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Citations for Santa Cruz :
701 - 750 of 1083 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The trimmed reads were mapped to the hg19 assembly of the human genome (University of California at Santa Cruz, version from iGenomes) using STAR v2.5.3a (Dobin et al. ...
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bioRxiv - Immunology 2020Quote: ... they were incubated with a 1% BSA solution in 1X PBS containing primary antibody (rabbit polyclonal IgG against human LC3, 1:1000 dilution, Santa Cruz Biotechnology) for one hour at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 80% confluent RHMVECs in 6-well plates were transduced with human T111I EL (pKS18) lentiviruses with 4 μg/ml Polybrene (#134220; Santa Cruz Biotechnology) in a total volume of 1 ml DMEM ...
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bioRxiv - Cell Biology 2022Quote: ATDC5 and SaOS-2 cells were transfected with the mouse or human SHIP2 double nickase plasmid (Santa Cruz, sc-421138-NIC (mouse), sc-401622-NIC (human) ...
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bioRxiv - Immunology 2023Quote: ... The assay for CLCA1 was performed using rabbit anti-human CLCA1 (amino acid 33-63) and HRP-conjugated goat anti-rabbit IgG antibody (Santa Cruz Biotechnology) as described previously (12) ...
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bioRxiv - Cancer Biology 2024Quote: Rab27B was knocked out in the human colon cancer cell line HCT116 using Rab27B CRISPR/Cas9 KO plasmid (sc-403498; Santa Cruz Biotechnology), followed by insertion of a homology-directed repair (HDR ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... The mouse anti-human SOX18 primary antibody used for immunostaining (E-11) was validated in HemECnaïve and HemECshSOX18 (1:100, Santa Cruz, sc-376166), Cells were co-stained with a rabbit anti-human VE-Cadherin (1:100 ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... and blocked for 30 minutes in 10% donkey serum followed by incubation with mouse anti-human SOX18 (1:50; Santa Cruz Biotechnology), rabbit anti-human SREBP2 and UEA1 fluorescently labeled with Alexa Fluor 649 (1:50 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was transferred to a new tube and prepared for immunodetection with an anti-human PCPE-1 antibody (1:25; catalog sc-73002; Santa Cruz Biotechnology) with Protein Simple Jess/Wes capillary-based system (Bio-Techne ...
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bioRxiv - Evolutionary Biology 2023Quote: ... sc-15179 (Goat polyclonal) at 1:400 dilution for zebrafish tissue and 1:100-1:400 dilution for human tissue (Santa Cruz Biotechnology), Cytokeratin 5/6 (mouse monoclonal ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Uroplakin 1a (N-16):sc-15170 (Goat polyclonal) at 1:400 dilution for zebrafish tissue and 1:200 dilution for human tissue (Santa Cruz Biotechnology), Uroplakin 2 (k-18) ...
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bioRxiv - Microbiology 2024Quote: ... CH58 and CH59 were used as primary antibodies at 1:5000 dilution in PBS and rabbit anti-human Ab HRP conjugate (Santa Cruz Biotechnology) was used as secondary antibodies at 1:10,000 dilution in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with one of the following anti-human primary antibodies diluted in blocking buffer: goat PAX3 (1:100; 4°C overnight; Santa Cruz, sc-34916), mouse TYRP1 (MEL-5 ...
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bioRxiv - Genomics 2022Quote: ... The media on human RPTEC was then changed to the fresh lentiviral supernatants supplemented with polybrene (0.5 µg/ml, Santa Cruz Biotechnology; sc-134220) and cultured for 24 h ...
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bioRxiv - Genomics 2021Quote: ... The media on human RPTEC was then changed to the fresh lentiviral supernatants supplemented with polybrene (0.5 μg/ml, Santa Cruz Biotechnology; sc-134220) and cultured for 24 h ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Slides were washed in dPBS three times and stained with primary antibodies: goat anti-human neutrophil elastase (Santa Cruz Biotech 9520, 1:1000), and goat anti-human myeloperoxidase (Abcam 9535 ...
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bioRxiv - Physiology 2023Quote: ... Histological sections were incubated overnight at 4°C with the primary antibodies (rabbit anti-human COX1, ab133319, abcam, 1:200; rat anti-mouse Endomucin, sc-5495 Santa Cruz, 1:200), then incubated with Texas Red-conjugated goat anti-Rat (TI-9400 Vector Labs ...
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bioRxiv - Molecular Biology 2022Quote: ... human primary SCLC tumor specimens and genetic engineered mouse model (GEMM) tumor specimens with anti-NKX2-1 (1:100; Santa Cruz, sc-53136), anti-SOX1 (R&D ...
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bioRxiv - Genetics 2022Quote: ... anti-rabbit αß-crystallin (Enzo, ADI-SPA-223), anti-rabbit DNAJB4 (proteintech, 13064-1-AP) and anti-human DNAJB4 (Santa Cruz, sc-100711). Rhodamine Phalloidin Reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with a pool of three CRISPR/Cas9 plasmids containing gRNA targeting human FKBP5 and a GFP reporter (Santa Cruz, sc-401560). 48 hours post transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies against the following proteins were used: GAPDH (Santa Cruz Biotechnology, Inc, Dallas, TX, USA); Bcl2 (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... the soluble proteins from whole cell lysate were immunoprecipitated with anti-Kaiso (sc-365428, Santa Cruz) or anti-GFP (ab290 ...
