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Citations for Merck :
5601 - 5650 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... containing labeled or unlabeled glucose/glutamine and quenched by adding 50% ice-cold methanol containing 2 µg/mL cinnamic acid (Merck) as an internal control ...
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bioRxiv - Synthetic Biology 2024Quote: ... Stimulation of the controls was performed 8 h after transfection with 20 ng/ml TNF-α (Merck, cat. no.: H8916). Cells were lysed 24 h later by addition of 100 μl lysis buffer (25 mM Tris/HCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Seed cultures from glycerol stocks were inoculated 20 hours overnight in 5 mL Invitrogen Luria Broth Base LB medium (ThermoFischer, USA) and supplemented with Kanamycin sulfate (Merck, Germany) (50 μg/mL) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... ∼105 log-phase cells from 200 ml mass culture were concentrated on hydrophilic nylon membranes (Nylon-Netzfilter, 10 µm pore size, 47 mm, Merck KGaA), rinsed in mineral water (Volvic® ...
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bioRxiv - Developmental Biology 2021Quote: ... fully grown oocytes at the GV stage were collected from large antral follicles and released into M2 medium supplemented with 150 μg/ml dibutyryl cyclic (dbc) AMP (Merck KGaA). After being freed from cumulus cells by pipetting ...
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bioRxiv - Microbiology 2019Quote: C6/36 or Huh7 were seeded in 8-well chambers and confluent monolayers were incubated with 150 or 300 μg/mL of HDL (Merck, USA) in specific growth medium without FBS for 1.5 h ...
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bioRxiv - Cell Biology 2022Quote: ... Growth medium was then removed from apical side and on the basolateral side it was replaced by ALI differentiation medium +/- 10 ng/ml IL-13 (IL012; Merck Millipore). Once the ALI interface was established medium was exchanged every second day till day 25-28 on ALI ...
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bioRxiv - Developmental Biology 2022Quote: ... Organoid cells were cultured in self-renewing medium with ROCKi for 8 days before Dox (Doxycycline hyclate, 2 µg/ml, Merck, D9891) and TMP (Trimethoprim ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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bioRxiv - Microbiology 2019Quote: ... TX 3211 Alpha Wipe, ITW Texwipe, Kernersville, US, 23×23 cm; 20 ml autoclaved ultra-pure water for chromatography, LiChrosolv, Merck Millipore). Three sampling sessions were performed ...
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bioRxiv - Plant Biology 2019Quote: ... resuspended in 5 ml 10 mM Sodium-acetate (pH 5.0) and concentrated with Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore, 10K) ∼10 fold ...
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bioRxiv - Plant Biology 2019Quote: ... Pelet was dissolved in 2 ml DDW and passed through a 0.2-μm membrane filter (Millex-GV Filter Unit, Merck Millipore, Ireland). The filtrate was used for sucrose ...
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bioRxiv - Biochemistry 2020Quote: ... the original 8 μM 655-QDs suspension in borate buffer (50 mM borate) from the supplier was pipetted into 1.5 mL fresh ul-trapure water (Merck Millipore, US) of varying concentrations of KCl (Chem-supply ...
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bioRxiv - Biochemistry 2021Quote: ... Dried lipids were resuspended in 150 ml of CHCl3 and spotted on silica gel high-performance–TLC (HPTLC) plates (Merck, Germany) and resolved with a mixture of chloroform ...
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bioRxiv - Cell Biology 2021Quote: Mitotic HeLa cells were obtained by mitotic shake off of cells arrested using either 200 ng/mL nocodazole (Merck Life Science), 100 nM BI2563 (Adooq Bioscience ...
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bioRxiv - Biochemistry 2021Quote: The initial buffer in the huPrP solution was exchanged to ultrapure water by triplicate diafiltration using an Amicon Ultra 0.5 ml centrifugal filter (Merck & Co., USA) with an NMWL cutoff of 3 kDa ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with Milli-Q water (18.2 MΩ cm) to 50 mL and syringe filtered to <0.2 μm (Merck-Millipore, Burlington, USA) prior to analysis by inductively coupled plasma mass spectrometry (icapQ model ...
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bioRxiv - Plant Biology 2021Quote: ... Each exudate sample was purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Plant Biology 2020Quote: ... A spore suspension (1 × 1011 spores/ml) produced from the maintained inoculum was filtered with a nylon filter with 20µm pore size (Merck Millipore Ltd.) to capture P ...
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bioRxiv - Systems Biology 2021Quote: ... Excess FSBA reagent was removed by ultracentrifugation with 15 mL 10 K MWCO Amicon® Ultra-4 centrifugal filter units (Merck) at 3,500 × g at RT ...
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bioRxiv - Microbiology 2021Quote: ... The diluted solution was concentrated to approximately 5 mL using 30 kDa molecular weight cutoff Amicon Ultra centrifugal filters (Merck KGaA), passed through a Millex-GP syringe filter with 0.22 μm pore size (Merck KGaA) ...
