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Citations for R&D Systems :
4001 - 4050 of 4069 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Seven different ligand conditions were tested at concentrations previously determined to elicit maximal cell responses49 (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM) ...
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bioRxiv - Neuroscience 2024Quote: ... double-coated with PLO as described above followed by a second coating with fibronectin (5µg/ml, incubation overnight at 37 °C; R&D Systems, Minneapolis, MN, USA) and once washing with PBS.
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bioRxiv - Immunology 2024Quote: ... Lymphocytes from SP and draining LNs were cultured in the presence of IRBP-p (10 μg/ml) and IL-23 (R&D systems, Minneapolis, MN) (10 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Cell Biology 2024Quote: ... isolated acinar clusters were resuspended in a stiff matrix composed by 1:1:1 mix of Matrigel-GF reduced : rat collagen I neutralized solution 4.43 ng/mL (R&D Systems – 3440-100-01) ...
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bioRxiv - Genomics 2024Quote: FACS-sorted Vcam1-FAPs were grown to 60-80% confluence followed by adipogenic differentiation and were treated with 500 ng/mL recombinant mouse sFRP-1 protein (R&D systems, 9019-SF) with or without sFRP-1 inhibitor ...
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bioRxiv - Developmental Biology 2024Quote: ... Agents were added to the medium at final concentrations of 1 μg/mL for BMP4 treatment (314-BP; R&D Systems, Minneapolis, MN, USA), 1% v/v of 4 mM HCl containing 0.1% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... For differentiation into quiescent astrocytes adherent NPC cultures were washed twice with N2B27 and cultured for at least 48h with N2B27+ 10ng/mL BMP4 (R&D Systems 314-BP-010).
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bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse SCLC cells were seeded into 6-well plates and cultured for 16 h in RPMI1640 + 10% BGS with or without 100 ng/mL of Wnt5a (R&D System, 645-WN-010) or recombinant Wnt3a (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Immunology 2019Quote: ... The B cells were then added to the APCs and cultured in complete medium with 5 ng/ml BAFF (R&D Systems, #8876-BF-010) and 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Physiology 2019Quote: ... Differentiation of M-CSF-dependent bone marrow osteoclast precursors was induced with 30 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL) (R&D Systems, Minneapolis, MN, USA) and 30 ng/mL M-CSF for 4 days ...
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bioRxiv - Cell Biology 2021Quote: ... For differentiation assays cells were cultured in differentiation media (DMEM + 1% FBS + 1% P/S) with or without recombinant TGFβ (R&D systems, 10 ng/mL) and analyzed 72 hours later by immunofluorescent staining described below ...
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bioRxiv - Developmental Biology 2021Quote: ... Organoids were subsequently differentiated towards cartilage by culturing them in the presence of 20 ng/ml BMP4 (R&D Systems, cat. no. 314-BP-010) for 6 days.
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bioRxiv - Cancer Biology 2021Quote: ... the culture media were replaced with 1 ml fresh media supplemented with 10% FBS and the designated concentration of the recombinant mouse IFNγ (R&D Systems, 485-MI-100) or recombinant human IFNγ (R&D Systems ...
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bioRxiv - Neuroscience 2022Quote: ... a complete media change was performed with CDMIII containing respective compounds at a final concentration of 8.8 ng/ml (422.6 nM) IL-6 (R&D Systems, Cat. no. 7270-IL-025) and 25 ng/ml (422.6 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... Transiently transfected Jurkat cells in a volume of 500 μL were incubated in the absence or presence of the activators: TNF-α (20 ng/mL; R&D Systems, Minneapolis, MN), Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Developmental Biology 2022Quote: ... day 5 lateral plate mesoderm or day 7 differentiating lung mesenchyme was transferred to cSFDM supplemented with 100 ng/ml rmVEGF (R&D Systems, # 493-MV-005). For evaluation of differentiation efficiency ...
