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Citations for R&D Systems :
1851 - 1900 of 2461 citations for Siglec 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Cytokine secretion from primary CF-HBECs was measured with the Human IL-8/CXCL8 DuoSet ELISA (#DY208, R&D Systems, Minneapolis, MN). Several samples were also screened with MILLIPLEX MAP Human Cytokine/Chemokine 41-Plex cytokine assay (Millipore) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-1β and TNFα and IL-6 in cell culture supernatants or serum were measured using Human IL-1 beta/IL-1F2 DuoSet ELISA (DY201, R&D Systems), Mouse IL-1 beta/IL-1F2 DuoSet ELISA (DY401 ...
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bioRxiv - Immunology 2021Quote: ... The immunoblots were probed overnight at 4 °C or 1 hour at room temperature with anti-human IL-1β antibody (AF-201-NA, R&D Systems); anti-human Caspase-1 antibody (06-503 ...
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bioRxiv - Genetics 2021Quote: ... Cell culture supernatants were collected and assayed for the secretion of pro-Col1A1 using Human Pro-Collagen I alpha 1 DuoSet ELISA kit (DY6220-05, R&D Systems) as described by the manufacturer.
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bioRxiv - Immunology 2020Quote: ... Human serum GzmA levels from healthy donors and patients with confirmed abdominal sepsis at diagnose and during the first 72h was monitored by a commercial ELISA kit (Human Granzyme A DuoSet ELISA, R&D Systems) following the manufacturer recommendations.
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bioRxiv - Developmental Biology 2020Quote: ... An NRG1-β1 ELISA assay was performed according to the protocol of the manufacturer (Human NRG1-β1 DuoSet ELISA, R&D systems). Absolute NRG1-β1 concentration was calculated based on the standard curve.
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bioRxiv - Immunology 2022Quote: ... monocytes were cultivated for 6 days with a change of medium at day 3 in RPMI 1640/10% human serum and 10 ng/ml recombinant M-CSF (R&D Systems). At day 6 the medium was replaced with RPMI 1640/10% human serum and used for experiments on day 7.
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bioRxiv - Genomics 2019Quote: ... Serum IL-6 levels were measured using the Quantikine® Enzyme Linked Immuno-Sorbent Assay Human IL-6 Kit (R&D Systems) following the manufacturers standard protocol ...
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bioRxiv - Immunology 2019Quote: ... and resuspended for culture at 37°C + 5% CO2 for 1 h prior to stimulation with recombinant human TNFα (R&D Systems). Following stimulation ...
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bioRxiv - Physiology 2020Quote: ... postnatal C57BL/6J mice at age of day 20 were intraperitoneally (I.P.) injected with recombinant human NTF3 (50μg/kg body weight, R&D Systems, Minneapolis, MN) daily for 10 days ...
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bioRxiv - Pathology 2019Quote: Supernatants were tested for 6 analytes (TNFa, IL-6, IL-8, IL-10, IL-1b, IL-1a) on a custom Human magnetic Luminex plate (R&D systems LXSAHM ...
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bioRxiv - Microbiology 2021Quote: The relative levels of selected human cytokines and chemokines was determined in parallel using a membrane-based antibody array (ARY005B, R&D Systems). Cells were seeded at 7.5 × 105 cells per well in a tissue-culture treated six-well plate ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated for 16 h prior to infection with addition of 50 IU mL-1 human IFNγ (285-IF, R&D Systems). Induction of GBP expression in the Dox-inducible cells was performed with 200 ng mL-1 Dox overnight (D9891 ...
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bioRxiv - Bioengineering 2020Quote: Lambda interferon levels were evaluated by human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA (DY1598B, R&D Systems, USA). Biological activity experiments were performed using ...
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bioRxiv - Neuroscience 2021Quote: ... Protein expression for the expressed human LTα transgene was measured in snap-frozen rat CSF using a human TNF-beta/TNFSF1 Quantikine ELISA kit (DTB00, R&D systems) as per manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Hippocampal homogenate samples were used to determine NRG1 levels with the Human NRG1-β1 ELISA (DY377, R&D Systems, Minneapolis MN, USA). Samples were diluted in kit assay buffer (1:6 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... counted and 5 μL/106 cells of Human EGFR (Research Grade Cetuximab Biosimilar) Alexa Fluor® 488-conjugated Antibody (R&D Systems) was added ...
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bioRxiv - Immunology 2021Quote: ... Cell supernatant was collected and 1 ml was used for Proteome Profiler™ Human Cytokine Array (ARY005B, R&D Systems, Inc., USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: GFP-binding protein (GBP) (Rothbauer et al., 2008) was fused to the Fc domain of human IgG (pIg-Tail) (R&D Systems), tagged with His6 in pET28a (EMD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: Phosphorylation of several kinases and expression levels of matrix metallopeptidases (MMPs) were analyzed using Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) and Human MMP Antibody Array Kit (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... cells were wash three times with PBS to remove any residual FBS and followed by 1-hour treatment with goat anti-human ACE2 antibody (R&D Systems) or anti-hDDP4 antibody (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: Monocyte-derived macrophages (MDMs) were obtained by stimulation of PBMC cultures with 15 ng/mL recombinant human M-CSF (R&D systems) and 10% human AB serum (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: Cells were stimulated for 16 h prior to infection in complete medium at 37°C with addition of 50 IU/mL human IFNγ (285-IF, R&D Systems). Induction of Doxycycline-inducible cells was performed with 200 ng/mL Doxycycline overnight (D9891 ...
