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• Mapping host-microbe transcriptional interactions by dual perturb-seq

  Simon Butterworth, et al., bioRxiv - Molecular Biology 2023

  Quote: ... gondii (Santa Cruz) overnight at 4 °C ...
• Mapping host-microbe transcriptional interactions by dual perturb-seq

  Simon Butterworth, et al., bioRxiv - Molecular Biology 2023

  Quote: ... gondii (Santa Cruz) or 1:10,000 mouse anti-SAG1 (Laboratory of John Boothroyd ...
• Integrated multi-omics approach reveals the role of SPEG in skeletal muscle biology including its relationship with myospryn complex

  Qifei Li, et al., bioRxiv - Molecular Biology 2023

  Quote: ... mouse anti-RyR1 (sc-376507, 1:800 dilution, Santa Cruz Biotechnology), rabbit anti-CMYA5 (1:800 dilution ...
• Integrated multi-omics approach reveals the role of SPEG in skeletal muscle biology including its relationship with myospryn complex

  Qifei Li, et al., bioRxiv - Molecular Biology 2023

  Quote: ... mouse anti-FSD2 (sc-393072, 1:1000 dilution, Santa Cruz Biotechnology), and custom rabbit-anti-RyR1-pS2902 (YenZym ...
• Pathological Tau transmission initiated by binding lymphocyte-activation gene 3

  Chan Chen, et al., bioRxiv - Neuroscience 2023

  Quote: ... and mouse IgG (Santa Cruz, Dallas, TX, USA) were used as negative controls ...
• Boosting N-terminally anchored yeast surface display via structural insights into S. cerevisiae Pir proteins

  Tea Martinić Cezar, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Myc-tagged Ykl162c-a was probed with anti-c-Myc (1:1,000 dilution; 9E10, Santa Cruz Biotechnology, Dallas, United States) as a primary and mouse IgG κ-binding protein horseradish peroxidase (1:50,000 dilution ...
• Early and late chaperone intervention therapy boosts XBP1s and ADAM10, restores proteostasis, and rescues learning in Alzheimer’s Disease mice

  Jennifer M. Hafycz, et al., bioRxiv - Neuroscience 2023

  Quote: ... β-Actin (1:2000, Santa Cruz sc-47778); GluR1 (1:500 ...
• Reduction of retinal ganglion cell death in mouse models of familial dysautonomia using AAV-mediated gene therapy and splicing modulators

  Anastasia Schultz, et al., bioRxiv - Neuroscience 2023

  Quote: ... an anti-Brn3 antibody (1:200, Santa Cruz, #sc-6026), and an anti-ELP1(IKAP ...
• Transplanted Autologous Neural Stem Cells Show Promise in Restoring Motor Function in Monkey Spinal Cord Injury

  Razieh Jaberi, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-SOX2 (1:200, Santa Cruz, Sc-20088, the USA), The next day ...
• Transplanted Autologous Neural Stem Cells Show Promise in Restoring Motor Function in Monkey Spinal Cord Injury

  Razieh Jaberi, et al., bioRxiv - Neuroscience 2023

  Quote: ... cells were collected enzymatically (trypsin) and then staining was performed with selective antibodies as following: anti-SOX2 (1:200, Santa Cruz, Sc-20088, the USA), and anti-GFAP (1:400 ...
• The Prolonged Terminal Phase of Human Life Induces Survival Response in the Skin Transcriptome

  Ahmed S Abouhashem, et al., bioRxiv - Genomics 2023

  Quote: ... blocked with 10% NGS and incubated with primary antibody against AKT (cat#9272, 1:200 dilution) and COL1A2 (cat#sc-393573. Santa Cruz. 1:200) followed by appropriate fluorescence conjugated secondary antibodies (Alexa 568-tagged for AKT ...
• FOXM1 expression reverts aging chromatin profiles through repression of the senescence-associated pioneer factor AP-1

