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• A novel SATB1 protein isoform with different biophysical properties

  Tomas Zelenka, et al., bioRxiv - Molecular Biology 2023

  Quote: For the co-localization immunofluorescence experiments analyzed with confocal microscopy (Supplementary Figure 1f) we utilized a commercially available antibody against SATB1 detecting both short and long isoforms (1:100; Santa Cruz Biotechnology, sc-376096) and our custom-made polyclonal antibody (1:500 ...
• A novel SATB1 protein isoform with different biophysical properties

  Tomas Zelenka, et al., bioRxiv - Molecular Biology 2023

  Quote: ... was kept and the immunodepleted material was subjected on a second immunoprecipitation reaction utilizing an antibody detecting epitopes on both long and short SATB1 isoforms (Santa Cruz Biotechnology, sc-376096). The material from the second immunoprecipitation reaction was kept (sample 3) ...
• A novel SATB1 protein isoform with different biophysical properties

  Tomas Zelenka, et al., bioRxiv - Molecular Biology 2023

  Quote: ... 50 μl of beads pre-blocked with 15 μg rabbit anti-SATB1 or rabbit IgG sera (Santa Cruz Biotechnology, C2712) at 4°C for 4 hours rotating and crosslinked at room temperature for 30 minutes with tilting/rotation using cross-linker Bis(sulfosuccinimidyl ...
• The apicoplast is important for the viability and persistence of Toxoplasma gondii bradyzoites

  Syrian G. Sanchez, et al., bioRxiv - Microbiology 2023

  Quote: ... for apicidin-induced differentiation tachyzoites were used to infect host HFFs and after 24h they were treated with 40 nM apicidin (Santa Cruz) for up to 6 days ...
• A novel SATB1 protein isoform with different biophysical properties

  Tomas Zelenka, et al., bioRxiv - Molecular Biology 2023

  Quote: ... The membrane was blotted using SATB1 antibodies (custom-made anti-long in a 1:200 dilution and Santa Cruz sc-376097 in a 1:200 dilution) or anti-GFP (Minotech Biotechnology ...
• A novel SATB1 protein isoform with different biophysical properties

  Tomas Zelenka, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Samples were blocked for 30 minutes with Blocking Buffer as previously described and stained with an anti-SATB1 antibody targeting all isoforms (Santa Cruz Biotechnology, sc-5990) and a donkey anti-goat Alexa Fluor 546 secondary antibody ...
• Extracellular Delivery of Functional Mitochondria Rescues the Dysfunction of CD4⁺ T Cells in Aging

  Colwyn A. Headley, et al., bioRxiv - Immunology 2023

  Quote: ... Membranes were washed and incubated with mouse IgG kappa-binding protein conjugated to horseradish peroxidase (m-IgGk-HRP, 1:4000) (Santa Cruz technologies) in 5% milk for 1 h at 4°C ...
• UBAP2L drives scaffold assembly of nuclear pore complexes at the intact nuclear envelope

  Yongrong Liao, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-Nup133 (E-6) (Santa Cruz Biotechnology, sc-376763 AF488), mouse monoclonal anti-TIA-1 (Santa Cruz Biotechnology ...
• Hepatitis B virus x (HBx) protein increases cellular OCT3/4 and MYC and facilitates cellular reprogramming

  Madhusudana Girija Sanal, et al., bioRxiv - Cell Biology 2023

  Quote: ... The membrane was washed with TBST buffer and incubated with a 1:5000 dilution of horseradish peroxidase-conjugated anti-rabbit (Santa Cruz, CA, Cat# SC-2004)/anti-mouse antibodies (Santa Cruz ...
• Aurora A mediated new phosphorylation of RAD51 is observed in Nuclear Speckles

  Mohamad Alaouid, et al., bioRxiv - Cell Biology 2023

  Quote: ... 5 µM Pladienolide B (SC-391691, Santa Cruz Biotechnology) for 4 hours ...
• Two RNA-binding proteins mediate the sorting of miR223 from mitochondria into exosomes

