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Citations for Miltenyi Biotec :
4651 - 4700 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Blood and isolation cocktail mix were placed in the MACSxpress Separator (Miltenyi Biotec) and allowed to separate for 15 minutes ...
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bioRxiv - Immunology 2022Quote: Neutrophils were isolated using the MACSxpress Whole Blood Neutrophil Isolation kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Breast tissue was enzymatically and mechanically dissociated as per the Human Tumor Dissociation Kit (Miltenyi Biotec) protocol ...
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bioRxiv - Immunology 2022Quote: ... The dissociated breast sample was then passed through a 100 µm MACS Smart Strainers (Miltenyi Biotec), topped up with RPMI 1640 10% FCS then centrifuged at 300 x g for 5 min ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified from human PBMCs by using human CD4 MicroBeads (Miltenyi Biotec). CD4+ CD25Lo CD127Hi Tconv cells and CD4+ CD25Hi CD127Lo Treg cells were sorted by flow cytometry and stimulated in 96-well cell-culture plates with three times the number of Dynabeads M-450 Tosylactivated beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were purified from CD4+ T cells from pooled spleens and lymph nodes of Cd226−/− 2D2 TCR Tg mice by using mouse CD4 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany), followed by flow cytometric sorting for CD25-CD44LoCD62LHiCD4+ cells ...
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bioRxiv - Cell Biology 2022Quote: ... incubated for 15 min at room temperature and applied onto the dead cell removal column (cat # 130-042-401, Miltenyi Biotec), which was attached to the MACS separator ...
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bioRxiv - Cell Biology 2022Quote: ... SVCs were labeled using the mouse Lineage Cell Depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cellular debris was removed by density gradient centrifugation using Debris Removal Solution (Miltenyi Biotec). Cells recovered were resuspended in PBS containing 0.5% FF-BSA.
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed from the digested PDX using a Dead Cell Removal Kit (130-090-101, Miltenyi Biotec). Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694 ...
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bioRxiv - Cancer Biology 2022Quote: ... Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was then transferred to a sterile gentleMACS C tube (Miltenyi Biotec, Cat. No. 130096334), resuspended in 3-5 mL of Miltenyi Tumor Dissociation Kit buffer (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... The suspension was then digested utilizing the gentleMACS Octo Dissociator (Miltenyi Biotec, Cat. No. 130-095-937) for 25-30 min using the “37C_h_TDK_3’’ program for tumor tissue ...
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bioRxiv - Cancer Biology 2022Quote: ... CD133-PE (PROM1) antibody (Miltenyi Biotec, 130-113-670, 1:50), and CD338-PE (ABCG2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and CD338-PE (ABCG2) antibody (Miltenyi Biotec, 130-105-010, 1:50) for 1 h on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl of cell suspension was incubated with CD117-APC (c-KIT) antibody (Miltenyi Biotec, 130-098-207, 1:50), CD133-PE (PROM1 ...
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bioRxiv - Developmental Biology 2022Quote: Optically cleared samples were imaged using an Ultramicroscope II Super Plan (Miltenyi Biotec, Bergisch Gladbach, Germany) equipped with 4x MI Plan Objective (NA 0.35 ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing NPCs was counted and Kupffer cells (KC) were removed using anti-F4/80 Ultrapure microbeads (Miltenyi Biotec) following manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2022Quote: Naïve mouse CD4+ T cells were isolated from spleens of Atg5+/+ and Atg5flox/flox mice using Naïve CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Cells (5 × 105 cells in one milliliter per well ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP [GG4-2C2.12.10] (1:5000; 130-091-833; Miltenyi Biotec), rabbit anti-EXOC1 antibody (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... Half of the cells were resuspended in binding buffer (cat# 130-090-101, Miltenyi Biotec) with dead cell removal beads ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were dissociated using TrypLE Express and enriched for CD34+ endothelial progenitor cells using the CD34 MicroBead Kit (Miltenyi Biotec). Purified cells resuspended in EGM-2 media (Lonza ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by passing through 70 μm Pre-Separation Filters (130-110-204, Miltenyi Biotec) to achieve single-cell suspension solution ...
