Labshake search
Citations for R&D Systems :
1901 - 1950 of 2461 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Site specific phosphorylation levels of 43 kinases were measured using the Human Phospho-Kinase Array Kit (catalog no. ARY 003B, R&D Systems) according to the manufacturer’s instructions with the experimental design as described below ...
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bioRxiv - Physiology 2020Quote: ... and probed with either an affinity-purified antibody fraction of mouse antiserum to a synthetic peptide of human cystatin-s corresponding to amino acid residues 21-141 (AF1296, R&D Systems) or an affinity-purified goat antibody raised against a peptide mapping at the C-terminus of human amylase (sc-12821 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteome Profiler Human Apoptosis Array kit (CAT# ARY009) and Pepstatin A aspartic protease inhibitor (#1190/10) were purchased from R&D Systems. The cOmplete protease inhibitor cocktail (CAT# 11697498001 ...
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bioRxiv - Cell Biology 2021Quote: Isolated human platelets were incubated at room temperature for 15-20 minutes with either recombinant HMGB1 (0-40 μg/ml; R&D Systems), ADP (0-5 μM ...
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bioRxiv - Cell Biology 2020Quote: ... For TGF-β1 detection in condition media from HPC treated with different experimental conditions and in urine samples from mice and human were analyzed by TGF-β1 ELISA commercial kit (R&D Systems) according to the man-ufacturer’s protocol.
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... The following antibodies were purchased and used for immunostaining of human sample using staining protocols similar to those used in mouse sections: CD31 (AF806, R&D systems), CXCR4 (2B11 ...
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bioRxiv - Developmental Biology 2019Quote: ... Human iPSC generated in this study were characterized for their differentiation potential into the three germ layers using Human Three Germ Layer Kit (R&D systems),according to the manufactures’ instructions with minor modifications ...
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bioRxiv - Immunology 2020Quote: Titration of blocking antibodies was performed by using static adhesion assays in which activated CD4+ T cells were allowed to adhere to either recombinant human ICAM/Fc or VCAM/Fc chimeras in the presence of blocking antibodies specific for integrin α4 (clone 2B4, R&D Systems) or integrin αL (clone TS1/22 ...
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bioRxiv - Cell Biology 2019Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed with 1× Phosphate buffered saline (PBS) and stimulated with 1000 U/mL of human recombinant IFNβ (R&D Systems) in DMEM for the designated length of time ...
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bioRxiv - Cell Biology 2019Quote: ... Cxcl10 and Ccl22 in mouse peritoneal cells were analyzed by ELISA according to the manufacturers instructions (Human and Mouse DuoSet ELISA respectively, R&D Systems). For relative chemokine concentration in comparison to parasite load ...
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bioRxiv - Immunology 2020Quote: ... Cells were re-seeded onto 24-well plates at 1 × 105 cells/well and polarized for 48 hr with the addition of 50 ng/ml human IFNγ (R&D systems) for M1 macrophages ...
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bioRxiv - Biochemistry 2019Quote: The corneal micropocket assay was performed as described16 using pellets containing 80 ng of basic fibroblast growth factor (bFGF) or 180 ng of human carrier-free recombinant vascular endothelial growth factor (VEGF; R&D Systems) in C57BL/6J mice ...
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bioRxiv - Cell Biology 2020Quote: ... and chondrogenic differentiation experiments were performed following the instructions of the human mesenchymal stem cell functional identification kit (R&D systems, SC006). For osteogenic differentiation ...
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bioRxiv - Microbiology 2021Quote: ... were isolated from whole blood (Washington National Primate Research Center) from two independent donors using human erythrocyte lysing kit (R&D Systems) and 95% Lymphoprep (Stemcell Technologies ...
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bioRxiv - Genomics 2021Quote: ... Isolated B cells were cultured in IMDM medium supplemented with 10% FBS and 55 mM beta-mercaptoethanol at 1X106 cell/mL and activated by CellXVivo Human B cell expander (1:250 dilution, R&D system) and 50 ng/mL IL2 (Cat#200-02-10ug ...
