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Citations for R&D Systems :
1901 - 1950 of 2036 citations for GITR Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 5 mM beta-glycerophosphate and 1% insulin-transferrin-selenous acid (osteogenic media) and stimulated them the next day with recombinant human BMP2 (300 ng/ml) (R&D Systems, MN) in serum-free osteogenic media ...
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bioRxiv - Systems Biology 2023Quote: Where indicated the cells were stimulated with the final concentration of 10 ng/ml of recombinant human TNF-α protein (R&D Systems) or 100 ng/ml of recombinant human interleukin 1 beta (IL-1β ...
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bioRxiv - Biochemistry 2023Quote: ... and 250 μg of protein from each condition was incubated with a membrane array from the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). Membranes were processed according to the manufacturer’s instructions and imaged using an ImageQuant LAS 4000 (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: Levels of human IL-18 and mouse IL-1β were measured in the cell supernatants using ELISA kits from R&D Systems (DY318) and (DY401-05) ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of isolated EV sample was mixed with 4 μL of Alexa488 anti-human TNFRSF10B antibody (FAB6311G, R&D Systems, Minneapolis, MN) and 2 μL of PE anti-human CD63 antibody (561925 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were used to quantify the amount of cyto/chemokines produced by the urothelial organoids before and after infection using a human premixed multi-analyte customized kit in a Luminex® bead based immunoassay (R&D systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: The receptor tyrosine kinase (RTK) activities in cells after drug treatment were analyzed by a Proteome Profiler Human Phospho-RTK Array Kit (R&D Systems, ARY001B) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 3 independent vials of iCell microglia were seeded at 5 x 104 cells per well across 3 independent 96-well plates and treated the following day with vehicle or 5 ng/mL human IFN-β (R&D Systems). Cells were treated for 6 or 24 h prior to collection and stored at −80°C prior to RNA extraction.
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bioRxiv - Bioengineering 2023Quote: ... The relative expression levels of apoptosis related proteins of post I/RI iCMs (n=3) were analyzed using the Proteome Profiler Human Apoptosis Array (R&D Systems, #ARY009), according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... were assessed from the culture supernatant of the activated CD4+ T cells by Human Th9/Th17/Th22 Luminex Performance Assay 17-plex Fixed Panel (R&D systems, USA). The assay was performed according to manufacturers instructions and samples diluted 1:2 with calibrator diluent ...
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bioRxiv - Immunology 2023Quote: ... in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA; R&D Systems) and/or 50 ng/ml IFN-γ (285-IF ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were incubated with PBSTBA diluted 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) for 2 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... for 10 minutes on ice and incubated with fluorescence-conjugated antibodies for 15 minutes on ice: PB2 PE (phycoerythrin) (human, R&D Systems #FAB53291P), PB2 APC (human ...
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bioRxiv - Physiology 2023Quote: ... ciCMVEC were placed in serum-free media 1h before treatment with 10 ng/ml of Recombinant Human TNF-α Protein (210-TA-005; R&D systems) or vehicle (PBS ...
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bioRxiv - Immunology 2023Quote: ITCH ubiquitination reactions were carried out using the manufacturer’s protocol for the Human ITCH/AIP4 Ubiquitin Ligase Kit (R&D Systems, K-270). 10X reaction buffer ...
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bioRxiv - Bioengineering 2024Quote: ... IDUA activity was assessed using 4-methylumberlliferyl-alpha-L-iduronide (Glycosynth, Cheshire, England) and recombinant human alpha-L-iduronidase protein (R&D systems, Minneapolis) following manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with an equal volume of 1 mg/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2h ...
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bioRxiv - Immunology 2024Quote: ... 1:100 diluted human plasmas and a dilution series of the indicated neutralizing anti-IFN antibodies (IFNβ, PBL assay science, 31410-1; IFNλ1: R&D systems, MAB15981 ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were seeded in tissue culture-treated 12-well plates coated with human fibronectin (5 µg/cm2) (R&D Systems, Cat. # 1918-FN). Stimulated and unstimulated groups were formed as described above ...
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bioRxiv - Bioengineering 2024Quote: ... one T25 tissue culture flask was given media spiked with 1 ng/mL recombinant human TNF-α (R&D Systems, Cat. # 210-TA) (stimulated group ...
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bioRxiv - Neuroscience 2024Quote: Secreted proteins in iTF-Microglia conditioned media were measured using the Proteome Profiler Human Cytokine Array Kit (R&D Systems; Cat. No. ARY005B) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... for 24 hours and rested overnight in C-RPMI with 100µM ferric ammonium citrate (FeAC) and 200U/ml of human recombinant IFN-γ (R&D Systems, MN USA) unless otherwise stated.
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bioRxiv - Developmental Biology 2020Quote: Embryonic immortalised mouse epicardium-derived cells (mEPDC) were treated with 2.0 µg/ml human recombinant transforming growth factor (TGF)-β (R&D Systems; 240-B) for 30 hrs to induce EMT ...
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bioRxiv - Developmental Biology 2019Quote: ... Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction.
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bioRxiv - Cell Biology 2019Quote: ... and expanded chondroprogenitor cells were re-suspended at 2.5 × 105 cells / mL in chondrogenic medium supplemented with 10 ng/ml human transforming growth factor beta 3 (TGF-β3; R&D Systems, Minneapolis, MN). Chondrogenic pellets were cultured at 37°C for 28 days ...
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bioRxiv - Neuroscience 2020Quote: ... or the newly designed FRET Aβ34-site peptide (10 µM) were digested at 37° C by human BACE2 (R&D systems, 1 ng/µL) in presence or absence of the stated inhibitors for 2h ...