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bioRxiv - Genomics 2024Quote: ... The human RPTEC with early passages were seeded at 8 × 105 cells on 10 cm culture dish at the day before the assay and treated with or without TNFα (R&D systems, 100 ng/ml) at 3 h before the fixation ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
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bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed and blocked in PBS with 2% donkey serum and 2% bovine serum albumin for an hour and incubated with rabbit anti-IBA1 (WAKO, cat#019-19741, RRID:AB_839504, 1:500) and goat anti-GPNMB (R&D Systems, cat#AF2550, RRID:AB_416615, 3µg/ml) primary antibodies in PBS with 2% donkey serum overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: Reprogramming was induced in the presence or absence of IFNγ at 10 ng/ml (from day 0 to 5) (R&D Systems, 485-MI-100). SSEA1+ day 5 iPSCs (4 replicates from different reprogramming rounds ...
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bioRxiv - Biochemistry 2023Quote: FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/ml rhIL-17A (R&D Systems, #7955-IL-025/CF), rhIL-17B (R&D Systems ...
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bioRxiv - Systems Biology 2023Quote: ... The assays with TNF pre-conditioning were performed identically except that HBMECs were activated with 10 ng/ml TNF (R&D Systems Cat# 10291-TA) for ∼22 hours before media equilibration ...
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bioRxiv - Neuroscience 2023Quote: ... CD8 T cells were activated with immunoCult Human CD3/CD28/CD2 T Cell Activator beads (cat. #10970) in co-culture with NK cells in the presence of IL2 (2ng/mL) (R&D Systems cat. #BT-002) and IL15 (0.1ng/mL ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Bioengineering 2023Quote: ... They were then perfused through the renal vein at 100 mL/min with a pressure less than 20 mmHg in custom antibiotic-free endothelial media containing endothelial basemedia (R&D Systems, Minneapolis, MN, #CUST01707), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and the signalling ligands identified from the ligand-receptor analysis (Figure 4C): murine WNT5A (250 ng mL−1, R&D Systems 645-WN-010/CF), murine SEMA3A (250 ng mL−1 ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fibroblasts at 70-80 % confluent were serum starved overnight and treated with 50ng/ml of recombinant human PDGF-DD (R&D system, 1159-SB-025) for 24h.
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bioRxiv - Cancer Biology 2023Quote: ... in combination with different concentrations of inhibitor of IFNα/β (i-IFNα/β) (Recombinant Viral B18R, R&D Systems; 0.9, 1.8 or 3.6 ng/ml) for 40 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... EV samples at a concentration of 1 × 1010 particles/mL were incubated with AF647-labelled anti-TSPAN2 antibody (R&D systems, FAB7876R-100UG, 0.5 µL) or a mixture of APC-labelled anti-CD9 (Miltenyi Biotech ...
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bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Bioengineering 2023Quote: RFP-MDA-MD-231 and GFP-HUVEC at 3 million/mL and 8 million/mL in precursor solution were photopatterned and perfused using the peristaltic pump previously mentioned at 0.2 rpm/mL for five days before tumor necrosis factor-alpha (TNF-α) (R&D Systems, Minneapolis, MN, USA) and Vascular endothelial growth factor (VEGF ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Immunology 2024Quote: ... mice with 20% L929-conditioned medium (LCCM) in DMEM and 20ng/mL recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems, Minneapolis, MN, USA) in RPMI for BMDM and BMDC ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture plates for cell invasion assays were prepared by adding 150 μL of 1 mg/mL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3433-005-R1) to a 24-well cell culture insert with an 8 μm pore size (Falcon ...
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bioRxiv - Bioengineering 2024Quote: ... Maturation of hepatic endoderm was initiated on differentiation days 16 ─ 21 using complete HCM media supplemented with 10ng/ml Oncostatin M (OSM: R&D systems, # 8475-OM-050), 20ng/ml HGF and 0.1μM Dexamethasone (ThermoFisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed twice with PBS and further cultured in N2B27 medium supplemented with 20 ng/ml bFGF (R&D Systems, cat. 233-FB-025/CF) and 20 ng/ml Activin A (PeproTech ...