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Citations for R&D Systems :
4401 - 4450 of 4704 citations for Recombinant Human IIntercellular Adhesion Molecule 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Afterwards cells were incubated with primary antibody against PDGFRa (goat, 1:200, AF-307-NA, R&D Systems, MN, USA), CD90 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... For proximity ligation assays VE-cadherin was detected using mouse VE-cadherin antibody (1:200, R&D systems, Goat, AF1002). c-Src was detected using anti-Src (GD11 clone ...
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bioRxiv - Immunology 2022Quote: ... TNF and IL-1 receptor antagonist (IL-1Ra) were measured in thawed supernatants using commercial ELISA kits (R&D Systems). IL-1Ra was measured because it inhibits the binding and bioactivity of IL-1β85 ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml CD40-ligand-HA with 5 ng/ml anti-HA antibodies (both R&D Systems, Minneapolis, USA), and Type B CpG OND D-SL01 (InvivoGen ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems, UK). Plates were washed and 100 ul of substrate solution ...
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bioRxiv - Cancer Biology 2020Quote: ... 8 μm cryosections were fixed in acetone and incubated with goat-anti-mouse CD31 (R&D system, #AF3628, 1:200) and rabbit-anti-FITC (Biorad ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibody information is as following: Goat anti-mouse aminopeptidase N/ANPEP (CD13; R&D systems; AF2335; 1:100), Rat anti-mouse vascular adhesion molecule (VCAM1 ...
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bioRxiv - Microbiology 2021Quote: ... 10ng/ml interleukin 4 (IL-4) and 10ng/ml transforming growth factor beta 1 (TGFβ1) (R&D systems, Minneapolis, MN), and used between days 7-9 of differentiation.
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bioRxiv - Cancer Biology 2021Quote: ... or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200, AF6249, R&D Systems, Minneapolis, MN, USA) in Antibody Diluent Buffer from Ventana Medical Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies and dilutions used for immunohistochemistry in this study were the following: PDGFRA (goat, 1:70; R&D Systems); GPNMB (goat ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4 and then incubated overnight at 4°C with mouse monoclonal anti-CD154 (1:50, R&D systems, MAB617) and rabbit anti-calbindinD28k (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro ubiquitylation assay reactions in 1 x ubiquitin conjugation reaction buffer (R&D systems Cat. # B-70) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Molecular Biology 2019Quote: 10 μl in vitro SUMOylation assay reaction in 1 x SUMO conjugation reaction buffer (R&D systems Cat. # B-60) contains 10 mM Mg2+-ATP solution pH 7.0 (R&D systems Cat ...
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bioRxiv - Physiology 2020Quote: ... followed by overnight incubation at 4 °C with primary antibodies against UCP1 (1:1000, R&D systems, Minneapolis, MN, USA), 14-3-3ζ (1:1000 Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were applied overnight at 4 °C: goat anti-OTX2 (1:500, R&D Systems, cat# AF1979), mouse anti-CDX2 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were visualized by incubation with HRP-conjugated IgG Secondary antibody (anti-goat, 1:5000; # HAF017, R&D Systems, goat anti-rabbit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then resuspended in 20 μl of 1× ubiquitin reaction buffer containing 200 nM UBE1 E1 (R&D systems, U-110), 1 μM UbcH5a E2 (Enzo ...
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bioRxiv - Developmental Biology 2021Quote: ... At day 1 the medium was changed to SFD media supplemented with Activin A (100 ng/ml, R&D Systems), basic FGF (bFGF ...
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bioRxiv - Developmental Biology 2022Quote: ... ITGA8 was stained by the use of polyclonal goat anti-Integrin alpha 8 antibody (1:50; R&D Systems, Minneapolis) and GDNF was stained by the use of polyclonal rabbit anti-GDNF antibody (1:100 ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The following secondary antibodies were used at a 1:5000 dilution: goat anti-rabbit IgG-HRP (R&D Systems; HAF008) and goat anti-mouse IgG-HRP (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used for our IHC study: Goat anti-Serpina3n (1:3000, R&D System, Cat# AF4709, RRID:AB_2270116); Mouse anti-CC-1 (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells were first differentiated into macrophages with 50 nM Phorbol-12-Myristate-13-Acetate (PMA) (R&D systems) for 72 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... carboxylesterase 1 (CES1) and trans-epoxy-succinyl-L-leucylamino(4-guanidino)butane (E-64) were purchased from R&D Systems. The respective genes coding for the human recombinant enzymes Histidine triad nucleotide binding protein 1 (HINT1) ...