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bioRxiv - Bioengineering 2021Quote: Conditioned media from each cell-line/passage (at the time of cell harvest) were collected and prepared for cytokine profiling using the Human Premixed Multi-Analyte Magnetic Luminex Assay (R&D Systems, Minnneapolis MN) (Supplemental Table 3) ...
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bioRxiv - Biochemistry 2021Quote: Interleukin (IL)-6 in the patient plasma samples was measured by the Quantikine ELISA kit for human IL-6 (R&D Systems, Minneapolis, MN), as previously [29] ...
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bioRxiv - Microbiology 2020Quote: ... Ad-transduced cells were incubated for 24h before performing flow cytometry staining for surface expression of hACE2 using goat anti-human mAb AF933 (R&D Systems, Minneapolis, MN) used at a final concentration of 10μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Human ACE2 Biotinylated Antibody (Cat # BAF933) and Recombinant Spike S1 RBD protein (Cat # NBP2-90982) were obtained from R&D Systems (Minneapolis, MN, 55413) and NOVUS Biologicals (Centennial ...
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bioRxiv - Molecular Biology 2020Quote: Apical washes and basolateral media samples were clarified by centrifugation and analyzed using the Magnetic Luminex® Performance Assay Human High Sensitivity Cytokine Base Kit A (R&D Systems, Inc). Apical washes were diluted 1:2 ...
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bioRxiv - Cell Biology 2020Quote: Conditioned media of papillary and reticular fibroblasts from the three donors were analysed using human angiogenesis antibody array (R&D systems, Minneapolis MN, USA). Images were acquired using Fusion Capt Advance FX7 (Vilber ...
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bioRxiv - Immunology 2022Quote: Cells diluted to 1 × 106 per microtiter plate well were incubated for 45 min at room temperature with a 1 in 100 dilution of a mouse IgG2a antibody to human/hamster ACE-2 (clone 171606 R&D Systems, Minneapolis, MN). After washing ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were aspirated and incubated overnight at 4°C with either: primary goat polyclonal antibody to Human ACE-2 (AF933; R&D Systems; 1:100); primary rabbit monoclonal antibody to TMPRSS2 (ab92323 ...
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bioRxiv - Microbiology 2021Quote: ... were cultured for 7 days in RPMI supplemented with 5% decomplemented autologous human blood plasma and 10 ng/mL M-CSF (R&D system, #216-MC) to obtain MDMs ...
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bioRxiv - Bioengineering 2020Quote: ... Microvascular fragment-containing gels were cultured in serum-free media supplemented with 10 ng/mL recombinant human vascular endothelial growth factor (rhVEGF; R&D Systems, Minneapolis, MN) (55) ...
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bioRxiv - Molecular Biology 2019Quote: ... administered intravenously to BALB/C mice at a dose of (0.05mg/kg of body weight and measured for serum hEpo concentration using Human Erythropoietin Quantikine IVD ELISA kit (R&D Systems cat. no. DEP00) at 6 hours post injection.
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bioRxiv - Pathology 2020Quote: ... The culture media was then harvested and the concentrations of human IL-1β and IL10 were measured using ELISAs (DY201 and DY217B, R&D Systems, Oakville, ON).
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bioRxiv - Systems Biology 2021Quote: ... The concentration of CCN4 in the cell culture media from those wells was assayed using the Human WISP-1/CCN4 DuoSet ELISA Kit (R&D Systems, Minneapolis, MN) to confirm CCN4 knockout ...
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bioRxiv - Physiology 2021Quote: ... HOMA-IR was calculated as (fasting glucose × fasting insulin)/22.5 and HOMA-B as (20 × fasting insulin)/(fasting glucose – 3.5).(31) GDF15 concentration was measured with Human GDF-15 Quantikine Elisa Kit (R&D Systems, Minneapolis, MN, USA). Serum GDF15 samples during and after pregnancy were diluted 1:64 and 1:4 ...
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bioRxiv - Immunology 2021Quote: Concentration of Activin A in human and mouse serum samples were performed using sandwich ELISA-Activin A immunoassay kit from R&D system (Cat DAC008).
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bioRxiv - Immunology 2021Quote: ... Immunostaining nanoparticle tracking analysis (NTA) was performed using fluorescently labeled antibody conjugate directed against human CD63 (AlexaFluor488-conjugated clone 460305; R&D Systems (Minneapolis, USA)) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were serum starved overnight and then incubated with 10 ng/ml recombinant human transforming growth factor-beta 1 (TGF-β1) (R&D Systems, Minneapolis, MN) for indicated times.
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bioRxiv - Genetics 2020Quote: ... culture medium was replaced with KSR medium (Chambers et al., 2009, 2011) supplemented with 200 ng/ml of recombinant human Noggin (R&D Systems, Minneapolis, USA) and 10 μM of SB431542 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... from both tracheobronchial and alveolar infected ALI tissues and used to measure TH1/TH2 responses and growth factors with a customized 22-multiplex panel Human Magnetic bead Luminex assay (R&D Systems, MN, USA), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 5 hours in DermaLife Basal Medium followed by stimulation with human recombinant IL-17A (R&D systems, 50 ng/ml) and TNF-α (R&D systems ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then seeded on the coated flasks and supplemented with 100 ng/ml of human PDGF-BB re-suspended in 0.1%BSA/4mM HCl/PBS (R&D Systems; 200-BB-050) daily for at least 7 days ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and Connective Tissue Growth Factor (CTGF) (DY9190-05) were measured using Human DuoSet Enzyme linked immunosorbent assay (ELISA) kits (R&D Systems, Abingdon, UK) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...