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Citations for Santa Cruz :
951 - 1000 of 1083 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and subsequently incubated with primary antibodies rabbit anti-Prosurfactant Protein C (pro-SPC) (MilliporeSigma, Burlington, MA, USA, #AB3786, RRID: AB_91588) and mouse anti-Acetylated α Tubulin (ACT) (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... For co-localization studies employing confocal microscopy the following antibody combinations were used: mouse anti-non-structural protein 1 (Santa Cruz)/goat anti-mouse Alexa568 (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were stripped and re-probed with a polyclonal antibody directed against the immunoprecipitated GRK2 protein (C15, Santa Cruz Biotechnology Inc). Blots were developed using a chemiluminescent method (ECL ...
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bioRxiv - Neuroscience 2023Quote: ... anti-S6 ribosomal protein (S6) (mouse monoclonal clone C-8, #sc-74459, RRID: AB_1129205, Santa Cruz Biotechnology; 1:1000 for WB), anti-4E-BP1 (rabbit monoclonal clone 53H11 ...
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bioRxiv - Plant Biology 2022Quote: ... The following primary antibodies were used for detection of epitope-tagged proteins: mouse monoclonal anti-GFP clone B-2 (1:1,000, catalog no. sc-9996, Santa Cruz Biotechnology) and anti-RFP antibody (1/2000 ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting immune complex was then incubated for 1 h with 40 µl of protein A/G plus agarose beads (sc-2003, Santa Cruz). After this ...
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bioRxiv - Cell Biology 2023Quote: ... 50 µl DynabeadsTM Protein G beads were first coated with 1 µg mouse anti-MYC antibody (sc-40, Santa Cruz Biotechnology) or normal mouse IgG1 (sc-3877 ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 4 °C) and then pre-cleared with 20 μl of protein A/G PLUS agarose beads (sc-2003, Santa Cruz Biotechnology) for 1 hour at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... supernatant was incubated with anti-NLRP3 or anti-ZDHHC7 antibody as indicated and Protein A/G conjugated agarose beads (Santa Cruz) to immunoprecipitate endogenous NLRP3 or ZDHHC7 proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... 300 μg nuclear extracts diluted in 500 μL IP lysis buffer was pre-cleared by incubating with 20 μL protein A/G agarose beads (Santa Cruz) for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000 µg of total protein suspended in 500µL of cell lysate was precleared with 20 µL of protein A/G plus agarose beads (Santa Cruz Biotechnology) and 1 µL of normal rabbit IgG (Cell Signalling ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were cleared by centrifugation at 13000rpm for 15min at 4°C and extracts of equal amount of total protein were used to immunoprecipitated p53 using 2 μg of antibody (DO-1, Santa Cruz) and 20 μL of protein A magnetic beads (prewashed with lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Lysis supernatants were migrated in SDS-gels and used for an immunoblot to detect P53 protein with p53 antibody (Pab 1801): sc-98 (Santa Cruz).
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bioRxiv - Microbiology 2024Quote: ... 1 μg envelope plasmid pMDG encoding VSV-G protein and 1.5 µg lentiviral vector plasmid encoding shRNA to MSI-2 (sc-75834-SH; Santa Cruz Biotechnology). A lentiviral vector plasmid encoding scrambled shRNA sequences with no cellular targets was used as the negative control (sc-108060 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 48 h at 4 °C with a goat anti-glial fibrillary acidic protein (GFAP) antibody (1:300; Santa Cruz) or a rabbit anti-ionized calcium-binding adapter molecule/allograft inflammatory factor 1 (IBA1/AIF-1 ...
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bioRxiv - Biochemistry 2024Quote: One million cells were washed once with PBS and extracted with 100 ul protein extraction RIPA buffer and protease inhibitors from Santa Cruz Biotechnology (SCTB ...
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bioRxiv - Bioengineering 2024Quote: ERBB2 gene (encoding HER2 protein) was knocked out using CRISPR/Cas9 KO and HDR (Homologous directed recombination) plasmids from Santa Cruz biotechnology (37) ...
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bioRxiv - Genetics 2024Quote: ... Protein extracts from transfected cells were analyzed by western blotting using commercial primary antibodies (Sigma anti-FLAG M2 F1804, Santa Cruz anti-GFP SC9996 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed three-times with TBST (TBS containing 0.1% Tween-20) and incubated with HRP-conjugated mouse IgG kappa binding protein (1:5000; Santa Cruz Biotechnology, USA) for 2 hrs at room temperature ...
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bioRxiv - Immunology 2021Quote: ... pre-cleared with protein A agarose beads (Pierce) and incubated with agarose conjugated anti-Rad52 mAb (clone F-7; sc-365341 AC, Santa Cruz Biotechnology) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... samples containing same protein amount were subjected to immunoprecipitation with rabbit polyclonal antibody against PDE4B (H-56) (#sc-25812, Santa Cruz Biotechnology) coupled to GammaBind Plus Sepharose beads (# 17-0886-02 ...
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bioRxiv - Microbiology 2021Quote: ... with the lysate overnight at 4 °C followed by addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, Dallas, TX) and incubation for 2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18 ...
