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Citations for R&D Systems :
4601 - 4650 of 4704 citations for Recombinant Human IIntercellular Adhesion Molecule 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then sections were incubated overnight at 4 ◦C with goat polyclonal anti-S100a10 antibody (1:200; AF2377, R&D Systems, Minneapolis, MN, USA), rabbit polyclonal anti-5HT1B Receptor antibody (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... Total VEGFR2 expression (0.1% Tx-100 permeabilized cells) was compared to surface level VEGFR2 (non-permeabilized cells) using a biotinylated VEGFR2 antibody (R&D Systems; BT10543). Images were collected using a W1 spinning disk confocal microscope.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Serum levels of cytokines (IL-6, TNF-α, MCP-1 and IL-10) were measured by corresponding ELISA kits (R&D Systems, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... They were then incubated overnight at 4°C with a goat anti-SAA primary antibody (1:500; AF2948, R&D Systems, Minneapolis, MN). This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Genomics 2024Quote: ... Progenitors were then resuspended in a 10% dilution in volume of FBS and 100ng/mL CSF-1 (R&D Systems; 216-MC-500) in Gibco™ RPMI-1640 media (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... A multiplex primary antibody mix was prepared in milk-free antibody diluent using Rabbit anti-DYKDDDK antibody (R&D Systems MAB8529; 1:1200) and mouse anti-Beta actin (R&D Systems MAB8929 ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived-dendritic cells (moDC) were obtained by incubation of monocytes with 1 ng/mL of granulocytes-macrophages colony-stimulating factor (GM-CSF, R&D Systems, France) and Interleukin-4 (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were blocked in 3% bovine serum albumin for 30 min and incubated with the following primary antibodies overnight at 4°C: β-III tubulin (1:1000, MAB1195, R&D system), rabbit anti-MAP2 (1:1000 ...
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bioRxiv - Pathology 2022Quote: ... for 1 hour at room temperature followed by overnight incubation with primary antibodies for IL-31RA (15 µg/ml, R&D Systems AF2769) and CHRM3(1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were fixed in 4% paraformaldehyde solution as previously described.39 Membranes were immunoblotted for AXL (AF154 R&D Systems at 1:500), MerTK (ab52968 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies targeting the following proteins were used at the indicated dilutions and obtained from the denoted companies: Robo3 1:300 (R&D systems, #AF3155), GluN1 NMDAR 1:1000 (SySy #114011) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurospheres were transferred to Matrigel-coated plates and cultured in Neural Medium (DMEM/F12 medium supplemented with N2, N2, and 20 ng ml-1 of basic FGF (R&D Systems Inc.)) for two weeks to generate a monolayer of hNPCs ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
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bioRxiv - Developmental Biology 2023Quote: pH 6.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: pH 9.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), SOX9 (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... alone or sequentially with 100 μL/mL polylysine and 400 ng/mL netrin-1 (R&D systems, catalog number 6419-N1-025/CF), and cultured in Neurobasal Medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with assay medium supplemented with 10 ng/ml transforming growth factor-beta 1 (hereafter referred to as TGF-β, 240-B-002, R&D Systems) for 24 h or 72 h (Peinado et al. ...
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bioRxiv - Cell Biology 2024Quote: ... slides for ACE-2 single stain and slides for ACE-2 stain were incubated with the Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: TNF-α and IL-6 in BAL fluids and serum samples were measured using ELISA kit (TNF-α: MTA00B; IL-6: M6000B-1) from R&D Systems, according to the recommended protocols from the manufacturer.
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bioRxiv - Immunology 2024Quote: ... NJ;). CCL18(1-69) (cat. no. 394-PA) and mCCL1 (cat. no. 845-TC) were purchased from R&D Systems (Minneapolis, MN, USA;). AF687-labelled CCL18 (AF-CCL18 ...
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bioRxiv - Neuroscience 2021Quote: ... These rats were then administered 50 μl of NRG-1β (1% BSA in PBS) or 50 μl of vehicle (1% BSA in PBS) treatment (20 ug/kg; EGF-like domain, R&D Systems, Minneapolis, MN) via bolus injection into the ICA through the ECA immediately before MCAO ...
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bioRxiv - Neuroscience 2021Quote: ... every third sagittal section of tissue containing the left fastigial nucleus was blocked with 5% bovine serum albumin for one hour at room temperature prior to incubation in primary antibody goat anti-osteopontin overnight at 4°C (1:300, R&D Systems AF808; RRID:AB_2194992). Tissue was then rinsed and incubated in donkey anti-goat Alexa Fluor 594 for 2 hours at room temperature (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: IGF-1 levels in the serum and tissues (liver, gastrocnemius, and brain cortex) were determined using Quantikine mouse IGF-1 immunoassay from R&D systems (MN, USA). Serum samples were diluted in calibrator diluent provided in the kit at 500-fold dilution and assayed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells (1 × 104) were seeded on 8 µm Boyden chamber inserts (Becton Dickinson Labware, Bedford, MA)(23) coated with fibronectin (R&D Systems, Minneapolis, MN) (5 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained in 0.5% fatty acid-free albumin in DPBS using 1:20 mouse IgG3 SSEA4-488 clone MC-813-70 (R&D Systems, FAB1435F, lot YKM0409121) and 1:20 mouse IgM TRA-1-60-488 clone TRA-1-60 (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... secreted IL-2 samples were diluted 1:10 and subsequently analyzed using Mouse IL-2 Quantikine ELISA Kit (R&D Systems Inc, Minneapolis, MN), as per manufacturer’s specifications.
