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Citations for R&D Systems :
3851 - 3900 of 4069 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... 5 × 105 cells in 100 μl adhesion buffer were added to the upper chamber and either 500 ng/ml CCL2 (R&D Systems) or 10 nM C5a (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
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bioRxiv - Developmental Biology 2020Quote: ... state through prolonged culture (more than 5 passages) in N2B27 medium containing 12 ng/ml FGF2 (233-FB-025, R&D Systems) and 20 ng/ml ACTIVIN A (120-14P ...
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bioRxiv - Developmental Biology 2021Quote: ... were cultured on gelatine and FCS-coated plates in N2B27 medium supplemented with recombinant 20 ng ml–1activin A (338-AC-050, R&D Systems) and 2 μM IWP2 (3533 ...
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bioRxiv - Developmental Biology 2021Quote: ... accutased naive ESCs were seeded onto gelatine and FBS-coated plates in N2B27 medium supplemented with recombinant 20 ng ml–1activin A (338-AC-050, R&D Systems) and 12 ng ml–1 bFGF (100-18B ...
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bioRxiv - Cancer Biology 2021Quote: MDA231-LM2 cells in DMEM with 10% FBS were cultured with or without incremental dosages (10-200 ng/ml) of recombinant OPG (R&D Systems) for 30 min ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Genetics 2020Quote: ... cells were grown until colonies were visible and subsequently passaged at low densities on Biolaminin 521 LN® (Biolamina) coated 24 well plates supplemented with 1.5 µg/mL E-Cadherin (R&D systems) to obtain single cell colonies ...
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bioRxiv - Genomics 2021Quote: ... Isolated B cells were cultured in IMDM medium supplemented with 10% FBS and 55 mM beta-mercaptoethanol at 1X106 cell/mL and activated by CellXVivo Human B cell expander (1:250 dilution, R&D system) and 50 ng/mL IL2 (Cat#200-02-10ug ...
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bioRxiv - Developmental Biology 2021Quote: ESCs were then differentiated into anteriormost primitive streak through the addition of CDM2 basal medium supplemented with Activin A (100 ng/mL; R&D Systems), CHIR99021 (3 µM ...
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bioRxiv - Developmental Biology 2021Quote: ESCs were differentiated into primitive streak through the addition of CDM2 basal medium supplemented with Activin A (30 ng/mL; R&D Systems), BMP4 (40 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with BMP4 (30 ng/mL; R&D Systems), PD0325901 (100 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were differentiated into anterior primitive streak through the addition of CDM2 basal medium supplemented with Activin A (30 ng/mL; R&D Systems), CHIR99021 (4 μM ...
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bioRxiv - Genomics 2022Quote: Three million cells from each triplicate were seeded separately in 6 well-plates in fresh RPMI media and left unstimulated or stimulated with universal interferon alpha (200U/ml) (R&D systems) for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25-1.25 µg/ml recombinant human rSCUBE3 protein (Cat # 7730-SC, R&D Systems, Abingdon, UK) in the presence of RANKL and M-CSF ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with either M1-activation medium [RPMI160 medium with 20 ng/ml IFNγ (485-MI-100; R&D systems) and 10 ng/ml LPS (L2630 ...
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bioRxiv - Immunology 2020Quote: ... the ears of the mice were intradermally injected with 500 ng recombinant mouse IL-23 (R&D Systems cat. 1887-ML-010) dissolved in 25 μl PBS into one ear and 25 μl PBS into the contralateral ear ...
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bioRxiv - Immunology 2021Quote: ... were coated with anti-IFN-γ antibody (capture mAb; 1 µg/mL) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN). Plates were blocked using culture medium (DMEM containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: LECs were pre-cultured with FBS-free and supplement-free basal medium for 24 h and then treated with medium containing 100 ng/mL of recombinant rat CTGF (R&D Systems) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Molecular Biology 2022Quote: Human dermal fibroblasts transfected with either 10 nM si-circGLIS3 or scrambled siRNA (Eurofins Genomics, Ebersberg, Germany) for 48 hours and treated with 5 ng/ml TGF-β1 (R&D Systems) were rinsed and pelleted in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: The preosteoblast cells cultured in tissue culture plates were differentiated for 5 days and treated with Myostatin of 100 ng/mL (788-G8-010/CF R&D system), follistatin of 100 ng/mL (769-FS-025/CF ...
