Labshake search
Citations for Merck :
5501 - 5550 of 5744 citations for Trans Dcca 100 Ug Ml In Acetonitrile D3 1 Carboxyl 13C2 99%;1 D 97% 97% Chemical Purity since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... 100 mM EDTA or 200 mM Imidazole) and concentrated up to 0.5 ml using Amicon Ultra-15 centrifugal filters with MWCO of 50 kDa (Merck, Germany). The sample was then applied to a size exclusion chromatography on a Superdex 200 increase 10 /300GL column equilibrated with wash buffer ...
-
bioRxiv - Bioengineering 2021Quote: ... hiPSCs were transduced with LV particles at MOI of 5 in the presence of 5 µg/mL of polybrene (Merck). Stably transduced cells were obtained upon selection with 0.3 µg/mL of puromycin for 6 days ...
-
bioRxiv - Neuroscience 2020Quote: ... The implantation surgery was conducted under pentobarbital anesthesia (60 mg/kg, i.p.; dissolved in 0.9% sodium, 10 mg/ml, Merck, Darmstadt, Germany). The Hip and PFC recording electrodes consisted of two twisted pairs of perfluoroalkoxy (PFA)-coated stainless steel wires (diameter 0.002’’ ...
-
bioRxiv - Biophysics 2021Quote: ... The eluted sample was exchanged into 20 mM Tris buffer and concentrated to 5 ml by Amicon Ultra-15 3 kDa (Merck). The sample was purified by 320 ml of HiLoad Superdex with a flow rate of 1 ml/min using the FPLC systems.
-
bioRxiv - Cell Biology 2021Quote: Apoptotic vesicles were isolated from MSCs undergoing apoptosis for 24h using 500ng/ml of an Anti-Fas Ab (3510771, Merck) diluted in RPMI medium ...
-
bioRxiv - Biochemistry 2021Quote: ... the pure huPrP protein (i.e., with the HisTag removed) was concentrated using an Amicon Ultra 0.5 ml centrifugal filter (Merck & Co., USA) with an NMWL cutoff of 3 kDa ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were further separated by size exclusion chromatography (HiLoad 26/600 Superdex 75, Cytiva) and concentrated using Ultra-4 or 15 mL centrifugal filter devices (Amicon, Merck). Correct size and high purity were verified via SDS-PAGE and LC-MS analysis ...
-
bioRxiv - Microbiology 2020Quote: ... cultures were plated on LB (1.6%) agar with 25 μg/mL kanamycin (AppliChem Lot# 1P0000874) or chloramphenicol (Merck Lot# 2430B61), respectively.
-
bioRxiv - Microbiology 2021Quote: 2mL SARS-CoV-2 P4 supernatant containing 2 x 107 pfu/mL was purified using an Amicon Ultra column (MWCO 100kDa; Merck) by centrifugation and washing in PBS at 2,000 x g ...
-
bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
-
bioRxiv - Systems Biology 2021Quote: ... The following starting material was loaded onto an Amicon® Ultra-0.5 mL centrifugal filter unit (nominal molecular weight cut-off of 30,000; Merck Millipore): 1 ...
-
bioRxiv - Microbiology 2020Quote: ... The crude fractions were dissolved in EA (10 mg/ml) and 10 µl from each were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a binary mobile phase consisting of Chloroform ...
-
bioRxiv - Microbiology 2020Quote: ... Twenty µl of the EA fraction (10 mg/ml) were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a quaternary mobile phase consisting of Benzene ...
-
bioRxiv - Microbiology 2020Quote: ... The eluate was neutralised immediately using 1.5 M Tris pH 8.6 and then buffer exchanged to PBS using a 15 ml 30 kDa MWCO centrifugal filter (Merck Millipore).
-
bioRxiv - Cancer Biology 2020Quote: ... Virus containing media was collected and PUER cells were transduced by spin-infection in the presence of 12 μg/ml polybrene (Merck) at 850 g for 2h ...
-
bioRxiv - Cancer Biology 2020Quote: ... MCF7 cells were transduced with lentiviral particles at 75% confluency in the presence of 10 μg/ml polybrene (Merck, Germany). 24h after transduction virus containing medium was replaced with selection medium for the respective expression constructs to start the selection ...
-
bioRxiv - Cell Biology 2021Quote: ... THP-1 cells were differentiated to macrophages by complementing the media with 10 ng/ml phorbol 12-myristate 13-acetate (#P8139, Merck) for seven days ...
-
miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... 20 μL of the MTT solution was added to each well (5 mg/ml, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Then ...
-
bioRxiv - Cell Biology 2020Quote: ... T24 cells for confinement experiments were exited either in a 0.1% gelatine coated T12.5 flasks or in a 20 μg/ml laminin (Merck Millipore, #CC095) coated T12.5 flask incubated for 3h at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Cell medium was replaced with 3 mL medium containing 20 μL of the purified lentivirus and 3μl polybrene transfection reagent (Merck Millipore). Medium was supplemented with 10 µg/mL puromycin for selection of successfully transduced cells two to three days after transduction.
-
bioRxiv - Cancer Biology 2021Quote: Urine samples were first adjusted to pH 8.0 using 500 mM ABC supplemented with EDTA-free protease inhibitor and then filtered through an Amicon Ultra-0.5 mL Centrifugal Filter Device (10 kDa, Merck Millipore). Collection of the retentate was performed by flipping the filter device upside-down and conducting centrifugation at 1,000g for 2 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated to 2 mg/mL using centrifugal filters with either 3 kDa or 30 kDa cut-off (Amicon Ultra-0.5, Merck/Millipore) and the samples were centrifuged at 100,000 g ...
