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Citations for R&D Systems :
1551 - 1600 of 2036 citations for GITR Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: MSCs were stimulated with recombinant human IFNα (0.03 U/ml) or IFNβ (60 pg/ml) protein (R&D Systems), in combination with different concentrations of inhibitor of IFNα/β (i-IFNα/β ...
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bioRxiv - Bioengineering 2023Quote: ... and IL6 releases were analysed by Luminex® Assay using the Human Premixed Multi-Analyte Kit (R&D Systems) according to manufacturer instructions and acquiring readings with a BioPlex MagPlex Magneti Beads system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteasome activity was also measured using 5 nM of recombinant human 26S proteasome (Cat: E-365; R&D Systems).
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bioRxiv - Cancer Biology 2024Quote: ... SAA1 concentrations in patient sera were measured using a commercially available kit (Human SAA1 DuoSet ELISA, R&D Systems) and a decrease of ≥0.3 OD450 value between post and pre-surgery sera was considered significant ...
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bioRxiv - Cancer Biology 2024Quote: ... the conditioned media were assayed with Proteome Profiler Human XL Cytokine Arrays (ARY022B; R&D Systems, Minneapolis, MN, USA) performed as per manufacturer’s protocols ...
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bioRxiv - Neuroscience 2024Quote: ... cells were treated with 50 ng/mL human bone morphogenetic protein 4 (hBMP4) (R&D Systems, Minneapolis, Minnesota, USA), 50 ng/mL human vascular endothelial growth factor (hVEGF ...
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bioRxiv - Microbiology 2024Quote: ... The relative levels of cytokines were assessed using the Proteome Profiler Human XLCytokine Array kit (R&D Systems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... PDGFR-α (goat anti human, 1:300, cat. no.: AF-307-NA; RRID: AB_354459, R&D Systems, Bio-Techne), and C3 (rabbit anti human ...
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bioRxiv - Microbiology 2024Quote: ... The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h ...
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bioRxiv - Developmental Biology 2021Quote: ... the medium was switched to FE-BM1 or BM2 containing 20 ng/ml recombinant human BMP4 (rhBMP4, R&D Systems), 50 nM retinoic acid (RA ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial suspensions were incubated with human SP-D at a concentration of 10mg/ml (R&D Systems, 1920-SP-050) for 1h at RT without agitation and observed by light microscopy (Nikon Ti Eclipse equipped with a metal-oxide-semiconductor (sCMOS ...
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bioRxiv - Developmental Biology 2021Quote: ... coli-derived human N-terminal modified (C24II) fragment of Sonic Hedgehog (Shh-N; R&D Systems, Cat#1845-SH/CF) was reconstituted in PBS at 10µM and stored at -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant was collected and processed using the Proteome Profiler Human Cytokine Array Kit (R&D Systems; Cat. No. ARY005B), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... YKL-40 levels were measured using a human chitinase-3 quantikine ELISA kit (R&D systems, Inc, Minneapolis, MN 20). To reduce the skewness of phenotype distributions ...
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bioRxiv - Neuroscience 2019Quote: Human TNFα was quantified in blood serum (1:10 dilution) using a DuoSet ELISA kit (R&D Systems, Minneapolis, MN). Human IFNγ and IL10 were quantified in culture supernatants (1:2 dilution ...
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bioRxiv - Immunology 2019Quote: ... following relevant treatment with or without 200 U/ml of recombinant human (h) or mouse (m) TNFα (R&D Systems). Protein concentration was measured using the Bradford assay (Bio-Rad ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were incubated with a primary antibody of anti-Human/Mouse/Rat Vinculin mouse monoclonal (R&D systems; MAB68961-SP) diluted to 1.25 μg/mL with 0.1% BSA in PBS-0.1% Tween 20 and a secondary antibody of 2 μL/mL Alexa Fluor 488-anti mouse in 0.1% BSA in PBS-0.1% Tween 20 (Abcam) ...
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bioRxiv - Immunology 2021Quote: ... IL-11 was quantified by using a Human IL-11 Quantikine ELISA Kit according to manufacturer instructions (R&D Systems). Statistical analysis was performed on log10-transformed values with two-way ANOVA tests and Tukey correction ...
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bioRxiv - Bioengineering 2020Quote: ... IL-15 superagonist was quantified using the human IL-15/IL-15R alpha complex DuoSet ELISA (R&D Systems DY6924). For BiTE experiments with primary human T cells ...