  Liang Ma, Jasleen Singh, Randy Schekman, bioRxiv - Cell Biology 2023

  Quote: ... YBAP1 (Santa Cruz Biotechnology, sc-271200).
• Scavenger receptor class B type I is required for efficient glucose uptake and metabolic homeostasis in adipocytes

  Darcy A. Knaack, et al., bioRxiv - Biochemistry 2023

  Quote: ... PE-GLUT4 antibody was purchased from Santa Cruz Biotechnology (Dallas ...
• Phosphorylation of a Cleaved Tau Proteoform at a Single Residue Inhibits Binding to the E3 Ubiquitin Ligase, CHIP

  Cory M. Nadel, et al., bioRxiv - Biochemistry 2023

  Quote: ... an aliquot (30 μL) of Protein A/G PLUS-agarose (Santa Cruz Biotech) per condition was collected and washed twice in ice cold IP lysis buffer ...
• Heparan sulfate modifications of betaglycan promote TIMP3-dependent ectodomain shedding to fine-tune TGF-β signaling

  Alex S Choi, et al., bioRxiv - Biochemistry 2023

  Quote: ... Actin antibody (C-2) (catalog sc-8432) was obtained from Santa Cruz Biotech (Santa Cruz ...
• SCF^FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation

  Ben Jin, et al., bioRxiv - Biochemistry 2023

  Quote: Human IL-17RA siRNA (#sc-40037) and nontargeting control RNA (#sc-37007) were obtained from Santa Cruz Biotechnology ...
• TOM20 Gates PINK1 Activity And Mediates Its Tethering Of The TOM And TIM23 Translocases Upon Mitochondrial Stress

  Mohamed A. Eldeeb, et al., bioRxiv - Biochemistry 2023

  Quote: ... The coverslips were fixed and stained for endogenous Tom20 (Santa Cruz Biotechnology, sc-17764) and exogenous HA (Biolegend ...
• SCF^FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation

  Ben Jin, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-IL-17RA (Santa Cruz Biotechnology ...
• SCF^FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation

  Ben Jin, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-HA (Santa Cruz Biotechnology ...
• SCF^FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation

  Ben Jin, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-p-ERK1/2 (Santa Cruz Biotechnology ...
• SCF^FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation

  Ben Jin, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-Akt (Santa Cruz Biotechnology ...
• SCF^FBXW11 complex targets interleukin-17 receptor A for ubiquitin-proteasome-mediated degradation

  Ben Jin, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-c-Myc (Santa Cruz Biotechnology ...
• Integrin β4 promotes DNA damage-related drug resistance in triple-negative breast cancer via TNFAIP2/IQGAP1/RAC1

  Huan Fang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and then incubated with Protein A/G plusagarose beads (sc-2003; Santa Cruz Biotechnology For the GST pull down assay ...
• Proximity Analysis of Native Proteomes Reveals Interactomes Predictive of Phenotypic Modifiers of Autism and Related Neurodevelopmental Conditions

  Yudong Gao, et al., bioRxiv - Neuroscience 2023

  Quote: ... the membrane was probed for Myc-epitope (Santa Cruz #sc-40, 1:250) and GAPDH (Abcam #ab9485 or Cell Signaling #2118 ...
• Simultaneous targeting of AMPK and mTOR is a novel therapeutic strategy against prostate cancer

  Gangyin Zhao, et al., bioRxiv - Cancer Biology 2023

  Quote: ... p-ACC (Santa Cruz Bio: sc-271965), and ACC (Cell Signaling ...
• Lineage-specific intolerance to oncogenic drivers restricts histological transformation

  Eric E. Gardner, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Cyp2f2-A488 (Santa Cruz; #sc-374540; 1:500), anti-BrdU (eBioscience #14-5071-82 ...
• RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry

