adjusted with NaOH (Merck Life Science ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Water samples (500 mL) from each bottle were filtered and collected using a Sterivex filter (0.45 µm pore size; Merck Millipore ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We collected and filtered 500 mL of the water from each bottle using 0.45-µm Sterivex filters (Merck Millipore) at 0 ...
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bioRxiv - Immunology 2020Quote: ... with 1μg/mL anti-CD3 and 1μg/mL anti-CD28 at 4°C at a density of 105 cells/m in starvation media (RPMI (Merck), containing 0.5% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... USA) at a multiplicity of infection (MOI) of 5–10 in 1.5 mL Ex-Cell 405 medium (Merck, Germany), and the infected cells were incubated at 26 °C ...
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bioRxiv - Immunology 2020Quote: ... conditional knock out) allows deletion of Csf1r following treatment with the tetracycline analog doxycyclinehyclate (2μg/ml in 5% sucrose water; Merck) causing CSF1R expressing macrophage populations to be depleted ...
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bioRxiv - Immunology 2021Quote: ... Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck), respectively ...
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bioRxiv - Microbiology 2020Quote: ... live cell fluorescence microscopy was performed as described before (85) with minor modification using 5 μg/ml Hoechst (Merck) to stain the nuclei ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 4 mL of the culture were vacuum-filtrated using 0.45 μm pore size filters (HVLP02500, Merck Millipore). The filters were transferred to -20°C cold 40:40:20 acetonitrile:methanol:water for metabolite extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Microbiology 2022Quote: ... we filtered three aliquots of 1000 ml per water sample through 0.45μm 47mm white gridded mixed ester cellulose membranes (Merck, USA). Membranes were placed on 55 mm Baird-Parker with potassium tellurite enrichment agar plates and incubated at 37°C for 48 hours ...
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bioRxiv - Molecular Biology 2022Quote: All samples were centrifuged at 16,000 × g for 30 min and 400 µL supernatant was loaded on a 0.5 mL centrifugal ultrafiltration unit with 3 kDa cut-off membrane (Amicon, Merck) and concentrated down to 20 µL ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...
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bioRxiv - Plant Biology 2023Quote: ... dried and transferred in a grid pattern onto square plates containing 50 ml of MS/2 medium (0.5× Murashige and Skoog salts [Merck Sigma-Aldrich] ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we re-analyzed microarray-based transcriptome datasets: i) HepG2 cells treated with vehicle (0.0125% DMSO) or 12 μg/ml silymarin (Merck) for 24 h (n = 3 biological replicates)98 ...
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bioRxiv - Neuroscience 2023Quote: ... Patch electrodes were filled with KCl-K-gluconate-based internal solution with gramicidin (20 μg/mL, G5002, Merck KGaA), 5-CFDA ...
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bioRxiv - Molecular Biology 2024Quote: ... Thorough vortexing and centrifugation at 11,000 rcf for 5 min was repeated and the upper water phase containing DNA was carefully transferred to a microfuge tube with 0.5 mL H20 saturated n-butanol (Merck). Tubes were vortexed and centrifuged at 11,000 rcf for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with lentiviral particles in the presence of polybrene (8 g/mL, Cat# TR-1003-G, Merck); after 48 hours of transduction ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli were determined by dilution plating on LB + 0.5 % agar and LB + 0.5 % agar with Kanamycin (40 μg / mL) (Merck Germany).
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bioRxiv - Evolutionary Biology 2024Quote: ... coli were determined by dilution plating on LB + 0.5 % agar and LB+0.5% agar with Kanamycin (40 μg/mL) (Merck Germany).
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bioRxiv - Genomics 2024Quote: ... media was changed for 500 µL of DMEM +5% FBS +4 μg/mL of polybrene (Merck TR-1003-G). Lentiviruses were diluted to the desired MOI in 500 µL of DMEM +5% FBS and slowly added to each well ...
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bioRxiv - Genomics 2024Quote: ... Cells were then resuspended in 100µL RPMI + 10% FCS and stimulated for 4h with PMA (100ng/mL, Merck, P8139) and ionomycin (1µg/mL ...
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bioRxiv - Immunology 2024Quote: ... Spleens were minced into fine pieces using forceps and digested by incubation with 1mg/ml collagenase (Sigma-Aldrich/Merck) and 30 units/ml DNase (Roche ...