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Citations for R&D Systems :
1801 - 1850 of 2036 citations for GITR Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The levels of secreted BDNF in cell culture supernates were measured using enzyme-linked immunosorbent assay (ELISA). ELISA kit for human BDNF (Cat. DBD00) was sourced from R&D Systems, and the experiments were conducted according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Following antibodies were used for the labeling of aforementioned transcription factors: Human T-bet/TBX21 Alexa Fluor 488-conjugated Antibody (1:20, IC53851G, R&D Systems), Gata-3 Monoclonal Antibody (TWAJ) ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated affinity purified goat IgG anti-human interferon-γ detection antibody (Cat. #BAF285, lot #ZX2721071, R&D Systems, Minneapolis, Minnesota, United States), recombinant human interferon-γ protein (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... GPC2 CAR expression was determined using APC or PE-conjugated human recombinant GPC2 protein (R&D Systems; 2304-GP-050; 1:500) and CD19 CAR using PE-tagged protein L (58036S ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of TGF-β1 present in the media was quantified using the Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA kit(R&D systems). The results were normalized to the total number of cells in the culture at the moment of media collection.
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bioRxiv - Microbiology 2023Quote: ... Human IL-29/IL-28B (IFN-lambda 1/3) DuoSet ELISA or Human CXCL10/IP-10 DuoSet ELISA reagents (biotechne R&D systems) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... cell-free supernatant was collected and a semiquantitative immunosorbent assessment of cytokine expression was performed using a Proteome Profiler Human Cytokine Array Kit (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 600,000 cells plus culture medium were replaced in a 24 well plate containing a 50-60% confluent monolayer of unstimulated ADSCs with or without addition of anti-human IL-1β (2ug/mL, R&D Systems) or a mouse IgG1 isotype control (2ug/mL ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human FGFs (FGF6 #238F6-025; FGF10-#345-FG-025; FGF12-2246-FG-025) were purchased from R&D systems (Minneapolis, MN).
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bioRxiv - Cell Biology 2023Quote: ... RFP labeled eEPCs were plated with monolayer and 10ng/ml of recombinant human VEGF protein (R&D System, Cat# 293-VE-010) was treated to enhance tube formation ...
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bioRxiv - Bioengineering 2023Quote: ... media was removed and frozen for secretomic screening assays for both VEGF-A (R&D Systems, Human VEGF DuoSet ELISA, Cat# DY293B) and VEGF-C (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... aspirated 1 ml of media and added 1 ml of CTL media containing 200 IU/ml human IL-2 (R&D Systems) to a final concentration of 100 IU/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... cells from each well were harvested and resuspended in 100 µL of cell resuspension solution provided with human methylcellulose enriched media (R&D systems). Each cell suspension was added to 2.5 mL methylcellulose enriched media ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat ...
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bioRxiv - Neuroscience 2023Quote: Release of cytochrome C from the mitochondria into the cytoplasm was assessed by a human cytochrome C Quantikine ELISA kit (Bio-techne R&D Systems) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... for 48 hours at which point the media was collected and analyzed for PAI-1 content using the Human Serpin E1/PAI-1 Quantikine ELISA Kit (DSE100, R&D Systems) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... They were then first incubated with blocking solution for 1 hr and then with a mouse monoclonal anti-human CCR2 antibody (MAB150-SP; R&D Systems) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: PD-L1 was detected in K562 or THP-1 EVs using the Human PD-L1 DuoSet ELISA kit and DuoSet ELISA Ancillary Reagent Kit 2 (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Plasma levels of 14 chemokines were measured using the Luminex Discovery Assay - Human Premixed Multi-Analyte Kit (R&D Systems, LXSAHM-14) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and/or 1 μmol/L ribociclib for 48 hours and then 500 μg protein lysates were assessed using human apoptosis antibody array (#ARY009, R&D systems).
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bioRxiv - Cancer Biology 2022Quote: Freshly sorted m-LSCs from primary AMLs or prepared CD34+ HSPCs isolated from normal mobilized peripheral samples were plated in human methylcellulose (R&D systems) at about 100K/ml and 2K/ml ...
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bioRxiv - Immunology 2022Quote: Llamas were immunized intramuscularly once per week over six weeks with carrier free recombinant human IL-9 or mouse IL-9 (R&D Systems). Each llama (4 in total ...
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bioRxiv - Developmental Biology 2023Quote: ... EBs were transferred into 60 mm non-adhesive dishes and differentiated in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems) for 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... were collected 24 h after infection and were kept at − 80 °C until assayed for human IL-6 (DY206-05, R&D Systems) and human IL-8 (DY208-05 ...
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bioRxiv - Cancer Biology 2022Quote: Cytokine levels produced by homotypic and heterotypic cultures were analyzed using the Proteome Profiler™ Human Cytokine Array (ARY005B; R&D SYSTEMS) kit ...
