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Citations for Santa Cruz :
1001 - 1050 of 1083 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...
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bioRxiv - Cancer Biology 2023Quote: ... approximately 750 µg of solubilized proteins were incubated with 1 µg of anti-C/EBPβ mouse IgG (Santa Cruz Biotech, sc-7962) or normal mouse IgG (Santa Cruz Biotech ...
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bioRxiv - Immunology 2023Quote: ... 1 mg of hTCEpi cell lysate in cell lysis buffer was precleared with 25 μl of protein A/G plus agarose (SC-2003; Santa Cruz Biotechnology) for 2 hours at 4°C with rotation before the agarose was removed by centrifugation at 2,000 rcf for 10 min ...
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bioRxiv - Plant Biology 2024Quote: ... This diluted chromatin was then incubated overnight at 4°C with Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology; sc-2003) and antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and the remaining three aliquots were immunoprecipitated overnight at 4 °C with the indicated antibody mixed with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h on a nutator mixer at 4°C before Protein A/G PLUS-Agarose bead suspension (Santa Cruz, sc-2003) was added (20 µl/1 µg of antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... the total cell lysates were incubated with anti-phosphotyrosine antibody (PY99) followed by Protein A/G PLUS Agarose (both from Santa Cruz Biotechnology). The total cell lysates or the anti-phosphotyrosine immunoprecipitates were subjected to western blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... to a final volume of 1 ml and precleared by incubation with 50 μl of protein A/G-Agarose beads (Santa Cruz Biotechnology) or 20 μl of Dynabeads Protein G (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... the extracted lysates were first pre-cleared with protein G-magnetic beads at 4 °C for 2 h followed by incubation with HOXB13 antibody (2 μg/sample, Santa Cruz, sc-28333) overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...
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bioRxiv - Cell Biology 2020Quote: ... After centrifugation at 13,200 g for 15 min at 4°C, equal amounts of cell lysates were preincubated with mouse IgG (Applygene, C2118) and Protein A/G agarose beads (Santa Cruz, sc-2003) for 4 h at 4°C and then clarified by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Myc- and GFP-tagged proteins were detected by rabbit polyclonal IgG anti-GFP (FL) (1:200; sc-8334 Santa Cruz Biotechnology, Dallas, US) and mouse monoclonal IgG anti-c-Myc (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: BCR-ABL protein expression was assessed by SDS-PAGE and western blotting using a mouse anti-ABL antibody (1:1,000, Santa Cruz Biotechnology, CA, USA). Horseradish peroxidase-conjugated α-mouse antibody (1:10,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... A fraction of the mixture of protein-DNA complex was used as “input DNA.” Soluble chromatin was immunoprecipitated with anti-VDRE antibody (D-6, Santa Cruz Biotechnology Inc) and normal mouse immunoglobulin G (IgG ...
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bioRxiv - Molecular Biology 2022Quote: ... Clones were screened for gene deletion by PCR as well as LARP1 mRNA and protein depletion determined by qPCR and western blot analysis with a monoclonal LARP1 antibody (Santa Cruz sc-515873). Lesions at the locus of cutting were identified by PCR using genomic DNA to amplify the locus followed by Sanger sequencing and Synthego ICE analysis.
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bioRxiv - Developmental Biology 2022Quote: Western blotting was performed using 4–20% Mini-PROTEAN® TGX™ precast protein gels with the following antibodies anti-NFATc1 (1:1000, clone 7A6, Santa Cruz) and anti-GAPDH (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... A precleaning step of the samples was performed by adding 50 μl of Protein G-Plus agarose beads suspension (Santa Cruz Biotechnology, INC) to 1 ml of each sample (Chimera ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and identified by immunoblotting with a mouse anti-green fluorescent protein (GFP) (Santa Cruz Biotechnology, Santa Cruz, CA), or anti-Atg13 [175] antibody to detect tagged or endogenous proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1000, catalog no. sc-9996, Santa Cruz Bio-technology) and rabbit polyclonal anti-SYT1 antibody (1:5000) ...
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bioRxiv - Genetics 2020Quote: ... Section of the membrane with 75-50 kDa proteins was incubated with rat monoclonal antibodies against yeast α-tubulin (sc-53030, Santa Cruz Biotechnology) at 1:2,000 – 1:4,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blocked using either 5% BSA (Bovine Serum Albumin) in Tris buffer Saline containing 0.1% Tween (TBST) for phosphorylated proteins or 5% Blotto (Santa Cruz Biotechnology, sc-2325) for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653) in the dark at room temperature for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were resolved by SDS-PAGE and proteins were identified using immunoblotting with mouse anti-GFP antibody (Santa Cruz Biotechnology, Santa Cruz, CA) and a rabbit anti-Zwf1 (glucose-6-phosphate dehydrogenase ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by SDS-PAGE and expression was detected using the following antibodies: ATF3 (sc-81189, Santa Cruz Biotechnology, Dallas, TX, USA), cleaved caspase-3 (#9664 ...