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bioRxiv - Neuroscience 2020Quote: ... cells were fluorescent activated cell (FACS)-sorted for human alkaline phosphatase (R&D systems, Minneapolis, MN, USA, Cat N° MAB1448) and expanded further in T75 flasks (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... IL-15 concentrations were determined by ELISA according to the manufacturer’s instructions (R&D Systems Human IL-15 Quantikine Assay). Absorbance was measured at 450 nm in a Synergy H1 Microplate Reader (BioTek) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were prepared and analyzed using the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems, cat# ARY003B) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: Phospho-kinase screening was performed using a phospho-kinase array (Proteome Profiler Human Phospho-Kinase Array #ARY003B; R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The membranes were then incubated overnight at 4°C with the primary antibody dissolved in 0.5% milk in TBS-T (goat anti-human IGF2, 1:1,000, R&D Systems – AF292-NA or goat anti-mouse SOD1 ...
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bioRxiv - Physiology 2021Quote: ... CCL2 levels in urine specimens were quantified using Human CCL2/MCP-1 Quantikine® ELISA Kit (R&D systems, DCP00) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... was used as an invasion promoter and recombinant human nodal (250 ng/mL; R&D Systems, 3218-ND-025/CF) was used as an invasion inhibitor ...
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bioRxiv - Cell Biology 2022Quote: Cells were characterised using hPSC imaging kit (Human Pluripotent Stem Cell Marker Antibody Panel kit, SC008, R&D system, UK) with analysis performed on a flow cytometer (Beckman Coulter Cytomics FC 500) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured with 1000 U/mL human type I IFN-α2a (MφIFN; R&D Systems, Minneapolis, Minnesota, USA) or left untreated for 16 h.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2022Quote: ... 104 sorted CD34+ HSPCs were plated for methylcellulose-based colony forming assays [Human Methylcellulose Base Medium HSC002 (R&D Systems), which were supplemented with 2% streptomycin/penicillin ...
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bioRxiv - Microbiology 2021Quote: ... ELISA was performed using Human Gas6 DuoSet ELISA kit according to the manufacturer’s instructions (R&D Systems, Minneapolis, Minnesota, USA). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μg of mouse or human-specific IL-34 neutralizing antibody (sheep polyclonal IgG, AF5195 or AF5265, R&D Systems) was stereotaxically and bilaterally injected into the dorsal hippocampus ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933 ...
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bioRxiv - Cancer Biology 2022Quote: ... Chemokine levels were measured using the Invitrogen ELISA Kit (Quantikine mouse/human CXCL10/IP-10; R&D Systems, Minneapolis, MN) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Prostate-specific serum-free culture media contained 500 ng/mL human recombinant R-spondin (R&D Systems, Cat: 10820-904), 10uM SB202190 (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718, RRID:AB_2278695; R&D Systems, Inc., Minneapolis, MN, USA); and developed using SuperSignalTM west -pico or -femto chemiluminescence kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human WNT3A (#5036-WN-010) and WNT4 (#6076-WN-005) were obtained from R&D Systems (Minneapolis, MN, USA) and reconstituted per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-β1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng/ml human transforming growth factor-β3 (TGFβ3; cat. num. 243-B3-010/CF; R&D Systems, Minneapolis, MN), 1 µM Wnt-C59 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... BALF concentrations of TGFβ1 and TGFβ2 were measured by Human/Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit (R&D Systems) and Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit (R&D Systems) ...
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bioRxiv - Cancer Biology 2021Quote: ... the concentration of amphiregulin in the supernatant was determined using the Human Amphiregulin Quantikine ELISA Kit (R&D Systems, DAR00) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... and M2d MΦs from stimulation with and 50 ng/mL recombinant human IL-6 (R&D Systems; Minneapolis, MN, USA) and 25 ng/mL recombinant human leukemia inhibitory factor (LIF ...
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bioRxiv - Genetics 2020Quote: Plasma VEGF-A concentration was measured using the quantitative sandwich ELISA kit (Human VEGF Quantikine; R&D Systems, Minneapolis, MN) following the manufacturer’s instructions in our laboratory at low altitude (600 m) ...
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bioRxiv - Immunology 2022Quote: ... immune mediators in cell supernatants were measured using Human Custom 29-plex Multi-Analyte Kit (R&D Systems, Minneapolis, Minnesota) measuring the following analytes ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
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bioRxiv - Physiology 2022Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
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bioRxiv - Immunology 2022Quote: IL-22BP quantification was performed with the Human IL-22BP ELISA DuoSet kit (R&D System, ref DY-1087-05) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... Rat collagen I and recombinant human basic fibroblast growth factor (FGF-2) were purchased from R&D Systems (Toronto, ON). Endothelial cell growth basal medium-2 (EBM-2 ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Microbiology 2024Quote: Recombinant human IFN-β (8499-IF) and IFN-λ2 (1587-IL) were purchased from R&D Systems (Minneapolis, MN, USA). Both were reconstituted in phosphate-buffered saline (PBS ...