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bioRxiv - Systems Biology 2021Quote: ... washed once with FACS buffer (1%BSA in PBS and 5mM EDTA) and incubated for 40 minutes in FACS buffer containing 0.75ug of human ACE2-488 conjugated antibody (R&D Systems FAB9332) and 1:1000 7-AAD (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The secretome for each specimen was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (Ary007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Bound NAMPT molecules were detected using a biotinylated antibody for NAMPT and HRP-streptavidin (Human PBEF/Visfatin DuoSet ELISA, R&D Systems). Signals obtained on BSA-coated wells were used to remove non-specific binding for each NAMPT concentrations to obtain specific binding values ...
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bioRxiv - Bioengineering 2020Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (ARY009, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: The relative expression levels of cellular stress related proteins of iCMs (n=3) were analyzed using the Proteome Profiler Human Cell Stress Array (ARY018, R&D Systems), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... conditioned medium was collected and the levels of secreted human CXCL8 and murine CXCL1 were detected with DuoSet ELISA (R&D systems) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and human IL-13 (monoclonal capture antibody MAB213 and polyclonal detection antibody BAF213) were purchased from R&D Systems (Minneapolis, MN, USA).
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Immunology 2022Quote: ... Calprotectin levels were determined in optimally diluted (1:20) extracts using Human-S100A8/S100A9 Heterodimer DuoSet-ELISA kit from R&D Systems. Zonulin levels were determined in similarly diluted extracts using human Zonulin Stool ELISA kit from ALPCO ...
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bioRxiv - Molecular Biology 2022Quote: ... The levels of IL11 in equal volumes of cell culture media were quantified using Human IL11 Quantikine ELISA kit (D1100; R&D Systems). Both assays were performed according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... monolayers grown with the differentiation media for 7 days were stimulated with human IFNβ (100 or 300 IU/ml, R&D systems) or IFNλ2 (10 or 30 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: Cytokines were measured in the supernatants from ASC cultures and ASC-enriched patches using the human cytokine base kit A (R&D Systems) combined with a magnetic Luminex assay (Bio-plex 200 ...
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bioRxiv - Immunology 2020Quote: ... the cells were cultured for 5 days in RP10 in the absence or presence of 10 ng/ml human IL-7 (R&D System). Cells were then stimulated for 6 hours with 0.5 μg/ml anti-CD49d clone L25 and 0.5 μg/ml anti-CD28 clone L293 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies were immobilized on CM5-Sepharose chips coated by standard amine coupling chemistry to a density of about 600 response units (RU) Measurements were carried out at 25°C by injecting 250 μl of recombinant human TNFa (R&D Systems) at concentrations from 0.2 to 50 nM with a flow rate of 35μl/min ...
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bioRxiv - Immunology 2020Quote: Levels of human IL-1β (DY201) and IL-18 (DY318) were measured in the cell supernatants using ELISA kits from R&D Systems. Human IL-6 (# 88-7066-86 ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated at 37 °C for 30 minutes with 15 μm Forskolin and 1:3 serial dilutions of potential ligands with the following starting concentrations: 10 μM recombinant human CXCL17 (R&D Systems), 10 μM lysophosphatidic acid (LPA ...
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bioRxiv - Microbiology 2021Quote: ... Barrier integrity was further assessed using the Human E-cadherin Quantikine enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN). Absorbance was measured at 450 nm using a BioTek Synergy H2 plate reader and concentrations of E-cadherin were determined against a standard curve according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the following Abs were used: polyclonal sheep anti-human CD47 (AF4670) and sheep IgG HRP-conjugated Ab (HAF016) from R&D system; mouse anti-HA mAb (16B12) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fibroblasts+rhTGFβ1 medium was incubated with 1 μg/mL of anti-human HGF antibody or with the respective mouse IgG1 isotype control (both from R&D Systems) for 1 hour at 37ºC.
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bioRxiv - Cell Biology 2022Quote: The concentration of active TGF-β1 was measured using the Human /Mouse /Rat /Porcine /Canine TGF-beta 1 Quantikine ELISA kit (DB100B, R&D Systems). Briefly ...
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bioRxiv - Immunology 2022Quote: The supernatants collected from LPS-stimulated moDCs with or without fhFABP1 (25 μg/ml) were pooled from 4 donors in equal volume and subjected to the Proteome Profiler Human XL Cytokine Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein) commercially available from R&D Systems by adaptation of a published protocol (Bally et al. ...
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bioRxiv - Cancer Biology 2022Quote: Changes in kinase phosphorylation upon exposure to the SASP was assayed using the Proteome Profiler Human Phospho-Kinase Array (R&D Systems). Briefly ...
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bioRxiv - Systems Biology 2019Quote: ... five µg of glycopeptide in 50 mM Tris-HCl pH 7.4 was mixed with one µg recombinant human C1GalT1/C1GalT1C1 protein (R&D Systems, NM) in the presence of either 0.5 mM UDP-Gal (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems). After 3 days of ActA treatment EBs were plated on fibronectin (20 μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... The supernatants from the serovar D infections were subjected to multiplex ELISA analyses in triplicate setting using a custom designed 27-plex human magnetic Luminex assay #LXSAHM (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total CREB and pCREB were measured using the Human/Mouse/Rat Phospho-CREB (S133) Cell-based ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...