  Jonathan Perr, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-hnRNP-U (Santa Cruz Biotechnology, sc-32315 AF647), anti-DDX21 (Novus Biologicals ...
• Weak neuronal glycolysis sustains cognition and organismal fitness

  Daniel Jimenez-Blasco, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-NDUFS1 (1/500) (sc-50132; Santa Cruz Biotechnology), anti-NDUFA9 (1/1,000 ...
• Regulation of Oocyte Meiotic Maturation: Unraveling the Interplay between PKA Inhibition and Cdk1 Activation

  Martina Santoni, et al., bioRxiv - Cell Biology 2023

  Quote: ... Karyopherin (1:2000, goat, Santa Cruz sc-1863), CPEB1 (1:2000 ...
• Regulation of Oocyte Meiotic Maturation: Unraveling the Interplay between PKA Inhibition and Cdk1 Activation

  Martina Santoni, et al., bioRxiv - Cell Biology 2023

  Quote: ... Erk1/2 (1:2000 each, rabbit, Santa Cruz Biotechnology C-16 and C-14), pT320-pp1 (1:1000 for the detection of the endogenous protein ...
• Weak neuronal glycolysis sustains cognition and organismal fitness

  Daniel Jimenez-Blasco, et al., bioRxiv - Cell Biology 2023

  Quote: ... a direct electrotransfer was performed followed by immunoblotting against mitochondrial complex I antibodies NDUFS1 (1/500) (sc-50132; Santa Cruz Biotechnology), NDUFA9 (1/1,000 ...
• Weak neuronal glycolysis sustains cognition and organismal fitness

  Daniel Jimenez-Blasco, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1/500 anti-Rock2 (sc398519; Santa Cruz Biotechnology) in in 0.2 % Triton X-100 (Sigma-Aldrich ...
• Exposure to titanium dioxide nanoparticles accelerates abnormal fat deposition through the mediation of the ROS via the AGE-RAGE signaling pathway in mice

  Jingyi Liu, et al., bioRxiv - Cell Biology 2023

  Quote: ... LPL (Santa Cruz, sc-32382), ATGL (Cell Signaling ...
• α-catenin mechanosensitivity as a route to cytokinesis failure through sequestration of LZTS2

  Yuou Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-GAPDH (Cat# sc-25778, Santa Cruz, now discontinued), polyclonal rabbit anti-GFP (A11122 ...
• Cdc42 couples septin recruitment to the axial landmark assembly via Axl2 in budding yeast

  Pil Jung Kang, et al., bioRxiv - Cell Biology 2023

  Quote: ... rabbit anti-GST antibody (Santa Cruz Biotechnology, cat#sc-459, Santa Cruz, CA; used at a dilution of 1:500), and rabbit monoclonal anti-calmodulin binding protein (Upstate Cell Signaling Solutions ...
• A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator

  Anniek Zaalberg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... HSP90 (sc-12119, Santa Cruz Biotechnologies, 1:1000) diluted in 4% BSA/PBS-T ...
• A genome-wide CRISPR screen in human prostate cancer cells reveals drivers of macrophage-mediated cell killing and positions AR as a tumor-intrinsic immunomodulator

  Anniek Zaalberg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... in SDS-PAGE 1X Running buffer (25 mM Tris, 0.25 M glycine, 0.1% SDS) and transferred on nitrocellulose membranes (Santa Cruz Biotechnology) in cold 1X Transfer buffer (24 mM Tris ...
• Inhibition of OCT4 Binding at the MYCN Locus Induces Neuroblastoma Cell Death Accompanied by Downregulation of Transcripts with High-Open Reading Frame Dominance

  Kazuma Nakatani, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-caspase-2 (1:1000 dilution; sc-5292, Santa Cruz Biotechnology, Dallas, TX), anti-caspase 3 (1:1000 dilution ...
• MEK1/2-Targeting PROTACs Promote the Collateral Degradation of CRAF in KRAS Mutant Cells

