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• In situ architecture of Opa1-dependent mitochondrial cristae remodeling

  Michelle Y. Fry, et al., bioRxiv - Biophysics 2023

  Quote: ... Cells were washed in PBS and coated with UltraCruz® Aqueous Mounting Medium with DAPI (Santa Cruz) prior to imaging ...
• Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants

  Ann-Kathrin Reuschl, et al., bioRxiv - Microbiology 2023

  Quote: ... dsRNA and nucleocapsid detection were performed by primary incubation with rabbit-anti-IRF3 antibody (sc-33641, Santa Cruz), rabbit-anti-STAT-1 (D1K9Y ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... supernatant from immunoprecipitation experiments was incubated with primary antibody overnight at 4°C followed by incubation with 20 µL of Protein A/G Plus-agarose beads (Santa Cruz Biotechnology, Inc.) for 2 h at 4°C the following day ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Myosin ID (1:500; H-1; sc-515292; Santa Cruz Biotechnology, Inc). For fluorescence intensity measurements ...
• Simultaneous detection of membrane contact dynamics and associated Ca²⁺ signals by reversible chemogenetic reporters

  Paloma García Casas, et al., bioRxiv - Cell Biology 2023

  Quote: ... Cells were incubated overnight at 4°C with primary antibodies (α-TOM20 Santa Cruz # sc-11415; α-STIM1 Merck WH0006786M1) diluted (1:200 ...
• TLR2 Regulates Hair Follicle Cycle and Regeneration via BMP Signaling

  Luyang Xiong, et al., bioRxiv - Immunology 2023

  Quote: ... myeloperoxidase (Santa Cruz Biotechnology, sc-390109), GFP (Thermofisher Scientific ...
• TLR2 Regulates Hair Follicle Cycle and Regeneration via BMP Signaling

  Luyang Xiong, et al., bioRxiv - Immunology 2023

  Quote: The following antibodies or reagents were used: Ker17 (Santa Cruz Biotechnology, sc-393002), Ker15 (ABclonal ...
• PDGFRα/β heterodimer activation negatively affects downstream ERK1/2 signaling and cellular proliferation

  Maria B. Campaña, et al., bioRxiv - Cell Biology 2023

  Quote: ... Myosin ID (1:500; H-1; sc-515292; Santa Cruz Biotechnology, Inc); GAPDH (1:50000 ...
• Mechanisms by which barrier-to-autointegration factor regulates dynamics of nucleocytoplasmic leakage and membrane repair following nuclear envelope rupture

  Charles T. Halfmann, et al., bioRxiv - Cell Biology 2023

  Quote: ... along with polybrene (2.5 µg/mL; Santa Cruz Biotechnology), and incubated at 37°C for 24 hr ...
• Mechanisms by which barrier-to-autointegration factor regulates dynamics of nucleocytoplasmic leakage and membrane repair following nuclear envelope rupture

  Charles T. Halfmann, et al., bioRxiv - Cell Biology 2023

  Quote: ... Mouse monoclonal anti-tubulin was used as a loading control (sc-32293; Santa Cruz Biotechnology) at 1:2000 dilution ...
• Brg1 regulates fibroblast-myofibroblast transition to promote renal fibrosis

  Xiaoyan Wu, et al., bioRxiv - Pharmacology and Toxicology 2023

  Quote: ... Aliquots of lysates containing 100 μg of protein were used for each immunoprecipitation reaction with indicated antibodies followed by adsorption to protein A/G PLUS-agarose beads (Cat#: sc-2003, Santa Cruz Biotechnology). DNA-protein cross-link was reversed by heating the samples to 65 °C overnight ...
• PAK3 downregulation induces cognitive impairment following cranial irradiation

  Haksoo Lee, et al., bioRxiv - Neuroscience 2023

  Quote: ... membranes were probed with specific primary antibodies and peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology). The samples were subsequently analyzed using an ECL detection system (Roche Applied Science ...
• Exoribonuclease RNase R protects Antarctic Pseudomonas syringae Lz4W from DNA damage and oxidative stress