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bioRxiv - Cell Biology 2022Quote: ... Labeled samples were passed onto MS columns (Miltneyi Biotec) on the OctoMACS separator (Miltenyi Biotec); the flow through was collected as the Lin- cell fraction and bound Lin+ cells were eluted from the column ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from magnetic-bead isolated cKit+ bone marrow (Miltenyi Biotec) using the Direct-zol RNA extraction kit (Zymo ...
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bioRxiv - Cancer Biology 2022Quote: ... or Annexin V-FITC Kit (Miltenyi Biotec). All data were processed and analyzed with FCS Express 7 Research Edition (De Novo Software ...
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bioRxiv - Cell Biology 2022Quote: ... ErbB2 (CD34) PE-Vio770 used for FACS sorting of HER2+ stable cells was obtained from Miltenyi Biotec (Germany).
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bioRxiv - Cell Biology 2022Quote: ... and filtered through 30 µm MACS SmartStrainer (Miltenyi Biotec, Cat#130-098-458). Nuclei were centrifuged (590xg ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Developmental Biology 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec). Cells were maintained at 37°C with 6% CO2.
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bioRxiv - Bioengineering 2022Quote: ... and the sample was physically dissociated using a GentleMACS dissociator (Miltenyi Biotec, #130-096-334) using the “skeletal muscle” setting ...
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bioRxiv - Bioengineering 2022Quote: ... through an LS column in a magnetic field (Miltenyi Biotec, #130-042-401) (Supplementary Table 1) ...
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bioRxiv - Bioengineering 2022Quote: The cell pellet was then incubated in a 4 °C fridge with rocking for 15 minutes in 100 µL of MACS buffer and 25 µL of lineage depletion microbeads from the Satellite Cell Isolation Kit (Miltenyi Biotec, #130-104-268) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... ≈1 gram of muscle tissues was dissected from the hindlimb muscles of a humanely euthanized mouse and placed into a GentleMACS dissociation tube (Miltenyi Biotec, #130-096-334). 7 mL of DMEM (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... The solution was passed through a 70 µm cell strainer (Miltenyi Biotec, #130-098-462) followed by a 40 µm cell strainer (Corning ...
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bioRxiv - Genomics 2022Quote: ... Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and subsequently isolation of CD1c+ cells by using the CD1c+ (BDCA1) isolation kit (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and 50ng/mL transforming growth factor β1 (TGFβ1; Miltenyi Biotec # 130-108-969) (MBM + 3GFs) ...
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bioRxiv - Cancer Biology 2022Quote: ... Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec, Bergisch Gladbach, Germany). Pairs of liver samples from the same experimental group were pooled and subjected to a second round of purification ...
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bioRxiv - Genomics 2022Quote: ... brain tissue was processed using a MACS Neural Tissue Dissociation Kit (T) (Miltenyi Biotec, Cat#: 130-093-231) and manually dissected according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... according to manufacturer’s instructions or with EBV pepmix (PepTivator EBV Consensus peptide pool (Miltenyi Biotec)) ...
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bioRxiv - Immunology 2022Quote: ... The RPMI medium was additionally supplemented with 200 ng/mL human Flt3 ligand (Miltenyi Biotec, 130-096-479) and 5 ng/mL murine GM-CSF (Peprotech ...
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bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 ng/mL ACTIVIN A (Miltenyi Biotec) and 1.5 μM CHIR99021 (Axon Medchem) ...
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bioRxiv - Immunology 2022Quote: ... and CD4+ or CD8+ T cells were isolated using CD4+ or CD8+ T cells isolation kits (Miltenyi Biotec) as described in the manufacture protocols ...