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Citations for R&D Systems :
1501 - 1550 of 2036 citations for GITR Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution) using DuoSet ELISA kits (R&D Systems). DuoSet ELISA Ancillary Reagent Kit 2 was used in conjunction with all DuoSet ELISA kits ...
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bioRxiv - Immunology 2019Quote: ... and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems, Ref. HS600C Lot P178994). All these immunoassays were performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... TNF-α was measured using a Quantikine HS ELISA Human TNF-α (R&D Systems, Ref. HSTA00E Lot P174731) and IL-6 were determined by Quantikine HS ELISA Human IL-6 (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... CXCL8 and Osteopontin (SPP1) proteins in conditioned media was performed using a custom human luminex assay (R&D systems), according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2020Quote: Phosphorylation status and total amounts of 43 kinases were measured using Human Phospho-Kinase Array from R&D Systems. 1.5×106 LN229 cells were stimulated with 400 ng/mL of GAS6 for 10 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... with 10% FBS (Wisent) and 0.5 ng/ml recombinant human Macrophage Colony Stimulating factor (M-CSF) (R&D Systems) to allow for differentiation of monocytes into macrophages ...
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bioRxiv - Bioengineering 2020Quote: ... 97 µL of urine was incubated with 1 µL of human cathepsin B (R&D Systems, 953-CY-010) and 1 µL of DTT for 15 minutes to inactivate the urine cysteine protease inhibitor ...
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bioRxiv - Physiology 2021Quote: ... The medium was supplemented with 100 ng/mL recombinant human M-CSF using CellXVivo™ kit (R&D Systems) and differentiated for 5–7 days prior to use.
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bioRxiv - Cancer Biology 2020Quote: ... Then the tissue sections were incubated overnight at 4°C with antibodies against human DDR1 (R&D Systems, AF2396), CXCL5 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were treated with 10 ng ml−1 of recombinant human IFNγ (R&D Systems, 285-IF-100), 10 ng ml−1 of recombinant human TNFα (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... For the IL-8 neutralization experiments: 0.4 μg/ml of human IL-8 monoclonal antibody (R&D Systems, USA) was added to the apical and basolateral compartment of enteroid monolayers and to the PMN suspensions before they were co-cultured ...
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bioRxiv - Immunology 2022Quote: Human IL-8 was measured from cell culture supernatants using commercially available specific paired ELISA kits (R&D Systems). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... IFN-β amounts in the culture supernatant were measured by human IFN-β ELISA kit (R&D Systems, DIFNB0) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... 0.03 to 3 μg/well of the soluble mouse or human HA-tagged recombinant fibulin 7 (R&D Systems) in 2% dry milk/TBS and 2 mM CaCl2 were added to the wells and incubated for 3 hours ...
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bioRxiv - Immunology 2020Quote: ... and Alexa Fluor 594 labelled anti-human DAP10/HCST (R&D Systems, 1:500 v/v in blocking buffer) to stain for the cytoskeleton and DAP10 clusters respectively ...
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bioRxiv - Physiology 2021Quote: ... The membranes were incubated with a sheep polyclonal antibody to human DG (AF6868; R&D Systems, 1:100 dilution) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6 ...
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bioRxiv - Biochemistry 2021Quote: For TCR-mediated adhesion 96-well plates were pre-coated with 0.5μg human ICAM-1/well (R&D systems). Transfected cells were left untreated or stimulated with anti-TCR antibody (OKT3 ...
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bioRxiv - Physiology 2022Quote: ... Aβ ELISA was performed using a human amyloid β (aa1-42) immunoassay Quantikine ELISA kit (R&D Systems, Inc.).
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bioRxiv - Immunology 2022Quote: ... T cells then were washed and further incubated with recombinant human IL-2 (10 ng/ml; R&D systems) for up to 2 weeks at 37°C incubator with medium change every 3 days ...
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bioRxiv - Molecular Biology 2022Quote: ... human recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF, 50 ng/mL R&D Systems, 7954-GM-020/CF), and interleukin-4 (IL-4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of GDF15 was measured using an ELISA assay for Human GDF-15 DuoSet (R&D systems, # DY957) as per the manufacturer’s instructions on a Tecan INFINITE® 200 Pro spectrophotometer ...
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bioRxiv - Pathology 2020Quote: ... the membranes were incubated with the sheep anti-human collagen I alpha 1 antibody (1:2000, R&D systems) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was titered using a commercial enzyme-linked immunosorbent assay kit (Human IL-1β/IL-1F2 Immunoassay, R&D Systems) following the manufacturer’s instructions and the results were expressed as pg ml−1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells surface ACE2 protein was stained with Alex Flour® 647-conjugated human ACE2 antibody (R&D Systems FAB933R). Live/Dead (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The level of human or mouse CXCL10/IP-10 was determined using a DuoSet ELISA kit (R&D Systems) and the level of mouse IL-6 was determined using a DuoSet ELISA kit (R&D systems ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human galectin-4 (GAL4 P56470) and gelsolin (GSN Q3SX14) from bovine plasma were purchased from R&D Systems and Sigma respectively.
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were exposed to the Human/Mouse Brachyury Affinity Purified Polyclonal Goat IgG antibody (R&D Systems, Catalog # AF2085) overnight at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell-free supernatant was collected and quantified using the Human IL-12p70 DuoSet ELISA (R&D Systems; catalog# DY1270), as per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Osteoclastogenesis was induced by treatment with 25 ng/ml human M-CSF (216-MC, R&D Systems, Abingdon, UK) and 30 ng/ml RANKL (310-01 ...
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bioRxiv - Immunology 2020Quote: ... and 10 ng/mL of human macrophage colony stimulating factor (M-CSF, R&D Systems, catalog number 216-MC) for at least 10 days to generate mature macrophages before use in experiments.
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bioRxiv - Immunology 2021Quote: ... The concentration of sCD25 was measured with the Human CD25/IL-2R alpha Quantikine ELISA kit (R&D Systems) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... secreted matrix metalloproteinases (MMP) concentrations were measured using the magnetic Human MMP Magnetic Luminex Performance Assay (R&D Systems). This assay required dilutions of cell culture supernatants in fresh culture medium at either 2x or 200x to detect the reported analytes within a quantifiable range.
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bioRxiv - Molecular Biology 2022Quote: ... Day 0: 10 ng/ml recombinant human FGF basic/FGF2/bFGF (146 aa) protein (R&D Systems 233-FB), 10 µM RI (what is RI?) ...
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bioRxiv - Bioengineering 2022Quote: ... Enzyme-Linked Immunosorbent Assay (ELISA) kit (DuoSet ELISA Ancillary Reagent Kit and Human Lubricin/PRG4 kit, R&D systems) was used following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cell supernatants were then harvested and assayed for cytokines by Human Cytokine Array (Proteome Profiler, R&D systems, ARY005B), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... 10 ng/mL recombinant human glial cell line-derived neurotrophic factor (GDNF, #212-GD-010, R&D Systems, Germany), 500 µM cAMP (#D0260 ...
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bioRxiv - Physiology 2023Quote: ... Diluted serum was then used to measure human HB-EGF with the DuoSet ELISA Kit (DY259B, R&D Systems).
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bioRxiv - Microbiology 2022Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and analyzed for the presence of secreted factors using Luminex human Discovery Assays (R&D Systems): LXSAHM-02 (MMP-8) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... each well was then filled with 25 μL/well recombinant human TNFα (R&D systems, cat #: 210-TA-100) diluted in cell culture medium (final concentration 10 ng/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The Human Aβ1-42 in soluble and insoluble fractions were qualified with Quantikine ™ ELISA kit (R&D Systems) based on the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... in absence or presence of stimuli (PMA/ionomycin or human recombinant soluble BAFF/BLyS/TNFSF13B Protein (R&D System)) for 18 h at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cytokine array measurements were made using the Proteome Profiler Human XL Cytokine Array Kit (ARY022B) from R&D systems, following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... The differentiated organoids were treated with the indicated doses of recombinant human IL-1β (R&D system, 201-LB) for an additional 48 hrs.
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bioRxiv - Bioengineering 2023Quote: ... and the human BMP-2 DuoSet enzyme-linked immunosorbent assay (ELISA) were purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Biophysics 2023Quote: ... and then a binding buffer solution containing 200 nM of recombinant αVβ5 human integrin extracellular domains (R&D Systems) was slowly added to one of the wells of the fluid chamber ...
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bioRxiv - Microbiology 2023Quote: ... 5 fmol or 10 fmol SpyCEP were incubated with 2 pmol human CXCL8 and CXCL1 respectively (R&D Systems), in a final volume of 100 μl and incubated at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Human vasculature labeling was performed by using a cocktail of goat anti-podocalyxin (1:20, AF1658, R&D Systems), mouse anti-CD31 (1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... and IL6 releases were analysed by Luminex® Assay using the Human Premixed Multi-Analyte Kit (R&D Systems) according to manufacturer instructions and acquiring readings with a BioPlex MagPlex Magneti Beads system (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with mouse anti-human CCR1 antibody (2.5 µg/106 cells; mab145, R&D Systems) for 20 min at RT ...