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Citations for R&D Systems :
2101 - 2150 of 2461 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Molecular Biology 2019Quote: ... administered intravenously to BALB/C mice at a dose of (0.05mg/kg of body weight and measured for serum hEpo concentration using Human Erythropoietin Quantikine IVD ELISA kit (R&D Systems cat. no. DEP00) at 6 hours post injection.
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bioRxiv - Pathology 2020Quote: ... The culture media was then harvested and the concentrations of human IL-1β and IL10 were measured using ELISAs (DY201 and DY217B, R&D Systems, Oakville, ON).
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bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
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bioRxiv - Physiology 2021Quote: ... HOMA-IR was calculated as (fasting glucose × fasting insulin)/22.5 and HOMA-B as (20 × fasting insulin)/(fasting glucose – 3.5).(31) GDF15 concentration was measured with Human GDF-15 Quantikine Elisa Kit (R&D Systems, Minneapolis, MN, USA). Serum GDF15 samples during and after pregnancy were diluted 1:64 and 1:4 ...
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bioRxiv - Immunology 2021Quote: Concentration of Activin A in human and mouse serum samples were performed using sandwich ELISA-Activin A immunoassay kit from R&D system (Cat DAC008).
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bioRxiv - Immunology 2021Quote: ... Immunostaining nanoparticle tracking analysis (NTA) was performed using fluorescently labeled antibody conjugate directed against human CD63 (AlexaFluor488-conjugated clone 460305; R&D Systems (Minneapolis, USA)) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Genetics 2020Quote: ... culture medium was replaced with KSR medium (Chambers et al., 2009, 2011) supplemented with 200 ng/ml of recombinant human Noggin (R&D Systems, Minneapolis, USA) and 10 μM of SB431542 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... from both tracheobronchial and alveolar infected ALI tissues and used to measure TH1/TH2 responses and growth factors with a customized 22-multiplex panel Human Magnetic bead Luminex assay (R&D Systems, MN, USA), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then seeded on the coated flasks and supplemented with 100 ng/ml of human PDGF-BB re-suspended in 0.1%BSA/4mM HCl/PBS (R&D Systems; 200-BB-050) daily for at least 7 days ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and Connective Tissue Growth Factor (CTGF) (DY9190-05) were measured using Human DuoSet Enzyme linked immunosorbent assay (ELISA) kits (R&D Systems, Abingdon, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Bioengineering 2024Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate were cultured in DMEM base medium supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1% v/v FBS with or without 100 ng/ml Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN). Medium change was performed at day 3 and images were taken at 3 and 5 days using a Keyence BZ-X800 Fluorescence Microscope (Keyence ...
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Bioengineering 2024Quote: Dye-labeled antimicrobial therapeutics (VHHα-(ABD)2-(EEG)6-Sx-POL-Cy7 or VHH16-ABD-(EEG)6-S17-PNT4-sulfo-Cy7) were incubated with recombinant human ADAM10 (R&D Systems, MN, U.S.A.) at 10 μM and 250 nM final concentrations respectively ...
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bioRxiv - Bioengineering 2024Quote: The effective concentration of IL-15 in the samples was assessed using the Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA). All subsequent steps after sample collections were carried out following the instructions provided by the kit ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
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bioRxiv - Bioengineering 2023Quote: ... proteins were extracted via lysis using a lysis buffer from the Proteome Profiler™ Human Apoptosis Array Kit (ARY009, R&D Systems, Minneapolis, MN) kit ...
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bioRxiv - Microbiology 2023Quote: The levels of CX3CL1 were determined according to manufacturer’s instructions using the Human CX3CL1/Fractalkine DuoSet ELISA and DuoSet ELISA Ancillary Reagent Kit (R&D Systems, Minneapolis, Minnesota, USA). Supernatants were diluted 1:1 with Reagent Diluent for all measurements and performed in duplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... chemokines and acute phase proteins were simultaneously assessed using the proteome profiler human XL cytokine array kit [ARY005B] from R&D Systems (Abingdon, UK). Cell lysates were obtained from A375 following treatment with 0.1 MOI of RT3D and 0.1 mM talazoparib at 48 hours post-treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Cancer Biology 2024Quote: Human CCL2/MCP-1 and M-CSF/CSF1 were measured by ELISA using DuoSet kits (DY279, DY216, respectively) from R&D Systems (Minneapolis, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2024Quote: Protein profiling of the secreted factors from each cell culture was performed using the Proteome Profiler Human XL Cytokine array kit (R&D systems, Minneapolis, MN) according to the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The iPSCs were functionally characterized by checking for their ability to differentiate into the three germ layers (Human pluripotent stem cell functional identification kit, R&D systems, Cat#SC027B) according to the manufactures protocol ...
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bioRxiv - Immunology 2024Quote: ... 100 µl of each serum diluted were added to wells of polystyrene plates coated with human IFN-α2a and IFN-β antigens (R&D Systems Inc). All serum samples were run twice in duplicate ...
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bioRxiv - Genetics 2021Quote: ... and 2 μg/ml of mouse anti-ACE2 (R&D Systems;; Catalogue: MAB933;; RRID: AB_2223153). Secondary antibodies used were ...
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bioRxiv - Immunology 2021Quote: ... plates were blocked in 300 ml of 1x reagent diluent concentrate 2 (R&D Systems) for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... Replacement of CHIR99021 by 20 ng/mL FGF-2 (223-FB-500, R&D Systems), followed by maintenance in 15 ng/mL VEGF and 5 ng/mL FGF-2 induced differentiation into the hematopoietic lineage according to a modified protocol from Grigoriadis et al ...
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bioRxiv - Immunology 2021Quote: ... cells have been expanded using 20ng/ml mouse IL-2 (R&D System 402-ML). Cells were considered into exhaustion-like state after 4 stimulations (Tex ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with growth factors at the following concentrations: LM-22A4 (2 μM, R&D Systems), GDNF (2 ng/mL ...
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bioRxiv - Systems Biology 2020Quote: ... supplemented with growth factors at the following concentrations: LM-22A4 (2 µM, R&D Systems), GDNF (2 ng/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were diluted at 2 μg/ml using Calibrator Diluent RD5-18 (R&D Systems). Hyaluronan quantification was then performed using the Hyaluronan Quantikine™ ELISA kit from R&D Systems (DHYAL0).
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bioRxiv - Cancer Biology 2022Quote: ... using additional reagents from the DuoSet ELISA ancillary reagent kit 2 (R&D Systems DY008). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... PlGF-2 (3 – 30 ng; 5 µl; cat.465-PL/CF, R&D System, USA), VEGF-E (3 – 30 ng ...
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bioRxiv - Bioengineering 2021Quote: ... Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000, R&D Systems, Cat#MAB10474) was used for the detection of SARS-CoV-2 ...
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bioRxiv - Cancer Biology 2019Quote: ... the secretion levels of Matrix metallopeptidase (MMP)-2 and -9 (R&D system, MN, USA) and transforming growth factor (TGF)-β1 (R&D system ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: ... cells were stimulated with increasing amounts of IFNs (α2 (R&D Systems, Cat#11101-2), β (R&D Systems ...