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Citations for R&D Systems :
4451 - 4500 of 4704 citations for Recombinant Human IIntercellular Adhesion Molecule 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde followed by permeabilization and staining with primary antibodies for LOXL2 (1:500, R&D Systems) and Tetramethylrhodamine (TRITC)-conjugated Phalloidin (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Gels were then subjected to immunoblot analysis for CHIKV E using an anti-E antibody (1:1000, R&D Systems, MAB97792SP).
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bioRxiv - Developmental Biology 2022Quote: ... Agents were added to the medium at final concentrations of 1 mg/ml for BMP2 (355-BEC-010, R&D Systems), 500 μM for GdCl3 (16506-71 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged and resuspended in mTeSR1 supplemented with 10 μM SB431542 (SB; Stemgent) and 1 μM Dorsomorphin (R&D Systems). 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm ...
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bioRxiv - Cancer Biology 2021Quote: Cells were released from plates by incubating with 0.05% trypsin and then stained with primary antibody (1 μg/106 live cells) against NPY1R (MAB6400; R&D Systems) followed by secondary antibody against human IgG for 30 minutes on ice and/or with PE-conjugated ABCC5 (sc-376965 PE ...
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bioRxiv - Neuroscience 2022Quote: ... Group 1 ligands were derived from mouse transcriptomic data and included: APP (R&D Systems, Cat. 3466-PI-010, 20ng/mL), APOE3 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated in primary antibody (chicken anti-GFP, Aves labs GFP-1010, 1/500; goat anti-PDGFRA, R&D Systems AF1062 ...
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bioRxiv - Immunology 2022Quote: ... then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies: CD4 (1:300; clone GK1.5; R&D Systems); CD44 (1:300 ...
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bioRxiv - Microbiology 2019Quote: ... using known concentrations of commercial rBoIFN-γ (20, 15, 10, 5, 3 and 1 ng; 2300-BG-025, R&D Systems).
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bioRxiv - Neuroscience 2020Quote: ... OPCs were treated with LCN2 (1, 2.5, 5, 10, 20, 40, 80, or 160 ng/ml, R&D Systems, Shanghai, China) or vehicle control (0.0002% BSA ...
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bioRxiv - Immunology 2020Quote: ... Plasma was assayed in duplicate for CCL2 using the same CCL2/JE/MCP-1 DuoSet ELISA kit (R&D Systems™) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were utilized for immunoblots: goat anti-CD31 antibody (1:500, #AF3628, R&D Systems, Minneapolis, MN, USA) for mouse ...
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bioRxiv - Cell Biology 2021Quote: ... 1% (v/v) B27 Serum-Free Supplement (50x) (Life Technology, 17504044) and 50 ng/ml FGF7 (R&D System, 251-KG). For posterior foregut stage (d7-d8) ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against myeloperoxidase (1:150 dilution; R&D Systems, AF3667), citrullinated histone H3 antibody (1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... Sanofi-aventis Cat# NAC2004–5843-01) and interleukin-4 (IL-4; 1000 IU/mL; R&D Systems Cat# 204-1 L). Mature ...
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bioRxiv - Immunology 2020Quote: Recombinant mouse IL-27 (<1.0 EU per 1 μg of the rmIL-27 protein by the LAL method) was purchased from R&D systems.
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated for 1 hr at room temperature (RT) with anti-goat HRP conjugated antibody (R&D Systems #HAF109) at 1:2000 in TBST 1% milk ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from CH12 cells cultured for 3 days with TGF-β (1 ng/ml; R&D Systems Europe), IL-4 (5 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Myeloid progenitor cells were treated in suspension for 1 h with 5μg of IgG2B isotype control (clone 141945; R&D Systems) or αTREM2 (rat clone 237920 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fixed cells were stained with 1:100 dilution of APC-conjugated anti-SOX-17 (R&D Systems, Minneapolis, MN, USA; IC1924A) for 30 mins at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies used for western blot were used as follows: rat anti-IL-33-unconjugated (1:1000; R&D Systems MAB3626), rat anti-MCP1/Mcpt1 (1:1000 ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were then probed with anti-HSD17β1 polyclonal antibody (Abnova, H00003292-M03A, 1:1000 dilution, overnight 4°C; R&D systems, MAB7178 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated overnight at 4°C with one of the following antibodies: FGE (R&D Systems, MAB2779, 1:1000), LAMP1 (DSHB ...
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bioRxiv - Microbiology 2023Quote: ... Relative expression was calculated against a 2% input control.Samples were then normalized to uninfected cells stimulated with universal type 1 interferon (R&D Systems).