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bioRxiv - Neuroscience 2020Quote: ... brains were incubated overnight with combinations of the following primary antibodies: rabbit anti-protein kinase C (anti-PKC) clone C20 (1:1000; Santa Cruz Biotechnology), rabbit anti-phospho-histone H3 (1:2500 ...
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bioRxiv - Cancer Biology 2020Quote: ... were cleared by incubating with 2.0 µg of the control IgG and 20 µL vortexed Protein G PLUS-Agarose beads (Santa Cruz, sc-2002) for 30 min with rocking at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Thirty micrograms of total protein were separated by SDS-PAGE and probed with primary antibodies against NFATC2 (Santa Cruz Biotechnology, sc-7296), NFATC4 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362; GRK5: Santa Cruz, sc-518005 ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture supernatants of cultured cerebellar neurons were incubated overnight with Reelin antibody G10 and Protein A/G beads (Santa Cruz Biotechnologies) or brain homogenates were resuspended in immunoprecipitation buffer (25mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: Cell lysates (500 μg) were pre-cleared with 30 μl of protein A/G plus-agarose beads (Santa Cruz, catalog #: sc-2003) and 1.0 μg of normal mouse IgG antibody (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used for Western blot of β-actin and AK2 proteins were: anti-actin (Santa Cruz Biotechnology sc-47778, 1:1000), anti-AK2 (ProteinTech 11014-1-AP ...
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bioRxiv - Plant Biology 2021Quote: ... the protein-retained beads were boiled in Laemmli buffer and immunoblotted using anti-GST and anti-MBP antibodies (Santa Cruz Biotechnology, USA).
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bioRxiv - Cancer Biology 2021Quote: ... to detect DARPP-32 (Cat no.: sc-135877; Dilution 1:200) and α-Tubulin (Cat no.: sc-5286; Dilution 1:1000) protein were obtained from Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... protein lysates (500 μL of NP-40 buffer containing 200 μg of protein) were incubated with 10 μg of primary antibody Neu (F-11) agarose conjugate (sc-7301; Santa Cruz Biotechnology) overnight at 4°C with gentle rotation ...
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bioRxiv - Bioengineering 2020Quote: Tissue sections were incubated overnight with polyclonal rabbit antibodies raised against c-fos protein (1:1000; Santa Cruz Biotechnology Inc.; sc-253), GFAP (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... CBLB and GAPDH in the protein blots were probed with anti-CBLB- and anti-GAPDH-antibodies (SC-8006 and SC-166545, respectively; Santa Cruz Biotechnology). Flow Cytometry analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were pre-cleared with control IgG for 1 hr at 4 °C and Protein A/G Plus-Agarose beads (Santa Cruz Biotechnology) for 30 min at 4 °C before incubation with primary antibody overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cbl was immunoprecipitated from 20-50 mg of total protein from each treatment using anti-Cbl AB coupled to agarose beads (sc-170 AC, Santa Cruz Biotechnology). The immunoprecipitated proteins were eluted from Cbl AB-agarose beads with Pierce IgG Elution Buffer (21004 ...
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bioRxiv - Immunology 2022Quote: ... WCL were incubated with GLUT3 antibody overnight and then with Protein A/G PLUS-Agarose bead slurry (Santa Cruz Biotechnology, sc-2003) for 3h ...
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bioRxiv - Neuroscience 2023Quote: ... Immunodetection of ubiquitinated proteins was accomplished by incubating the membrane overnight with 1:1000 diluted monoclonal ubiquitin antibody P4D1 (sc-8017; Santa Cruz Biotechnology) and exposing the membranes to peroxidase-conjugated anti-rabbit secondary antibody and ECL Advance chemiluminescent substrate (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30 °C for 30 minutes or 1 hour before separation by SDS–PAGE followed by immunoblot analysis for MBP-substrate proteins (Anti-MBP-probe Antibody (R3.2) (Santa Cruz sc-53366)).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... Precleared extracts were incubated with 40 μL of 50% protein A/G agarose and 5 of normal rabbit IgG (Santa Cruz Biotech), H3K27ac (Active Motif ...
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bioRxiv - Immunology 2024Quote: ... Membranes were incubated in TBST containing 5% (w/v) skimmed milk with mouse IgG kappa binding protein (m-IgGκ BP) conjugated to HRP (1:2000) (sc-516102, Santa Cruz Biotechnology) for 3h at 4°C on a rotary and washed thrice with TBST for 10 min each time ...
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bioRxiv - Genomics 2023Quote: ... Samples were pre-cleaned by incubating with 2–4 μg of normal immunoglobulin G (IgG) and 50 μl of A / G-agarose protein microspheres (Santa Cruz Biotechnology) for 1 hour at 4 °C with agitation for preclearance ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were immunoprecipitated using monoclonal mouse anti-WWOX cocktail antibodies53 together with protein A/G plus agarose beads (Santa Cruz, Texas, USA) and incubated overnight at 4°C on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Clarified lysates were incubated with the appropriate antibodies for 2h at 4°C and subsequently with protein A/G agarose beads (Santa Cruz Biotechnology) for 1h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: The total proteins were extracted by lysing the cells with radioimmune precipitation assay (RIPA) buffer (Santa Cruz Biotechnology, Santa Cruz, CA, USA) that contained protease inhibitor for 30 min on ice followed by centrifuging at 4 C ...