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Citations for R&D Systems :
3801 - 3850 of 4069 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The tri-culture medium consisted of supplementing the co-culture medium with 100 ng/mL mouse IL-34 (R&D Systems), 2 ng/mL TGF-β (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... A4403], 125 µM db-cAMP [Tocris, 1141], 2.5 µM DAPT [Tocris, 2634], 10 ng/mL each of BDNF [R&D Systems, 248-BD-025] ...
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bioRxiv - Immunology 2023Quote: ... K562 cells were stimulated in complete RPMI media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems), 20 ng/mL human IFN-β (84-99I-F010 ...
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bioRxiv - Immunology 2023Quote: BMDMs were stimulated in complete αMEM with the following proteins as indicated: 20ng/mL mouse IFN-ψ (485MI100, R&D Systems), 20ng/mL mouse IFN-β (8234MB010 ...
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bioRxiv - Immunology 2023Quote: ... HUT78 cells were stimulated in complete IMDM media with 20 ng/mL human IFN-ψ (285-IF-100, R&D Systems). All treatments were 24 hours.
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bioRxiv - Biochemistry 2022Quote: ... The cell layers were incubated for 1 hour with 10 µg per ml monoclonal anti-His6-tag antibody (R&D Systems) or a 1:1000 dilution of pAb 9543 279 anti-fibrillin-1 ...
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bioRxiv - Cell Biology 2023Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... United States) or equine TNF-α (1 ng/mL; catalog no. 1814-ET-025/CF, R&D Systems, Minneapolis, United States) in combination with LPS ...
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bioRxiv - Cell Biology 2023Quote: ... the medium was replaced with StemPro-34 SFM supplemented with 50ng/mL VEGF-165 and 8μM SB431542 (Cat. 1614/10, R&D systems). From Day12 onwards ...
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bioRxiv - Cell Biology 2023Quote: Isolated CD8+ DCs were cultured in R10 media supplemented with recombinant mouse FLT3L (250ng/ml; R&D Systems, 427-FL-025). CD8+ DCs isolated from Rubicon-proficient mice were incubated in the presence or absence of 5μM Fendiline-HCl (Tocris ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 mg/ml of purified recombinant human complement component C5a (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... melanocytes were suspended in 1 ml medium and seeded on 0.5% collagen (Cultrex, R&D Systems, Minneapolis, MN, Cat # 3442-050-01), in 24 well plates for three days.
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bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
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bioRxiv - Cell Biology 2020Quote: ... mOSE cells (1×10^6) were first plated as monolayer cultures 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). Four days after the addition of TGFB1 ...
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bioRxiv - Microbiology 2020Quote: ... Fully-differentiated MDM were generated by culturing MC under adherent conditions for seven days using RPMI-AS medium supplemented with 10 ng/mL M-CSF (R&D Systems). T cells were purified from PBMC using T cell pan isolation kit (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were converted to an epiblast-like (EpiLC) state by 48 hour culture in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeded at 6×105/cm2 or 3×105/cm2 (corresponding to high or low cell density) on 24-well transwell plates in MEF-CM supplemented with 20 ng/ml bFGF (R&D Systems), 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Developmental Biology 2021Quote: ... 8×105 cells were seeded on each coverslip in a defined volume of MEF-CM supplemented with 20ng/ml bFGF (R&D Systems), 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Bioengineering 2022Quote: ... jam-packed microgels in the pellet were mixed with collagen solution (5 mg mL-1 Cultrex rat collagen I, R&D Systems) in 2:1 ratio ...