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bioRxiv - Neuroscience 2024Quote: ... RGCs were labeled by incubation with a primary goat antibody raised against Brn3a (brain-specific homeobox/POU domain protein 3A, Santa Cruz Biotechnology, Santa Cruz, CA), 1:100 in PBS with 2% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant was transferred to a new micro-tube and precleared using 10 μl of protein A/G bead (Santa Cruz Biotechnology, sc-2003). It was then incubated with 0.5 μg of anti-Myc antibody for 1 hr at RT and incubated 20 μl of protein A bead for O/N at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... at 1:10,000 dilution and the lower portion was incubated with a mouse monoclonal antibody against histone H3 protein (Santa Cruz Biotechnologies #sc-517576) at 1:5,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... To determine interaction between BicDR1 and the viral capsid protein p24 cells were permeabilized and blocked using 3% donkey serum followed by incubation with primary antibodies targeting p24 (Santa Cruz sc-69728) and BicDR1 (Atlas Antibodies HPA041309) ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatants were immunoprecipitated with either Arc antibody (rabbit polyclonal, custom-made; Protein Tech) or normal rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) at 1 μg/500 μL lysate for 2 h at 4°C with gentle rocking ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1 mg of protein was used for Co-IP using anti-HA magnetic beads (Thermo, 88837) or Protein G magnetic beads (Thermo, 10003D) complexed with anti-Heph antibody (Santa Cruz, SC-365365) according to manufacturer’s instructions22 ...
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 1-2 μg of anti-eIF4E (sc-271480) antibody for 16 h at 4°C before protein G-PLUS-agarose slurry (Santa Cruz sc-2002) was added for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Nucleus proteins of the ANKRD49-OE or LV5 H1299 cells (1×106) were extracted and 25 µg of them were precleared using Protein A/G Agarose (Santa Cruz, California, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: Cell lysates (500 µg) were pre-cleared with 30 µl of protein A/G plus-agarose beads (Santa Cruz Biotechnology, catalog #: SC-2003) and 1.0 µg of normal mouse IgG (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... The epitope-tagged Pum and α-tubulin proteins were detected by successive incubation with anti-Myc 9E10 monoclonal primary (SC-40, Santa Cruz Biotechnology) and anti-α-tubulin B-5-1-2 monoclonal primary (T5168 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µg of protein were first separated by SDS-PAGE and then blotted with the following antibodies: MSH3 (1:500, Santa Cruz, sc-271080), huntingtin (1:2,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining supernatant was subjected to immunoprecipitation using 15 μl of mouse monoclonal antibody against green fluorescent protein (catalog no. sc-9996, Santa Cruz, CA, USA). The immunoprecipitate was gently shaken overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections underwent permeabilization with triton-x and were incubated overnight at 4 degrees with 1:500 of goat raised glial fibrillary acidic protein primary antibody (GFAP; SC-6170; Santa Cruz Biotechnologies, Santa Cruz, CA). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: LDLR cell surface protein was quantified by fixing cells in 4% paraformaldehyde and incubated with anti-LDLR at 1:100 (Santa Cruz Biotechnology, sc18823) for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was processed for DREADDs expression validation and selected tissue was stained for antibody against striatal-enriched protein tyrosine phosphatase (STEP, mouse, dilution 1:500, Santa Cruz sc-23892).
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bioRxiv - Neuroscience 2024Quote: ... Co-IP was performed using anti-PNKP (BioBharati Life Science, BB-AB0105) antibody with Protein A/G PLUS agarose beads (Santa Cruz Biotechnology, sc2003,) overnight ...
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FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5bioRxiv - Neuroscience 2024Quote: ... beads were resuspended in Laemmli sample buffer and protein were eluted by boiling 10 minutes at 90°C and analyzed by SDS-PAGE and immunoblotting using FUS (Santa Cruz sc-47711), ETV5 (Proteintech 13011-1-AP ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 500 μg of the supernatant proteins were incubated with 2 μg of mouse monoclonal antibody raised against SNAP-25 (Santa Cruz, sc-390814) or Syntaxin1a (MilliporeSigma ...
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bioRxiv - Cell Biology 2020Quote: ... heat shock protein 90A/B (HSP90) was detected using a rabbit polyclonal antibody (H-114, 1:2500 dilution, Santa Cruz Biotechnology, Santa Cruz, CA, USA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were incubated with the antibodies for 1h at 4°C on a roller before 80µl Protein G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX, USA) were added and samples were incubated overnight at 4°C on a roller ...
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bioRxiv - Synthetic Biology 2021Quote: ... Blots were then washed three times for 15 minutes at 4 °C with 0.05% TBS-Tween and stained for 4 hours with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) (Santa Cruz Biotech, sc-516102) at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 10% BSA was used to block the membrane followed by protein detection using primary antibody of 1:1000 dilution such as anti-β actin (C4, SC-47778, mouse monoclonal antibody, Santa Cruz Biotechnology, USA), anti-GFP (B-2 ...
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bioRxiv - Microbiology 2021Quote: ... followed by incubation with a rabbit polyclonal anti-VP1 antibody (Ma, et al., 2020) or anti-guanylate binding protein (GBP) 1–5 (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing ...