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Citations for R&D Systems :
3901 - 3950 of 4069 citations for 3 Methoxy 2 2 4 4 Tetrabde Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days ...
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bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded at 6x104 cells/cm2 and grown for 24 h until they reached 70-80% confluency and following treated with RANKL (30 ng/mL; 462-TR, R&D systems), with or without E2 (10-8 M ...
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bioRxiv - Immunology 2022Quote: Healthy dermal fibroblasts were serum starved for 24 h in DMEM containing 0.5% FBS and stimulated with 10 ng/ml TGF-β (R&D systems), 100 ng/ml Wnt-3a (R&D systems ...
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bioRxiv - Immunology 2023Quote: ... The matured THP-1 cells were not treated or treated with 20 μg/mL poly (I:C) (4287, R&D Systems, Minneapolis, MN) for four additional hours before the cells were collected for analysis.
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS, 1% PS) while freshly prepared media (RPMI, 10%, FBS 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM sodium pyruvate, 1% PS), while freshly prepared media (RPMI, 10% FBS, 1% PS) supplemented with 200 ng/ml MCP-1 (R&D Systems) was added to the bottom chamber ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed daily for the next three days to DMEM/F12 supplemented with 100 ng/ml recombinant Activin-A (R&D Systems), 100 ng/ml fibroblast growth factor-2 (FGF2 ...
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bioRxiv - Biophysics 2023Quote: Collagen fibrils were formed by combining rat tail tendon-derived acid-soluble type I collagen (5 mg/mL in 20 mM acetic acid) (CultrexRat Collagen I, R&D Systems) with 400 mM HEPES + 0.02% sodium azide and 1 M NaCl at room temperature to reach final concentrations of 1 mg/mL collagen ...
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bioRxiv - Molecular Biology 2024Quote: ... From each group 150 ml serum was used for chemokine array using the chemokine Proteome Profiler Array kit (Cat No# ARY020, R&D SYSTEMS) and 200ul was used from each group for cytokine array using the cytokine proteome profiler array kit (Cat No# ARY028 ...
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bioRxiv - Neuroscience 2024Quote: ... SCs were then seeded on poly-L-lysine/laminin coated coverslips or petri dishes and kept in the above-mentioned medium containing recombinant human neuregulin1-ß1/heregulin1-ß1 (0.1 mg/ml, R&D systems, 396HB) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 24 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Cell Biology 2024Quote: ... cultures were exposed for the duration of treatment with either 100ng/mL recombinant mouse Sfrp2 (R&D Systems Cat 1169-FR-025) or vehicle control (PBS with 0.1% BSA).
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
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bioRxiv - Immunology 2024Quote: ... In vitro TNFα-blocking studies were performed using recombinant human TNFRII/TNFRSF1B-Fc chimera protein (1 µg / ml) (726-R2-050, R&D Systems) for 18 h.
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice ...
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bioRxiv - Evolutionary Biology 2020Quote: ... to evacuate all mTeSR1 and then were treated for 24 hours with Activin A (100 ng/mL, R&D Systems, 338-AC-010), CHIR99021 (2 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... media in the wells was either with or without 10% FBS and 1μg/mL secreted N-SLIT2 (R&D Systems, 8616-SL-050). The cells were incubated in the Matrigel-coated inserts for overnight in the incubator ...
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bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested using the Quantikine human IL-6 (sensitivity < 5 pg/mL) ELISA kit (R&D Systems, Inc., Minneapolis, MN) according to manufacturer’s recommended conditions ...
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bioRxiv - Neuroscience 2019Quote: ... MSD GOLD 96-Well Streptavidin plates were blocked with 3% BSA and coated with a solution containing 0.25 µg/ml biotinylated polyclonal goat anti-human TREM2 capture antibody (BAF1828, R&D Systems, Minneapolis, MN). CSF and plasma samples diluted 1:4 in 1% BSA were added to the prepared plates and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... 100 µl of supernatant samples and standards (recombinant mouse TREM2-FC, 1729-T2; R&D Systems; 0-20 ng/ml in BSA-PBST) were then incubated for 2 hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Spain) and supplemented with each one of the following combinations of growth factors: (i) 100 ng/ml Act A (R&D Systems, UK), (ii ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies have been used in this study: Anti-Human/Mouse/Rat/Chicken Pax7 (5 μg/ml, R&D Systems, MAB1675), Anti-Human/Mouse Myod1 (5.8A ...
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bioRxiv - Bioengineering 2021Quote: ... Stimulation was carried out in 96 well plates pre-coated with DLL4 and VCAM1 as described above in 100µL per well late T cell differentiation media supplemented with 20ng/mL IL-21 (R&D Systems, 8879-IL) and 1.25% ImmunoCult Human CD3/CD28/CD2 T cell activator (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... For IFN pre-stimulation, 200 IU/ml IFN-β (IFN-β1, Bioferon, Laupheim, Germany) or IFN-γ (R&D Systems, Minneapolis, MN, USA) were added at the time of seeding ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: For pro-fibrogenic mediator studies control lung fibroblasts were treated in the presence of EGF (R&D systems, 236-GMP-200, 10ng/mL), TGFβ1 (R&D systems ...