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bioRxiv - Neuroscience 2022Quote: ... Non-specific blocking for 30 minutes in 8% horse serum in KPBS was completed before incubation in primary antibody (anti-HPLN1 1:150, R&D Systems 2608-HP) for 48 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 150 mM NaCl) with 0.1% Tween 20) for 30 minutes and probed with primary antibodies: polyclonal goat anti-C3d (R&D systems, AF2655, 1:1000), polyclonal sheep anti-Sez6L2 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... fourth weeks of infection were collected to evaluate the concentration of MCP-1 and TNF-α by using ELISA kits according to the manufacturer’s protocols (R&D Systems, Rennes, France), the mean minimum detectable dose of human MCP-1 was 1.7 pg/mL and 4.00 pg/mL for human TNF-α ...
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bioRxiv - Genomics 2020Quote: Plasma was used to assess circulating monocyte chemoattractant protein (MCP)-1 and C-reactive protein (CRP) via ELISA following manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA) (1).
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bioRxiv - Cell Biology 2021Quote: ... media was replaced with RPMI supplemented with B27 without insulin and containing 1 µM CHIR99021 and 5 ng/ml BMP4 (R&D systems #314-BP). After an additional 48 hours (Day 3) ...
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bioRxiv - Cancer Biology 2021Quote: ... Then membranes were blocked in 5% non-fat milk in TBS-T for 30 minutes at room temperature and incubated with primary antibodies against Robo1 (R&D Systems, 1:500), Robo2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed and stained as described in (Rijal et al. 2019) using a 1:500 dilution of anti-CD66 (#MAB2244, R&D Systems, Minneapolis, MN), anti-CD62-L (#555542 ...
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bioRxiv - Cell Biology 2022Quote: ... and stained for 30 minutes on ice with a 1:50 dilution of AlexaFluor-647 conjugated anti-LTB4R antibody (R&D Systems; catalog #: FAB099R) in FACS buffer (0.5% bovine serum albumin and 0.05% sodium azide in PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... Filters were blocked in 5% nonfat milk in TBS-Tween 20 (0.1%) and incubated overnight with rabbit anti-NrCAM (R&D Systems AF8538, 1:1000), mouse anti-Rac1 (BD Biosciences # 610651 ...
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bioRxiv - Pathology 2020Quote: ... SR-AI and CD-36 proteins were pulled down from MΦ membrane extracts as described above for TLR-4 using anti-SR-A (R&D System, 1:1000) and anti-CD36 (R&D System ...
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bioRxiv - Bioengineering 2020Quote: ... The slides were then incubated overnight in the dark at 4°C with primary antibodies against: Nephrin (NPHS1,AF4269, R&D Systems, 1:300), Lotus Tetragonolobud Lectin (LTL ...
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bioRxiv - Bioengineering 2019Quote: hESCs were differentiated to SC-β cells as previously described9 by culturing in the following conditions in order: Stage 1: 3 d with 100 ng/ml Activin A (R&D Systems; 338-AC) and 3 μM CHIR99021 (Stemgent ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were transferred to a membrane using the iBlot2 system and incubated overnight at 4°C with primary antibody (anti-Gli3, 1:500, AF3690, R&D Systems, Minneapolis, MN). Membranes were then incubated in secondary antibody (anti-goat Alexafluor 680 ...
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bioRxiv - Cell Biology 2021Quote: ... directly after UV irradiation or pre-treated 1 hour before irradiation with proteasome inhibitor MG132 (Enzo, 50 µM) or NEDD8 E1 Activating Enzyme Inhibitor (NAEi) MLN4924 (R&D systems, 10 µM) where indicated ...
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bioRxiv - Cell Biology 2020Quote: ... Retinal crossections (12 µm) were processed for immunostaining using the following antibodies: rat monoclonal to CD45 antibody (clone 30-F11, R&D Systems, 1:50). Sections were pre-incubated with 5% goat serum (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with anti-CD31 primary antibody (1:200, #550274, BD Biosciences, San Jose, CA, USA; #BBA7, R&D systems, Minneapolis, MN, USA) and Alexa Fluor 594-conjugated secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
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Stromal STAT5-mediated trophic activity regulates hematopoietic multipotent progenitor niche factorsbioRxiv - Cell Biology 2022Quote: ... fresh DMEM medium with reduced FBS (2%) was changed and the supernatant was harvested 24 hr later for IGF-1 and SDF-1α ELISA assays (R&D Systems, Minneapolis, MN).
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bioRxiv - Neuroscience 2022Quote: ... The cannula was connected to an osmotic minipump filled either with vehicle solution (PBS containing 0.1% BSA as a carrier) or 35 μg/ml of soluble TNF receptor-1 (sTNFR1, R&D Systems Inc. Minneapolis, MN).
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bioRxiv - Microbiology 2023Quote: ... Huiskonen (University of Oxford) and incubated for three days at 37 °C in the presence of 1 μg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half a volume of binding buffer (25 mM HEPES ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the samples were then longitudinally oriented and cut to 15µm thickness before being stained with primary antibodies to mouse CD31 conjugated to Alexa Fluor 488 (R&D Systems, #FAB3628G, 1:100), CD31 (BD Pharmingen ...
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bioRxiv - Neuroscience 2023Quote: ... The cell lysates (20 μg) were electrophoresis using SDS gels and transferred to nitrocellulose membranes and the incubated with TrkB antibody (1:1000, R&D systems, goat, AF1494), CRHR1 antibody (1:1000 ...