  Pragya Mittal, et al., bioRxiv - Microbiology 2023

  Quote: ... Antibodies were purchased from Santa Cruz Biotechnology.
• Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing is associated with therapeutic response to splicing inhibitor

  Carolyn Jablonowski, et al., bioRxiv - Cancer Biology 2023

  Quote: ... JMJD6 (Santa Cruz biotechnology ...
• ERBB signalling contributes to immune evasion in KRAS-driven lung adenocarcinoma

  Sarah Laing, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Vinculin (Santa Cruz 25336, 1:1000). Secondary horseradish peroxidase conjugated antibodies were added at a concentration of 1:7000 in 5% milk in TBST (HRP anti-mouse NA931V ...
• Extrachromosomal Telomeres Derived from Excessive Strand Displacements

  Junyeop Lee, et al., bioRxiv - Biochemistry 2023

  Quote: ... anti-ATRX (Santa Cruz, sc-15408), anti-hMRE-11 (Calbiochem ...
• ER-to-lysosome Ca²⁺ refilling followed by K⁺ efflux-coupled store-operated Ca²⁺ entry in inflammasome activation and metabolic inflammation

  Hyereen Kang, et al., bioRxiv - Immunology 2023

  Quote: ... HSP 90 (Santa Cruz sc13119, 1: 1,000) and β-actin (Santa Cruz sc47778 ...
• Nucleosome reorganisation in breast cancer tissues

  Divya R. Jacob, et al., bioRxiv - Genomics 2023

  Quote: ... Primary antibodies used were rabbit anti-ERα (1:1000, sc- 543, Santa Cruz, TX, USA), mouse α-tubulin (1:10,000 ...
• Ablation of Sam50 is associated with fragmentation and alterations in metabolism in murine and human myotubes

  Bryanna Shao, et al., bioRxiv - Physiology 2023

  Quote: ... myotubes were infected with 1 µg CRISPR/Cas9 (Santa Cruz Sam50 CRISPR Plasmid; sc-427129) to achieve Sam50 deletion ...
• Parvalbumin Interneuron Activity Underlies Vulnerability to Post-Traumatic Stress Disorder in Autism

  A. Shaam Al Abed, et al., bioRxiv - Neuroscience 2023

  Quote: ... Tissues were then stained using the primary antibodies overnight: mouse anti-c-Fos (1:1000; Santa Cruz), mouse anti-PV (Swan) ...
• WNK1-dependent water influx is required for CD4⁺ T cell activation and T cell-dependent antibody responses

  Joshua Biggs O’May, et al., bioRxiv - Immunology 2023

  Quote: ... NP20-BSA (bovine serum albumin) (5μg/ml, Santa Cruz Biotechnology) was used to coat 96-well Maxisorp Immunoplates (Nalge Nunc International Corporation ...
• Identification of a P62-TIF-IA axis that drives nucleolar fusion and the senescence associated secretory phenotype

  Hazel C Thoms, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Rrn3 (Mouse, 1:500, Santa Cruz sc-390464); p62 (Mouse ...
• Intrinsic disorder specifies discreet functions of nucleoplasmin-like histone chaperones

  Courtney M. Gauthier, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Antibodies to Myc (9E10, Santa Cruz) and Fpr3 (a kind gift of Dr ...
• Identification and characterization of interactions between Influenza A Virus NS1 protein and the human ubiquitin proteasome system

  Laurie-Anne Lamotte, et al., bioRxiv - Molecular Biology 2023

  Quote: ... the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568, Santa Cruz Biotechnology), β-Tubulin Rabbit Polyclonal Antibody (10094-I-AP ...
• Identification and characterization of interactions between Influenza A Virus NS1 protein and the human ubiquitin proteasome system

  Laurie-Anne Lamotte, et al., bioRxiv - Molecular Biology 2023

  Quote: ... and β-Actin Mouse Monoclonal Antibody (sc-69879, Santa Cruz Biotechnology). Blots were incubated with the corresponding secondary antibody for 1 h at room temperature ...
• Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells

