Labshake search
Citations for R&D Systems :
4551 - 4600 of 4704 citations for Recombinant Human IIntercellular Adhesion Molecule 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... The matured THP-1 cells were not treated or treated with 20 μg/mL poly (I:C) (4287, R&D Systems, Minneapolis, MN) for four additional hours before the cells were collected for analysis.
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated in the following primary antibodies overnight at 4L: IL-17B (1:100, Cat #1248, RRID:AB_354696, R&D Systems, Minneapolis, MN), TNF-alpha (1:100 Cat #AF-410 ...
-
bioRxiv - Immunology 2023Quote: ... Total TGF-β levels were determined by acid activation of the latent TGF-β1 in the sample using the sample activation kit 1 (DY010) (R&D Systems).
-
bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
-
bioRxiv - Bioengineering 2023Quote: ... MVN and tumoroid treatments were administered over 4 days through daily media perfusion with an IL8 blocking antibody (20 µg mL-1, #MAB208, R&D Systems), Dexamethasone (5 µM ...
-
bioRxiv - Systems Biology 2023Quote: ... primary RTK antibodies were used at a 1:1000 concentration followed by anti-rabbit or -mouse-HRP (R&D Systems #HAF008, #HAF007). Primary antibodies p-ErbB2 (MilliporeSigma #04-293 ...
-
bioRxiv - Cell Biology 2023Quote: ... The remaining volume was divided into two tubes and incubated on ice with either 1 μg of anti-galectin-9 (AF2545, R&D systems) or goat isotype control (Jackson Immunoresearch ...
-
bioRxiv - Biochemistry 2023Quote: ... The samples were then washed with FACS buffer and labeled with 1 µl/well of phycoerythrin-conjugated anti-mouse secondary (F0102B, R&D Systems) 1 hour at 4 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... with primary antibodies at appropriate dilutions (Anti-CD31 from Histonova Cat # DIA-310, diluted 1:100; Anti-podocalyxin from R&D Systems Cat # AF1556 ...
-
Low-dose cytokine immunotherapy of solid cancers enabled by phagocytic-competent protein co-crystalsbioRxiv - Cancer Biology 2023Quote: ... 24 and 48 hours intervals from 3 BALB/C mice carrying RCC tumours receiving 1×107 PODS-IL-2 treatment following the final dose and tested by IL-2 ELISA (R&D Systems) according to the manufacturer’s protocol.
-
bioRxiv - Immunology 2023Quote: ... followed by incubation with an anti-goat IgG horseradish peroxidase-conjugated secondary antibody at a 1:1000 dilution (HAF107; R&D Systems). As a control ...
-
bioRxiv - Bioengineering 2023Quote: ... Microwells were then stained for 4 hrs with 10 µg mL-1 of donkey anti-sheep IgG NL493-conjugated antibody (NL012, R&D Systems) and 10 µg mL-1 donkey anti-rabbit IgG Alexa Fluor 555-conjugated antibody (ab150062 ...
-
bioRxiv - Neuroscience 2023Quote: ... Neuropili-1 inhibition studies were repeated as described above for EG00229 using a goat polyclonal anti-NRP-1 blocking antibody (AF566; R&D Systems) at 1 µg/mL ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was thoroughly washed with PBS containing 0.05 % Tween-20 (0.05 % PBS-T) and then incubated for 1 h at room temperature with Anti-Mouse IgG HRP-conjugated antibodies (R&D Systems), diluted 1:1000 in the blocking buffer ...
-
bioRxiv - Microbiology 2023Quote: The levels of mouse TNF-α and IL-1β were quantified from the supernatant of homogenised skin samples using Mouse TNF-alpha DuoSet ELISA (DY410) or Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401) (R&D Systems) following the manufacturer’s protocols ...
-
bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no. 39649, lot 02617020, used 1:250; SOX2, R&D Systems, AF2018 ...
-
bioRxiv - Cell Biology 2023Quote: ... Cytokine levels in BMEF were then measured using mouse CXCL12/SDF-1 alpha (MCX120) and SCF (MCK00) Quantikine ELISA kits (R&D Systems) according to the manufacturer’s protocols.
-
bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used in this study: Goat IgG against VE-cadherin (1:100, AF1002, R&D Systems, Minneapolis, MN), pY658 and pY685 VEC (1:40 ...
-
bioRxiv - Genetics 2024Quote: ... membranes were incubated with horseradish peroxidase⍰conjugated secondary antibody (hCD55 and hCD39, rabbit IgG, 1:5000 (Cat. no. HaF008, R&D Systems); hTBM and β-actin ...
-
bioRxiv - Microbiology 2024Quote: ... or N protein of SARS-CoV-2 (dilution 1:2000) or SARS-CoV-2 membrane ab (R&D Systems, Cat no. MAB10690). Followed 3 time washing with PBST (PBS with 0.1 tween 20 ...
-
bioRxiv - Cell Biology 2024Quote: ... the negative lentiviral status was confirmed for each cell line using the HIV-1 p24 DuoSet ELISA rom kit (DY7360-05, R&D Systems).
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies at 4 °C overnight (14-20 h) at the following dilutions: anti-PDGFRα (AF1062-SP, goat IgG, 1:250; R&D Systems), anti-APC (Ab-7 ...
-
bioRxiv - Biochemistry 2024Quote: ... Secondary incubation was 1 h at ambient temperature in 5% skim milk with mouse or rabbit IgG horseradish peroxidase-conjugated antibodies (R&D Systems, MN ...
-
bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910), or MCP-1 (MAB679, clone 23007) obtained from R&D Systems. For patient sample analysis using the immunostorm chip 10 µL of diluted human plasma (1:10 dilution with 1x PBS ...