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bioRxiv - Immunology 2022Quote: ... Th1 cell differentiation was induced by culturing CD4 T cells with antigen presenting cells treated with 50μg/ml mitomycin C (Merck, Darmstadt, Germany) in the presence of 1μg/ml OVA323-339 (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Microbiology 2022Quote: Bacteria were enumerated from the lung homogenate and blood by dilution in PBS and plating onto solidified LB agar containing 50ug/ml rifampicin (Merck, UK).
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bioRxiv - Immunology 2022Quote: ... Inhibition of respiratory burst was performed by adding 50 µg/mL of catalase to digest H2O2 and 10 µM of Diphenyleneiodonium chloride (DPI) to inhibit NADPH oxidase (Sigma-Aldrich, Merck) during the overnight coculture ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Microbiology 2021Quote: ... After incubation the mixtures were buffer exchanged in centrifugal filter units (Amicon Ultra, 0.5 ml, 10 K, Merck Millipore, Darmstadt, Germany) washed three times with 50 µl of sodium phosphate buffer (20 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... the HA-GlmS line was transfected with the pHGBrHrBl-1/2 construct in order to episomally express cytosolic GFP then selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). To generate Pfcerli2SLI-TGD ...
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bioRxiv - Molecular Biology 2020Quote: ... all cell lines were seeded in black-walled 96 well plates at a density of 1×104 cells/well or and were maintained in the dark after induction of optogenetic receptor expression through 24 hours incubation in serum-free medium containing 100ng/ml doxycycline hyclate (Merck, #D9891). Optical stimulation was performed at room temperature using either a custom-built Arduino-controlled LED array device using 470nm LEDs (Ballister et al. ...
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bioRxiv - Microbiology 2022Quote: ... and the pellet was pre-treated for disruption of the bacterial cell wall with 300 μl of lysozyme solution in Tris-EDTA buffer (TE) (10 mg/ml, Sigma-Aldrich/Merck), incubated at 37°C for 60 min ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation for 5 minutes at 18 000 g the DNA pellet was washed twice with one mL 70% Ethanol (Merck Millipore) followed by 5 minutes centrifugation at 18 000g ...
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bioRxiv - Plant Biology 2021Quote: ... were moved to new tubes and concentrated at 14,000×g for 10 min at 4 °C using Amicon ultra-0.5 ml centrifugal filters (catalog number Z677094, Merck millipore, Sweden). Thylakoid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The chemoreflex was activated by systemic bolus intravenous injections of 0.1 mL of KCN (40 μg per rat; Merck, Darmstadt, Germany) following procedures described by Franchini & Krieger (1993 ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing the desired His6-tagged protein were concentrated to 2 ml using Amicon® Ultra Centrifugal Filters (10,000 MWCO, Merck Millipore), and were directly injected into a size-exclusion chromatography column (Superdex 75 16/60 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 2 μl of the enzyme β-glucuronidase (from Helix from Pomatia enzyme aqueous solution, ≥ 100.000 units/mL; Merck, Darmstadt, Germany) to deconjugate DEHP metabolites and BPA ...
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bioRxiv - Microbiology 2020Quote: ... the apical medium was removed and basolateral medium was switched to differentiation medium ± 10 ng/ml IL-13 (IL012; Merck Millipore). Differentiation medium consisted of a 50:50 mixture of DMEM-H and LHC Basal (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions corresponding to the elution peak were selected and concentrated to 2-50 mg/mL through the use of Amicon® Ultra-15 Centrifugal Unit (Merck), frozen and stored at −80°C for downstream experiments ...
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bioRxiv - Immunology 2022Quote: ... The remaining volume was spun down at 500x g for 5 minutes and resuspended in 12 ml FACS buffer (PBS [Gibco]+ 0.5% BSA [Merck] + 2mM EDTA). The suspension was transferred over a 70 μm SmartStrainer (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2022Quote: ... was used to mimic tissue-like scattering conditions and Nigrosin (0.62 v/v% of Nigrosin stock solution [0.5 mg/mL Nigrosin in deionised water, Merck, 8005-03-6]) was added to mimic tissue-like absorption.
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: EpiSCs were derived from E14Tg2A Oct4-IRES-Puro ESCs by culturing them on dishes coated with 10ng/ml fibronectin (Merck Millipore), in N2B27 supplemented with 20ng/ml Activin A and 12ng/ml Fibroblast growth factor 2 (FGF2 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... 100 mL of exudate were purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... they were mechanically disrupted by a 21G needle inside a 3 ml DLD dissociation solution (PluriSTEM® Dispase-II Solution, Liberase, #5401119001 DNase I, #10104159001, all from Merck), and incubated for 20-30 min at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... and 160 U/mL SUPERase•In™ RNase Inhibitor) and incubated with anti-FLAG® antibody magnetic beads (Merck, Cat# M8823) overnight at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Viability analysis was performed by staining parasites in culture medium with 0.01 mg/ml of propidium iodide (P4864, Merck Life Sciences). For cell cycle and PAD1 expression analysis ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).