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bioRxiv - Immunology 2023Quote: ... mice were given SL-TBI (550cGy) and subsequently received intraperitoneal injections of 1 mg/kg UTPγS (R&D systems, 3279), or 1x PBS as control ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with ice-cold acetone and stained with 1:200 dilution of monoclonal-anti-RBD (R&D Systems, MN, USA), polyclonal-anti-S1- ...
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bioRxiv - Microbiology 2022Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated with the appropriate primary antibody overnight at 4°C: anti-IL1 (goat, 1:1000, R&D Systems), anti-phosphorylated tau (AT8 ...
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bioRxiv - Microbiology 2023Quote: ... The following secondary antibody dilutions were made 1:2000 in PBST: goat α-mouse IgG-HRP (R&D Systems; HAF007) and goat α-rabbit IgG-HRP (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... diluted at 1:1000 in TBST 0.1 % supplemented with 2.5 % BSA or with Goat anti- GAPDH antibody (AF5718, R&D systems) diluted at in TBST-0.1 % supplemented with 2.5 % milk or anti-V5 HRP (Thermofisher scientific ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for immunofluorescence staining: goat anti-mouse VE-cadherin (R&D Systems, AF1002; 1:200), hamster anti-mouse PDPN (Developmental Studies Hybridoma Bank ...
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were incubated overnight at 4°C with the primary antibodies for A2B5 (1:50; R&D Systems, MAB1416) and NG2 (1:300 ...
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... antigen-retrieved in pH6 sodium citrate buffer and stain with primary antibodies including: anti-LYVE-1 (AF2089; R&D System), anti-CXCL8 (MAB208 ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used for immunoblotting: Goat IgG against VE-cadherin (1:500, AF1002, R&D Systems, Minneapolis, MN), pY658 VEC (1:250 ...
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bioRxiv - Microbiology 2023Quote: ... and a mouse anti-SARS-CoV-2 N monoclonal antibody (clone 1035111, R&D Systems, Cat# MAB10474-SP, 1:400) was used as a primary antibody ...
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bioRxiv - Immunology 2023Quote: ... Samples were diluted 1:10 and IL8 was measured using the DuoSet IL8 ELISA kit (R&D Systems cat# DY208) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary OC-CAFs were incorporated into a 3D matrix made of rat tail type 1 collagen (R&D Systems) as previously described 95 ...
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bioRxiv - Bioengineering 2024Quote: ... or Epiregulin (EREG) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#1195-EP-025/CF) with Noggin-Fc (100ng/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and the mixture of Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Secondary Antibody (1:100, NL004, R&D system) and Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: mOSE cells (1×10^6 cells) were plated 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). RNA was collected 7 days post-TGFB1 treatment (RNAeasy Kit ...
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bioRxiv - Cell Biology 2021Quote: ... NHEKs were induced to differentiate by addition of 1.2 mM CaCl2 to growth medium with or without addition of 1 μg/mL ephrin A1-FC peptide (R&D Systems) for the indicated times ...
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bioRxiv - Developmental Biology 2021Quote: The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blotted using the primary antibodies: Ebi3 (2.5 μg/ml; eBioscience) or IL-12/IL-35 p35 (1 μg/ml; R&D Systems), phospho-mTOR (Ser2448 ...
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bioRxiv - Systems Biology 2019Quote: ... CCL2 and CCL5 secreted protein levels in cell supernatants from 1×106 murine BMDM’s was performed by ELISA (R&D Systems) according to manufactureŕs instructions.
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bioRxiv - Immunology 2019Quote: ... then incubated overnight at 4°C in primary antibodies in 0.1% Triton X-100/PBS (rat anti-GP2, MBL, D278-3 at 1:500; sheep anti-SpiB, R&D Systems, AF7204 at 1:50 ...
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bioRxiv - Neuroscience 2019Quote: Antibodies and dilutions used for immunofluorescence staining were as follows: polyclonal goat anti-mouse Pdgfra (R&D Systems, AF1062, 1:500), monoclonal rabbit anti-mouse Olig2 (Abcam EPR2673 ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were differentiated into macrophages and dendritic cells using 50 ng/ml GM-CSF (R&D Systems, https://www.rndsystems.com) + 50 ng/ml M-CSF (R&D Systems ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 1 min at 4°C) pre-incubated with 5 μl TRKB antibody (Goat anti-mouse TRKB Antibody, #AF1494, R&D Systems) and cleared through new Protein-G sepharose tubes ...
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bioRxiv - Microbiology 2021Quote: ... serum was incubated for 2 h with polyclonal sheep IgG anti-LL-37 (2.5 – 20 μg ml−1; R&D Systems) or anti-hNP-1 (2.5 – 20 μg ml−1 ...