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Citations for Miltenyi Biotec :
4801 - 4850 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... human positive selection kit (Miltenyi Biotec) and total RNA was extracted from hCD45+ cells using NucleoSpin® RNA kit (Macherey-Nagel ...
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bioRxiv - Immunology 2022Quote: ... mouse” (Miltenyi Biotec Inc, Auburn, CA, USA) were used according to the protocol suggested by the manufacturer ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Immunology 2022Quote: ... samples were first positively selected for CD11c+ cells using Mouse UltraPure CD11c Microbeads (Miltenyi Biotec) prior to surface marker staining ...
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bioRxiv - Immunology 2022Quote: ... We obtained lung single cell suspensions with the Mouse Lung Dissociation Kit (Miltenyi Biotec). We stained the cells with an antibody solution to detect CD8+ T cells ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions obtained from spleens were processed with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec; Cat#: 130-104-075) and transferred via the tail vein into recipient mice.
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bioRxiv - Immunology 2022Quote: ... We isolated CD8+ T cells from spleens with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) and plated them on dishes coated with an antibodies for CD3 (2 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... brains were collected from P2-P5 pups and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628) on a gentleMACS Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2022Quote: Tumor and lymph node samples were thoroughly minced with surgical scissors and transferred to GentleMACs C Tubes (Miltenyi Biotec) containing 800 U/ml Collagenase IV (Worthington ...
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bioRxiv - Immunology 2022Quote: ... We isolated and purified tumor cells by Tumor Cell Isolation Kit (Miltenyi Biotec) and cultured until 4th generation ...
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bioRxiv - Immunology 2022Quote: ... GentleMACs C Tubes were then installed onto the GentleMACs Octo Dissociator (Miltenyi Biotec) and incubated for 20 min (lymph node ...
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bioRxiv - Immunology 2022Quote: ... Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec). For plasma cell differentiation ...
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bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: Dissected and trimmed tumors were washed in pre-cooled RNase-free H2O and transferred into MACS Tissue storage solution (Miltenyi Biotec, 130-100-008). Samples were shipped on ice overnight to Novogene ...
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bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Immunology 2022Quote: Monocytes were purified from PBMCs by CD14 positive magnetic selection (Miltenyi Biotec, Bergisch Gladbach, Germany) and plated at 2.106 cells/ml in RPMI 1640 medium containing 10% fetal calf serum (FCS) ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from the splenocytes using Pan T Cell Isolation Kit II (Miltenyi BioTec) according to the manufacturers protocol and 1×106 T cells were transferred to Thy1.1 mice by tail vein injection ...
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bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal macrophages were isolated from adult (8-10 weeks old) female mice using Peritoneum Macrophage Isolation Kit (130-110-434, Miltenyi Biotec). Isolated cells were cultured in complete RPMI+GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Total splenic B cells (CD19+) were purified using CD19 MicroBeads (130-121-301, Miltenyi Biotec, San Jose, CA), and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862 ...
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bioRxiv - Immunology 2022Quote: ... DCs were isolated from murine lymph nodes using magnetic beads according to the manufacturer's instructions (Miltenyi Biotec, Cat.No. 130-100-875). OT-1 T cells were isolated from peripheral blood of OT-1 mice and labeled with 0.5 μM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... and dissociated in gentle MACS M tubes using the gentleMACS Octo Dissociator (Miltenyi Biotec). Samples were transferred into Phasemaker tubes (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
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bioRxiv - Cancer Biology 2022Quote: ... Nonspecific binding was blocked by using 1% Fc block (Miltenyi Biotec). Cells were stained with HLA-A2 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in StemMACS™ iPS-Brew XF medium (Miltenyi Biotec) supplemented with 1% Pen-Strep (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
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bioRxiv - Cancer Biology 2022Quote: ... Fc receptors were blocked using Fcr Blocking Reagent (Miltenyi Biotec, 130-059-901) according to manufacter’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... After an additional 24h co-cultured cells were trypsinized and endothelial cells were purified using CD31 microbead kit (Miltenyi Biotec #130-091-935) according to manufacturer instructions.
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bioRxiv - Developmental Biology 2022Quote: ... a region spanning the dorsal pallial midline (see Figure 1A) was dissected on both hemispheres and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) on the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell viability and concentration were assessed using a MACSQuant flow cytometer (Miltenyi Biotec). Approximatively 20000 cells were used as input on the 10X Genomics Chromium Controller for a targeted cell recovery of 10000 cells per library ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Cell Biology 2022Quote: ... [26] using the B cell enrichment kit (Pan B Cell Isolation Kit, Miltenyi Biotec, EUA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 μl Enzyme R and 25 μl Enzyme A (Cat. No. 130-095-929 Miltenyi Biotec). Afterwards ...
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bioRxiv - Cancer Biology 2022Quote: Samples were processed immediately after surgery and temporarily stored for transport at 4°C in tissue storage solution (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously described ...
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bioRxiv - Immunology 2022Quote: ... The antibody cocktails included anti-mouse antibodies from Miltenyi Biotec: CD44-FITC (clone IM7.8.1) ...
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bioRxiv - Immunology 2022Quote: ... 0.5% FBS and 0.09% sodium azide) with a 100µm smart strainer (Miltenyi Biotec). 10% of the spleen was subjected to red blood cell lysis (eBioscience) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plated neurospheres were maintained for 7 days on N2B27 basal medium supplemented with Dual Smad inhibitors (Miltenyi Biotec (SB), Tocris (DM)) ...
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bioRxiv - Molecular Biology 2022Quote: ... the human induced pluripotent stem cell (hiPSC) line HMGU12 was harvested using StemMACS Passaging Solution (Miltenyi Biotec) and resuspended in DMEM/F-12 medium supplemented with 20% KSR ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM purmorphamine (Miltenyi Biotec), and 10 μM Y-27632 (Bio-Techne) ...
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bioRxiv - Immunology 2022Quote: ... 2 mM EDTA in PBS) and CD31+ and CD45+ cells were sequentially positively selected using anti-CD31 and anti-CD45 MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2022Quote: Whole bone marrow was obtained (AllCells) and CD34+ cells were selected using CD34 MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... minced and dissociated using the gentleMACS Octo Dissociator (Miltenyi Biotec) and the relevant kit (Tumor Dissociation Kit mouse) ...
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bioRxiv - Cell Biology 2022Quote: ... and magnet purification using LS columns (Miltenyi Biotec) and a MACS magnet ...
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bioRxiv - Immunology 2022Quote: ... single cell suspension of digested thymocytes were depleted of CD45+ cells using CD45 microbeads (Miltenyi Biotec).
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bioRxiv - Bioengineering 2022Quote: Flow cytometry was performed with MACSQuant 10 (Miltenyi Biotec). GFP was measured with the B1 channel and Antares using B2 ...
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bioRxiv - Genomics 2022Quote: ... Dead cells are removed using Dead Cell Removal Kit (Miltenyi Biotec, cat. no. 130-090-101). 1×106 PBMCs were crosslinked in 1 ml freshly prepared glyoxal fixation buffer (3% glyoxal ...
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bioRxiv - Bioengineering 2022Quote: ... The first round of sorting was performed with permissive gates and the enriched population was ~50% double positive for Emerald and EBFP2 as analyzed with MACSQuant VYB (Miltenyi Biotec). This population was sorted again with stricter gates to ~95% purity ...