  Fábio J. Ferreira, et al., bioRxiv - Genetics 2023

  Quote: ... anti-p21 (F-5, sc-6246, Santa Cruz, 1:800) and anti p16INK4a (ab7962 ...
• N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules

  Pavel Kudrin, et al., bioRxiv - Molecular Biology 2023

  Quote: RNA was hydrolyzed to ribonucleosides by 20 U benzonase (Santa Cruz Biotech) and 0.2 U nuclease P1 (Sigma-Aldrich ...
• PKCδ regulates chromatin remodeling and DNA repair through SIRT6

  Trisiani Affandi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... RRID:AB_2152940), SIRT6 (Cat # sc-517556, RRID:AB_2915920), and Mre11 (Cat # sc-135992, RRID:AB_2145244) were purchased from Santa Cruz Biotechnology (Dallas ...
• Preclinical Screening of Splice-Switching Antisense Oligonucleotides in PDAC Organoids

  Ledong Wan, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... and mouse anti-Vinculin (1:1000, Santa Cruz Biotechnology) antibodies were used with IRDye 800CW secondary antibodies (LI-COR ...
• Regulation of lipolysis by 14-3-3 proteins on human adipocyte lipid droplets

  Qin Yang, et al., bioRxiv - Cell Biology 2023

  Quote: ... YHWAB (Santa Cruz Biotechnology, sc-25276), YWHAE (Cell Signaling Technology ...
• Complement C3 reduces apoptosis in human cardiomyocytes

  Zhou Fang, et al., bioRxiv - Cell Biology 2023

  Quote: ... Rabbit anti-caspase 3 polyclonal Ab was obtained from Santa Cruz Biotechnology (Dallas ...
• The E3 ubiquitin ligase Itch regulates death receptor and cholesterol trafficking to affect TRAIL-mediated apoptosis

  James Holloway, et al., bioRxiv - Cell Biology 2023

  Quote: ... and GAPDH (mouse, Cat# sc-47724, Santa Cruz). The following PE-conjugated antibodies were used in cell surface expression experiments ...
• Somatic chromosome pairing has a determinant impact on 3D chromatin organization

  Marta Puerto, et al., bioRxiv - Genomics 2023

  Quote: ... and αPc (Santa Cruz Biotech, Sc-25762).
• CHAF1A promotes the translesion DNA synthesis pathway in response to DNA replication stress

  Bing Wen, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-GFP-tag (sc-9996,, Santa Cruz Biotechnology; 1:1000), rabbit polyclonal anti-HA-tag (51064-2-AP ...
• Mitochondrial CISD1/Cisd accumulation blocks mitophagy and genetic or pharmacological inhibition rescues neurodegenerative phenotypes in Pink1/parkin models

  Aitor Martinez, et al., bioRxiv - Neuroscience 2023

  Quote: ... TOM20 (clone F-10, Santa Cruz, sc-17764, 1:1000), HA (Cell Signaling ...
• Shrew evolution in hybrid zones: a meiotic undecavalent as an exclusive chromosomal supermultivalent and its weak effect on fertility

  Sergey Matveevsky, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... we used the following corresponding secondary antibodies diluted in PBS: FITC-conjugated bovine anti-rabbit IgG (1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-rabbit Alexa Fluor 488 (1:400 ...
• Alphavirus-induced transcriptional and translational shutoffs play major roles in blocking the formation of stress granules

  Oksana Palchevska, et al., bioRxiv - Microbiology 2023

  Quote: ... eIF3b (sc-16377, Santa Cruz Biotechnology, Inc.) and TIAR (8509 ...
• ISWI1 complex proteins facilitate developmental genome editing in Paramecium

  Aditi Singh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Cells were stained overnight at 4 °C with a primary anti-HA antibody (sc-7392, Santa Cruz) followed by washing and secondary anti-mouse Alexa-594 conjugated antibody (BLD-405326 ...
• A Th17 cell-intrinsic glutathione/mitochondrial-IL-22 axis protects against intestinal inflammation

