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Citations for Santa Cruz :
901 - 950 of 1083 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Protein bands detected by incubation for12-16h at 4°C with polyclonal primary antibodies to GAPDH(sc-32233, Santa Cruz), USP21(ab171028 ...
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lysates were incubated with 20 µL pre-coupled antibody-protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 3 hr at 4 °C rotating ...
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bioRxiv - Microbiology 2022Quote: ... Bead-based immunoprecipitation was performed with protein G Dynabeads and IgG anti-BrdU antibody IIB5 (Santa Cruz Biotechnology, SC-32323). RNA was extracted and RT-qPCR performed as previously described.
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bioRxiv - Biochemistry 2022Quote: ... The proteins were separated on SDS-PAGE gels and analyzed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Biochemistry 2022Quote: ... and the expression of both G3BP1-FRB and FKBP-hADAR(E488Q) proteins was confirmed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10 ...
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bioRxiv - Microbiology 2022Quote: ... Total MAPK proteins were detected using a commercial mouse monoclonal anti-Mpk1 antibody (sc-165979, Santa Cruz Biotechnology, Heidelberg, Germany), a custom-designed polyclonal anti-Fus3/anti-Fmk1 antibody (SICGEN Research and Development in Biotechnology Ltd ...
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bioRxiv - Molecular Biology 2022Quote: ... Hedgehog pathway protein localization coverslips were incubated with either 1) mouse anti-SMO (1:50, Santa Cruz Biotechnology, sc-166685) and rabbit anti-ARL13B (1:800 ...
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bioRxiv - Neuroscience 2024Quote: Whole-cell protein lysates were prepared from hCO or enriched astrocytes using RIPA lysis buffer (Santa Cruz Biotechnology, sc-24948A) within HypoxyLab after 48 hr of hypoxia exposure ...
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bioRxiv - Biochemistry 2024Quote: ... and as secondary antibody 1:5000 diluted mouse IgG kappa binding protein conjugated to Horseradish Peroxidase (Santa Cruz, sc-516102). Nitrocellulose membranes (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... as a primary and mouse IgG κ-binding protein horseradish peroxidase (1:50,000 dilution; Santa Cruz Biotechnology, Dallas, United States) as a secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by SDS–polyacrylamide gel electrophoresis and detected with the following antibodies: ARF6 (Santa Cruz Biotechnology sc-7971), ASAP1 (Santa Cruz Biotechnology sc-81896) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by SDS– polyacrylamide gel electrophoresis and detected with the following antibodies: Vinculin (Santa Cruz Biotechnology sc-25336), Tubulin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were precleared for 1h at 4°C with the addition of Protein A/G PLUS-Agarose (Santa Cruz Biotechnologies). Chromatin was immunoprecipitated overnight at 4°C using antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated in 10% SDS–PAGE gels and transferred onto a nitrocellulose membrane (Santa Cruz Biotech, Dallas, TX, USA). Membranes were blocked in 5% w/v nonfat dry milk in 1x Tris-buffered saline (TBS ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated for 1 hour with a Yes-associated protein (YAP) primary antibody (Santa Cruz Biotechnology, sc101199, 1:200) followed by secondary antibody labeling ...
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bioRxiv - Physiology 2024Quote: ... 50 μg of total protein extraction was used for semiquantitative analysis of CLDN5 (Santa Cruz Biotechnology, sc-374221, TX, USA). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... and a 1:100 dilution of a mouse monoclonal antibody to the ribosomal protein L4 (Sc-100838 Santa Cruz Biotechnology) in a blocking solution at 4° C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Each hippocampus was weighed and transferred into 500 μl of ice-cold lysis buffer (NPER, Neuronal Protein Extraction Reagent, ThermoScientific) containing sodium orthovanadate (0.5 mM, Santa Cruz), phenyl-methylsulfonyl fluoride (PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... Pan anti-Kbhb antibody was first conjugated to Protein A/G plus agarose (Santa Cruz Biotechnology Inc, Cat# sc-2003) and then incubated with tryptically digested peptides with gentle agitation overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... PVDF membranes with transferred proteins were incubated with the anti-Tau primary antibodies: 0.2 μg/mL Tau13 (sc-21796 AF680, Santa Cruz) or 0.2 μg/mL AT8 (ab64193 ...
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bioRxiv - Cancer Biology 2021Quote: ... Separated proteins were transferred to nitrocellulose membranes and immunoblotted with primary antibodies specific for PAK3 (Santa Cruz sc-1871, 1:200), c-Jun (CST #9165 ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL of magnetic Protein G Dynabeads were coated with 4 g of anti-GATA4 (G4) antibody (sc-25310X, Santa Cruz) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies specific for the following proteins were obtained from the indicated suppliers: CD81 (1:1,000, Santa Cruz Biotechnology, Dallas, TX, USA), CD9 (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... 3 μg each) or isotype control antibodies (rabbit IgG or mouse IgG, respectively) were coupled to protein A/G agarose beads (Santa Cruz) in coupling buffer (0.01M sodium phosphate ...