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bioRxiv - Immunology 2023Quote: ... Levels of BAFF protein were determined in culture supernatant or bone marrow plasma using the human BAFF/BLyS/TNFSF13B DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
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bioRxiv - Physiology 2023Quote: Circulating TNFR1 and TNFR2 were measured in human or mouse samples using enzyme-linked immunosorbent assay (ELISA) kits (DY255, DY726, DY425 and DY426 respectively, all from R&D Systems) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: We measured levels of secreted IL-10 in supernatants from infected or non-infected human primary macrophages cells using commercially available enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems) according to the manufacturers’ instructions.
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bioRxiv - Bioengineering 2023Quote: ... Microwells were washed once with PBS and stained for 24 hrs with 2 µg mL-1 of human pro collagen I alpha 1 antibody (AF6220, R&D Systems) and 0.848 µg mL-1 anti-LOX antibody (ab174316 ...
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bioRxiv - Genetics 2023Quote: ... The calibrator supplied with the kit by Ansh Labs is traceable to recombinant human GDF-15 from R&D Systems (Biotechne, USA). Ansh Lab ELISA Total GDF15 between batch imprecision kit controls 7.7% at 173.2 pg/ml ...
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bioRxiv - Cell Biology 2023Quote: M.tb-Lux was exposed to control- or HIV-ALF with or without (as control) supplemented human recombinant SP-D (5 μg/mL, R&D Systems). After exposure ...
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bioRxiv - Cell Biology 2023Quote: The amount of VE-Cad and Rad51 protein in the conditioned media (CM) of PMVECs or human and rat plasma samples containing EDTA was quantitated by using ELISA kit (DCADV0, R&D systems for VE-Cad and custom made by MyBiosource for Rad51 ...
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bioRxiv - Immunology 2023Quote: ... The culture was then diluted 1:50 in 1X PBS + 1% LB broth and incubated statically at 37°C with recombinant human SLPI protein (rSLPI) (CAS: 1274-PI-100, R&D Systems) at these final concentrations ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 30 minutes in 5% Bovine Serum Albumin and incubated with primary antibodies against MRC2 (1:1,200: anti-mouse: AF4789, 1μg/mL: anti-human: AF5770; R&D Systems, Bio-Techne), SEL1L (1:2,000 ...
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bioRxiv - Microbiology 2023Quote: ... anti-hCD14 magnetic beads were used and isolated cells were differentiated to human monocyte-derived macrophages (hMDMs) by addition of 50 ng/ml rhM-CSF (R&D Systems) to XVivo medium (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... MSCs were characterized based on the criteria set out by the International Society for Cellular Therapy (ISCT)(ref) using the Human Mesenchymal Stem Cell Functional Identification Kit (R&D Systems) and Human Mesenchymal Stem Cell Verification kit (R&D Systems).
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bioRxiv - Bioengineering 2023Quote: ... pulled secretome for each group was profiled using a Proteome Profiler™ Human Angiogenesis Array Kit (ARY007, R&D Systems, Minneapolis, MN) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... SLPI was measured urine samples diluted 1:20-1:200 in sterile PBS + 1% BSA using the Human SLPI DuoSet ELISA kit (CAS: DY1274-05, R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... We incubated 1.5ml of media on Protease Arrays (R&D Systems Proteome Profiler Human Protease Array Kit, Cat # ARY021B; R&D Systems Inc. ...
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bioRxiv - Genetics 2024Quote: ... TGFB2 dosage in each eye (13 controls + 2 cases) was conducted with the Human TGF-beta 2 Quantikine ELISA kit (R&D Systems), following the provided instructions.
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bioRxiv - Microbiology 2024Quote: ... This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1(R&D systems, Cat# E-522B), 2 ...
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bioRxiv - Neuroscience 2024Quote: ... SCs were then seeded on poly-L-lysine/laminin coated coverslips or petri dishes and kept in the above-mentioned medium containing recombinant human neuregulin1-ß1/heregulin1-ß1 (0.1 mg/ml, R&D systems, 396HB) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 24 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Neuroscience 2024Quote: ... and 1µM RIPK1 inhibitor [29] prior to a 4 hour incubation with 50ng/mL recombinant human TNF (R&D Systems, 210-TA).
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bioRxiv - Cancer Biology 2024Quote: ... IL-6 concentrations in tissue lysates and CAF supernatants were measured using a commercially available kit (Human IL-6 DuoSet ELISA, R&D Systems). SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Duplicates of 50 μl of concentrate were subjected to IFN-β measurement by ELISA (Human IFN-beta DuoSET ELISA, R&D Systems), after 20X upconcentration (Amicon Ultracel-10 ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... and acute phase proteins (total 36) present in the supernatants were detected with using Proteome Profiler Human Cytokines Array Kit (R&D Systems) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... then maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems). For the pilot experiment in which CD4 cells with a defined CAR BC were spiked into CD8 cells (Figure 1D-F) ...
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bioRxiv - Immunology 2024Quote: ... All primary cells were maintained in RPMI-1640 (ATCC) + 10% FBS + 100 U/mL P/S + 30 U/mL of recombinant human IL-2 (R&D Systems) at 37°C in 5% CO2.