  Lynn Bonetti, et al., bioRxiv - Immunology 2023

  Quote: ... Membranes were washed and secondary mouse anti-rabbit IgG-HRP (Santa Cruz Biotechnology), which was diluted 1:5000 in PBS-T with 5% milk ...
• Unconventional Activation of IRE1 Enhances TH17 Responses and Promotes Neutrophilic Airway Inflammation

  Dandan Wu, et al., bioRxiv - Immunology 2023

  Quote: ... Immunoblot antibodies, anti-XBP1 (M186, sc-7160) and anti-JAK2 (C-10, sc- 390539) were purchased from Santa Cruz Biotechnology ...
• Mmp17-deficient mice exhibit heightened goblet cell effector expression in the colon and increased resistance to chronic Trichuris muris infection

  Pia M. Vornewald, et al., bioRxiv - Immunology 2023

  Quote: ... anti-MUC2 (1:200, Santa Cruz Biotechnology, sc-15334), anti-CLCA1 (1:200 ...
• Inherently reduced expression of ASC restricts caspase-1 processing in hepatocytes and promotes Plasmodium infection

  Camila Marques-da-Silva, et al., bioRxiv - Immunology 2023

  Quote: ... anti-ASC (F-9, Santa Cruz), anti-IL-1β (polyclonal ...
• Inherently reduced expression of ASC restricts caspase-1 processing in hepatocytes and promotes Plasmodium infection

  Camila Marques-da-Silva, et al., bioRxiv - Immunology 2023

  Quote: ... or mASC (F-9, Santa Cruz) at 40C overnight ...
• Post-exposure intranasal IFNα suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons

  Matthew D. Cain, et al., bioRxiv - Immunology 2023

  Quote: ... 5% normal donkey serum (Santa Cruz Biotechnology) with 0.1% Triton X-100 (Sigma-Aldrich) ...
• Macrophages sense ECM mechanics and growth factor availability through cytoskeletal remodeling to regulate their tissue repair program

  Matthew L. Meizlish, et al., bioRxiv - Immunology 2023

  Quote: ... and mouse anti-GAPDH (Santa Cruz, sc-32233). Membranes were washed three times with TBST ...
• DLL4-Notch3-WNT5B axis is a novel mediator of bi-directional pro-metastatic crosstalk between melanoma and lymphatic endothelial cells

  Sanni Alve, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The chromatin was precipitated using antibodies against Notch3 (M-134 Santa Cruz Biotechnology or 8G5 Cell Signaling) or normal goat or rat IgG antibody (Santa Cruz Biotechnology) ...
• DLL4-Notch3-WNT5B axis is a novel mediator of bi-directional pro-metastatic crosstalk between melanoma and lymphatic endothelial cells

  Sanni Alve, et al., bioRxiv - Cancer Biology 2023

  Quote: ... or normal goat or rat IgG antibody (Santa Cruz Biotechnology). DNA was purified using the PCR Purification kit (Macherey-Nagel ...
• Phytocystatin 6 is a context-dependent, tight-binding inhibitor of Arabidopsis thaliana legumain isoform β

  Naiá P. Santos, et al., bioRxiv - Biochemistry 2023

  Quote: ... membranes were washed with TBST and incubated with goat anti-rabbit-IgG coupled to horseradish peroxidase (Santa Cruz Biotechnology, Dallas, USA) in a 1:12000 dilution in TBST for 1 hour ...
• Shifting the selectivity of pyrido[2,3-d]pyrimidin-7(8H)-one inhibitors towards the salt-inducible kinase (SIK) subfamily

  Marcel Rak, et al., bioRxiv - Biochemistry 2023

  Quote: ... mouse monoclonal anti-PLK1 (Santa Cruz Biotechnology).
• Base Editor Scanning Reveals Activating Mutations of DNMT3A