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bioRxiv - Neuroscience 2023Quote: ... For hCO the neural medium was supplemented with the growth factors EGF (20 ng ml−1, R&D Systems, 236-EG) and FGF2 (20 ng ml−1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... resuspended in SDS loading buffer and subjected to Western Blot against phosphorylated Tyrosine (1:1000, BAM1676, R&D systems, Mineapolis, USA) or Traf6 antibody (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 and IGF-1 serum levels were assessed using the Mouse IL-6 Quantikine ELISA Kit (R&D Systems, M6000B) and Mouse IGF-1 Quantikine ELISA kit (R&D Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFβ concentration in the cell free BALF was calculated using Mouse TGFβ 1 DuoSet Elisa (R&D Systems Cat DY1679-05) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated for 1 hour at RT with anti-PAR/pADPr monoclonal antibody (R&D Systems cat# 4335-MC-100) diluted 1:250 in 3% BSA-PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... United States) or equine TNF-α (1 ng/mL; catalog no. 1814-ET-025/CF, R&D Systems, Minneapolis, United States) in combination with LPS ...
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bioRxiv - Cell Biology 2023Quote: ... Chicken anti pan-neurofascin (against residues 25-1031 of rat neurofascin, 1:400 for IF) was from R&D System (#AF3235). Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain ...
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bioRxiv - Cancer Biology 2024Quote: ... blocking in 10% donkey serum was followed by overnight incubation at 4°C with anti-CD31 (1:100; R&D Systems) and subsequent incubation with a donkey anti-goat AlexFluor594 secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... and reseeded on laminin-521 coated plates at a split ratio of 1:2.5 in CPN medium supplemented with 20 ng/mL fibroblast growth factor 2 (FGF2; R&D Systems) and 10 µM Y-27632 ...
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bioRxiv - Cancer Biology 2021Quote: ... melanocytes were suspended in 1 ml medium and seeded on 0.5% collagen (Cultrex, R&D Systems, Minneapolis, MN, Cat # 3442-050-01), in 24 well plates for three days.
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bioRxiv - Cell Biology 2020Quote: ... mOSE cells (1×10^6) were first plated as monolayer cultures 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). Four days after the addition of TGFB1 ...
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bioRxiv - Cell Biology 2020Quote: ... Once the cells were adherent the cells undergoing differentiation were cultured in basal medium supplemented with 1% v/v Adipogenic Supplement (R&D Systems) while the control cells not undergoing differentiation were cultured in basal medium ...
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bioRxiv - Cell Biology 2020Quote: ... sections were incubated over night at 4 °C with the following antibodies diluted in PBS 2.5% BSA: goat anti-CD140a 1:80 (R&D Systems AF1062) and rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S) ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... diluted 1:200 and analyzed for adiponectin by ELISA following the manufacturer’s instructions (Mouse Adiponectin/Acrp30 Quantikine ELISA Kit, R&D Systems, MRP300). Absorbance (450 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... micropattern cultures were fed in lung induction medium (LIM) which contains cSFDM supplemented with 50 ng*mL−1 KGF (or indicated in each experiment; R&D systems), 10 ng*mL−1 BMP4 (R&D systems) ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used for immunofluorescence in this study were as follows: anti-mouse 1:150 GPR34 (R&D Systems; Cat. No. MAB4617), anti-rabbit 1:1000 IBA1 (Wako ...
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bioRxiv - Neuroscience 2021Quote: ... The ELISA was performed according to the published protocol [43] using these antibodies: anti-TrkB goat 1:1000 (#AF1494, R&D systems), anti-SorCS2 rabbit 1:2000 (#bs-11963R BIOSS) ...
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bioRxiv - Molecular Biology 2021Quote: Retinal organoids on day 90 of differentiation were incubated at RT for 1 hour in PBS with either a goat anti-ACE2 antibody (AF933, R&D Systems) or a normal goat IgG control (AB-108-C ...
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bioRxiv - Immunology 2019Quote: ... and homogenates were diluted to 2 mg/mL protein in PBS and were subsequently diluted 1:2 in Reagent Diluent (R&D Systems). CXCL10 protein was quantified using the Mouse CXCL10 Duoset ELISA kit (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... 96-well flat bottom plates were pre-coated with 20 μg/ml of an agonist anti-TREM-1 mAb (clone 174031; R&D Systems) overnight at 37°C ...
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bioRxiv - Immunology 2019Quote: ... a total of 1 × 106 RAW264.7 or primary peritoneal macrophages were washed with FACS buffer containing PBS with 2% FBS and stained with APC anti-mouse TREM-1 Ab (clone: 174021, R&D systems). Unstained cells were used as a negative control to establish the flow cytometer voltage setting ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... pH 7.6] for 2 h at RT and incubated overnight (ON) at 4°C with antibodies against TRKB (1:1000, #AF1494, R&D Systems); AP2M (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... IL-17 was detected using goat anti-mouse IL-17 (1/100 dilution, Catalogue number Af-421-na R&D Systems, UK) followed by biotinylated rabbit anti-goat IgG (1/200 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... of LPS treated rats were double-immunostained for the analysis of the cellular localization of cathepsin X using the following primary antibodies: goat polyclonal anti-cathepsin X antibody (1:75, R&D System), mouse monoclonal anti-TH antibody (1:500 ...