-
bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
-
bioRxiv - Microbiology 2021Quote: ... protoplasts were overlaid with ½-strength PDA cooled-down to ~50°C and supplemented with 50 mg/ml hygromycin B (Merck). Transformants appearing on the PDA surface ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 72h with the indicated concentrations of the respective drugs and further incubated for 3h with 5 mg/ml MTT (Merck). Finally ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... while the rat abdominal aortic rings (15 mg wet mass/mL) were pre-incubated for 10 min in Medium 199 (Merck). The examined enzyme reaction started after pipetting the tracer substrate 1-14C-AA (3.7 kBq ...
-
bioRxiv - Biochemistry 2022Quote: ... for 30 min at room temperature – was diluted to ∼0.002 mM DTT by three rounds of dilution/concentration in an Amicon Ultra 15 mL 3K Centrifugal Filter (Merck Millipore). TEV protease cleavage was performed overnight at room temperature.
-
bioRxiv - Microbiology 2022Quote: ... 1.5×104 conidia of each strain were inoculated in 300 µL of RPMI in the presence of 8 µg/mL voriconazole (European Pharmacopoeia, -EP- Reference Standard, Merck) and incubated for 48 hours at 37 °C with occasional shaking ...
-
bioRxiv - Bioengineering 2019Quote: ... Removal of excess prongs was done first with washing in 50 kDa MWCO 0.5 mL Amicon centrifugal filters (Merck Millipore) and then by means of Streptavidin-coated magnetic beads (DYNAL MyOne Dynabeads Streptavidin C1-Thermofisher Scientific ...
-
bioRxiv - Developmental Biology 2020Quote: ... pregnant mice were injected intraperitoneally with 375 μg doxycycline (75 μl of a filter sterilized, 5 mg/ml solution of Doxycycline Hyclate (Merck) dissolved in PBS) ...
-
bioRxiv - Microbiology 2019Quote: ... 100 μL of the faeces mixture was added to 10 mL of Selenite Broth (19 g/L selenite broth base, Merck, UK ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... organoids generated from the H9 doxycycline-inducible ZEB2 (HumiZEB2) cell line were treated with 1.7 μg/ml doxycycline (Merck, PHR1145) during the first 5 days of the protocol.
-
bioRxiv - Cancer Biology 2020Quote: ... Gaithersburg, MD, USA) and 8-μm-pore polycarbonate membranes (Nucleopore, Pleasanton, CA, USA) coated with 10 μg/ml fibronectin (Merck) as described elsewhere (Prevete et al. ...
-
bioRxiv - Cell Biology 2020Quote: The dry polymer powders were dissolved in cell culture water (B Braun Melsungen AG, Germany) to a final concentration of 2.4 mg/ml containing 0.5% NaCl (Merck KGA, Germany). This stock solution was sterile filtered through a 0.22mm filter (Sarstedt ...
-
bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
-
bioRxiv - Neuroscience 2021Quote: ... A sample of the supernatant plasma (0.3 mL) was loaded onto the reservoir of a Centrifree® Ultrafiltration device (Merck Millipore Ltd ...
-
bioRxiv - Immunology 2020Quote: ... or a shRNA specific for BBS1 (J KD) and selecting in puromycin-containing medium at the final concentration of 3.5 μg/ml (Merck, #P8833). Knocked-down Jurkat cells were routinely checked for BBS1 expression by immunoblotting ...
-
bioRxiv - Molecular Biology 2020Quote: ... Eluted fractions containing HCA II were pooled and concentrated to ~20 mg/ml by using Amicon Ultra Centrifugal Filter Units (Merck) with a molecular weight cut-off 10 kDa.
-
bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
-
bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2 buffer at a final concentration of 5 mg/mL using three consecutive cycles of freezing in liquid nitrogen and thawing in an ultrasonic bath (Merck). The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.) ...
-
bioRxiv - Cancer Biology 2022Quote: ... in HEPES buffer was exchanged with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0) using YM30K centrifugal filter unit (Amicon ultra-0.5 mL, Regenerated cellulose, Merck Millipore Ltd.,). It was further diluted with 0.1 M Na2CO3/NaHCO3 buffer (pH = 9.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U/mL benzonase (Merck). After 1h incubation at 37°C ...
-
bioRxiv - Biophysics 2022Quote: ... CM was concentrated ≈300 times to 0.5 mL using a Centricon Plus-70 centrifugal filter device with a 10K nominal molecular weight limit (Merck Millipore).
-
bioRxiv - Biophysics 2022Quote: ... Fraction 4-6 of three different columns were pooled and concentrated to 0.5 mL using an Amicon Ultra-15 10K filter (Merck Millipore). This was then placed on top of the gradient.
-
bioRxiv - Developmental Biology 2022Quote: ... The cumulus mass containing the MII oocytes and zygotes was transferred to a drop of M2 medium containing 300 µg/ml hyaluronidase (H4272, Merck) to release the cumulus cells ...
-
bioRxiv - Microbiology 2022Quote: ... ljungdahlii strain and its derivatives was carried out in 125 mL serum bottles (Sigma-Aldrich, Merck Life Science, Gillingham, UK) with a working volume of 30 mL ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were centrifuged at 3041 × g for 30 minutes at 4°C and supernatant was applied to a 500 mL Stericup-HV sterile vacuum filtration system (Merck) with a pore size of 0.22 µm ...
-
bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...