-
bioRxiv - Microbiology 2024Quote: ... polyclonal), IL-6 (MAB9540, clone 973132), IL-12p70 (MAB611R, clone 24945R), and MCP-1 (AF-279, polyclonal) obtained from R&D Systems. Surface enhanced Raman scattering (SERS ...
-
bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
-
bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with the first primary antibody (an anti-IL-1R1 antibody, 1: 100, MAB269, R&D Systems, Minneapolis, USA). Negative controls were incubated in the same solution without the primary antibody ...
-
bioRxiv - Microbiology 2021Quote: ... in PBS for 1LJh and then stained overnight at 4°C with a primary anti-SP-D antibody (1:500, R&D Systems, AF1920), diluted in PBS with 0.5% BSA and 0.5% Triton X-100 ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were stained with ITGB4 antibody conjugated to Alexa Fluor® 488 (5 μl/1 million cells) (R&D Systems, Minneapolis, MN, USA) and Propidium Iodide Ready Flow™ Reagent (1 drop/1 million cells ...
-
bioRxiv - Bioengineering 2021Quote: ... 36 million cells were used for sorting with the Sertoli cell marker Thy-1 Cell Surface Antigen (THY1/CD90) using a PE-conjugated antibody for THY1/CD90 (R&D Systems, FAB2067P) and the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
-
bioRxiv - Neuroscience 2020Quote: ... immunosorbent 96-well plate was coated overnight at +4°C with 1 μg/ml of goat anti-mouse CDNF (R&D Systems, AF5187) in coating buffer (15mM sodium carbonate ...
-
bioRxiv - Microbiology 2021Quote: ... cells were washed with FACS buffer (1x PBS supplemented with 2% FBS and 1 mM EDTA) and stained with goat anti-ACE2 (R&D Systems, #AF933) at a 1:50 dilution or rabbit anti-LRRC15 (abcam ...
-
bioRxiv - Cancer Biology 2022Quote: ... 500 000 cells were stained for 20 min at 4°C in the dark with the APC-conjugated anti-P-cad antibody (1:10; R&D system, FAB861A) and the Live/DeadTM Fixable Violet Dead Cell Stain Kit (1:1000 ...
-
bioRxiv - Microbiology 2022Quote: ... PBS was removed and replaced with 200 µL of blocking solution (dPBS with 2% (v/v) BSA) containing α-TIM1(1:100, AF1750, R&D Systems) or α - AXL antibody (1:100 ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were washed 5 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of IL-1β conjugate (R&D Systems, UK). Plates were washed and incubated at room temperature for 1 hour with horseradish peroxidase-conjugated streptavidin (R&D Systems ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times with wash buffer and then incubated for 1 hour at room temperature with 200 ul of detection antibody (R&D Systems, UK). Plates were washed and incubated at room temperature for 30 minutes with horseradish peroxidase-conjugated streptavidin (strep-HRP ...
-
bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
-
bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
-
bioRxiv - Neuroscience 2019Quote: ... A 1:6 series of free-floating tissue was stained for TH (MAB318, MilliporeSigma, Burlington, MA) Nurr1 (AF2156, R&D Systems, Minneapolis, MN) or GFP (AB290 ...
-
bioRxiv - Immunology 2020Quote: ... and then brought to 1-2 million PBMCs/mL in R10 buffer plus 500pg/mL rhIL-7 (R&D Systems, Minneapolis, MN, USA) and rested again for four days ...
-
bioRxiv - Cell Biology 2019Quote: ... 2 ug/ml Doxycycline and 3.34 ug/ml Puromycin, 1 × N27 supplement, and 10 ng/ml BDNF (STEMCELL, 78005) and GDNF (R&D Systems, 212-GD)) ...
-
bioRxiv - Physiology 2020Quote: ... 0.1 mL/10 g body weight) on day 1 and leptin (2 mg/kg; 0.1 mL/10 g body weight; CAT# 498-OB, R&D Systems, Minneapolis, MN) on day 2 at 6am ...
-
bioRxiv - Neuroscience 2019Quote: ... were cut on a cryostat and mounted on slides and processed for GDNF receptor GFRα1 (goat anti-GFRα1, 1:100; cat no. AF-560, R&D Systems, Minneapolis, MN, USA) and P2X5 (rabbit anti-P2X5 ...
-
bioRxiv - Cell Biology 2021Quote: ... HTM cells were cultured in serum-free DMEM with 1% PSG and subjected to the different treatments for 3 d: TGFβ2 (2.5 ng/ml; R&D Systems, Minneapolis, MN, USA), the ERK inhibitor U0126 (10 μM ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...
-
bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed 3x in TBST and incubated for 1 h with anti-CatL polyclonal IgG (R&D Systems, AF952-SP) diluted 1:1,000 in TBST followed by 30 min with rabbit anti-goat HRP IgG (SouthernBiotech ...
-
bioRxiv - Immunology 2021Quote: ... Primary antibody (Anti-mouse RAE-1 pan-specific antibody, recognizing RAE-1α, β, δ, γ and ε (R&D Systems, Catalogue Number AF1136) or an appropriate isotype-matched control (polyclonal goat isotype IgG ...
-
bioRxiv - Pathology 2022Quote: ... was seeded with 10 000 myoblasts in serum-free medium while the wells were filled with either proliferating medium or serum-free medium complemented with 10 ng.mL-1 FGF-b (R&D Systems 3139-FB), IGF-I (R&D Systems 791-MG ...
-
bioRxiv - Genomics 2022Quote: Circulating AAV-expressed human ANGPTL3 protein levels were measured from plasma using commercially-available ELISA kits (plasma diluted 1:4 and used with R&D Systems #DANL30). The assay is specific for detection of human ANGPTL3 protein ...
-
bioRxiv - Molecular Biology 2022Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...