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Citations for R&D Systems :
2151 - 2200 of 2461 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: IL-4 complexes were generated by incubating 2 μg mouse rIL-4 (R&D Systems) with 10 μg LEAF purified anti-mouse IL4 antibody (clone 11B11 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody was added for ULBP 1 and 2/5/6 only (R&D Systems). Cells were then washed and fixed in 4% paraformaldehyde ...
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bioRxiv - Cancer Biology 2024Quote: ... at a 1:10 ratio and plated on 2-well comet slides (R&D Systems). Cells were then lysed overnight and immersed in alkaline unwinding solution as per manufacturer’s protocol (Trevigen) ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 2 days with 200 ng/mL FGF-9 (R&D Systems, 273-F9) and 1 µg/mL Heparin (Millipore Sigma ...
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bioRxiv - Genetics 2024Quote: ... as per the manufacturer’s instructions (Mouse TGF-beta 2 Quantikine ELISA Kit, R&D Systems). The samples were diluted (1:80) ...
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bioRxiv - Cell Biology 2020Quote: ... dPSCs were differentiated into each of the three germ layers according to the Human Pluripotent Stem Cell Functional Identification Kit (R&D Systems®, Catalog # SC027B).
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... FFPE pancreas sections were incubated overnight at +4°C with primary antibody monoclonal mouse anti-human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were subsequently washed with PBS without Ca2+ and Mg2+ and then incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... ELISAs were performed according to the manufacturer’s instructions for IL-10 (Quantikine®HS ELISA Human IL-10 Immunoassay, R&D Systems, Inc., Cat# HS100B), sCD14 (Quantikine® ELISA Human sCD14 Immunoassay ...
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bioRxiv - Cancer Biology 2019Quote: ... For both assays the cells were cultured for 72 hrs at 37 °C and 5% CO2 and Cell free supernatants were then collected and used to perform IFN-γ ELISA using the Human IFN-γ DuoSet assay (R&D system, DY285).
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bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
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bioRxiv - Microbiology 2020Quote: ... cell-free supernatants were collected and used to evaluate the concentrations of TGF-β1 with human and rat TGF-β1 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Physiology 2020Quote: ... The measurement was performed by Enzyme-Linked Immunosorbent Assay (ELISA) using the Human PCSK9 Quantikine ELISA kit (R&D Systems, Catalog no. DPC900, Minneapolis, USA) according to kit instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
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bioRxiv - Cell Biology 2022Quote: The relative levels of angiogenesis-related cytokines in the MSC-CM were analysed using the Proteome Profiler Human Cytokine Array Kit from R&D systems (Abingdon, UK, ARY022B) per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse tibialis anterior muscles were injected with 50 uL solution of 50,000 human FAPs resuspended in 50% glycerol in PBS with either 29ng/ul concentration of recombinant DLK1 resuspended in PBS (R&D Systems, #1144-PR-025) or with control (identical volume of PBS ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.
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bioRxiv - Cancer Biology 2024Quote: ... lysates of specific conditions were collected and the relative phosphorylation status of 37 kinase phosphorylation sites was evaluated by using a Human Phospho-kinase Array kit (ARY003C, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Immunology 2023Quote: Cells were stimulated for 18-24h in complete medium at 37˚C with addition of 50- 100U/ml human IFNγ (R&D Systems #285-IF-100).
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fibroblasts at 70-80 % confluent were serum starved overnight and treated with 50ng/ml of recombinant human PDGF-DD (R&D system, 1159-SB-025) for 24h.
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bioRxiv - Microbiology 2023Quote: ... The concentration of IL-8 released in basolateral culture media were assayed by ELISA using the human ELISA development kits (DuoSet DY208, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: MCF7 and T47D cell lines were perturbed using 5 ng/mL of recombinant human TGF-β (R&D Systems 240-B-002/CF, Minneapolis, MN) over the course of three days ...
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bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
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bioRxiv - Cancer Biology 2024Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Cancer Biology 2024Quote: ... To this goal we used a specific spectrofluorimetric activity assay (Human Active Caspase-3 Immunoassay Quantikine ELISA, Catalog Number KM300, R&D Systems, Inc., Minneapolis, MN). In summary ...
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bioRxiv - Immunology 2024Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and TNF-α-treated CM with FGF-2 neutralizing antibody (NAb; AF-233, R&D Systems). On day 7 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were immuno-precipitated with 2-5 µg of either anti-GFI1 (AF3540, R&D systems), anti-H3K4me1 (ab8895 ...
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bioRxiv - Neuroscience 2019Quote: ... and BSA (2%) and stained with following primary antibodies: PI16 (1:75, R&D Systems AF4929), GLUT1 (1:250 ...
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bioRxiv - Bioengineering 2020Quote: ... Mouse IL-28 A/B (IFN-lambda 2/3) Du-oSet ELISA (DY1789B, R&D Systems), was used to assess cross-reactivity.
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-MAP2 (aka Microtubule-Associated Protein 2; 1:250; R&D Systems, Cat #: MAB8304, RRID:AB_2814693), rabbit anti-Myosin7 (MYO7 ...
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bioRxiv - Immunology 2020Quote: ... then washed and stimulated in the same media with 5ng/mL hIL-2 (R&D Systems), 12ng/mL hIL-10 (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... in defined media supplemented with 2% B27 and 20ng/ml EGF and bFGF (R&D System). Neurospheres were passaged once and conditioned medium was recovered before the second passage ...