  Yana Blokhina, Abigail Buchwalter, bioRxiv - Molecular Biology 2023

  Quote: ... and stained with RPA194 (C-1) (Pol I subunit) mouse monoclonal antibody (Santa Cruz, sc-48385) in IF buffer for 1 hour at RT ...
• CCT3 drives Sorafenib resistance by inhibiting TFRC-mediated iron uptake in HCC

  Huihui Zhu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Ferritin antibody (Santa Cruz Biotechnology, sc-376594), anti- SLC7A11 antibody (Cell Signaling Technology ...
• Arrestin-3-assisted activation of JNK3 mediates dopaminergic behavioral and signaling plasticity in vivo

  Mohamed R. Ahmed, et al., bioRxiv - Neuroscience 2023

  Quote: ... JNK3-specific mouse (Santa Cruz Biotechnology) or rabbit anti-HA antibody (Cell Signaling ...
• Targeted brain-specific tauopathy compromises peripheral skeletal muscle integrity and function

  Bryan Alava, et al., bioRxiv - Neuroscience 2023

  Quote: ... Tau46 (Santa Cruz Biotechnology Inc. sc-32274; 1:1000), or AT8 primary antibody (Thermo Fisher Scientific MN1020 ...
• SPINAL V1 INHIBITORY INTERNEURON CLADES DIFFER IN BIRTHDATE, PROJECTIONS TO MOTONEURONS AND HETEROGENEITY

  Andrew E. Worthy, et al., bioRxiv - Neuroscience 2023

  Quote: ... Similar procedures were used to detect Foxp2 immunoreactivity (Santa Cruz) except that in this case the blot was probed only once ...
• SELENOT regulates endoplasmic reticulum calcium flux via SERCA2 and maintains dopaminergic DAT to protect against attention deficit hyperactivity disorder in mice

  Qing Guo, et al., bioRxiv - Neuroscience 2023

  Quote: ... AdA (sc-221213) from Santa Cruz Biotechnology ...
• GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology

  Sophia M. DiCesare, et al., bioRxiv - Neuroscience 2023

  Quote: ... blots were incubated for 1 h at RT with primary antibody [GSK3α (sc-5264, Santa Cruz), GSK3β (9315S ...
• GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology

  Sophia M. DiCesare, et al., bioRxiv - Neuroscience 2023

  Quote: ... or GAPDH (sc-47724, Santa Cruz)] diluted in 5% bovine serum albumin (BSA ...
• GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology

  Sophia M. DiCesare, et al., bioRxiv - Neuroscience 2023

  Quote: Cells for western blotting were rinsed with HBSS followed by lysis in buffer containing radioimmunoprecipitation buffer (RIPA, Santa Cruz), protease inhibitor (Pierce ...
• HELZ promotes R loop resolution to facilitate DNA double-strand break repair by homologous recombination

  Ramona Haji-Seyed-Javadi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... GAPDH (Santa Cruz tech # sc-47724; 1:1000 for western); 53BP1 (Bethyl # A300-273A ...
• HELZ promotes R loop resolution to facilitate DNA double-strand break repair by homologous recombination

  Ramona Haji-Seyed-Javadi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... RPA32 (Santa Cruz Tech # sc-14692; 1:400 for western); pRPA32 S4/8 (Bethyl # A700-009 ...
• Genome-wide ribosome profiling uncovers the role of iron in the control of protein translation

  Hanna Barlit, et al., bioRxiv - Genetics 2023

  Quote: ... and HRP-conjugated secondary anti-mouse antibody (Santa Cruz Biotechnology) were used to detect Pgk1 protein levels as a loading control.
• Nuclear NFκB Activity Balances Purine Metabolism in Cellular Senescence

  Sho Tabata, et al., bioRxiv - Systems Biology 2023

  Quote: ... HRP-linked anti-goat IgG (1:5000; Santa Cruz Biotechnology, Inc., sc-2020), and HRP-linked anti-rabbit IgG (1:5000 ...
• OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion