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bioRxiv - Cancer Biology 2022Quote: CAR and BOXR1030 T cells were activated with immobilized recombinant GPC3 protein (0.3 mg/mL; R&D Systems, Cat# 2119-GP-050) for 4 and 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Immunology 2021Quote: ... This reaction was carried out by incubating 1 μM of the quenched AZP probe with 1 μg/mL of recombinant MMP14 (918-MP-010, R&D Systems), ADAM17 (2978-AD-10 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells incubated at 37°C/5%CO2 for approximately 1hr prior to flooding the dishes with F12 complete media alone or complete F12 media containing 100ng/mL GDNF (R&D Systems). Cells incubated overnight at 37°C/5%CO2 prior to being rinsed in PB and fixed in 3% paraformaldehyde ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Immunology 2019Quote: ... 96-well flat bottom plates were pre-coated with 20 μg/ml of an agonist anti-TREM-1 mAb (clone 174031; R&D Systems) overnight at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then treated with 200ng/mL of recombinant rat NogoA (1026-1090aa) and Fc Chimera protein (Nogo-66; R&D Systems), or with human NogoA (566-748aa ...
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bioRxiv - Neuroscience 2020Quote: ... plates were incubated for 1.5 hr at RT with biotinylated sheep anti-mouse TREM2 antibody (0.1 µg/ml, BAF1729, R&D Systems, RRID: AB_356109) diluted in BSA-PBST ...
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bioRxiv - Bioengineering 2020Quote: ... Media for M(IFN-γ) stimulation consisted of RPMI 1640 supplemented with 10 ng/ml interferon-γ (IFN-γ; R&D systems), and media for M(IL4/IL13 ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60-70% confluence were serum starved for 24 h in HITES media and stimulated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and inserts were immersed in 500 µL RPMI media containing 10% FBS in the absence or presence of 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/mL PDGF-AA or PDGF-BB ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were incubated overnight in myogenic growth media (F10, 20% FBS, 1% Pen/Strep; Gibco, supplemented with 5 ng/mL rFGF2; R&D Systems).
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bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Culture was continued for 24 h in low serum medium with 0 or 5 ng/ml TGF-β1 (R&D Systems, www.rndsystems.com) with emodin (0 ...
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bioRxiv - Developmental Biology 2022Quote: ... microglial cells were preincubated with 20µg/ml of anti-C3aR antibody (Catalog # MAB10417) or control IgG (R&D Systems, Inc. Minneapolis, MN) suspended in DMEM/F12 supplemented with 5% heat-inactivated FBS ...
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bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Microbiology 2022Quote: ... followed by three days incubation at 37 °C in the presence of 1 µg/ml of kifunensine (R&D Systems, MN, USA). Supernatant was collected and combined with half volumes of the supernatant of binding buffer (25 mM HEPES ...
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bioRxiv - Immunology 2021Quote: Mature macrophages (n = 6 donors) were stimulated for 48 hours with complete medium containing IFN-γ (20 ng/mL, R&D Systems) and LPS (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fed daily with different cocktails of growth factors and small molecules for twelve days in mesodermal differentiation medium and driven through the anterior primitive streak (1 day; 30 ng/ml Activin [cat. num. 338-AC; R&D Systems, Minneapolis ...
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bioRxiv - Neuroscience 2021Quote: ... The sandwich layout of this TREM2 MSD assay from the bottom to the top is (1) Capture-antibody (1 μg/mL; BAF1729, R&D Systems), (2 ...
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bioRxiv - Biochemistry 2020Quote: In vitro co-precipitation of TREM2 or TREM1 with Aβ oligomers was performed by mixing recombinant Fc-tagged TREM2 or TREM1 ectodomain (residues 19-171; 100 ng/ml, R&D systems), 100 nM HiLyte Fluor 647-labelled Aβ oligomers and Protein G Dynabeads in 0.5% fatty acid free BSA PBS-Tween buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Microbiology 2022Quote: ... Using density gradient medium (Lymphoprep) monocytes were isolated and differentiated into macrophages in the presence of GM-CSF (100 ng/mL) (R&D Systems) in 1640 RPMI + 5% FBS +P/S media for 6 to 7 days
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bioRxiv - Cell Biology 2022Quote: ... freshly isolated RBCs in basal medium supplemented with 10 mM HEPES at 20% hematocrit were incubated for 30 minutes under 37 °C with gentle shaking with 5 μg/mL ADAMTS13 (R&D Systems) [27 ...
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bioRxiv - Microbiology 2022Quote: ... cell media was replaced with media containing the compound and 20 ng/mL tumor necrosis factor alpha TNF-α (R&D Systems).