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bioRxiv - Cancer Biology 2022Quote: ... After centrifugation at 13,200 rpm for 20 minutes at 4°C a pre-clearing step was performed by adding 25 µl of Protein A/G PLUS-Agarose beads (Santa Cruz) and incubating the samples for 1 hour at 4°C in rotation ...
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bioRxiv - Immunology 2021Quote: ... total protein was extracted by lysing tissue in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (Santa Cruz #sc-24948), sodium orthovanadate ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996, Santa Cruz). Western blotting (Fig ...
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bioRxiv - Developmental Biology 2020Quote: ... The co-IP was perform by incubating extract with protein A or G Dynabeads conjugated with antibodies against PRDM9 (custom-made) or EWSR1 (sc-6532, Santa Cruz) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and lysates were precleared with salmon sperm DNA/protein A agarose slurry for 1h and incubated with rabbit IgG (Santa Cruz) or SREBP1 antibody in the presence of protein A agarose beads overnight ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were transferred into new tubes after centrifugation for 10 min at 14,000g and further incubated with the indicated antibodies for 3 hours at 4 □ followed by the addition of protein A/G beads (Santa Cruz), or with Anti-Flag magnetic beads (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by the addition of protein A/G agarose beads (20 μL per sample; Santa Cruz Biotechnology, sc-2003) and incubation overnight at 4 °C with rocking ...
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bioRxiv - Plant Biology 2020Quote: ... TPR2-myc protein expression was verified by western blot using c-Myc antibody sc-789 (Santa Cruz Biotechnology, Dallas, TX, USA).
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bioRxiv - Physiology 2020Quote: ... After the blots had been incubated with a HRP-labeled mouse IgGκ light chain binding protein (1:5000, sc-516102, Santa Cruz), they were reacted with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were separated by SDS-PAGE and immunoblotting was performed as described above with anti-BAP1 antibody (Santa Cruz: sc-28383).
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed under conditions in which only covalent interactions were preserved with anti-GFP (mouse) antibody overnight at 4°C following which Protein A agarose beads (Santa Cruz) were used to precipitate the antibody bound complex.
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bioRxiv - Cell Biology 2020Quote: Total protein from cultured cells was homogenized in RIPA lysis buffer with a mixture of protease and phosphatase inhibitors (Santa Cruz). Protein concentration was determined using the Bradford assay (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... One mg of total protein of each sample were immunoprecipitated with 50 ul of p53 antibody (D0-1, sc-126, Santa Cruz) and incubated overnight on rotating wheel at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed and preincubated with Protein A/B PLUS agarose beads (Cat# sc-2003, Santa Cruz Biotech, Dallas, TX, USA) for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the remaining protein fraction and kept at 4 °C for 2 hrs on a rotating apparatus followed by overnight incubation with 30 μl of the protein G plus agarose beads (#SC-2002, Santa Cruz) at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The following HRP-conjugated secondary antibodies or proteins were used for development of western blots at 1:5000 – 1:10000: mouse IgGκ-binding protein (m-IgGκ BP-HRP) (sc-516102, Santa Cruz); mouse monoclonal anti-rabbit IgG-HRP (sc-2357 ...
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bioRxiv - Immunology 2021Quote: ... One mg of cell lysate in 500 μL lysis buffer was pre-cleared by adding 10 μL of protein A/G PLUS-agarose beads (Santa Cruz) and incubating for 1 hour at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the detection of CDK4 proteins via a quick WB procedure using the sc-601 primary antibody from Santa Cruz Biotech ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and then the protein concentration of each sample was determined by bicinchoninic acid assays (Santa Cruz Biotechnology). Diluted sample and reaction buffer were added into 96-wells plate containing menadione or isoplumbagin with cofactor NADH and water-soluble tetrazolium salt [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium] reagent ...
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bioRxiv - Cell Biology 2020Quote: ... Chromatin was pre-cleared by O/N incubation with 40 μL of Protein A/G beads (sc-2003, Santa Cruz biotechnology) on a stirring wheel at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... equal amounts of protein of cleared lysate were incubated with anti-GFP polysera (1:1,000) overnight prior to addition of protein-G agarose beads (Santa Cruz Biotechnology) for an additional 2 hr ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were prepared from microdissection of 10 hippocampi of juvenile mice incubated in RIPA lysis buffer (Santa Cruz sc-24948). Active Rac1 Detection was performed using Active Rac1 Detection Kit of Cell Signalling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... for EGFP-tagged Vpu/F1/F2 detection, Nef antibody (EEH1, NIH AIDS Reagent Program) and anti-HA antibody for HA-tagged BST-2 protein (Santa Cruz). Secondary antibodies conjugated to HRP (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... INSR was immunoprecipitated by incubating 500 μg of protein lysate with 2 μg INSR antibodies (Rabbit pAb, Cat. #sc-711, Santa Cruz) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Different primary antibodies were used for the detection of interest proteins: anti-IL-1β rabbit polyclonal (1:1000, H-153, SC-7884, Santa Cruz), anti-caspase-1 (p20 ...