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bioRxiv - Cancer Biology 2022Quote: ... bioreactor media were either left untreated or supplemented with anti– Siglec-9 antibody (5 μg mL-1; clone 191240, #MAB1139-500, R&D systems, USA). On day 5 ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... containing 10% fetal bovine serum and 0.5% gentamicin in the presence of 1000 IU/ml of human granulocyte-macrophage colony-stimulating factor (GM-CSF; R&D Systems, Minneapolis, MN) and 1000 IU/ml of interleukin 4 (IL-4 ...
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bioRxiv - Pathology 2020Quote: ... were transduced into another line of HK-2 cells in the same manner as that of LOXL2 shRNA particles and were further incubated with media containing either TGF-β (20 ng/ml, R&D Systems) or vehicle (0.1% 4mM HCl/BSA ...
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bioRxiv - Neuroscience 2020Quote: ... in culture medium with recombinant mouse TNF-α (10 ng/ml from a stock solution of 100 μg/ml made up in 0.2 % bovine serum albumin and sterile PBS, R&D systems, aa-80325) to stimulate release of inflammatory mediators (TNF-FLS ...
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bioRxiv - Pathology 2020Quote: ... Cells were cultured to 70-80% confluence and then treated with vehicle (4mM HCl with 0.1% bovine serum albumin) or 10 ng/mL TGF-β1 (Cat#: 7754-BH-005, R&D Systems, Minneapolis, MN) or 10 ng/mL TNF-α (Cat#210-TA-020 ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated in 0.5% serum-containing media before treatment with 200 ng/mL recombinant Shh N-terminal peptide (R&D Systems, 1314-SH) diluted in dimethyl formamide (DMF).
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bioRxiv - Microbiology 2020Quote: ... The cells were then seeded onto 24 well plates (106 cells/well) with or without 10 ng/mL murine IFN-γ (R&D Systems). Sixteen hours later ...
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bioRxiv - Immunology 2020Quote: ... except for the STORM experiments where they were used at a concentration of 20 μg/ml: α-MerTK (mouse extracellular monoclonal, 125618, R&D Systems), α-MerTK (goat extracellular polyclonal ...
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bioRxiv - Microbiology 2021Quote: ... LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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bioRxiv - Cancer Biology 2022Quote: ... as previously described.30 The concentrations of function blocking antibodies used were: 1 μg mL−1 Anti-laminin 5 antibody (R&D systems, #MAB2144), 1 μg mL−1 α3 anti-integrin antibody ...
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bioRxiv - Cell Biology 2022Quote: ... primary BMSCs were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 72 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD) and Wnt3a (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... primary BMSCs isolated from C57BL/6 mice were plated in 6-well dishes until 80% confluent and serum starved (0.5% FBS) for 48 hours prior to treatment with one or a combination of CXCL12 (200 ng/ml, R&D Systems, 460-SD), Wnt3a (100 ng/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were cultivated in neural progenitor medium based on 2D-NIM medium without the dual SMAD inhibitors SB-431542 and LDN-193189 and supplemented with 20 ng/mL recombinant human basic FGF (#233-FB, R&D Systems, Germany) and 10 µM Y-27632 (only for the first 24 h after passaging) ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were stimulated in non-tissue culture treated plates with 100ng/mL GM-CSF (R&D Systems, Cat# 7954-GM-020/CF) and IL-4 (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were allowed to recover for 5 min and were treated with 10 ng/ml PDGF-AA (PDGFRα homodimer cells) or PDGF-BB (PDGFRα/β heterodimer and PDGFRβ homodimer cells) ligand (R&D Systems) for the indicated amount of time ...
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bioRxiv - Cell Biology 2023Quote: ... PDGFRα homodimer cells and PDGFRβ homodimer cells at ∼60–70% confluence or PDGFRα/β heterodimer cells at ∼70-80% confluence were serum starved for 24 h in HITES medium and stimulated with 10 ng/ml PDGF-AA (PDGFRα homodimer cells) or PDGF-BB (PDGFRα/β heterodimer and PDGFRβ homodimer cells) ligand (R&D Systems) for the indicated length of time ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were cultured in N2B27 with 10 ng/ml mouse basic fibroblast growth factor (bFGF) recombinant proteins (R&D systems #313FB025/CF). On Day 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Neuroscience 2024Quote: ... MaxiSORP 96-well plates were coated with 1μg/ml of a rat anti-mouse/human TREM2 monoclonal antibody (R&D Systems; clone 237920) at 4°C ...