  Styliani Panagiotou, et al., bioRxiv - Cell Biology 2023

  Quote: ... and β-actin mouse monoclonal (clone C4, sc-47778, 1:300, Santa Cruz Biotechnology).
• OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion

  Styliani Panagiotou, et al., bioRxiv - Cell Biology 2023

  Quote: ... π-actin monoclonal (clone C4, sc-47778, Santa Cruz Biotechnology, host: mouse, 1:300), IP3R1 polyclonal (catalogue no ...
• Active Suppression of the Nigrostriatal Pathway during Optogenetic Stimulation Revealed by Simultaneous fPET/fMRI

  Sabrina Haas, et al., bioRxiv - Neuroscience 2023

  Quote: ... The slides were stained with the antibodies c-fos (SC-52, Santa Cruz Biotechnology, Dallas, TX, USA) and TH (Tyrosine Hydroxylase (#22941) ...
• SPINAL V1 INHIBITORY INTERNEURON CLADES DIFFER IN BIRTHDATE, PROJECTIONS TO MOTONEURONS AND HETEROGENEITY

  Andrew E. Worthy, et al., bioRxiv - Neuroscience 2023

  Quote: ... EGFP was amplified with chicken anti-GFP (Aves) and combined with goat anti-Foxp2 (Santa Cruz), rabbit anti-Otp (ThermoFisher ...
• SPINAL V1 INHIBITORY INTERNEURON CLADES DIFFER IN BIRTHDATE, PROJECTIONS TO MOTONEURONS AND HETEROGENEITY

  Andrew E. Worthy, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-Foxp2 (Santa Cruz), or goat anti-Sp8 (Santa Cruz) ...
• FeLIX is a restriction factor for mammalian retrovirus infection

  Didik Pramono, et al., bioRxiv - Microbiology 2023

  Quote: ... or HRP-conjugated donkey anti-goat IgG (Santa Cruz Biotechnology; 1:10000). The substrate was LumiGLO® Reagent (20× ...
• FeLIX is a restriction factor for mammalian retrovirus infection

  Didik Pramono, et al., bioRxiv - Microbiology 2023

  Quote: ... conjugated with protein G- agarose (Santa Cruz Biotechnology), and centrifuged at 15,400 × g for 2 min at 4 °C.
• Postnatal intestinal epithelial maturation by LSD1 controls the small intestinal immune cell composition independently from the microbiota

  Alberto Díez-Sánchez, et al., bioRxiv - Immunology 2023

  Quote: ... (MUC2, 1:200, Santa Cruz Biotechnology, sc-15334), (LYZ ...
• Identification of Host Restriction Factors Critical for Recombinant AAV Transduction of Polarized Human Airway Epithelium

  Siyuan Hao, et al., bioRxiv - Microbiology 2023

  Quote: ... were added to the cell culture at an MOI of 0.25 in the presence of polybrene (Santa Cruz, Dallas, Texas). At 24 hours post-transduction ...
• Chlamydial protease-like activating factor targets SLC7A11 for degradation to induce ferroptosis and facilitate dissemination

  Wentao Chen, et al., bioRxiv - Microbiology 2023

  Quote: ... fibroblast-specific protein 1 (FSP1; 1:500, Santa Cruz Biotechnology Cat# sc-377120), TfR1(1:500 ...
• Chlamydial protease-like activating factor targets SLC7A11 for degradation to induce ferroptosis and facilitate dissemination

  Wentao Chen, et al., bioRxiv - Microbiology 2023

  Quote: ... TfR1(1:500, Santa Cruz Biotechnology Cat# sc-32272), Vimentin (1:1000 ...
• Social Determinants modulate NK cell activity via obesity, LDL, and DUSP1 signaling

  Yvonne Baumer, et al., bioRxiv - Immunology 2023

  Quote: ... and galectin-8-AF790 (clone C-8, Santa Cruz Biotechnologies, USA)