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Kinetics Of Interferon-λ And Receptor Expression In Response To In Vitro Respiratory Viral InfectionbioRxiv - Immunology 2021Quote: ... Lambda IFN levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). IL-6 was measured by human IL-6 DuoSet ELISA (DY206 ...
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bioRxiv - Cancer Biology 2021Quote: Effects of rSCUBE3 were investigated by treatment of osteoclast cultures with 0.25-1.25 µg/ml recombinant human rSCUBE3 protein (Cat # 7730-SC, R&D Systems, Abingdon, UK) in the presence of RANKL and M-CSF ...
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bioRxiv - Developmental Biology 2020Quote: ... BMP4 + Activin A treatment was accomplished by treating cells with mTeSR supplemented with 3 ng/mL recombinant human BMP4 protein (R&D Systems) and 100 ng/mL recombinant human Activin A protein (R&D Systems) ...
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bioRxiv - Microbiology 2021Quote: ... Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175) antibody (catalog no. MAB835; R&D Systems, Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... 5×106 PBMCs were combined with 5×106 irradiated BLCLs from MHC-identical donor MCMs in 5 ml of RPMI-1640 containing 15% FCS (R15) and 10 ng/ml recombinant human IL-7 (R&D Systems). Cultures were supplemented 2 days later with 1.25 ml of R15 containing 100 U/ml recombinant human IL-2 (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IL-10 clone 948505 (MAB9184) and the isotype controls mouse IgG2a (MAB003) and mouse IgG1 (MAB002) (all from R&D systems).
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bioRxiv - Immunology 2021Quote: ... We used 3 different kits to quantify sCD25 by Human CD25/IL-2R alpha Quantikine ELISA Kit (R&D System, Cat# DR2A00), IP-10 by Human IP- 10 ELISA Kit (CXCL10 ...
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bioRxiv - Immunology 2020Quote: ... non-tissue culture plates were coated overnight at 4゜C with recombinant human B7-H3 protein (R&D Systems, Minneapolis, MN) at 10 μg/well in 500 μL of PBS ...
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bioRxiv - Immunology 2020Quote: ... were blocked using 5μg of mouse serum IgG for 10 min at RT then incubated with mouse anti-human ACE2 (R&D systems, FAB9333G), mouse antihuman CD81 (BD Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... The surface expression of hACE2 was confirmed by flow cytometry using anti-human ACE2 AlexaFluor 647-conjugated Ab (R&D Systems, # FAB9332R). The expression was further confirmed by immunoblot using hACE2 Ab (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2020Quote: ... The amount of BMP2 bound to the surfaces was quantified by indirect enzyme-linked immunosorbent assay (ELISA) using DuoSet human BMP2 kits’ (R&D Systems). After the samples were coated with BMP2 ...
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bioRxiv - Immunology 2022Quote: ... APRIL and CD83 levels were measured in the serum/plasma of participants of the four study groups by using the Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit (R&D systems), APRIL Human ELISA Kit (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... + 10% heat-inactivated fetal bovine serum (HyClone) (hereafter referred to as “infection medium”) containing 10 nM human IL-8 (R&D Systems) and left to adhere to tissue culture-treated plastic coverslips (Sarstedt ...
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bioRxiv - Immunology 2022Quote: Investigations into the effect of SmCI-1 and MutSmCI-1 on cytokine production were examined using a Human XL Cytokine Luminex Performance Panel Premixed Kit (bio-techne/R&D systems). Human blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2022Quote: ... Monocyte differentiation into macrophages was performed for 5-7 days in the presence of human M-CSF (100 ng/ml) (R&D system). On the day of the assay ...