  Emma M. Garcia, et al., bioRxiv - Biochemistry 2023

  Quote: ... K562s were transduced with the appropriate lentivirus by spinfection (1,800g, 90 min, 37 °C) with 12 μg ml−1 polybrene (Santa Cruz Biotechnology). 2 μg ml−1 puromycin (Gibco ...
• Local production of reactive oxygen species drives vincristine-induced axon degeneration

  Jorge Gómez-Deza, et al., bioRxiv - Neuroscience 2023

  Quote: ... The following antibodies were used at the concentrations stated: anti-DRP1 (Santa Cruz Biotechnology ...
• GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

  Liang Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse anti-myc (1:100, sc-40, Santa Cruz Biotechnology), rabbit anti-COXIV (1:100 ...
• GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation

  Liang Wang, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse anti-TOM20 (1:1000, sc-17764, Santa Cruz Biotechnology), rabbit anti-TOM40 (1:1000 ...
• A role for Vps13-mediated lipid transfer at the ER-endosome contact site in ESCRT-mediated sorting

  Sho W. Suzuki, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse monoclonal anti-GFP (B-2; Santa Cruz, Sc-9996), rabbit polyclonal anti-G6PDH (Sigma-Aldrich ...
• Olfactory cortex output pathways exhibit robust differences in their ability to drive learned aversion

  Inês Vieira, Dmitri Bryzgalov, bioRxiv - Neuroscience 2023

  Quote: ... The following antibodies were used at the indicated dilutions: rabbit anti-cfos 1:500 (Santa Cruz sc-7270), and chicken anti-GFP 1:1000 (Abcam ab13970) ...
• Nucleolar stress caused by arginine-rich peptides triggers a ribosomopathy and accelerates ageing in mice

  Oleksandra Sirozh, et al., bioRxiv - Molecular Biology 2023

  Quote: ... UBF (Santa Cruz, sc-13125) was performed using standard procedures ...
• Different Purkinje cell pathologies cause specific patterns of progressive ataxia in mice

  Dick Jaarsma, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-FOXP2 (Santa Cruz Biotechnology ...
• Structural insights into complex I deficiency and assembly from the disease-related ndufs4^-/- mouse

  Zhan Yin, et al., bioRxiv - Biochemistry 2023

  Quote: ... and its inhibitor sensitivity checked by addition of 2 μM atpenin A5 (Santa Cruz Biotechnology, USA). Flavin-site activity measurements were carried out in the presence of 15 μg mL-1 alamethicin and 2 μM piericidin A as follows ...
• LPCAT2 Regulates CD14 Expression During Macrophage Inflammatory Response to E. coli O111:B4

  Victory Ibigo Poloamina, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and protein ladders were obtained from Santa Cruz Biotechnology ...
• Deficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice

  Adriana C. Norris, et al., bioRxiv - Molecular Biology 2023

  Quote: ... DGAT2 (sc-66859, Santa Cruz Biotech), phospho-AMPKαT172 (#2535 ...
• Development of an optimized, non-stem cell line for intranasal delivery of therapeutic cargo to the central nervous system

  Ali El-Ayoubi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Immunofluorescence was performed using the YB-1 antibody from Santa Cruz (#R0409, Santa Cruz Biotechnology, Heidelberg, Germany). As a secondary antibody anti-Mouse IgG Alexa Fluor™ Plus 680 (Invitrogen ...
• In vivo imaging in transgenic songbirds reveals superdiffusive neuron migration in the adult brain

  Naomi R. Shvedov, et al., bioRxiv - Neuroscience 2023

  Quote: ... migratory neurons (Santa Cruz Biotechnology, sc-271390 (E-6), lot ...
• Nischarin negatively impacts ShcD-mediated tumor cell migration

  Rayan A. Hago, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-GFP (sc9996-Santa Cruz, USA), anti-phospho Erk1/2 (cst4370S-Cell Signaling ...
• CCT3 drives Sorafenib resistance by inhibiting TFRC-mediated iron uptake in HCC

  Huihui Zhu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... protein A/G (Santa Cruz Biotechnology, sc-2003), FITC-transferrin (Rockland Immunochemicals ...