  Carlos Herrera-Montávez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... lysates were added to 20 μl RAF1 or MEK1 agarose conjugate (Santa Cruz) or normal mouse IgG agarose conjugate and incubated overnight ...
• Faulty Metabolism: A Potential Instigator of an Aggressive Phenotype in Cdk5-dependent Medullary Thyroid Carcinoma

  Priyanka Gupta, et al., bioRxiv - Cancer Biology 2023

  Quote: ... H1.5 (Santa Cruz sc-247158; RRID:AB_10847577; 1:1000), P-S988 RBL1 (Bibb Lab ...
• Intratumoral administration of recombinant murine interleukin-12 prevents tumor progression and bone invasion

  Shun Kasahara, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Immunohistochemical staining was performed as follows: the primary antibodies used included anti-cathepsin K (sc-48353, 1:200 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), anti-mouse CD3 (ab16669 ...
• ABCB1 overexpression through locus amplification represents an actionable target to combat paclitaxel resistance in pancreatic cancer cells

  Cecilia Bergonzini, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and mouse-anti-human β-actin (sc-47778; Santa Cruz Biotechnology ...
• ABCB1 overexpression through locus amplification represents an actionable target to combat paclitaxel resistance in pancreatic cancer cells

  Cecilia Bergonzini, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and mouse-anti-human β-actin (sc-47778; Santa Cruz Biotechnology, Santa Cruz, CA, USA; dilution 1:1000) antibodies ...
• Single-cell RNA sequencing reveals microenvironment context-specific routes for epithelial-mesenchymal transition in pancreas cancer cells

  Brooke A. Brown, Matthew J. Lazzara, bioRxiv - Cancer Biology 2023

  Quote: Antibodies against vimentin (Santa Cruz Biotechnology, sc-373717), E-cadherin (clone ECCD2 ...
• Single-cell RNA sequencing reveals microenvironment context-specific routes for epithelial-mesenchymal transition in pancreas cancer cells

  Brooke A. Brown, Matthew J. Lazzara, bioRxiv - Cancer Biology 2023

  Quote: ... and GAPDH (Santa Cruz Biotechnology, sc-32233) were used ...
• Targeting Ovarian Cancer Stem Cells by Dual Inhibition of the Long Noncoding RNA HOTAIR and Lysine Methyltransferase EZH2

  Weini Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Staining with p65 antibody (1:500, Santa Cruz) was followed by Alexa fluor-568 anti-mouse antibody (1:1000 ...
• Penile cavernous sinusoids are Prox1-positive hybrid vessels

  Sarah Schnabellehner, et al., bioRxiv - Cell Biology 2023

  Quote: ... rat anti-mouse EMCN (Santa Cruz Biotechnology, sc-65495; 1:200), rat anti-mouse PLVAP (BD Biosciences ...
• Tumor suppressor let-7 acts as a key regulator for maintaining pluripotency gene expression in Muse cells

  Gen Li, Shohei Wakao, Masaaki Kitada, Mari Dezawa, bioRxiv - Cell Biology 2023

  Quote: ... NRAS (1:200, Santa Cruz Biotechnology), p-AKT (T308 ...
• FOXK2 amplification and overexpression promotes breast cancer development and chemoresistance

  Yang Yu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... MO) and mouse IgG (sc-2025) were from Santa Cruz Biotechnology (Dallas ...
• Epidermal Growth Factor potentiates EGFR(Y992/1173)-mediated therapeutic response of triple negative breast cancer cells to cold atmospheric plasma-activated medium

  Peiyu Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: ... β-actin (Cat# sc-8432, Santa Cruz, USA), D9D5 K48-linkage polyubiquitin (Cat# 8081 ...
• Epidermal Growth Factor potentiates EGFR(Y992/1173)-mediated therapeutic response of triple negative breast cancer cells to cold atmospheric plasma-activated medium

  Peiyu Wang, et al., bioRxiv - Cancer Biology 2023

  Quote: Primary antibodies: EGFR (Cat# sc-120, Santa Cruz, USA), Phospho-EGFR (Tyr992 ...