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bioRxiv - Bioengineering 2022Quote: ... volume needed.25 Multiplexed Luminex assays were performed to measure cytokines in 48- or 72-hour undiluted conditioned medium throughout the co-culture period using the magnetic Human Luminex XL Cytokine 45- PLEX pre-mixed kit (R&D systems). Protocols provided by the manufacturer were adapted to allow the assay to be performed in a 384-well plate to avoid introducing batch variability ...
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bioRxiv - Cancer Biology 2022Quote: Human cytokine secretion was assessed in mouse plasma samples using the Human XL Cytokine Luminex Performance Panel Premixed Kit (#FCSTM18, R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-ALIX and rabbit anti-CHMP4B antibodies were described previously (Christ et al, 2016). Human Galectin-3 Alexa Fluor 488-conjugated antibody (cat. no. IC1154G) was purchased from R&D Systems. Mouse anti-LAMP1 (cat ...
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bioRxiv - Cell Biology 2022Quote: ... Total RANKL was calculated by measuring free RANKL and in complex with OPG using an in house developed OPG/RANKL complex sandwich ELISA by using a human capture RANKL antibody (part of cat#DY626, R&D Systems) and a human detection OPG antibody (part of cat#DY805 ...
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bioRxiv - Cell Biology 2022Quote: ... Both mouse and mixed organoids were exposed to recombinant human IL-11 (rhIL-11, R&D systems, Minneapolis, MN, USA, #218-IL) during the entire organoid culture period of 14 days.
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bioRxiv - Cancer Biology 2022Quote: ... The samples were further processed following the Human Phospho-Receptor Tyrosine Kinase Kit (R&D Systems Europe, Ltd., Abingdon OX14 3NB, UK) manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 1×106 PMBCs were incubated with 20 ng of MIP-1β (recombinant human CCL4L1/MIP-1β isoform LAG-1; R&D Systems) for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... to detect human IL-8 was done using culture supernatants at 1:100 dilution in a Quantikine ELISA kit (R&D Systems) per manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and stained for total and relevant phosphoproteins with the following antibodies in one of the three tubes: Tube 1: mouse monoclonal anti-human RPS6 (Clone 522731, catalog # MAB5436, R&D Systems) and rabbit polyclonal anti-human Phospho-RPS6 (Catalog # AF3918 ...
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bioRxiv - Cell Biology 2022Quote: The cDNA encoding the extracellular N-terminal IgV domain of human CD47 (residues 19-141) was amplified from the plasmid hCD47 VersaClone cDNA (RDC1523, R&D systems) and was cloned in the expression vector pGEX-4T-1 (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... Cytokine profiling was carried out following the manufactures instructions using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems; ARY022B). The kit contained membranes with dots of 105 different immobilised cytokine antibodies dotted with two technical replicates each (Supplementary Figure 5) ...
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bioRxiv - Pathology 2024Quote: ... Cytokines released from hiPSC-CMs into the supernatants were analyzed using Proteome Profiler Human XL Cytokine Array Kit (Cat. No. ARY022B R&D Systems). Chemiluminescence from the membrane was detected and scanned on an iBright Imaging System FL1000 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... we used both mouse (111 analytes) and human (105 analytes) proteome profiler XL kits (R&D Systems, Cat No. ARY028 and ARY022B). We screened the cell culture supernatant from Day 7 spheroid cultures with and without SIS ECM ...
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bioRxiv - Molecular Biology 2024Quote: ... Resting or ADP (10 μM)-stimulated platelets (4 × 103/μL) were pretreated with the inhibitor hDcR3 (10 μg/mL, recombinant human decoy receptor 3 protein, R&D Systems) for 10 min and then allowed to adhere to the coated coverslips for 60 min at RT under normoxic or hypoxic conditions ...
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bioRxiv - Neuroscience 2024Quote: The levels of secreted BDNF in cell culture supernates were measured using enzyme-linked immunosorbent assay (ELISA). ELISA kit for human BDNF (Cat. DBD00) was sourced from R&D Systems, and the experiments were conducted according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.