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bioRxiv - Bioengineering 2022Quote: ... The streptavidin modified nanovial solution was then mixed with a solution containing an antibody cocktail comprising 8 µg/mL anti-mouse CD45 (R&D Systems) biotinylated according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Sorted cells were plated at 5000 cells per well in a 96 well plate and incubated at 37°C overnight in 10 ng/ml IL-7 (R&D Systems) and basal media composed of high glucose DMEM supplemented with non-essential amino acids ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then washed with phosphate-buffered saline (PBS)/0.05% Tween 20 and incubated with mouse IgG2a-anti-IAV NP (Clone Hb65, EVL laboratories) 5 μg/ml or mouse IgG2a isotype control (MAB003, R&D systems) 2.5 μg/ml in PBS/0.1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (including B27 supplement with vitamin A) with 200 ng/ml rmWNT3A (R&D Systems, #1324-WN-010), 100 ng/ml rhFGF2 (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... media was replaced with fresh media containing MCSF and receptor activator of nuclear κB (RANKL) (25 ng/mL each, R&D Systems), and media refreshed every 2-3 days thereafter until a total of seven days stimulation with RANKL ...
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bioRxiv - Cell Biology 2024Quote: ... It was counted as Day 0 when medium was changed to SF medium with 8 ng/mL recombinant Activin A (R&D Systems), 5 ng/mL recombinant human VEGF 165 Protein (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 0.1% FBS (Hyclone Laboratories Inc.)] and stimulated with 10 ng/mL rat PDGF-AA ligand (R&D Systems, Minneapolis, MN, USA) diluted from a 1.5 μg/mL working solution in 40 nM HCl containing 0.1% BSA for the indicated length of time ...
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bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... NK cells were also co-cultured with K562 or P815 coated with anti-NCR (NKp30, NKp44, NKp46, R&D Systems, 5ug/ml) or anti-CD16 (clone 3G8 ...
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bioRxiv - Immunology 2024Quote: ... cells from BM (femora and tibiae) obtained from C57BL/6J mice were plated with 100 ng/ml or recombinant Flt3L (R&D systems) in RPMI1640 medium (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... This medium was aspirated and replaced with complete medium containing 20 μg/ml gentamicin and IFNβ (R&D Systems, 8234-MB-010) as indicated for 20 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed daily for the next three days to DMEM/F12 supplemented with 100 ng/ml recombinant Activin-A (R&D Systems), 100 ng/ml fibroblast growth factor-2 (FGF2 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Immunology 2023Quote: ... 3000 – 5000 ASCs per well were cultured in sterile 96-well tissue culture plates (Falcon) in the presence of 20 ng/mL IL-6 (R&D Systems), 100 ng/mL APRIL (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... Lot #: 413-ML-005) and gremlin 1 (Cat #: 956-GR-050, Lot #: ESD4616121) recombinant proteins were all purchased from R&D Systems. Gusb ...
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bioRxiv - Immunology 2023Quote: ... 25 μl of the supernatant was diluted with 25 μl 1% BSA PBS-T and added to a Nunc Maxisorp plate coated with 1 μg/ml anti-C5a capture antibody (C5a DuoSet ELISA kit, R&D systems). Next ...
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bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were treated with media containing recombinant mouse transforming growth factor-beta 1 (rmTGFβ1) at 5ng/mL (R&D systems, 7666-MB) or normal media ...
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bioRxiv - Cancer Biology 2023Quote: ... Osteoclast differentiation media consisted of standard OCY454 media supplemented with 25ng/ml M-CSF (R&D Systems Catelog # 416-TEC; Lot # ME5021121) and 5 ng/ml RANKL (R&D Systems Catalog #462-TEC-010 ...
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bioRxiv - Immunology 2023Quote: ... The matured THP-1 cells were not treated or treated with 20 μg/mL poly (I:C) (4287, R&D Systems, Minneapolis, MN) for four additional hours before the cells were collected for analysis.
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bioRxiv - Genetics 2023Quote: Reprogramming was done as described previously, combining the IFNγ (10 ng/ml, day 0 to 5) (R&D Systems, 485-MI-100) with the addition of the TET inhibitor molecule Bobcat 339 (concentration of 5 µM ...