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bioRxiv - Cancer Biology 2020Quote: ... and the supernatant was precleared by incubation with protein A/G beads (Santa Cruz Biotechnology Inc.) on a rotating platform at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Immune complexes were collected using protein A/G PLUS-Agarose beads (sc-2003, Santa Cruz Biotechnology), washed once with LiCl2 buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were precleared with protein A/G-Agarose (Santa Cruz Biotechnology, Santa Cruz, CA) by incubation for 1 h at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2021Quote: ... The cell lysates were precleared with protein A/G-Agarose (Santa Cruz Biotechnology, Santa Cruz, CA) by incubation for 1 h at 4 °C with constant agitation ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were extracted using a RIPA lysis buffer containing protease and phosphatase inhibitors (Santa Cruz). Subcellular organelle fractionation was performed using a Mitochondrial Isolation Kit (Qiagen Qproteome #37612 ...
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bioRxiv - Plant Biology 2021Quote: ... GST-tagged proteins were detected with rabbit polyclonal GST (Z-5): sc-459 (Santa Cruz Biotechnology) at a dilution of 1:5,000 ...
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bioRxiv - Plant Biology 2021Quote: ... Protein samples were separated using SDS-PAGE and immunoblotted with α-GFP-HRP (Santa Cruz, USA), α-FLAG (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with the following antibodies: α-RSL24D1 (dilution 1:1,000; Santa Cruz sc-100840), α-RPA194 (dilution 1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... N-protein staining was followed by incubation with rabbit anti-NFkB (p65) (sc-372, Santa Cruz) or rabbit anti-IRF3 (sc-9082 ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were detected with a primary antibody to PGC-1α (Santa Cruz Biotechnology, #sc-518025), α-tubulin (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2020Quote: ... bound to 20 μl of Protein G-plus Agarose (Santa Cruz Biotechnology, Cat. No. sc-2002), and then 4x washed with 1 ml Lysis buffer ...
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bioRxiv - Physiology 2022Quote: ... Equal protein loading was verified using antibody against β-actin (Santa Cruz, sc-47778, 1:15,000). Secondary antibodies were ...
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bioRxiv - Physiology 2020Quote: ... Antibody-HCN4 complexes were immunoprecipitated with protein A/G PLUS-agarose (Santa Cruz; Cat. #SC-2003) and washed 1x with lysis buffer and 2x with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... for 60 min at 4°C and incubated with protein A/G-Sepharose (Santa Cruz Biotechnology) for additional 60 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies to the p15 (sc-612) and p300 (sc-585) proteins were purchased from Santa Cruz. Antibodies to SUZ12 (Ab12073) ...
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bioRxiv - Immunology 2021Quote: ... the supernatants were collected and incubated with Protein A / G Sepharose beads (SC-2003, Santa Cruz) coupled to specific antibodies overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 µl of Dynabeads coupled to Protein A or Dynabeads Pan mouse IgG (Santa Cruz Biotechnology) were added ...
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bioRxiv - Cell Biology 2021Quote: ... and 25 μL of protein-A/G agarose beads (#sc-2003, Santa Cruz Biotechnology, Dallas, TX) was added to each reaction and rotated for two h at 40C ...
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bioRxiv - Microbiology 2023Quote: ... GFP-tagged protein was detected using the B2 anti-GFP HRP-conjugated antibody (Santa Cruz Biotechnology), and HA-tagged proteases were detected using the anti-HA peroxidase-conjugated antibody (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were detected using Western Blotting Luminol Reagent (Santa Cruz Biotechnology, cat. no. sc-2048) at the MicroChemi high performance imager (DNR Bio-Imaging Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... The soluble chromatin was precleared with Protein A Plus agarose beads (sc-2001, Santa Cruz Biotechnology) at 4 ℃ for one hour and then incubated with anti-FOXK2 (ab5298 ...
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bioRxiv - Cancer Biology 2024Quote: ... The soluble chromatin was precleared with Protein A Plus agarose beads (sc-2001, Santa Cruz Biotechnology) at 4°C for one hour and then incubated with anti-MYCN or anti-c-MYC antibodies or with control mouse IgG at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... GAPDH proteins were also labeled with anti-GAPDH Rabbit IgG (Santa Cruz, sc:25778, 1:2,000). Horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... Whole cell protein lysates for samples were prepared using RIPA buffer system (Santa Cruz, sc-24948). Protein concentration was quantified using the bicinchoninic acid assay (Pierce ...
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bioRxiv - Cell Biology 2024Quote: ... EDTA-free Protease Inhibitor Cocktail Tablet was from Roche (catalog no. 11836170001). Protein A/G PLUS-Agarose (Catalog no. sc-2003) was from Santa Cruz Biotechnology Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4 degrees before the addition of equilibrated protein G agarose beads (Santa Cruz, sc-2002).
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bioRxiv - Immunology 2023Quote: ... the media contained protein synthesis inhibitors at the following concentrations: 5μg/mL HAR (Santa Cruz Biotechnology), 25μg/mL EME (Calbiochem) ...
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bioRxiv - Cancer Biology 2023Quote: ... protein expression was visualized using chemiluminescence Luminol reagent (SC-2048, Santa Cruz Biotechnology, Dallas, TX, USA) and film exposure (45-001-508 Amersham Hyperfilm ECL Film ...